Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin

Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B Ausführliche Beschreibung

Gespeichert in:

Autor*in:	da Silva CN [verfasserIn] Miot HA [verfasserIn] Grassi TF [verfasserIn] Dias-Melício LA [verfasserIn] Santos L [verfasserIn] Espósito ACC [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	melasma immunofluorescence technique endothelin-1 endothelin receptor-a endothelin receptor-b

Übergeordnetes Werk:	In: Clinical, Cosmetic and Investigational Dermatology - Dove Medical Press, 2009, (2023), Seite 2847-2853
Übergeordnetes Werk:	year:2023 ; pages:2847-2853

Links:	Link aufrufen Link aufrufen Journal toc

Katalog-ID:	DOAJ090934458

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allfields	(DE-627)DOAJ090934458 (DE-599)DOAJef9f167dc9ea46b58e0752a9dce42638 DE-627 ger DE-627 rakwb eng RL1-803 da Silva CN verfasserin aut Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B melasma immunofluorescence technique endothelin-1 endothelin receptor-a endothelin receptor-b Dermatology Miot HA verfasserin aut Grassi TF verfasserin aut Dias-Melício LA verfasserin aut Santos L verfasserin aut Espósito ACC verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2023), Seite 2847-2853 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2023 pages:2847-2853 https://doaj.org/article/ef9f167dc9ea46b58e0752a9dce42638 kostenfrei https://www.dovepress.com/expression-of-endothelin-1-endothelin-receptor-a-and-endothelin-recept-peer-reviewed-fulltext-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2023 2847-2853
spelling	(DE-627)DOAJ090934458 (DE-599)DOAJef9f167dc9ea46b58e0752a9dce42638 DE-627 ger DE-627 rakwb eng RL1-803 da Silva CN verfasserin aut Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B melasma immunofluorescence technique endothelin-1 endothelin receptor-a endothelin receptor-b Dermatology Miot HA verfasserin aut Grassi TF verfasserin aut Dias-Melício LA verfasserin aut Santos L verfasserin aut Espósito ACC verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2023), Seite 2847-2853 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2023 pages:2847-2853 https://doaj.org/article/ef9f167dc9ea46b58e0752a9dce42638 kostenfrei https://www.dovepress.com/expression-of-endothelin-1-endothelin-receptor-a-and-endothelin-recept-peer-reviewed-fulltext-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2023 2847-2853
allfields_unstemmed	(DE-627)DOAJ090934458 (DE-599)DOAJef9f167dc9ea46b58e0752a9dce42638 DE-627 ger DE-627 rakwb eng RL1-803 da Silva CN verfasserin aut Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B melasma immunofluorescence technique endothelin-1 endothelin receptor-a endothelin receptor-b Dermatology Miot HA verfasserin aut Grassi TF verfasserin aut Dias-Melício LA verfasserin aut Santos L verfasserin aut Espósito ACC verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2023), Seite 2847-2853 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2023 pages:2847-2853 https://doaj.org/article/ef9f167dc9ea46b58e0752a9dce42638 kostenfrei https://www.dovepress.com/expression-of-endothelin-1-endothelin-receptor-a-and-endothelin-recept-peer-reviewed-fulltext-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2023 2847-2853
allfieldsGer	(DE-627)DOAJ090934458 (DE-599)DOAJef9f167dc9ea46b58e0752a9dce42638 DE-627 ger DE-627 rakwb eng RL1-803 da Silva CN verfasserin aut Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B melasma immunofluorescence technique endothelin-1 endothelin receptor-a endothelin receptor-b Dermatology Miot HA verfasserin aut Grassi TF verfasserin aut Dias-Melício LA verfasserin aut Santos L verfasserin aut Espósito ACC verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2023), Seite 2847-2853 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2023 pages:2847-2853 https://doaj.org/article/ef9f167dc9ea46b58e0752a9dce42638 kostenfrei https://www.dovepress.com/expression-of-endothelin-1-endothelin-receptor-a-and-endothelin-recept-peer-reviewed-fulltext-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2023 2847-2853
allfieldsSound	(DE-627)DOAJ090934458 (DE-599)DOAJef9f167dc9ea46b58e0752a9dce42638 DE-627 ger DE-627 rakwb eng RL1-803 da Silva CN verfasserin aut Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B melasma immunofluorescence technique endothelin-1 endothelin receptor-a endothelin receptor-b Dermatology Miot HA verfasserin aut Grassi TF verfasserin aut Dias-Melício LA verfasserin aut Santos L verfasserin aut Espósito ACC verfasserin aut In Clinical, Cosmetic and Investigational Dermatology Dove Medical Press, 2009 (2023), Seite 2847-2853 (DE-627)600305953 (DE-600)2494852-4 11787015 nnns year:2023 pages:2847-2853 https://doaj.org/article/ef9f167dc9ea46b58e0752a9dce42638 kostenfrei https://www.dovepress.com/expression-of-endothelin-1-endothelin-receptor-a-and-endothelin-recept-peer-reviewed-fulltext-article-CCID kostenfrei https://doaj.org/toc/1178-7015 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2023 2847-2853
language	English
source	In Clinical, Cosmetic and Investigational Dermatology (2023), Seite 2847-2853 year:2023 pages:2847-2853
sourceStr	In Clinical, Cosmetic and Investigational Dermatology (2023), Seite 2847-2853 year:2023 pages:2847-2853
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	melasma immunofluorescence technique endothelin-1 endothelin receptor-a endothelin receptor-b Dermatology
isfreeaccess_bool	true
container_title	Clinical, Cosmetic and Investigational Dermatology
authorswithroles_txt_mv	da Silva CN @@aut@@ Miot HA @@aut@@ Grassi TF @@aut@@ Dias-Melício LA @@aut@@ Santos L @@aut@@ Espósito ACC @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	600305953
id	DOAJ090934458
