<i<Pneumocystis jirovecii</i< Pneumonia Diagnostic Approach: Real-Life Experience in a Tertiary Centre

<i<Pneumocystis jirovecii</i< pneumonia (PJP) in immunocompromised patients entails high mortality and requires adequate laboratory diagnosis. We compared the performance of a real time-PCR assay against the immunofluorescence assay (IFA) in the routine of a large microbiology laboratory...
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Gespeichert in:

Autor*in:	Cristina Veintimilla [verfasserIn] Ana Álvarez-Uría [verfasserIn] Pablo Martín-Rabadán [verfasserIn] Maricela Valerio [verfasserIn] Marina Machado [verfasserIn] Belén Padilla [verfasserIn] Roberto Alonso [verfasserIn] Cristina Diez [verfasserIn] Patricia Muñoz [verfasserIn] Mercedes Marín [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	indirect immunofluorescence staining polymerase chain reaction (PCR) diagnostic accuracy immunocompromised host

Übergeordnetes Werk:	In: Journal of Fungi - MDPI AG, 2015, 9(2023), 4, p 414
Übergeordnetes Werk:	volume:9 ; year:2023 ; number:4, p 414

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/jof9040414

Katalog-ID:	DOAJ089830768

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allfieldsSound	10.3390/jof9040414 doi (DE-627)DOAJ089830768 (DE-599)DOAJ4c95503f80b04346aea579bf77457c94 DE-627 ger DE-627 rakwb eng QH301-705.5 Cristina Veintimilla verfasserin aut <i<Pneumocystis jirovecii</i< Pneumonia Diagnostic Approach: Real-Life Experience in a Tertiary Centre 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Pneumocystis jirovecii</i< pneumonia (PJP) in immunocompromised patients entails high mortality and requires adequate laboratory diagnosis. We compared the performance of a real time-PCR assay against the immunofluorescence assay (IFA) in the routine of a large microbiology laboratory. Different respiratory samples from HIV and non-HIV-infected patients were included. The retrospective analysis used data from September 2015 to April 2018, which included all samples for which a <i<P. jirovecii</i< test was requested. A total of 299 respiratory samples were tested (bronchoalveolar lavage fluid (<i<n</i< = 181), tracheal aspirate (<i<n</i< = 53) and sputum (<i<n</i< = 65)). Forty-eight (16.1%) patients fulfilled the criteria for PJP. Five positive samples (10%) had only colonization. The PCR test was found to have a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 96%, 98%, 90% and 99%, compared to 27%, 100%, 100% and 87%, for the IFA, respectively. PJ-PCR sensitivity and specificity were <80% and <90% for all tested respiratory samples. Median cycle threshold values in definite PJP cases were 30 versus 37 in colonized cases (<i<p</i< < 0.05). Thus, the PCR assay is a robust and reliable test for the diagnosis PJP in all respiratory sample types. Ct values of ≥36 could help to exclude PJP diagnosis. <i<Pneumocystis jirovecii</i< pneumonia indirect immunofluorescence staining polymerase chain reaction (PCR) diagnostic accuracy immunocompromised host Biology (General) Ana Álvarez-Uría verfasserin aut Pablo Martín-Rabadán verfasserin aut Maricela Valerio verfasserin aut Marina Machado verfasserin aut Belén Padilla verfasserin aut Roberto Alonso verfasserin aut Cristina Diez verfasserin aut Patricia Muñoz verfasserin aut Mercedes Marín verfasserin aut In Journal of Fungi MDPI AG, 2015 9(2023), 4, p 414 (DE-627)796588538 (DE-600)2784229-0 2309608X nnns volume:9 year:2023 number:4, p 414 https://doi.org/10.3390/jof9040414 kostenfrei https://doaj.org/article/4c95503f80b04346aea579bf77457c94 kostenfrei https://www.mdpi.com/2309-608X/9/4/414 kostenfrei https://doaj.org/toc/2309-608X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 4, p 414
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author	Cristina Veintimilla
spellingShingle	Cristina Veintimilla misc QH301-705.5 misc <i<Pneumocystis jirovecii</i< pneumonia misc indirect immunofluorescence misc staining misc polymerase chain reaction (PCR) misc diagnostic accuracy misc immunocompromised host misc Biology (General) <i<Pneumocystis jirovecii</i< Pneumonia Diagnostic Approach: Real-Life Experience in a Tertiary Centre
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topic_title	QH301-705.5 <i<Pneumocystis jirovecii</i< Pneumonia Diagnostic Approach: Real-Life Experience in a Tertiary Centre <i<Pneumocystis jirovecii</i< pneumonia indirect immunofluorescence staining polymerase chain reaction (PCR) diagnostic accuracy immunocompromised host
topic	misc QH301-705.5 misc <i<Pneumocystis jirovecii</i< pneumonia misc indirect immunofluorescence misc staining misc polymerase chain reaction (PCR) misc diagnostic accuracy misc immunocompromised host misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc <i<Pneumocystis jirovecii</i< pneumonia misc indirect immunofluorescence misc staining misc polymerase chain reaction (PCR) misc diagnostic accuracy misc immunocompromised host misc Biology (General)
topic_browse	misc QH301-705.5 misc <i<Pneumocystis jirovecii</i< pneumonia misc indirect immunofluorescence misc staining misc polymerase chain reaction (PCR) misc diagnostic accuracy misc immunocompromised host misc Biology (General)
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title_auth	<i<Pneumocystis jirovecii</i< Pneumonia Diagnostic Approach: Real-Life Experience in a Tertiary Centre
