Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora

Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Ruiyun Yu [verfasserIn] Qiang Gao [verfasserIn] Chao Zhang [verfasserIn] Haihuan Yang [verfasserIn] Xingwei Yao [verfasserIn] Yikun Sun [verfasserIn] Hongming Ma [verfasserIn] Han-Hwa Hu [verfasserIn] Dayong Ma [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Xinglou Chengqi decoction Acute ischemic stroke Phlegm-heat and fu-organ excess pattern Brain-gut axis Immune inflammation

Übergeordnetes Werk:	In: Journal of Traditional Chinese Medical Sciences - Elsevier, 2015, 10(2023), 4, Seite 502-511
Übergeordnetes Werk:	volume:10 ; year:2023 ; number:4 ; pages:502-511

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.jtcms.2023.09.011

Katalog-ID:	DOAJ091002966

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ091002966
003	DE-627
005	20240414085559.0
007	cr uuu---uuuuu
008	240412s2023 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1016/j.jtcms.2023.09.011 \|2 doi
035			\|a (DE-627)DOAJ091002966
035			\|a (DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a RZ409.7-999
100	0		\|a Ruiyun Yu \|e verfasserin \|4 aut
245	1	0	\|a Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora
264		1	\|c 2023
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably.
650		4	\|a Xinglou Chengqi decoction
650		4	\|a Acute ischemic stroke
650		4	\|a Phlegm-heat and fu-organ excess pattern
650		4	\|a Brain-gut axis
650		4	\|a Immune inflammation
653		0	\|a Miscellaneous systems and treatments
700	0		\|a Qiang Gao \|e verfasserin \|4 aut
700	0		\|a Chao Zhang \|e verfasserin \|4 aut
700	0		\|a Haihuan Yang \|e verfasserin \|4 aut
700	0		\|a Xingwei Yao \|e verfasserin \|4 aut
700	0		\|a Yikun Sun \|e verfasserin \|4 aut
700	0		\|a Hongming Ma \|e verfasserin \|4 aut
700	0		\|a Han-Hwa Hu \|e verfasserin \|4 aut
700	0		\|a Dayong Ma \|e verfasserin \|4 aut
773	0	8	\|i In \|t Journal of Traditional Chinese Medical Sciences \|d Elsevier, 2015 \|g 10(2023), 4, Seite 502-511 \|w (DE-627)835584712 \|w (DE-600)2834228-8 \|x 25890395 \|7 nnns
773	1	8	\|g volume:10 \|g year:2023 \|g number:4 \|g pages:502-511
856	4	0	\|u https://doi.org/10.1016/j.jtcms.2023.09.011 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe \|z kostenfrei
856	4	0	\|u http://www.sciencedirect.com/science/article/pii/S2095754823000601 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/2095-7548 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 10 \|j 2023 \|e 4 \|h 502-511

