Identification of an adipose tissue-resident pro-preadipocyte population

Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them i...
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Autor*in:	Min Chen [verfasserIn] Soochi Kim [verfasserIn] Liang Li [verfasserIn] Sourav Chattopadhyay [verfasserIn] Thomas A. Rando [verfasserIn] Brian J. Feldman [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	CP: Stem cell research CP: Metabolism

Übergeordnetes Werk:	In: Cell Reports - Elsevier, 2015, 42(2023), 5, Seite 112440-
Übergeordnetes Werk:	volume:42 ; year:2023 ; number:5 ; pages:112440-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.celrep.2023.112440

Katalog-ID:	DOAJ089705963

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publishDate	2023
allfields	10.1016/j.celrep.2023.112440 doi (DE-627)DOAJ089705963 (DE-599)DOAJe6f44d1ac4cf47919cada604504e4212 DE-627 ger DE-627 rakwb eng QH301-705.5 Min Chen verfasserin aut Identification of an adipose tissue-resident pro-preadipocyte population 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis. CP: Stem cell research CP: Metabolism Biology (General) Soochi Kim verfasserin aut Liang Li verfasserin aut Sourav Chattopadhyay verfasserin aut Thomas A. Rando verfasserin aut Brian J. Feldman verfasserin aut In Cell Reports Elsevier, 2015 42(2023), 5, Seite 112440- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:42 year:2023 number:5 pages:112440- https://doi.org/10.1016/j.celrep.2023.112440 kostenfrei https://doaj.org/article/e6f44d1ac4cf47919cada604504e4212 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124723004515 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 42 2023 5 112440-
spelling	10.1016/j.celrep.2023.112440 doi (DE-627)DOAJ089705963 (DE-599)DOAJe6f44d1ac4cf47919cada604504e4212 DE-627 ger DE-627 rakwb eng QH301-705.5 Min Chen verfasserin aut Identification of an adipose tissue-resident pro-preadipocyte population 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis. CP: Stem cell research CP: Metabolism Biology (General) Soochi Kim verfasserin aut Liang Li verfasserin aut Sourav Chattopadhyay verfasserin aut Thomas A. Rando verfasserin aut Brian J. Feldman verfasserin aut In Cell Reports Elsevier, 2015 42(2023), 5, Seite 112440- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:42 year:2023 number:5 pages:112440- https://doi.org/10.1016/j.celrep.2023.112440 kostenfrei https://doaj.org/article/e6f44d1ac4cf47919cada604504e4212 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124723004515 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 42 2023 5 112440-
allfields_unstemmed	10.1016/j.celrep.2023.112440 doi (DE-627)DOAJ089705963 (DE-599)DOAJe6f44d1ac4cf47919cada604504e4212 DE-627 ger DE-627 rakwb eng QH301-705.5 Min Chen verfasserin aut Identification of an adipose tissue-resident pro-preadipocyte population 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis. CP: Stem cell research CP: Metabolism Biology (General) Soochi Kim verfasserin aut Liang Li verfasserin aut Sourav Chattopadhyay verfasserin aut Thomas A. Rando verfasserin aut Brian J. Feldman verfasserin aut In Cell Reports Elsevier, 2015 42(2023), 5, Seite 112440- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:42 year:2023 number:5 pages:112440- https://doi.org/10.1016/j.celrep.2023.112440 kostenfrei https://doaj.org/article/e6f44d1ac4cf47919cada604504e4212 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124723004515 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 42 2023 5 112440-
allfieldsGer	10.1016/j.celrep.2023.112440 doi (DE-627)DOAJ089705963 (DE-599)DOAJe6f44d1ac4cf47919cada604504e4212 DE-627 ger DE-627 rakwb eng QH301-705.5 Min Chen verfasserin aut Identification of an adipose tissue-resident pro-preadipocyte population 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis. CP: Stem cell research CP: Metabolism Biology (General) Soochi Kim verfasserin aut Liang Li verfasserin aut Sourav Chattopadhyay verfasserin aut Thomas A. Rando verfasserin aut Brian J. Feldman verfasserin aut In Cell Reports Elsevier, 2015 42(2023), 5, Seite 112440- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:42 year:2023 number:5 pages:112440- https://doi.org/10.1016/j.celrep.2023.112440 kostenfrei https://doaj.org/article/e6f44d1ac4cf47919cada604504e4212 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124723004515 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 42 2023 5 112440-
allfieldsSound	10.1016/j.celrep.2023.112440 doi (DE-627)DOAJ089705963 (DE-599)DOAJe6f44d1ac4cf47919cada604504e4212 DE-627 ger DE-627 rakwb eng QH301-705.5 Min Chen verfasserin aut Identification of an adipose tissue-resident pro-preadipocyte population 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis. CP: Stem cell research CP: Metabolism Biology (General) Soochi Kim verfasserin aut Liang Li verfasserin aut Sourav Chattopadhyay verfasserin aut Thomas A. Rando verfasserin aut Brian J. Feldman verfasserin aut In Cell Reports Elsevier, 2015 42(2023), 5, Seite 112440- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:42 year:2023 number:5 pages:112440- https://doi.org/10.1016/j.celrep.2023.112440 kostenfrei https://doaj.org/article/e6f44d1ac4cf47919cada604504e4212 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124723004515 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 42 2023 5 112440-
language	English
source	In Cell Reports 42(2023), 5, Seite 112440- volume:42 year:2023 number:5 pages:112440-
sourceStr	In Cell Reports 42(2023), 5, Seite 112440- volume:42 year:2023 number:5 pages:112440-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CP: Stem cell research CP: Metabolism Biology (General)
isfreeaccess_bool	true
container_title	Cell Reports
authorswithroles_txt_mv	Min Chen @@aut@@ Soochi Kim @@aut@@ Liang Li @@aut@@ Sourav Chattopadhyay @@aut@@ Thomas A. Rando @@aut@@ Brian J. Feldman @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	684964562
id	DOAJ089705963
language_de	englisch
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callnumber-first	Q - Science
author	Min Chen
spellingShingle	Min Chen misc QH301-705.5 misc CP: Stem cell research misc CP: Metabolism misc Biology (General) Identification of an adipose tissue-resident pro-preadipocyte population
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topic_title	QH301-705.5 Identification of an adipose tissue-resident pro-preadipocyte population CP: Stem cell research CP: Metabolism
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title	Identification of an adipose tissue-resident pro-preadipocyte population
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title_full	Identification of an adipose tissue-resident pro-preadipocyte population
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author_browse	Min Chen Soochi Kim Liang Li Sourav Chattopadhyay Thomas A. Rando Brian J. Feldman
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title_sort	identification of an adipose tissue-resident pro-preadipocyte population
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title_auth	Identification of an adipose tissue-resident pro-preadipocyte population
abstract	Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis.
abstractGer	Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis.
abstract_unstemmed	Summary: Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis.
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title_short	Identification of an adipose tissue-resident pro-preadipocyte population
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Identification of an adipose tissue-resident pro-preadipocyte population

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