Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries

AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Alissa Villhauer [verfasserIn] Min Zhu [verfasserIn] Wei Shi [verfasserIn] Xian Jin Xie [verfasserIn] Pamella Hughes [verfasserIn] Amy Lesch [verfasserIn] Karin Weber-Gasparoni [verfasserIn] Justine Kolker [verfasserIn] David Drake [verfasserIn] Jeffrey A. Banas [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	mutans streptococci (MS) oral candida co-colonization acid tolerant bacteria early childhood caries (ECC) caries risk assessment

Übergeordnetes Werk:	In: Frontiers in Dental Medicine - Frontiers Media S.A., 2021, 4(2023)
Übergeordnetes Werk:	volume:4 ; year:2023

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fdmed.2023.991746

Katalog-ID:	DOAJ08831944X

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520			\|a AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development.
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allfields	10.3389/fdmed.2023.991746 doi (DE-627)DOAJ08831944X (DE-599)DOAJ42610434cdc149da917a2108a10d2789 DE-627 ger DE-627 rakwb eng RK1-715 Alissa Villhauer verfasserin aut Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development. mutans streptococci (MS) oral candida co-colonization acid tolerant bacteria early childhood caries (ECC) caries risk assessment Dentistry Min Zhu verfasserin aut Wei Shi verfasserin aut Xian Jin Xie verfasserin aut Pamella Hughes verfasserin aut Amy Lesch verfasserin aut Karin Weber-Gasparoni verfasserin aut Justine Kolker verfasserin aut David Drake verfasserin aut Jeffrey A. Banas verfasserin aut In Frontiers in Dental Medicine Frontiers Media S.A., 2021 4(2023) (DE-627)1699959161 (DE-600)3025424-3 26734915 nnns volume:4 year:2023 https://doi.org/10.3389/fdmed.2023.991746 kostenfrei https://doaj.org/article/42610434cdc149da917a2108a10d2789 kostenfrei https://www.frontiersin.org/articles/10.3389/fdmed.2023.991746/full kostenfrei https://doaj.org/toc/2673-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2023
spelling	10.3389/fdmed.2023.991746 doi (DE-627)DOAJ08831944X (DE-599)DOAJ42610434cdc149da917a2108a10d2789 DE-627 ger DE-627 rakwb eng RK1-715 Alissa Villhauer verfasserin aut Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development. mutans streptococci (MS) oral candida co-colonization acid tolerant bacteria early childhood caries (ECC) caries risk assessment Dentistry Min Zhu verfasserin aut Wei Shi verfasserin aut Xian Jin Xie verfasserin aut Pamella Hughes verfasserin aut Amy Lesch verfasserin aut Karin Weber-Gasparoni verfasserin aut Justine Kolker verfasserin aut David Drake verfasserin aut Jeffrey A. Banas verfasserin aut In Frontiers in Dental Medicine Frontiers Media S.A., 2021 4(2023) (DE-627)1699959161 (DE-600)3025424-3 26734915 nnns volume:4 year:2023 https://doi.org/10.3389/fdmed.2023.991746 kostenfrei https://doaj.org/article/42610434cdc149da917a2108a10d2789 kostenfrei https://www.frontiersin.org/articles/10.3389/fdmed.2023.991746/full kostenfrei https://doaj.org/toc/2673-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2023
allfields_unstemmed	10.3389/fdmed.2023.991746 doi (DE-627)DOAJ08831944X (DE-599)DOAJ42610434cdc149da917a2108a10d2789 DE-627 ger DE-627 rakwb eng RK1-715 Alissa Villhauer verfasserin aut Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development. mutans streptococci (MS) oral candida co-colonization acid tolerant bacteria early childhood caries (ECC) caries risk assessment Dentistry Min Zhu verfasserin aut Wei Shi verfasserin aut Xian Jin Xie verfasserin aut Pamella Hughes verfasserin aut Amy Lesch verfasserin aut Karin Weber-Gasparoni verfasserin aut Justine Kolker verfasserin aut David Drake verfasserin aut Jeffrey A. Banas verfasserin aut In Frontiers in Dental Medicine Frontiers Media S.A., 2021 4(2023) (DE-627)1699959161 (DE-600)3025424-3 26734915 nnns volume:4 year:2023 https://doi.org/10.3389/fdmed.2023.991746 kostenfrei https://doaj.org/article/42610434cdc149da917a2108a10d2789 kostenfrei https://www.frontiersin.org/articles/10.3389/fdmed.2023.991746/full kostenfrei https://doaj.org/toc/2673-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2023
allfieldsGer	10.3389/fdmed.2023.991746 doi (DE-627)DOAJ08831944X (DE-599)DOAJ42610434cdc149da917a2108a10d2789 DE-627 ger DE-627 rakwb eng RK1-715 Alissa Villhauer verfasserin aut Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development. mutans streptococci (MS) oral candida co-colonization acid tolerant bacteria early childhood caries (ECC) caries risk assessment Dentistry Min Zhu verfasserin aut Wei Shi verfasserin aut Xian Jin Xie verfasserin aut Pamella Hughes verfasserin aut Amy Lesch verfasserin aut Karin Weber-Gasparoni verfasserin aut Justine Kolker verfasserin aut David Drake verfasserin aut Jeffrey A. Banas verfasserin aut In Frontiers in Dental Medicine Frontiers Media S.A., 2021 4(2023) (DE-627)1699959161 (DE-600)3025424-3 26734915 nnns volume:4 year:2023 https://doi.org/10.3389/fdmed.2023.991746 kostenfrei https://doaj.org/article/42610434cdc149da917a2108a10d2789 kostenfrei https://www.frontiersin.org/articles/10.3389/fdmed.2023.991746/full kostenfrei https://doaj.org/toc/2673-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2023
