Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for determining hyaluronic acid in dairy products was proposed in this study. The samples were degraded by hyaluronidase, diluted with acetonitrile, and then purified by PRIME HLB cartridge...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Guohui DU [verfasserIn] Weijiang FAN [verfasserIn] Yujuan CHEN [verfasserIn] Wenwen CHEN [verfasserIn] Liping QIAO [verfasserIn] Cuiling WANG [verfasserIn] |
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| Format: |
E-Artikel |
|---|---|
| Sprache: |
Chinesisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
ultra-high performance liquid chromatography-tandem mass spectrometry |
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| Übergeordnetes Werk: |
In: Shipin gongye ke-ji - The editorial department of Science and Technology of Food Industry, 2022, 44(2023), 8, Seite 334-340 |
|---|---|
| Übergeordnetes Werk: |
volume:44 ; year:2023 ; number:8 ; pages:334-340 |
| Links: |
|---|
| DOI / URN: |
10.13386/j.issn1002-0306.2022070057 |
|---|
| Katalog-ID: |
DOAJ089950593 |
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| 520 | |a The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for determining hyaluronic acid in dairy products was proposed in this study. The samples were degraded by hyaluronidase, diluted with acetonitrile, and then purified by PRIME HLB cartridge. The analysis was conducted on a BEH Amide column (2.1 mm×100 mm, 1.7 μm) by gradient elution using 0.2% concentration of aqueous ammonia solution and acetonitrile (containing 0.2% ammonia) as mobile phases. Qualification was achieved by negative electrospray ionization with multiple reaction monitoring (MRM). Meanwhile, the external standard method for quantitation was applied. In accordance with the results, the recoveries of hyaluronic acid in dairy products were 91.4%~106.2%, and RSDs were 2.3%~6.7% at the addition levels of 0.5~200 mg/kg. Presumably, with a limit of quantification of 0.5 mg/kg, the demonstrated method was easy and feasible for quantifying the hyaluronic acid in dairy products. This method was used to detect commercially dairy products, and the tested samples meet the standard requirements. | ||
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10.13386/j.issn1002-0306.2022070057 doi (DE-627)DOAJ089950593 (DE-599)DOAJ8b478fa0307a4c3d835234f1382f741e DE-627 ger DE-627 rakwb chi TP368-456 Guohui DU verfasserin aut Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for determining hyaluronic acid in dairy products was proposed in this study. The samples were degraded by hyaluronidase, diluted with acetonitrile, and then purified by PRIME HLB cartridge. The analysis was conducted on a BEH Amide column (2.1 mm×100 mm, 1.7 μm) by gradient elution using 0.2% concentration of aqueous ammonia solution and acetonitrile (containing 0.2% ammonia) as mobile phases. Qualification was achieved by negative electrospray ionization with multiple reaction monitoring (MRM). Meanwhile, the external standard method for quantitation was applied. In accordance with the results, the recoveries of hyaluronic acid in dairy products were 91.4%~106.2%, and RSDs were 2.3%~6.7% at the addition levels of 0.5~200 mg/kg. Presumably, with a limit of quantification of 0.5 mg/kg, the demonstrated method was easy and feasible for quantifying the hyaluronic acid in dairy products. This method was used to detect commercially dairy products, and the tested samples meet the standard requirements. ultra-high performance liquid chromatography-tandem mass spectrometry dairy products hyaluronic acid Food processing and manufacture Weijiang FAN verfasserin aut Yujuan CHEN verfasserin aut Wenwen CHEN verfasserin aut Liping QIAO verfasserin aut Cuiling WANG verfasserin aut In Shipin gongye ke-ji The editorial department of Science and Technology of Food Industry, 2022 44(2023), 8, Seite 334-340 (DE-627)DOAJ000150428 10020306 nnns volume:44 year:2023 number:8 pages:334-340 https://doi.org/10.13386/j.issn1002-0306.2022070057 kostenfrei https://doaj.org/article/8b478fa0307a4c3d835234f1382f741e kostenfrei http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070057 kostenfrei https://doaj.org/toc/1002-0306 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 44 2023 8 334-340 |