Indexfelder

author_variant	r y ry q g qg c z cz h y hy x y xy y s ys h m hm h h h hhh d m dm
matchkey_str	article:25890395:2023----::fetoxnluhnqdccinnetaadeihrlmuenlmainnieihia
hierarchy_sort_str	2023
callnumber-subject-code	RZ
publishDate	2023
allfields	10.1016/j.jtcms.2023.09.011 doi (DE-627)DOAJ091002966 (DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe DE-627 ger DE-627 rakwb eng RZ409.7-999 Ruiyun Yu verfasserin aut Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably. Xinglou Chengqi decoction Acute ischemic stroke Phlegm-heat and fu-organ excess pattern Brain-gut axis Immune inflammation Miscellaneous systems and treatments Qiang Gao verfasserin aut Chao Zhang verfasserin aut Haihuan Yang verfasserin aut Xingwei Yao verfasserin aut Yikun Sun verfasserin aut Hongming Ma verfasserin aut Han-Hwa Hu verfasserin aut Dayong Ma verfasserin aut In Journal of Traditional Chinese Medical Sciences Elsevier, 2015 10(2023), 4, Seite 502-511 (DE-627)835584712 (DE-600)2834228-8 25890395 nnns volume:10 year:2023 number:4 pages:502-511 https://doi.org/10.1016/j.jtcms.2023.09.011 kostenfrei https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe kostenfrei http://www.sciencedirect.com/science/article/pii/S2095754823000601 kostenfrei https://doaj.org/toc/2095-7548 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023 4 502-511
spelling	10.1016/j.jtcms.2023.09.011 doi (DE-627)DOAJ091002966 (DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe DE-627 ger DE-627 rakwb eng RZ409.7-999 Ruiyun Yu verfasserin aut Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably. Xinglou Chengqi decoction Acute ischemic stroke Phlegm-heat and fu-organ excess pattern Brain-gut axis Immune inflammation Miscellaneous systems and treatments Qiang Gao verfasserin aut Chao Zhang verfasserin aut Haihuan Yang verfasserin aut Xingwei Yao verfasserin aut Yikun Sun verfasserin aut Hongming Ma verfasserin aut Han-Hwa Hu verfasserin aut Dayong Ma verfasserin aut In Journal of Traditional Chinese Medical Sciences Elsevier, 2015 10(2023), 4, Seite 502-511 (DE-627)835584712 (DE-600)2834228-8 25890395 nnns volume:10 year:2023 number:4 pages:502-511 https://doi.org/10.1016/j.jtcms.2023.09.011 kostenfrei https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe kostenfrei http://www.sciencedirect.com/science/article/pii/S2095754823000601 kostenfrei https://doaj.org/toc/2095-7548 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023 4 502-511
allfields_unstemmed	10.1016/j.jtcms.2023.09.011 doi (DE-627)DOAJ091002966 (DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe DE-627 ger DE-627 rakwb eng RZ409.7-999 Ruiyun Yu verfasserin aut Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably. Xinglou Chengqi decoction Acute ischemic stroke Phlegm-heat and fu-organ excess pattern Brain-gut axis Immune inflammation Miscellaneous systems and treatments Qiang Gao verfasserin aut Chao Zhang verfasserin aut Haihuan Yang verfasserin aut Xingwei Yao verfasserin aut Yikun Sun verfasserin aut Hongming Ma verfasserin aut Han-Hwa Hu verfasserin aut Dayong Ma verfasserin aut In Journal of Traditional Chinese Medical Sciences Elsevier, 2015 10(2023), 4, Seite 502-511 (DE-627)835584712 (DE-600)2834228-8 25890395 nnns volume:10 year:2023 number:4 pages:502-511 https://doi.org/10.1016/j.jtcms.2023.09.011 kostenfrei https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe kostenfrei http://www.sciencedirect.com/science/article/pii/S2095754823000601 kostenfrei https://doaj.org/toc/2095-7548 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023 4 502-511
allfieldsGer	10.1016/j.jtcms.2023.09.011 doi (DE-627)DOAJ091002966 (DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe DE-627 ger DE-627 rakwb eng RZ409.7-999 Ruiyun Yu verfasserin aut Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably. Xinglou Chengqi decoction Acute ischemic stroke Phlegm-heat and fu-organ excess pattern Brain-gut axis Immune inflammation Miscellaneous systems and treatments Qiang Gao verfasserin aut Chao Zhang verfasserin aut Haihuan Yang verfasserin aut Xingwei Yao verfasserin aut Yikun Sun verfasserin aut Hongming Ma verfasserin aut Han-Hwa Hu verfasserin aut Dayong Ma verfasserin aut In Journal of Traditional Chinese Medical Sciences Elsevier, 2015 10(2023), 4, Seite 502-511 (DE-627)835584712 (DE-600)2834228-8 25890395 nnns volume:10 year:2023 number:4 pages:502-511 https://doi.org/10.1016/j.jtcms.2023.09.011 kostenfrei https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe kostenfrei http://www.sciencedirect.com/science/article/pii/S2095754823000601 kostenfrei https://doaj.org/toc/2095-7548 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023 4 502-511
allfieldsSound	10.1016/j.jtcms.2023.09.011 doi (DE-627)DOAJ091002966 (DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe DE-627 ger DE-627 rakwb eng RZ409.7-999 Ruiyun Yu verfasserin aut Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably. Xinglou Chengqi decoction Acute ischemic stroke Phlegm-heat and fu-organ excess pattern Brain-gut axis Immune inflammation Miscellaneous systems and treatments Qiang Gao verfasserin aut Chao Zhang verfasserin aut Haihuan Yang verfasserin aut Xingwei Yao verfasserin aut Yikun Sun verfasserin aut Hongming Ma verfasserin aut Han-Hwa Hu verfasserin aut Dayong Ma verfasserin aut In Journal of Traditional Chinese Medical Sciences Elsevier, 2015 10(2023), 4, Seite 502-511 (DE-627)835584712 (DE-600)2834228-8 25890395 nnns volume:10 year:2023 number:4 pages:502-511 https://doi.org/10.1016/j.jtcms.2023.09.011 kostenfrei https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe kostenfrei http://www.sciencedirect.com/science/article/pii/S2095754823000601 kostenfrei https://doaj.org/toc/2095-7548 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2023 4 502-511
