Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism

IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Chongshan Yuan [verfasserIn] Kaiyan Zhang [verfasserIn] Zhe Wang [verfasserIn] Xin Ma [verfasserIn] Hongyu Liu [verfasserIn] Jing Zhao [verfasserIn] Wenfa Lu [verfasserIn] Jun Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	cryopreservation bull semen flaxseed oil vitamin E metabolomics transcriptomics

Übergeordnetes Werk:	In: Frontiers in Endocrinology - Frontiers Media S.A., 2011, 14(2023)
Übergeordnetes Werk:	volume:14 ; year:2023

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fendo.2023.1139725

Katalog-ID:	DOAJ089302001

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245	1	0	\|a Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism
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520			\|a IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.
650		4	\|a cryopreservation
650		4	\|a bull semen
650		4	\|a flaxseed oil
650		4	\|a vitamin E
650		4	\|a metabolomics
650		4	\|a transcriptomics
653		0	\|a Diseases of the endocrine glands. Clinical endocrinology
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700	0		\|a Kaiyan Zhang \|e verfasserin \|4 aut
700	0		\|a Kaiyan Zhang \|e verfasserin \|4 aut
700	0		\|a Zhe Wang \|e verfasserin \|4 aut
700	0		\|a Zhe Wang \|e verfasserin \|4 aut
700	0		\|a Xin Ma \|e verfasserin \|4 aut
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700	0		\|a Wenfa Lu \|e verfasserin \|4 aut
700	0		\|a Wenfa Lu \|e verfasserin \|4 aut
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700	0		\|a Jun Wang \|e verfasserin \|4 aut
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allfields	10.3389/fendo.2023.1139725 doi (DE-627)DOAJ089302001 (DE-599)DOAJ57f594ebe1e1427bb13a9f7c770682d2 DE-627 ger DE-627 rakwb eng RC648-665 Chongshan Yuan verfasserin aut Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway. cryopreservation bull semen flaxseed oil vitamin E metabolomics transcriptomics Diseases of the endocrine glands. Clinical endocrinology Chongshan Yuan verfasserin aut Kaiyan Zhang verfasserin aut Kaiyan Zhang verfasserin aut Zhe Wang verfasserin aut Zhe Wang verfasserin aut Xin Ma verfasserin aut Xin Ma verfasserin aut Hongyu Liu verfasserin aut Hongyu Liu verfasserin aut Jing Zhao verfasserin aut Jing Zhao verfasserin aut Wenfa Lu verfasserin aut Wenfa Lu verfasserin aut Jun Wang verfasserin aut Jun Wang verfasserin aut In Frontiers in Endocrinology Frontiers Media S.A., 2011 14(2023) (DE-627)645090948 (DE-600)2592084-4 16642392 nnns volume:14 year:2023 https://doi.org/10.3389/fendo.2023.1139725 kostenfrei https://doaj.org/article/57f594ebe1e1427bb13a9f7c770682d2 kostenfrei https://www.frontiersin.org/articles/10.3389/fendo.2023.1139725/full kostenfrei https://doaj.org/toc/1664-2392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
spelling	10.3389/fendo.2023.1139725 doi (DE-627)DOAJ089302001 (DE-599)DOAJ57f594ebe1e1427bb13a9f7c770682d2 DE-627 ger DE-627 rakwb eng RC648-665 Chongshan Yuan verfasserin aut Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway. cryopreservation bull semen flaxseed oil vitamin E metabolomics transcriptomics Diseases of the endocrine glands. Clinical endocrinology Chongshan Yuan verfasserin aut Kaiyan Zhang verfasserin aut Kaiyan Zhang verfasserin aut Zhe Wang verfasserin aut Zhe Wang verfasserin aut Xin Ma verfasserin aut Xin Ma verfasserin aut Hongyu Liu verfasserin aut Hongyu Liu verfasserin aut Jing Zhao verfasserin aut Jing Zhao verfasserin aut Wenfa Lu verfasserin aut Wenfa Lu verfasserin aut Jun Wang verfasserin aut Jun Wang verfasserin aut In Frontiers in Endocrinology Frontiers Media S.A., 2011 14(2023) (DE-627)645090948 (DE-600)2592084-4 16642392 nnns volume:14 year:2023 https://doi.org/10.3389/fendo.2023.1139725 kostenfrei https://doaj.org/article/57f594ebe1e1427bb13a9f7c770682d2 kostenfrei https://www.frontiersin.org/articles/10.3389/fendo.2023.1139725/full kostenfrei https://doaj.org/toc/1664-2392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfields_unstemmed	10.3389/fendo.2023.1139725 doi (DE-627)DOAJ089302001 (DE-599)DOAJ57f594ebe1e1427bb13a9f7c770682d2 DE-627 ger DE-627 rakwb eng RC648-665 Chongshan Yuan verfasserin aut Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway. cryopreservation bull semen flaxseed oil vitamin E metabolomics transcriptomics Diseases of the endocrine glands. Clinical endocrinology Chongshan Yuan verfasserin aut Kaiyan Zhang verfasserin aut Kaiyan Zhang verfasserin aut Zhe Wang verfasserin aut Zhe Wang verfasserin aut Xin Ma verfasserin aut Xin Ma verfasserin aut Hongyu Liu verfasserin aut Hongyu