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10.13386/j.issn1002-0306.2022070057 doi (DE-627)DOAJ089950593 (DE-599)DOAJ8b478fa0307a4c3d835234f1382f741e DE-627 ger DE-627 rakwb chi TP368-456 Guohui DU verfasserin aut Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for determining hyaluronic acid in dairy products was proposed in this study. The samples were degraded by hyaluronidase, diluted with acetonitrile, and then purified by PRIME HLB cartridge. The analysis was conducted on a BEH Amide column (2.1 mm×100 mm, 1.7 μm) by gradient elution using 0.2% concentration of aqueous ammonia solution and acetonitrile (containing 0.2% ammonia) as mobile phases. Qualification was achieved by negative electrospray ionization with multiple reaction monitoring (MRM). Meanwhile, the external standard method for quantitation was applied. In accordance with the results, the recoveries of hyaluronic acid in dairy products were 91.4%~106.2%, and RSDs were 2.3%~6.7% at the addition levels of 0.5~200 mg/kg. Presumably, with a limit of quantification of 0.5 mg/kg, the demonstrated method was easy and feasible for quantifying the hyaluronic acid in dairy products. This method was used to detect commercially dairy products, and the tested samples meet the standard requirements. ultra-high performance liquid chromatography-tandem mass spectrometry dairy products hyaluronic acid Food processing and manufacture Weijiang FAN verfasserin aut Yujuan CHEN verfasserin aut Wenwen CHEN verfasserin aut Liping QIAO verfasserin aut Cuiling WANG verfasserin aut In Shipin gongye ke-ji The editorial department of Science and Technology of Food Industry, 2022 44(2023), 8, Seite 334-340 (DE-627)DOAJ000150428 10020306 nnns volume:44 year:2023 number:8 pages:334-340 https://doi.org/10.13386/j.issn1002-0306.2022070057 kostenfrei https://doaj.org/article/8b478fa0307a4c3d835234f1382f741e kostenfrei http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070057 kostenfrei https://doaj.org/toc/1002-0306 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 44 2023 8 334-340 |
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10.13386/j.issn1002-0306.2022070057 doi (DE-627)DOAJ089950593 (DE-599)DOAJ8b478fa0307a4c3d835234f1382f741e DE-627 ger DE-627 rakwb chi TP368-456 Guohui DU verfasserin aut Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for determining hyaluronic acid in dairy products was proposed in this study. The samples were degraded by hyaluronidase, diluted with acetonitrile, and then purified by PRIME HLB cartridge. The analysis was conducted on a BEH Amide column (2.1 mm×100 mm, 1.7 μm) by gradient elution using 0.2% concentration of aqueous ammonia solution and acetonitrile (containing 0.2% ammonia) as mobile phases. Qualification was achieved by negative electrospray ionization with multiple reaction monitoring (MRM). Meanwhile, the external standard method for quantitation was applied. In accordance with the results, the recoveries of hyaluronic acid in dairy products were 91.4%~106.2%, and RSDs were 2.3%~6.7% at the addition levels of 0.5~200 mg/kg. Presumably, with a limit of quantification of 0.5 mg/kg, the demonstrated method was easy and feasible for quantifying the hyaluronic acid in dairy products. This method was used to detect commercially dairy products, and the tested samples meet the standard requirements. ultra-high performance liquid chromatography-tandem mass spectrometry dairy products hyaluronic acid Food processing and manufacture Weijiang FAN verfasserin aut Yujuan CHEN verfasserin aut Wenwen CHEN verfasserin aut Liping QIAO verfasserin aut Cuiling WANG verfasserin aut In Shipin gongye ke-ji The editorial department of Science and Technology of Food Industry, 2022 44(2023), 8, Seite 334-340 (DE-627)DOAJ000150428 10020306 nnns volume:44 year:2023 number:8 pages:334-340 https://doi.org/10.13386/j.issn1002-0306.2022070057 kostenfrei https://doaj.org/article/8b478fa0307a4c3d835234f1382f741e kostenfrei http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070057 kostenfrei https://doaj.org/toc/1002-0306 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 44 2023 8 334-340 |
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Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
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Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
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Guohui DU |
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Guohui DU Weijiang FAN Yujuan CHEN Wenwen CHEN Liping QIAO Cuiling WANG |
