Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i<

<i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Xiaochen Yu [verfasserIn] Jinghui Yu [verfasserIn] Sizhang Liu [verfasserIn] Mingming Liu [verfasserIn] Kangyu Wang [verfasserIn] Mingzhu Zhao [verfasserIn] Yanfang Wang [verfasserIn] Ping Chen [verfasserIn] Jun Lei [verfasserIn] Yi Wang [verfasserIn] Meiping Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	ginsenoside Ro transcriptome glycosyltransferase biosynthesis

Übergeordnetes Werk:	In: Plants - MDPI AG, 2013, 13(2024), 5, p 604
Übergeordnetes Werk:	volume:13 ; year:2024 ; number:5, p 604

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/plants13050604

Katalog-ID:	DOAJ091241650

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allfields	10.3390/plants13050604 doi (DE-627)DOAJ091241650 (DE-599)DOAJ8f06d545accc489a9b37782611f43d42 DE-627 ger DE-627 rakwb eng QK1-989 Xiaochen Yu verfasserin aut Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i< 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes. <i<Panax ginseng</i< ginsenoside Ro transcriptome glycosyltransferase biosynthesis Botany Jinghui Yu verfasserin aut Sizhang Liu verfasserin aut Mingming Liu verfasserin aut Kangyu Wang verfasserin aut Mingzhu Zhao verfasserin aut Yanfang Wang verfasserin aut Ping Chen verfasserin aut Jun Lei verfasserin aut Yi Wang verfasserin aut Meiping Zhang verfasserin aut In Plants MDPI AG, 2013 13(2024), 5, p 604 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:13 year:2024 number:5, p 604 https://doi.org/10.3390/plants13050604 kostenfrei https://doaj.org/article/8f06d545accc489a9b37782611f43d42 kostenfrei https://www.mdpi.com/2223-7747/13/5/604 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2024 5, p 604
spelling	10.3390/plants13050604 doi (DE-627)DOAJ091241650 (DE-599)DOAJ8f06d545accc489a9b37782611f43d42 DE-627 ger DE-627 rakwb eng QK1-989 Xiaochen Yu verfasserin aut Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i< 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes. <i<Panax ginseng</i< ginsenoside Ro transcriptome glycosyltransferase biosynthesis Botany Jinghui Yu verfasserin aut Sizhang Liu verfasserin aut Mingming Liu verfasserin aut Kangyu Wang verfasserin aut Mingzhu Zhao verfasserin aut Yanfang Wang verfasserin aut Ping Chen verfasserin aut Jun Lei verfasserin aut Yi Wang verfasserin aut Meiping Zhang verfasserin aut In Plants MDPI AG, 2013 13(2024), 5, p 604 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:13 year:2024 number:5, p 604 https://doi.org/10.3390/plants13050604 kostenfrei https://doaj.org/article/8f06d545accc489a9b37782611f43d42 kostenfrei https://www.mdpi.com/2223-7747/13/5/604 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2024 5, p 604
allfields_unstemmed	10.3390/plants13050604 doi (DE-627)DOAJ091241650 (DE-599)DOAJ8f06d545accc489a9b37782611f43d42 DE-627 ger DE-627 rakwb eng QK1-989 Xiaochen Yu verfasserin aut Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i< 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes. <i<Panax ginseng</i< ginsenoside Ro transcriptome glycosyltransferase biosynthesis Botany Jinghui Yu verfasserin aut Sizhang Liu verfasserin aut Mingming Liu verfasserin aut Kangyu Wang verfasserin aut Mingzhu Zhao verfasserin aut Yanfang Wang verfasserin aut Ping Chen verfasserin aut Jun Lei verfasserin aut Yi Wang verfasserin aut Meiping Zhang verfasserin aut In Plants MDPI AG, 2013 13(2024), 5, p 604 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:13 year:2024 number:5, p 604 https://doi.org/10.3390/plants13050604 kostenfrei https://doaj.org/article/8f06d545accc489a9b37782611f43d42 kostenfrei https://www.mdpi.com/2223-7747/13/5/604 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2024 5, p 604
allfieldsGer	10.3390/plants13050604 doi (DE-627)DOAJ091241650 (DE-599)DOAJ8f06d545accc489a9b37782611f43d42 DE-627 ger DE-627 rakwb eng QK1-989 Xiaochen Yu verfasserin aut Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i< 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes. <i<Panax ginseng</i< ginsenoside Ro transcriptome glycosyltransferase biosynthesis Botany Jinghui Yu verfasserin aut Sizhang Liu verfasserin aut Mingming Liu verfasserin aut Kangyu Wang verfasserin aut Mingzhu Zhao verfasserin aut Yanfang Wang verfasserin aut Ping Chen verfasserin aut Jun Lei verfasserin aut Yi Wang verfasserin aut Meiping Zhang verfasserin aut In Plants MDPI AG, 2013 13(2024), 5, p 604 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:13 year:2024 number:5, p 604 https://doi.org/10.3390/plants13050604 kostenfrei https://doaj.org/article/8f06d545accc489a9b37782611f43d42 kostenfrei https://www.mdpi.com/2223-7747/13/5/604 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2024 5, p 604
allfieldsSound	10.3390/plants13050604 doi (DE-627)DOAJ091241650 (DE-599)DOAJ8f06d545accc489a9b37782611f43d42 DE-627 ger DE-627 rakwb eng QK1-989 Xiaochen Yu verfasserin aut Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i< 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes. <i<Panax ginseng</i< ginsenoside Ro transcriptome glycosyltransferase biosynthesis Botany Jinghui Yu verfasserin aut Sizhang Liu verfasserin aut Mingming Liu verfasserin aut Kangyu Wang verfasserin aut Mingzhu Zhao verfasserin aut Yanfang Wang verfasserin aut Ping Chen verfasserin aut Jun Lei verfasserin aut Yi Wang verfasserin aut Meiping Zhang verfasserin aut In Plants MDPI AG, 2013 13(2024), 5, p 604 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:13 year:2024 number:5, p 604 https://doi.org/10.3390/plants13050604 kostenfrei https://doaj.org/article/8f06d545accc489a9b37782611f43d42 kostenfrei https://www.mdpi.com/2223-7747/13/5/604 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2024 5, p 604