Liu verfasserin aut Jing Zhao verfasserin aut Jing Zhao verfasserin aut Wenfa Lu verfasserin aut Wenfa Lu verfasserin aut Jun Wang verfasserin aut Jun Wang verfasserin aut In Frontiers in Endocrinology Frontiers Media S.A., 2011 14(2023) (DE-627)645090948 (DE-600)2592084-4 16642392 nnns volume:14 year:2023 https://doi.org/10.3389/fendo.2023.1139725 kostenfrei https://doaj.org/article/57f594ebe1e1427bb13a9f7c770682d2 kostenfrei https://www.frontiersin.org/articles/10.3389/fendo.2023.1139725/full kostenfrei https://doaj.org/toc/1664-2392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfieldsGer	10.3389/fendo.2023.1139725 doi (DE-627)DOAJ089302001 (DE-599)DOAJ57f594ebe1e1427bb13a9f7c770682d2 DE-627 ger DE-627 rakwb eng RC648-665 Chongshan Yuan verfasserin aut Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway. cryopreservation bull semen flaxseed oil vitamin E metabolomics transcriptomics Diseases of the endocrine glands. Clinical endocrinology Chongshan Yuan verfasserin aut Kaiyan Zhang verfasserin aut Kaiyan Zhang verfasserin aut Zhe Wang verfasserin aut Zhe Wang verfasserin aut Xin Ma verfasserin aut Xin Ma verfasserin aut Hongyu Liu verfasserin aut Hongyu Liu verfasserin aut Jing Zhao verfasserin aut Jing Zhao verfasserin aut Wenfa Lu verfasserin aut Wenfa Lu verfasserin aut Jun Wang verfasserin aut Jun Wang verfasserin aut In Frontiers in Endocrinology Frontiers Media S.A., 2011 14(2023) (DE-627)645090948 (DE-600)2592084-4 16642392 nnns volume:14 year:2023 https://doi.org/10.3389/fendo.2023.1139725 kostenfrei https://doaj.org/article/57f594ebe1e1427bb13a9f7c770682d2 kostenfrei https://www.frontiersin.org/articles/10.3389/fendo.2023.1139725/full kostenfrei https://doaj.org/toc/1664-2392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
allfieldsSound	10.3389/fendo.2023.1139725 doi (DE-627)DOAJ089302001 (DE-599)DOAJ57f594ebe1e1427bb13a9f7c770682d2 DE-627 ger DE-627 rakwb eng RC648-665 Chongshan Yuan verfasserin aut Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway. cryopreservation bull semen flaxseed oil vitamin E metabolomics transcriptomics Diseases of the endocrine glands. Clinical endocrinology Chongshan Yuan verfasserin aut Kaiyan Zhang verfasserin aut Kaiyan Zhang verfasserin aut Zhe Wang verfasserin aut Zhe Wang verfasserin aut Xin Ma verfasserin aut Xin Ma verfasserin aut Hongyu Liu verfasserin aut Hongyu Liu verfasserin aut Jing Zhao verfasserin aut Jing Zhao verfasserin aut Wenfa Lu verfasserin aut Wenfa Lu verfasserin aut Jun Wang verfasserin aut Jun Wang verfasserin aut In Frontiers in Endocrinology Frontiers Media S.A., 2011 14(2023) (DE-627)645090948 (DE-600)2592084-4 16642392 nnns volume:14 year:2023 https://doi.org/10.3389/fendo.2023.1139725 kostenfrei https://doaj.org/article/57f594ebe1e1427bb13a9f7c770682d2 kostenfrei https://www.frontiersin.org/articles/10.3389/fendo.2023.1139725/full kostenfrei https://doaj.org/toc/1664-2392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023
language	English
source	In Frontiers in Endocrinology 14(2023) volume:14 year:2023
sourceStr	In Frontiers in Endocrinology 14(2023) volume:14 year:2023
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	cryopreservation bull semen flaxseed oil vitamin E metabolomics transcriptomics Diseases of the endocrine glands. Clinical endocrinology
isfreeaccess_bool	true
container_title	Frontiers in Endocrinology
authorswithroles_txt_mv	Chongshan Yuan @@aut@@ Kaiyan Zhang @@aut@@ Zhe Wang @@aut@@ Xin Ma @@aut@@ Hongyu Liu @@aut@@ Jing Zhao @@aut@@ Wenfa Lu @@aut@@ Jun Wang @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	645090948
id	DOAJ089302001
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">DOAJ089302001</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230505005510.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230505s2023 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3389/fendo.2023.1139725</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ089302001</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ57f594ebe1e1427bb13a9f7c770682d2</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC648-665</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Chongshan Yuan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P &lt; 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P &lt; 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P &lt; 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P &lt; 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P &lt; 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">cryopreservation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bull