Key microRNAs and target genes involved in regulating maturation in Lilium

Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transi...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Yao Chen [verfasserIn] Mengna Zhao [verfasserIn] Xiaoshan Wang [verfasserIn] Jinteng Cui [verfasserIn] Wei Ge [verfasserIn] Kezhong Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	lilium degradome phase transition mir156a spl3 spl16

Übergeordnetes Werk:	In: Ornamental Plant Research - Maximum Academic Press, 2024, 2(2022), 1, Seite 14
Übergeordnetes Werk:	volume:2 ; year:2022 ; number:1 ; pages:14

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.48130/OPR-2022-0009

Katalog-ID:	DOAJ09169549X

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520			\|a Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers.
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700	0		\|a Wei Ge \|e verfasserin \|4 aut
700	0		\|a Kezhong Zhang \|e verfasserin \|4 aut
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allfields	10.48130/OPR-2022-0009 doi (DE-627)DOAJ09169549X (DE-599)DOAJ9dd72bd26842444db9a1c01b066c2d95 DE-627 ger DE-627 rakwb eng QK900-989 TD194-195 Yao Chen verfasserin aut Key microRNAs and target genes involved in regulating maturation in Lilium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers. lilium degradome phase transition mir156a spl3 spl16 Plant ecology Environmental effects of industries and plants Mengna Zhao verfasserin aut Xiaoshan Wang verfasserin aut Jinteng Cui verfasserin aut Wei Ge verfasserin aut Kezhong Zhang verfasserin aut In Ornamental Plant Research Maximum Academic Press, 2024 2(2022), 1, Seite 14 (DE-627)1882454766 (DE-600)3180766-5 27692094 nnns volume:2 year:2022 number:1 pages:14 https://doi.org/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/article/9dd72bd26842444db9a1c01b066c2d95 kostenfrei https://www.maxapress.com/article/doi/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/toc/2769-2094 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 2 2022 1 14
spelling	10.48130/OPR-2022-0009 doi (DE-627)DOAJ09169549X (DE-599)DOAJ9dd72bd26842444db9a1c01b066c2d95 DE-627 ger DE-627 rakwb eng QK900-989 TD194-195 Yao Chen verfasserin aut Key microRNAs and target genes involved in regulating maturation in Lilium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers. lilium degradome phase transition mir156a spl3 spl16 Plant ecology Environmental effects of industries and plants Mengna Zhao verfasserin aut Xiaoshan Wang verfasserin aut Jinteng Cui verfasserin aut Wei Ge verfasserin aut Kezhong Zhang verfasserin aut In Ornamental Plant Research Maximum Academic Press, 2024 2(2022), 1, Seite 14 (DE-627)1882454766 (DE-600)3180766-5 27692094 nnns volume:2 year:2022 number:1 pages:14 https://doi.org/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/article/9dd72bd26842444db9a1c01b066c2d95 kostenfrei https://www.maxapress.com/article/doi/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/toc/2769-2094 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 2 2022 1 14
allfields_unstemmed	10.48130/OPR-2022-0009 doi (DE-627)DOAJ09169549X (DE-599)DOAJ9dd72bd26842444db9a1c01b066c2d95 DE-627 ger DE-627 rakwb eng QK900-989 TD194-195 Yao Chen verfasserin aut Key microRNAs and target genes involved in regulating maturation in Lilium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers. lilium degradome phase transition mir156a spl3 spl16 Plant ecology Environmental effects of industries and plants Mengna Zhao verfasserin aut Xiaoshan Wang verfasserin aut Jinteng Cui verfasserin aut Wei Ge verfasserin aut Kezhong Zhang verfasserin aut In Ornamental Plant Research Maximum Academic Press, 2024 2(2022), 1, Seite 14 (DE-627)1882454766 (DE-600)3180766-5 27692094 nnns volume:2 year:2022 number:1 pages:14 https://doi.org/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/article/9dd72bd26842444db9a1c01b066c2d95 kostenfrei https://www.maxapress.com/article/doi/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/toc/2769-2094 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 2 2022 1 14
allfieldsGer	10.48130/OPR-2022-0009 doi (DE-627)DOAJ09169549X (DE-599)DOAJ9dd72bd26842444db9a1c01b066c2d95 DE-627 ger DE-627 rakwb eng QK900-989 TD194-195 Yao Chen verfasserin aut Key microRNAs and target genes involved in regulating maturation in Lilium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers. lilium degradome phase transition mir156a spl3 spl16 Plant ecology Environmental effects of industries and plants Mengna Zhao verfasserin aut Xiaoshan Wang verfasserin aut Jinteng Cui verfasserin aut Wei Ge verfasserin aut Kezhong Zhang verfasserin aut In Ornamental Plant Research Maximum Academic Press, 2024 2(2022), 1, Seite 14 (DE-627)1882454766 (DE-600)3180766-5 27692094 nnns volume:2 year:2022 number:1 pages:14 https://doi.org/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/article/9dd72bd26842444db9a1c01b066c2d95 kostenfrei https://www.maxapress.com/article/doi/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/toc/2769-2094 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 2 2022 1 14
