Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative

Adult hearts are characterized by inefficient regeneration after injury, thus, the features that support or prevent cardiomyocyte (CM) proliferation are important to clarify. Diploid CMs are a candidate cell type that may have unique proliferative and regenerative competence, but no molecular marker...
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Autor*in:	Hirofumi Watanabe [verfasserIn] Ge Tao [verfasserIn] Peiheng Gan [verfasserIn] Baylee C. Westbury [verfasserIn] Kristie D. Cox [verfasserIn] Kelsey Tjen [verfasserIn] Ruolan Song [verfasserIn] Glenn I. Fishman [verfasserIn] Takako Makita [verfasserIn] Henry M. Sucov [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	polyploidy diploid cardiomyocyte heart regeneration conduction system Purkinje cell

Übergeordnetes Werk:	In: Journal of Cardiovascular Development and Disease - MDPI AG, 2014, 10(2023), 4, p 161
Übergeordnetes Werk:	volume:10 ; year:2023 ; number:4, p 161

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/jcdd10040161

Katalog-ID:	DOAJ089837959

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allfieldsSound	10.3390/jcdd10040161 doi (DE-627)DOAJ089837959 (DE-599)DOAJd8d4c517d1e44ae3b2d7f98118750785 DE-627 ger DE-627 rakwb eng RC666-701 Hirofumi Watanabe verfasserin aut Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Adult hearts are characterized by inefficient regeneration after injury, thus, the features that support or prevent cardiomyocyte (CM) proliferation are important to clarify. Diploid CMs are a candidate cell type that may have unique proliferative and regenerative competence, but no molecular markers are yet known that selectively identify all or subpopulations of diploid CMs. Here, using the conduction system expression marker Cntn2-GFP and the conduction system lineage marker Etv1Cre<sup<ERT2</sup<, we demonstrate that Purkinje CMs that comprise the adult ventricular conduction system are disproportionately diploid (33%, vs. 4% of bulk ventricular CMs). These, however, represent only a small proportion (3%) of the total diploid CM population. Using EdU incorporation during the first postnatal week, we demonstrate that bulk diploid CMs found in the later heart enter and complete the cell cycle during the neonatal period. In contrast, a significant fraction of conduction CMs persist as diploid cells from fetal life and avoid neonatal cell cycle activity. Despite their high degree of diploidy, the Purkinje lineage had no enhanced competence to support regeneration after adult heart infarction. polyploidy diploid cardiomyocyte heart regeneration conduction system Purkinje cell Diseases of the circulatory (Cardiovascular) system Ge Tao verfasserin aut Peiheng Gan verfasserin aut Baylee C. Westbury verfasserin aut Kristie D. Cox verfasserin aut Kelsey Tjen verfasserin aut Ruolan Song verfasserin aut Glenn I. Fishman verfasserin aut Takako Makita verfasserin aut Henry M. Sucov verfasserin aut In Journal of Cardiovascular Development and Disease MDPI AG, 2014 10(2023), 4, p 161 (DE-627)790616017 (DE-600)2777082-5 23083425 nnns volume:10 year:2023 number:4, p 161 https://doi.org/10.3390/jcdd10040161 kostenfrei https://doaj.org/article/d8d4c517d1e44ae3b2d7f98118750785 kostenfrei https://www.mdpi.com/2308-3425/10/4/161 kostenfrei https://doaj.org/toc/2308-3425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023 4, p 161
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callnumber-first	R - Medicine
author	Hirofumi Watanabe
spellingShingle	Hirofumi Watanabe misc RC666-701 misc polyploidy misc diploid cardiomyocyte misc heart regeneration misc conduction system misc Purkinje cell misc Diseases of the circulatory (Cardiovascular) system Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative
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topic_title	RC666-701 Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative polyploidy diploid cardiomyocyte heart regeneration conduction system Purkinje cell
topic	misc RC666-701 misc polyploidy misc diploid cardiomyocyte misc heart regeneration misc conduction system misc Purkinje cell misc Diseases of the circulatory (Cardiovascular) system
topic_unstemmed	misc RC666-701 misc polyploidy misc diploid cardiomyocyte misc heart regeneration misc conduction system misc Purkinje cell misc Diseases of the circulatory (Cardiovascular) system
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title	Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative
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title_full	Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative
author_sort	Hirofumi Watanabe
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author_browse	Hirofumi Watanabe Ge Tao Peiheng Gan Baylee C. Westbury Kristie D. Cox Kelsey Tjen Ruolan Song Glenn I. Fishman Takako Makita Henry M. Sucov
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title_sort	purkinje cardiomyocytes of the adult ventricular conduction system are highly diploid but not uniquely regenerative
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title_auth	Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative
abstract	Adult hearts are characterized by inefficient regeneration after injury, thus, the features that support or prevent cardiomyocyte (CM) proliferation are important to clarify. Diploid CMs are a candidate cell type that may have unique proliferative and regenerative competence, but no molecular markers are yet known that selectively identify all or subpopulations of diploid CMs. Here, using the conduction system expression marker Cntn2-GFP and the conduction system lineage marker Etv1Cre<sup<ERT2</sup<, we demonstrate that Purkinje CMs that comprise the adult ventricular conduction system are disproportionately diploid (33%, vs. 4% of bulk ventricular CMs). These, however, represent only a small proportion (3%) of the total diploid CM population. Using EdU incorporation during the first postnatal week, we demonstrate that bulk diploid CMs found in the later heart enter and complete the cell cycle during the neonatal period. In contrast, a significant fraction of conduction CMs persist as diploid cells from fetal life and avoid neonatal cell cycle activity. Despite their high degree of diploidy, the Purkinje lineage had no enhanced competence to support regeneration after adult heart infarction.
abstractGer	Adult hearts are characterized by inefficient regeneration after injury, thus, the features that support or prevent cardiomyocyte (CM) proliferation are important to clarify. Diploid CMs are a candidate cell type that may have unique proliferative and regenerative competence, but no molecular markers are yet known that selectively identify all or subpopulations of diploid CMs. Here, using the conduction system expression marker Cntn2-GFP and the conduction system lineage marker Etv1Cre<sup<ERT2</sup<, we demonstrate that Purkinje CMs that comprise the adult ventricular conduction system are disproportionately diploid (33%, vs. 4% of bulk ventricular CMs). These, however, represent only a small proportion (3%) of the total diploid CM population. Using EdU incorporation during the first postnatal week, we demonstrate that bulk diploid CMs found in the later heart enter and complete the cell cycle during the neonatal period. In contrast, a significant fraction of conduction CMs persist as diploid cells from fetal life and avoid neonatal cell cycle activity. Despite their high degree of diploidy, the Purkinje lineage had no enhanced competence to support regeneration after adult heart infarction.
abstract_unstemmed	Adult hearts are characterized by inefficient regeneration after injury, thus, the features that support or prevent cardiomyocyte (CM) proliferation are important to clarify. Diploid CMs are a candidate cell type that may have unique proliferative and regenerative competence, but no molecular markers are yet known that selectively identify all or subpopulations of diploid CMs. Here, using the conduction system expression marker Cntn2-GFP and the conduction system lineage marker Etv1Cre<sup<ERT2</sup<, we demonstrate that Purkinje CMs that comprise the adult ventricular conduction system are disproportionately diploid (33%, vs. 4% of bulk ventricular CMs). These, however, represent only a small proportion (3%) of the total diploid CM population. Using EdU incorporation during the first postnatal week, we demonstrate that bulk diploid CMs found in the later heart enter and complete the cell cycle during the neonatal period. In contrast, a significant fraction of conduction CMs persist as diploid cells from fetal life and avoid neonatal cell cycle activity. Despite their high degree of diploidy, the Purkinje lineage had no enhanced competence to support regeneration after adult heart infarction.
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title_short	Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative
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up_date	2024-07-04T00:51:44.911Z
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Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative

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