semen</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">flaxseed oil</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">vitamin E</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">metabolomics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">transcriptomics</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Diseases of the endocrine glands. Clinical endocrinology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chongshan Yuan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Kaiyan Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Kaiyan Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Zhe Wang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Zhe Wang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xin Ma</subfield><subfield code="e">verfasserin</subfield><subfield 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callnumber-first	R - Medicine
author	Chongshan Yuan
spellingShingle	Chongshan Yuan misc RC648-665 misc cryopreservation misc bull semen misc flaxseed oil misc vitamin E misc metabolomics misc transcriptomics misc Diseases of the endocrine glands. Clinical endocrinology Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism
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topic_title	RC648-665 Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism cryopreservation bull semen flaxseed oil vitamin E metabolomics transcriptomics
topic	misc RC648-665 misc cryopreservation misc bull semen misc flaxseed oil misc vitamin E misc metabolomics misc transcriptomics misc Diseases of the endocrine glands. Clinical endocrinology
topic_unstemmed	misc RC648-665 misc cryopreservation misc bull semen misc flaxseed oil misc vitamin E misc metabolomics misc transcriptomics misc Diseases of the endocrine glands. Clinical endocrinology
topic_browse	misc RC648-665 misc cryopreservation misc bull semen misc flaxseed oil misc vitamin E misc metabolomics misc transcriptomics misc Diseases of the endocrine glands. Clinical endocrinology
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title	Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism
ctrlnum	(DE-627)DOAJ089302001 (DE-599)DOAJ57f594ebe1e1427bb13a9f7c770682d2
title_full	Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism
author_sort	Chongshan Yuan
journal	Frontiers in Endocrinology
journalStr	Frontiers in Endocrinology
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author_browse	Chongshan Yuan Kaiyan Zhang Zhe Wang Xin Ma Hongyu Liu Jing Zhao Wenfa Lu Jun Wang
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author-letter	Chongshan Yuan
doi_str_mv	10.3389/fendo.2023.1139725
author2-role	verfasserin
title_sort	dietary flaxseed oil and vitamin e improve semen quality via propionic acid metabolism
callnumber	RC648-665
title_auth	Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism
abstract	IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.
abstractGer	IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.
abstract_unstemmed	IntroductionFlaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.
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title_short	Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism
url	https://doi.org/10.3389/fendo.2023.1139725 https://doaj.org/article/57f594ebe1e1427bb13a9f7c770682d2 https://www.frontiersin.org/articles/10.3389/fendo.2023.1139725/full https://doaj.org/toc/1664-2392
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author2	Chongshan Yuan Kaiyan Zhang Zhe Wang Xin Ma Hongyu Liu Jing Zhao Wenfa Lu Jun Wang
author2Str	Chongshan Yuan Kaiyan Zhang Zhe Wang Xin Ma Hongyu Liu Jing Zhao Wenfa Lu Jun Wang
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The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.Methods16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.ResultsThe results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P &lt; 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P &lt; 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P &lt; 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P &lt; 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P &lt; 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">cryopreservation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bull semen</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">flaxseed oil</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">vitamin E</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">metabolomics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">transcriptomics</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Diseases of the endocrine glands. 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