allfieldsSound	10.48130/OPR-2022-0009 doi (DE-627)DOAJ09169549X (DE-599)DOAJ9dd72bd26842444db9a1c01b066c2d95 DE-627 ger DE-627 rakwb eng QK900-989 TD194-195 Yao Chen verfasserin aut Key microRNAs and target genes involved in regulating maturation in Lilium 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers. lilium degradome phase transition mir156a spl3 spl16 Plant ecology Environmental effects of industries and plants Mengna Zhao verfasserin aut Xiaoshan Wang verfasserin aut Jinteng Cui verfasserin aut Wei Ge verfasserin aut Kezhong Zhang verfasserin aut In Ornamental Plant Research Maximum Academic Press, 2024 2(2022), 1, Seite 14 (DE-627)1882454766 (DE-600)3180766-5 27692094 nnns volume:2 year:2022 number:1 pages:14 https://doi.org/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/article/9dd72bd26842444db9a1c01b066c2d95 kostenfrei https://www.maxapress.com/article/doi/10.48130/OPR-2022-0009 kostenfrei https://doaj.org/toc/2769-2094 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 2 2022 1 14
language	English
source	In Ornamental Plant Research 2(2022), 1, Seite 14 volume:2 year:2022 number:1 pages:14
sourceStr	In Ornamental Plant Research 2(2022), 1, Seite 14 volume:2 year:2022 number:1 pages:14
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topic_facet	lilium degradome phase transition mir156a spl3 spl16 Plant ecology Environmental effects of industries and plants
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container_title	Ornamental Plant Research
authorswithroles_txt_mv	Yao Chen @@aut@@ Mengna Zhao @@aut@@ Xiaoshan Wang @@aut@@ Jinteng Cui @@aut@@ Wei Ge @@aut@@ Kezhong Zhang @@aut@@
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callnumber-first	Q - Science
author	Yao Chen
spellingShingle	Yao Chen misc QK900-989 misc TD194-195 misc lilium misc degradome misc phase transition misc mir156a misc spl3 misc spl16 misc Plant ecology misc Environmental effects of industries and plants Key microRNAs and target genes involved in regulating maturation in Lilium
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topic_title	QK900-989 TD194-195 Key microRNAs and target genes involved in regulating maturation in Lilium lilium degradome phase transition mir156a spl3 spl16
topic	misc QK900-989 misc TD194-195 misc lilium misc degradome misc phase transition misc mir156a misc spl3 misc spl16 misc Plant ecology misc Environmental effects of industries and plants
topic_unstemmed	misc QK900-989 misc TD194-195 misc lilium misc degradome misc phase transition misc mir156a misc spl3 misc spl16 misc Plant ecology misc Environmental effects of industries and plants
topic_browse	misc QK900-989 misc TD194-195 misc lilium misc degradome misc phase transition misc mir156a misc spl3 misc spl16 misc Plant ecology misc Environmental effects of industries and plants
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title	Key microRNAs and target genes involved in regulating maturation in Lilium
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title_full	Key microRNAs and target genes involved in regulating maturation in Lilium
author_sort	Yao Chen
journal	Ornamental Plant Research
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author_browse	Yao Chen Mengna Zhao Xiaoshan Wang Jinteng Cui Wei Ge Kezhong Zhang
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title_sort	key micrornas and target genes involved in regulating maturation in lilium
callnumber	QK900-989
title_auth	Key microRNAs and target genes involved in regulating maturation in Lilium
abstract	Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers.
abstractGer	Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers.
abstract_unstemmed	Lilium is an ornamental bulb with a long juvenile stage, making its cultivation under natural conditions lengthy and costly. SQUAMOSA promoter-binding protein-like (SPL) transcription factors are related to plant growth and development, including phase transition. However, their role in phase transition in Lilium is not known. To explore the molecular mechanisms associated with the phase transition in Lilium, bulbs of Lilium Oriental Trumpet 'Robina' were treated with lowered temperature to induce phase transition, and the small RNA and degradome were sequenced. A total of 161 miRNAs were identified as targets. Twenty-nine known miRNAs were differentially expressed, including 16 up-regulated miRNAs and 13 down-regulated miRNAs. Lbr-miR156a was significantly down-regulated, and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs. Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity. The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment. Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change, while the overexpression of Lbr-miR156a could inhibit this process. These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700
container_issue	1
title_short	Key microRNAs and target genes involved in regulating maturation in Lilium
url	https://doi.org/10.48130/OPR-2022-0009 https://doaj.org/article/9dd72bd26842444db9a1c01b066c2d95 https://www.maxapress.com/article/doi/10.48130/OPR-2022-0009 https://doaj.org/toc/2769-2094
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author2	Mengna Zhao Xiaoshan Wang Jinteng Cui Wei Ge Kezhong Zhang
author2Str	Mengna Zhao Xiaoshan Wang Jinteng Cui Wei Ge Kezhong Zhang
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doi_str	10.48130/OPR-2022-0009
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up_date	2024-07-03T21:43:30.754Z
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Key microRNAs and target genes involved in regulating maturation in Lilium

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