abstract	<i<Pneumocystis jirovecii</i< pneumonia (PJP) in immunocompromised patients entails high mortality and requires adequate laboratory diagnosis. We compared the performance of a real time-PCR assay against the immunofluorescence assay (IFA) in the routine of a large microbiology laboratory. Different respiratory samples from HIV and non-HIV-infected patients were included. The retrospective analysis used data from September 2015 to April 2018, which included all samples for which a <i<P. jirovecii</i< test was requested. A total of 299 respiratory samples were tested (bronchoalveolar lavage fluid (<i<n</i< = 181), tracheal aspirate (<i<n</i< = 53) and sputum (<i<n</i< = 65)). Forty-eight (16.1%) patients fulfilled the criteria for PJP. Five positive samples (10%) had only colonization. The PCR test was found to have a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 96%, 98%, 90% and 99%, compared to 27%, 100%, 100% and 87%, for the IFA, respectively. PJ-PCR sensitivity and specificity were <80% and <90% for all tested respiratory samples. Median cycle threshold values in definite PJP cases were 30 versus 37 in colonized cases (<i<p</i< < 0.05). Thus, the PCR assay is a robust and reliable test for the diagnosis PJP in all respiratory sample types. Ct values of ≥36 could help to exclude PJP diagnosis.
abstractGer	<i<Pneumocystis jirovecii</i< pneumonia (PJP) in immunocompromised patients entails high mortality and requires adequate laboratory diagnosis. We compared the performance of a real time-PCR assay against the immunofluorescence assay (IFA) in the routine of a large microbiology laboratory. Different respiratory samples from HIV and non-HIV-infected patients were included. The retrospective analysis used data from September 2015 to April 2018, which included all samples for which a <i<P. jirovecii</i< test was requested. A total of 299 respiratory samples were tested (bronchoalveolar lavage fluid (<i<n</i< = 181), tracheal aspirate (<i<n</i< = 53) and sputum (<i<n</i< = 65)). Forty-eight (16.1%) patients fulfilled the criteria for PJP. Five positive samples (10%) had only colonization. The PCR test was found to have a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 96%, 98%, 90% and 99%, compared to 27%, 100%, 100% and 87%, for the IFA, respectively. PJ-PCR sensitivity and specificity were <80% and <90% for all tested respiratory samples. Median cycle threshold values in definite PJP cases were 30 versus 37 in colonized cases (<i<p</i< < 0.05). Thus, the PCR assay is a robust and reliable test for the diagnosis PJP in all respiratory sample types. Ct values of ≥36 could help to exclude PJP diagnosis.
abstract_unstemmed	<i<Pneumocystis jirovecii</i< pneumonia (PJP) in immunocompromised patients entails high mortality and requires adequate laboratory diagnosis. We compared the performance of a real time-PCR assay against the immunofluorescence assay (IFA) in the routine of a large microbiology laboratory. Different respiratory samples from HIV and non-HIV-infected patients were included. The retrospective analysis used data from September 2015 to April 2018, which included all samples for which a <i<P. jirovecii</i< test was requested. A total of 299 respiratory samples were tested (bronchoalveolar lavage fluid (<i<n</i< = 181), tracheal aspirate (<i<n</i< = 53) and sputum (<i<n</i< = 65)). Forty-eight (16.1%) patients fulfilled the criteria for PJP. Five positive samples (10%) had only colonization. The PCR test was found to have a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 96%, 98%, 90% and 99%, compared to 27%, 100%, 100% and 87%, for the IFA, respectively. PJ-PCR sensitivity and specificity were <80% and <90% for all tested respiratory samples. Median cycle threshold values in definite PJP cases were 30 versus 37 in colonized cases (<i<p</i< < 0.05). Thus, the PCR assay is a robust and reliable test for the diagnosis PJP in all respiratory sample types. Ct values of ≥36 could help to exclude PJP diagnosis.
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title_short	<i<Pneumocystis jirovecii</i< Pneumonia Diagnostic Approach: Real-Life Experience in a Tertiary Centre
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We compared the performance of a real time-PCR assay against the immunofluorescence assay (IFA) in the routine of a large microbiology laboratory. Different respiratory samples from HIV and non-HIV-infected patients were included. The retrospective analysis used data from September 2015 to April 2018, which included all samples for which a <i<P. jirovecii</i< test was requested. A total of 299 respiratory samples were tested (bronchoalveolar lavage fluid (<i<n</i< = 181), tracheal aspirate (<i<n</i< = 53) and sputum (<i<n</i< = 65)). Forty-eight (16.1%) patients fulfilled the criteria for PJP. Five positive samples (10%) had only colonization. The PCR test was found to have a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 96%, 98%, 90% and 99%, compared to 27%, 100%, 100% and 87%, for the IFA, respectively. PJ-PCR sensitivity and specificity were <80% and <90% for all tested respiratory samples. Median cycle threshold values in definite PJP cases were 30 versus 37 in colonized cases (<i<p</i< < 0.05). Thus, the PCR assay is a robust and reliable test for the diagnosis PJP in all respiratory sample types. 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