language	English
source	In Plants 13(2024), 5, p 604 volume:13 year:2024 number:5, p 604
sourceStr	In Plants 13(2024), 5, p 604 volume:13 year:2024 number:5, p 604
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Panax ginseng</i< ginsenoside Ro transcriptome glycosyltransferase biosynthesis Botany
isfreeaccess_bool	true
container_title	Plants
authorswithroles_txt_mv	Xiaochen Yu @@aut@@ Jinghui Yu @@aut@@ Sizhang Liu @@aut@@ Mingming Liu @@aut@@ Kangyu Wang @@aut@@ Mingzhu Zhao @@aut@@ Yanfang Wang @@aut@@ Ping Chen @@aut@@ Jun Lei @@aut@@ Yi Wang @@aut@@ Meiping Zhang @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	737288345
id	DOAJ091241650
language_de	englisch
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callnumber-first	Q - Science
author	Xiaochen Yu
spellingShingle	Xiaochen Yu misc QK1-989 misc <i<Panax ginseng</i< misc ginsenoside Ro misc transcriptome misc glycosyltransferase misc biosynthesis misc Botany Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i<
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topic_title	QK1-989 Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i< <i<Panax ginseng</i< ginsenoside Ro transcriptome glycosyltransferase biosynthesis
topic	misc QK1-989 misc <i<Panax ginseng</i< misc ginsenoside Ro misc transcriptome misc glycosyltransferase misc biosynthesis misc Botany
topic_unstemmed	misc QK1-989 misc <i<Panax ginseng</i< misc ginsenoside Ro misc transcriptome misc glycosyltransferase misc biosynthesis misc Botany
topic_browse	misc QK1-989 misc <i<Panax ginseng</i< misc ginsenoside Ro misc transcriptome misc glycosyltransferase misc biosynthesis misc Botany
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title	Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i<
ctrlnum	(DE-627)DOAJ091241650 (DE-599)DOAJ8f06d545accc489a9b37782611f43d42
title_full	Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i<
author_sort	Xiaochen Yu
journal	Plants
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author_browse	Xiaochen Yu Jinghui Yu Sizhang Liu Mingming Liu Kangyu Wang Mingzhu Zhao Yanfang Wang Ping Chen Jun Lei Yi Wang Meiping Zhang
container_volume	13
class	QK1-989
format_se	Elektronische Aufsätze
author-letter	Xiaochen Yu
doi_str_mv	10.3390/plants13050604
author2-role	verfasserin
title_sort	transcriptome-wide identification and integrated analysis of a <i<ugt</i< gene involved in ginsenoside ro biosynthesis in <i<panax ginseng</i<
callnumber	QK1-989
title_auth	Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i<
abstract	<i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes.
abstractGer	<i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes.
abstract_unstemmed	<i<Panax ginseng</i< as a traditional medicinal plant with a long history of medicinal use. Ginsenoside Ro is the only oleanane-type ginsenoside in ginseng, and has various pharmacological activities, including anti-inflammatory, detoxification, and antithrombotic activities. UDP-dependent glycosyltransferase (UGT) plays a key role in the synthesis of ginsenoside, and the excavation of UGT genes involved in the biosynthesis of ginsenoside Ro has great significance in enriching ginsenoside genetic resources and further revealing the synthesis mechanism of ginsenoside. In this work, ginsenoside-Ro-synthesis-related genes were mined using the <i<P. ginseng</i< reference-free transcriptome database. Fourteen hub transcripts were identified by differential expression analysis and weighted gene co-expression network analysis. Phylogenetic and synteny block analyses of <i<PgUGAT252645</i<, a <i<UGT</i< transcript among the hub transcripts, showed that <i<PgUGAT252645</i< belonged to the <i<UGT73</i< subfamily and was relatively conserved in ginseng plants. Functional analysis showed that <i<PgUGAT252645</i< encodes a glucuronosyltransferase that catalyzes the glucuronide modification of the C3 position of oleanolic acid using uridine diphosphate glucuronide as the substrate. Furthermore, the mutation at 622 bp of its open reading frame resulted in amino acid substitutions that may significantly affect the catalytic activity of the enzyme, and, as a consequence, affect the biosynthesis of ginsenoside Ro. Results of the in vitro enzyme activity assay of the heterologous expression product in <i<E. coli</i< of <i<PgUGAT252645</i< verified the above analyses. The function of <i<PgUGAT252645</i< was further verified by the result that its overexpression in ginseng adventitious roots significantly increased the content of ginsenoside Ro. The present work identified a new <i<UGT</i< gene involved in the biosynthesis of ginsenoside Ro, which not only enriches the functional genes in the ginsenoside synthesis pathway, but also provides the technical basis and theoretical basis for the in-depth excavation of ginsenoside-synthesis-related genes.
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container_issue	5, p 604
title_short	Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i<
url	https://doi.org/10.3390/plants13050604 https://doaj.org/article/8f06d545accc489a9b37782611f43d42 https://www.mdpi.com/2223-7747/13/5/604 https://doaj.org/toc/2223-7747
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Transcriptome-Wide Identification and Integrated Analysis of a <i<UGT</i< Gene Involved in Ginsenoside Ro Biosynthesis in <i<Panax ginseng</i<

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