Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy

Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs. We show a method based on the use of Light sheet fluorescence microscopy (LSFM) which allows the...
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Autor*in:	Tortora Giorgia [verfasserIn] Buratti Stefano [verfasserIn] Grenzi Matteo [verfasserIn] Costa Alex [verfasserIn] Bassi Andrea [verfasserIn] Candeo Alessia [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Übergeordnetes Werk:	In: EPJ Web of Conferences - EDP Sciences, 2010, 287, p 03012(2023)
Übergeordnetes Werk:	volume:287, p 03012 ; year:2023

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1051/epjconf/202328703012

Katalog-ID:	DOAJ091055032

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callnumber-first	Q - Science
author	Tortora Giorgia
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topic_title	QC1-999 Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy
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title	Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy
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title_full	Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy
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title_sort	imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy
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title_auth	Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy
abstract	Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs. We show a method based on the use of Light sheet fluorescence microscopy (LSFM) which allows the quasi-physiological analysis of Arabidopsis thaliana plant roots hairs with excellent spatial and temporal resolution over a wide field of view. We show how the healthy growing root hairs are linked to precise oscillations and how a disruption of this mechanism can be associated to specific genes.
abstractGer	Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs. We show a method based on the use of Light sheet fluorescence microscopy (LSFM) which allows the quasi-physiological analysis of Arabidopsis thaliana plant roots hairs with excellent spatial and temporal resolution over a wide field of view. We show how the healthy growing root hairs are linked to precise oscillations and how a disruption of this mechanism can be associated to specific genes.
abstract_unstemmed	Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs. We show a method based on the use of Light sheet fluorescence microscopy (LSFM) which allows the quasi-physiological analysis of Arabidopsis thaliana plant roots hairs with excellent spatial and temporal resolution over a wide field of view. We show how the healthy growing root hairs are linked to precise oscillations and how a disruption of this mechanism can be associated to specific genes.
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title_short	Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy
url	https://doi.org/10.1051/epjconf/202328703012 https://doaj.org/article/b84e1c2449bc4e8fb6bb6fe15515dddf https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_03012.pdf https://doaj.org/toc/2100-014X
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Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy

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