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Elektronische Aufsätze |
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10.13386/j.issn1002-0306.2022070057 |
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determination of hyaluronic acid in dairy products by ultra-high performance liquid chromatography-tandem mass spectrometry |
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TP368-456 |
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Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
| abstract |
The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for determining hyaluronic acid in dairy products was proposed in this study. The samples were degraded by hyaluronidase, diluted with acetonitrile, and then purified by PRIME HLB cartridge. The analysis was conducted on a BEH Amide column (2.1 mm×100 mm, 1.7 μm) by gradient elution using 0.2% concentration of aqueous ammonia solution and acetonitrile (containing 0.2% ammonia) as mobile phases. Qualification was achieved by negative electrospray ionization with multiple reaction monitoring (MRM). Meanwhile, the external standard method for quantitation was applied. In accordance with the results, the recoveries of hyaluronic acid in dairy products were 91.4%~106.2%, and RSDs were 2.3%~6.7% at the addition levels of 0.5~200 mg/kg. Presumably, with a limit of quantification of 0.5 mg/kg, the demonstrated method was easy and feasible for quantifying the hyaluronic acid in dairy products. This method was used to detect commercially dairy products, and the tested samples meet the standard requirements. |
| abstractGer |
The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for determining hyaluronic acid in dairy products was proposed in this study. The samples were degraded by hyaluronidase, diluted with acetonitrile, and then purified by PRIME HLB cartridge. The analysis was conducted on a BEH Amide column (2.1 mm×100 mm, 1.7 μm) by gradient elution using 0.2% concentration of aqueous ammonia solution and acetonitrile (containing 0.2% ammonia) as mobile phases. Qualification was achieved by negative electrospray ionization with multiple reaction monitoring (MRM). Meanwhile, the external standard method for quantitation was applied. In accordance with the results, the recoveries of hyaluronic acid in dairy products were 91.4%~106.2%, and RSDs were 2.3%~6.7% at the addition levels of 0.5~200 mg/kg. Presumably, with a limit of quantification of 0.5 mg/kg, the demonstrated method was easy and feasible for quantifying the hyaluronic acid in dairy products. This method was used to detect commercially dairy products, and the tested samples meet the standard requirements. |
| abstract_unstemmed |
The approach combining ultra-high performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for determining hyaluronic acid in dairy products was proposed in this study. The samples were degraded by hyaluronidase, diluted with acetonitrile, and then purified by PRIME HLB cartridge. The analysis was conducted on a BEH Amide column (2.1 mm×100 mm, 1.7 μm) by gradient elution using 0.2% concentration of aqueous ammonia solution and acetonitrile (containing 0.2% ammonia) as mobile phases. Qualification was achieved by negative electrospray ionization with multiple reaction monitoring (MRM). Meanwhile, the external standard method for quantitation was applied. In accordance with the results, the recoveries of hyaluronic acid in dairy products were 91.4%~106.2%, and RSDs were 2.3%~6.7% at the addition levels of 0.5~200 mg/kg. Presumably, with a limit of quantification of 0.5 mg/kg, the demonstrated method was easy and feasible for quantifying the hyaluronic acid in dairy products. This method was used to detect commercially dairy products, and the tested samples meet the standard requirements. |
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Determination of Hyaluronic Acid in Dairy Products by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
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https://doi.org/10.13386/j.issn1002-0306.2022070057 https://doaj.org/article/8b478fa0307a4c3d835234f1382f741e http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070057 https://doaj.org/toc/1002-0306 |
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Weijiang FAN Yujuan CHEN Wenwen CHEN Liping QIAO Cuiling WANG |
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