allfieldsSound	10.3389/fdmed.2023.991746 doi (DE-627)DOAJ08831944X (DE-599)DOAJ42610434cdc149da917a2108a10d2789 DE-627 ger DE-627 rakwb eng RK1-715 Alissa Villhauer verfasserin aut Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development. mutans streptococci (MS) oral candida co-colonization acid tolerant bacteria early childhood caries (ECC) caries risk assessment Dentistry Min Zhu verfasserin aut Wei Shi verfasserin aut Xian Jin Xie verfasserin aut Pamella Hughes verfasserin aut Amy Lesch verfasserin aut Karin Weber-Gasparoni verfasserin aut Justine Kolker verfasserin aut David Drake verfasserin aut Jeffrey A. Banas verfasserin aut In Frontiers in Dental Medicine Frontiers Media S.A., 2021 4(2023) (DE-627)1699959161 (DE-600)3025424-3 26734915 nnns volume:4 year:2023 https://doi.org/10.3389/fdmed.2023.991746 kostenfrei https://doaj.org/article/42610434cdc149da917a2108a10d2789 kostenfrei https://www.frontiersin.org/articles/10.3389/fdmed.2023.991746/full kostenfrei https://doaj.org/toc/2673-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2023
language	English
source	In Frontiers in Dental Medicine 4(2023) volume:4 year:2023
sourceStr	In Frontiers in Dental Medicine 4(2023) volume:4 year:2023
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institution	findex.gbv.de
topic_facet	mutans streptococci (MS) oral candida co-colonization acid tolerant bacteria early childhood caries (ECC) caries risk assessment Dentistry
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container_title	Frontiers in Dental Medicine
authorswithroles_txt_mv	Alissa Villhauer @@aut@@ Min Zhu @@aut@@ Wei Shi @@aut@@ Xian Jin Xie @@aut@@ Pamella Hughes @@aut@@ Amy Lesch @@aut@@ Karin Weber-Gasparoni @@aut@@ Justine Kolker @@aut@@ David Drake @@aut@@ Jeffrey A. Banas @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
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This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p &lt; 0.001) and have Candida species present (p &lt; 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p &lt; 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. 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callnumber-first	R - Medicine
author	Alissa Villhauer
spellingShingle	Alissa Villhauer misc RK1-715 misc mutans streptococci (MS) misc oral candida misc co-colonization misc acid tolerant bacteria misc early childhood caries (ECC) misc caries risk assessment misc Dentistry Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries
authorStr	Alissa Villhauer
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topic_title	RK1-715 Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries mutans streptococci (MS) oral candida co-colonization acid tolerant bacteria early childhood caries (ECC) caries risk assessment
topic	misc RK1-715 misc mutans streptococci (MS) misc oral candida misc co-colonization misc acid tolerant bacteria misc early childhood caries (ECC) misc caries risk assessment misc Dentistry
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title	Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries
ctrlnum	(DE-627)DOAJ08831944X (DE-599)DOAJ42610434cdc149da917a2108a10d2789
title_full	Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries
author_sort	Alissa Villhauer
journal	Frontiers in Dental Medicine
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author_browse	Alissa Villhauer Min Zhu Wei Shi Xian Jin Xie Pamella Hughes Amy Lesch Karin Weber-Gasparoni Justine Kolker David Drake Jeffrey A. Banas
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title_sort	role of mutans streptococci, acid tolerant bacteria and oral candida species in predicting the onset of early childhood caries
callnumber	RK1-715
title_auth	Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries
abstract	AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development.
abstractGer	AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development.
abstract_unstemmed	AimEarly childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species.MethodsChildren between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment.ResultsChildren who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development.ConclusionIn children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development.
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title_short	Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries
url	https://doi.org/10.3389/fdmed.2023.991746 https://doaj.org/article/42610434cdc149da917a2108a10d2789 https://www.frontiersin.org/articles/10.3389/fdmed.2023.991746/full https://doaj.org/toc/2673-4915
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author2	Min Zhu Wei Shi Xian Jin Xie Pamella Hughes Amy Lesch Karin Weber-Gasparoni Justine Kolker David Drake Jeffrey A. Banas
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