language	English
source	In Journal of Traditional Chinese Medical Sciences 10(2023), 4, Seite 502-511 volume:10 year:2023 number:4 pages:502-511
sourceStr	In Journal of Traditional Chinese Medical Sciences 10(2023), 4, Seite 502-511 volume:10 year:2023 number:4 pages:502-511
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Xinglou Chengqi decoction Acute ischemic stroke Phlegm-heat and fu-organ excess pattern Brain-gut axis Immune inflammation Miscellaneous systems and treatments
isfreeaccess_bool	true
container_title	Journal of Traditional Chinese Medical Sciences
authorswithroles_txt_mv	Ruiyun Yu @@aut@@ Qiang Gao @@aut@@ Chao Zhang @@aut@@ Haihuan Yang @@aut@@ Xingwei Yao @@aut@@ Yikun Sun @@aut@@ Hongming Ma @@aut@@ Han-Hwa Hu @@aut@@ Dayong Ma @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	835584712
id	DOAJ091002966
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ091002966</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414085559.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">240412s2023 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.jtcms.2023.09.011</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ091002966</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RZ409.7-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Ruiyun Yu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Xinglou Chengqi decoction</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Acute ischemic stroke</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Phlegm-heat and fu-organ excess pattern</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Brain-gut axis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immune inflammation</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Miscellaneous systems and treatments</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Qiang Gao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chao Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Haihuan Yang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xingwei Yao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yikun Sun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hongming Ma</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Han-Hwa Hu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Dayong Ma</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of Traditional Chinese Medical Sciences</subfield><subfield code="d">Elsevier, 2015</subfield><subfield code="g">10(2023), 4, Seite 502-511</subfield><subfield code="w">(DE-627)835584712</subfield><subfield code="w">(DE-600)2834228-8</subfield><subfield code="x">25890395</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:10</subfield><subfield code="g">year:2023</subfield><subfield code="g">number:4</subfield><subfield code="g">pages:502-511</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.jtcms.2023.09.011</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.sciencedirect.com/science/article/pii/S2095754823000601</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2095-7548</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">10</subfield><subfield code="j">2023</subfield><subfield code="e">4</subfield><subfield code="h">502-511</subfield></datafield></record></collection>
callnumber-first	R - Medicine
author	Ruiyun Yu
spellingShingle	Ruiyun Yu misc RZ409.7-999 misc Xinglou Chengqi decoction misc Acute ischemic stroke misc Phlegm-heat and fu-organ excess pattern misc Brain-gut axis misc Immune inflammation misc Miscellaneous systems and treatments Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora
authorStr	Ruiyun Yu
ppnlink_with_tag_str_mv	@@773@@(DE-627)835584712
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	RZ409
illustrated	Not Illustrated
issn	25890395
topic_title	RZ409.7-999 Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora Xinglou Chengqi decoction Acute ischemic stroke Phlegm-heat and fu-organ excess pattern Brain-gut axis Immune inflammation
topic	misc RZ409.7-999 misc Xinglou Chengqi decoction misc Acute ischemic stroke misc Phlegm-heat and fu-organ excess pattern misc Brain-gut axis misc Immune inflammation misc Miscellaneous systems and treatments
topic_unstemmed	misc RZ409.7-999 misc Xinglou Chengqi decoction misc Acute ischemic stroke misc Phlegm-heat and fu-organ excess pattern misc Brain-gut axis misc Immune inflammation misc Miscellaneous systems and treatments
topic_browse	misc RZ409.7-999 misc Xinglou Chengqi decoction misc Acute ischemic stroke misc Phlegm-heat and fu-organ excess pattern misc Brain-gut axis misc Immune inflammation misc Miscellaneous systems and treatments
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Journal of Traditional Chinese Medical Sciences
hierarchy_parent_id	835584712
hierarchy_top_title	Journal of Traditional Chinese Medical Sciences
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)835584712 (DE-600)2834228-8
title	Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora
ctrlnum	(DE-627)DOAJ091002966 (DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe
title_full	Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora
author_sort	Ruiyun Yu
journal	Journal of Traditional Chinese Medical Sciences
journalStr	Journal of Traditional Chinese Medical Sciences
callnumber-first-code	R
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2023
contenttype_str_mv	txt
container_start_page	502
author_browse	Ruiyun Yu Qiang Gao Chao Zhang Haihuan Yang Xingwei Yao Yikun Sun Hongming Ma Han-Hwa Hu Dayong Ma
container_volume	10
class	RZ409.7-999
format_se	Elektronische Aufsätze
author-letter	Ruiyun Yu
doi_str_mv	10.1016/j.jtcms.2023.09.011
author2-role	verfasserin
title_sort	effects of xinglou chengqi decoction on central and peripheral immune inflammation in mice with ais are correlated with intestinal flora
callnumber	RZ409.7-999