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">DOAJ090934458</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412230041.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">240412s2023 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ090934458</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJef9f167dc9ea46b58e0752a9dce42638</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RL1-803</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">da Silva CN</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. 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spellingShingle	da Silva CN misc RL1-803 misc melasma misc immunofluorescence technique misc endothelin-1 misc endothelin receptor-a misc endothelin receptor-b misc Dermatology Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin
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topic_title	RL1-803 Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin melasma immunofluorescence technique endothelin-1 endothelin receptor-a endothelin receptor-b
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title	Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin
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title_full	Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin
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title_sort	expression of endothelin-1, endothelin receptor-a, and endothelin receptor-b in facial melasma compared to adjacent skin
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title_auth	Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin
abstract	Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B
abstractGer	Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B
abstract_unstemmed	Carolina Nunhez da Silva,1 Hélio Amante Miot,1 Tony Fernando Grassi,2 Luciane Alarcão Dias-Melício,2– 4 Leandro Santos,2,3 Ana Cláudia Cavalcante Espósito1 1Department of Dermatology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 2UNIPEX - Experimental Research Unit, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 3Laboratory of Immunopathology and Infectious Agents – LIAI, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil; 4Department of Pathology, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, BrazilCorrespondence: Ana Cláudia Cavalcante Espósito, Division of Dermatology and Radiotherapy, São Paulo State University (UNESP) - Medical School of Botucatu, Botucatu, São Paulo State, Brazil, Email anaclaudiaespositogmail.comBackground/Objectives: Although melasma is highly prevalent, its pathogenesis is not yet fully understood. In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B
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title_short	Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin
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In the skin, endothelin-1 (ET-1) is primarily produced by keratinocytes in response to UVB exposure, which is mediated by an increase in IL-1α or reactive oxygen species. ET-1 plays a role in melanogenesis by binding to specific receptor B (ERB) or receptor A (ERA). However, the expression of ET-1, ERA, and ERB in melasma has not been systematically investigated. The objective of this study was to evaluate the expression of ET-1, ERA, and ERB in facial melasma compared to the adjacent unaffected skin.Methods: Cross-sectional study, with 40 skin samples (20: facial melasma; 20: adjacent unaffected skin) from women with facial melasma without treatment for 30 days except for sunscreen. A triple staining immunofluorescence technique was performed for anti-vimentin, DAPI, plus one of the following antibodies: (a) anti-ET1, (b) anti-ERA; (c) anti-ERB. Interfollicular areas on the slides of each topography (melasma; unaffected skin) were photographed in triplicate under confocal laser microscopy. The mean staining intensities of the image histograms (0– 255 pixels intensity) were estimated for different types of cells (suprabasal keratinocytes, basal layer, and upper dermis) and were blindly compared between topographies.Results: The mean (SD) age of the participants was 44.9 (9.2). The expression of ET-1 was increased in the whole epidermis with melasma when compared to the adjacent skin, being 32.8% (CI95% 14.7%– 52.6%) higher in the spinous layer (p=0.013), 30.4% (CI95% 13.7%– 47.9%) higher in the basal layer (p=0.014), and 29.7% (CI95% 11.4%– 49.7%) higher in the melanocytes (p=0.006). There was no noticeable expression of ET-1 within the cells on the upper dermis. Neither ERA nor ERB resulted in differential epidermal expression between melasma and unaffected skin (p≥ 0.1).Conclusion: ET-1 is expressed more intensely on the epidermis from the skin with facial melasma compared to the unaffected adjacent skin.Keywords: melasma, immunofluorescence technique, endothelin-1, endothelin receptor-A, endothelin receptor-B</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">melasma</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">immunofluorescence technique</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">endothelin-1</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">endothelin receptor-a</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">endothelin receptor-b</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Dermatology</subfield></datafield><datafield tag="700" ind1="0" ind2=" 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2009</subfield><subfield code="g">(2023), Seite 2847-2853</subfield><subfield code="w">(DE-627)600305953</subfield><subfield code="w">(DE-600)2494852-4</subfield><subfield code="x">11787015</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">year:2023</subfield><subfield code="g">pages:2847-2853</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/ef9f167dc9ea46b58e0752a9dce42638</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.dovepress.com/expression-of-endothelin-1-endothelin-receptor-a-and-endothelin-recept-peer-reviewed-fulltext-article-CCID</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1178-7015</subfield><subfield code="y">Journal toc</subfield><subfield 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Expression of Endothelin-1, Endothelin Receptor-A, and Endothelin Receptor-B in facial melasma compared to adjacent skin

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