Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection

Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enric...
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Autor*in:	Eunyoung Jeon [verfasserIn] Bonhan Koo [verfasserIn] Suyeon Kim [verfasserIn] Jieun Kim [verfasserIn] Yeonuk Yu [verfasserIn] Hyowon Jang [verfasserIn] Minju Lee [verfasserIn] Sung-Han Kim [verfasserIn] Taejoon Kang [verfasserIn] Sang Kyung Kim [verfasserIn] Rhokyun Kwak [verfasserIn] Yong Shin [verfasserIn] Joonseok Lee [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Übergeordnetes Werk:	In: Nature Communications - Nature Portfolio, 2016, 15(2024), 1, Seite 13
Übergeordnetes Werk:	volume:15 ; year:2024 ; number:1 ; pages:13

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1038/s41467-024-45467-w

Katalog-ID:	DOAJ091184754

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allfields	10.1038/s41467-024-45467-w doi (DE-627)DOAJ091184754 (DE-599)DOAJ26e198cbc7e34cac9e7700a23e910584 DE-627 ger DE-627 rakwb eng Eunyoung Jeon verfasserin aut Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids. Science Q Bonhan Koo verfasserin aut Suyeon Kim verfasserin aut Jieun Kim verfasserin aut Yeonuk Yu verfasserin aut Hyowon Jang verfasserin aut Minju Lee verfasserin aut Sung-Han Kim verfasserin aut Taejoon Kang verfasserin aut Sang Kyung Kim verfasserin aut Rhokyun Kwak verfasserin aut Yong Shin verfasserin aut Joonseok Lee verfasserin aut In Nature Communications Nature Portfolio, 2016 15(2024), 1, Seite 13 (DE-627)626457688 (DE-600)2553671-0 20411723 nnns volume:15 year:2024 number:1 pages:13 https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/article/26e198cbc7e34cac9e7700a23e910584 kostenfrei https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/toc/2041-1723 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_211 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2110 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 13
spelling	10.1038/s41467-024-45467-w doi (DE-627)DOAJ091184754 (DE-599)DOAJ26e198cbc7e34cac9e7700a23e910584 DE-627 ger DE-627 rakwb eng Eunyoung Jeon verfasserin aut Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids. Science Q Bonhan Koo verfasserin aut Suyeon Kim verfasserin aut Jieun Kim verfasserin aut Yeonuk Yu verfasserin aut Hyowon Jang verfasserin aut Minju Lee verfasserin aut Sung-Han Kim verfasserin aut Taejoon Kang verfasserin aut Sang Kyung Kim verfasserin aut Rhokyun Kwak verfasserin aut Yong Shin verfasserin aut Joonseok Lee verfasserin aut In Nature Communications Nature Portfolio, 2016 15(2024), 1, Seite 13 (DE-627)626457688 (DE-600)2553671-0 20411723 nnns volume:15 year:2024 number:1 pages:13 https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/article/26e198cbc7e34cac9e7700a23e910584 kostenfrei https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/toc/2041-1723 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_211 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2110 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 13
allfields_unstemmed	10.1038/s41467-024-45467-w doi (DE-627)DOAJ091184754 (DE-599)DOAJ26e198cbc7e34cac9e7700a23e910584 DE-627 ger DE-627 rakwb eng Eunyoung Jeon verfasserin aut Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids. Science Q Bonhan Koo verfasserin aut Suyeon Kim verfasserin aut Jieun Kim verfasserin aut Yeonuk Yu verfasserin aut Hyowon Jang verfasserin aut Minju Lee verfasserin aut Sung-Han Kim verfasserin aut Taejoon Kang verfasserin aut Sang Kyung Kim verfasserin aut Rhokyun Kwak verfasserin aut Yong Shin verfasserin aut Joonseok Lee verfasserin aut In Nature Communications Nature Portfolio, 2016 15(2024), 1, Seite 13 (DE-627)626457688 (DE-600)2553671-0 20411723 nnns volume:15 year:2024 number:1 pages:13 https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/article/26e198cbc7e34cac9e7700a23e910584 kostenfrei https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/toc/2041-1723 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_211 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2110 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 13
allfieldsGer	10.1038/s41467-024-45467-w doi (DE-627)DOAJ091184754 (DE-599)DOAJ26e198cbc7e34cac9e7700a23e910584 DE-627 ger DE-627 rakwb eng Eunyoung Jeon verfasserin aut Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids. Science Q Bonhan Koo verfasserin aut Suyeon Kim verfasserin aut Jieun Kim verfasserin aut Yeonuk Yu verfasserin aut Hyowon Jang verfasserin aut Minju Lee verfasserin aut Sung-Han Kim verfasserin aut Taejoon Kang verfasserin aut Sang Kyung Kim verfasserin aut Rhokyun Kwak verfasserin aut Yong Shin verfasserin aut Joonseok Lee verfasserin aut In Nature Communications Nature Portfolio, 2016 15(2024), 1, Seite 13 (DE-627)626457688 (DE-600)2553671-0 20411723 nnns volume:15 year:2024 number:1 pages:13 https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/article/26e198cbc7e34cac9e7700a23e910584 kostenfrei https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/toc/2041-1723 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_211 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2110 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 13
allfieldsSound	10.1038/s41467-024-45467-w doi (DE-627)DOAJ091184754 (DE-599)DOAJ26e198cbc7e34cac9e7700a23e910584 DE-627 ger DE-627 rakwb eng Eunyoung Jeon verfasserin aut Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids. Science Q Bonhan Koo verfasserin aut Suyeon Kim verfasserin aut Jieun Kim verfasserin aut Yeonuk Yu verfasserin aut Hyowon Jang verfasserin aut Minju Lee verfasserin aut Sung-Han Kim verfasserin aut Taejoon Kang verfasserin aut Sang Kyung Kim verfasserin aut Rhokyun Kwak verfasserin aut Yong Shin verfasserin aut Joonseok Lee verfasserin aut In Nature Communications Nature Portfolio, 2016 15(2024), 1, Seite 13 (DE-627)626457688 (DE-600)2553671-0 20411723 nnns volume:15 year:2024 number:1 pages:13 https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/article/26e198cbc7e34cac9e7700a23e910584 kostenfrei https://doi.org/10.1038/s41467-024-45467-w kostenfrei https://doaj.org/toc/2041-1723 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_211 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2110 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 13
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author	Eunyoung Jeon
spellingShingle	Eunyoung Jeon misc Science misc Q Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection
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topic_title	Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection
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title	Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection
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title_full	Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection
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author_browse	Eunyoung Jeon Bonhan Koo Suyeon Kim Jieun Kim Yeonuk Yu Hyowon Jang Minju Lee Sung-Han Kim Taejoon Kang Sang Kyung Kim Rhokyun Kwak Yong Shin Joonseok Lee
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title_sort	biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and pcr-free detection
title_auth	Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection
abstract	Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids.
abstractGer	Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids.
abstract_unstemmed	Abstract Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids.
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title_short	Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection
url	https://doi.org/10.1038/s41467-024-45467-w https://doaj.org/article/26e198cbc7e34cac9e7700a23e910584 https://doaj.org/toc/2041-1723
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author2	Bonhan Koo Suyeon Kim Jieun Kim Yeonuk Yu Hyowon Jang Minju Lee Sung-Han Kim Taejoon Kang Sang Kyung Kim Rhokyun Kwak Yong Shin Joonseok Lee
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