title_auth	Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora
abstract	Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably.
abstractGer	Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably.
abstract_unstemmed	Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	4
title_short	Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora
url	https://doi.org/10.1016/j.jtcms.2023.09.011 https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe http://www.sciencedirect.com/science/article/pii/S2095754823000601 https://doaj.org/toc/2095-7548
remote_bool	true
author2	Qiang Gao Chao Zhang Haihuan Yang Xingwei Yao Yikun Sun Hongming Ma Han-Hwa Hu Dayong Ma
author2Str	Qiang Gao Chao Zhang Haihuan Yang Xingwei Yao Yikun Sun Hongming Ma Han-Hwa Hu Dayong Ma
ppnlink	835584712
callnumber-subject	RZ - Other Systems of Medicine
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.1016/j.jtcms.2023.09.011
callnumber-a	RZ409.7-999
up_date	2024-07-03T17:51:29.916Z
_version_	1803581222991953920
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ091002966</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414085559.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">240412s2023 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.jtcms.2023.09.011</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ091002966</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ8c694f2a26ee486884d8b8697f8cbafe</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RZ409.7-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Ruiyun Yu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objective: To explore the effect of Xinglou Chengqi decoction (XCD) on the brain-gut axis in mice with acute ischemic stroke. Methods: A cerebral ischemia-reperfusion model identified as phlegm-heat and fu-organ excess pattern was established by reforming the Longa method and based on the diagnostic criteria of traditional Chinese medicine. Sixty specific-pathogen free male C57BL/6 mice were randomly divided into five groups, and 36 antibiotic-treated (ABX) mice were randomly divided into three groups. Cecum norepinephrine (NE) and motilin (MTL) were detected by enzyme-linked immunosorbent assay, cecum tyrosine hydroxylase (TH) protein expression level was detected by immunohistochemistry, Iba-1 and glial fibrillary acidic protein (GFAP) expression levels were detected by immunofluorescence, and serum inflammatory factor contents were detected using the flow multifactor technique. Results: Compared to the sham group, a significant increase in TH expression level was observed in the cecum in the middle cerebral artery occlusion (MCAO) group (P = .015). MTL levels were significantly increased in the MCAO group compared with the sham group (P < .001) and significantly decreased in the XCD and nimodipine (Nim) groups compared with the MCAO group (P = .003 and P = .007, respectively). Astrocytes and microglia were markedly activated in the MCAO group, whereas activation was decreased in the XCD group compared to the MCAO group. Antibiotic-treated (ABX) mice astrocytes and microglia were obviously activated in the ABX-MCAO group, and activation tended to be lower in the ABX-XCD group. Interleukin-22 level significantly increased in the ABX-XCD group compared with the ABX-MCAO (P = .021). Conclusion: XCD has a significant regulatory effect on the immune pathway of the brain-gut axis in mice with acute ischemic stroke, while the removal of the gut microbiota attenuates the effect considerably.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Xinglou Chengqi decoction</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Acute ischemic stroke</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Phlegm-heat and fu-organ excess pattern</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Brain-gut axis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immune inflammation</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Miscellaneous systems and treatments</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Qiang Gao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chao Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Haihuan Yang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xingwei Yao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yikun Sun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hongming Ma</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Han-Hwa Hu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Dayong Ma</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of Traditional Chinese Medical Sciences</subfield><subfield code="d">Elsevier, 2015</subfield><subfield code="g">10(2023), 4, Seite 502-511</subfield><subfield code="w">(DE-627)835584712</subfield><subfield code="w">(DE-600)2834228-8</subfield><subfield code="x">25890395</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:10</subfield><subfield code="g">year:2023</subfield><subfield code="g">number:4</subfield><subfield code="g">pages:502-511</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.jtcms.2023.09.011</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/8c694f2a26ee486884d8b8697f8cbafe</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.sciencedirect.com/science/article/pii/S2095754823000601</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2095-7548</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">10</subfield><subfield code="j">2023</subfield><subfield code="e">4</subfield><subfield code="h">502-511</subfield></datafield></record></collection>
score	7.3991594

Nicht das Richtige dabei?

Schreiben Sie uns!

Effects of Xinglou Chengqi decoction on central and peripheral immune inflammation in mice with AIS are correlated with intestinal flora

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?