Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis

Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly impact on patients’ quality of life. Enteric glial cells (EGC) are the key cell type of enteric nervous system (ENS), which contributes to the destruction of gut homeostasis in DM. Circular RNAs (circRNAs) are a novel type o...
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Gespeichert in:

Autor*in:	Zhu Xiaowei [verfasserIn] Li Yanyu [verfasserIn] Zhu Xuping [verfasserIn] Wang Ke [verfasserIn] Zhu Xue [verfasserIn] Jiang Yanmin [verfasserIn] Xu Lan [verfasserIn] Li Jianbo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	diabetes mellitus gastrointestinal complications enteric glia circVPS13A

Übergeordnetes Werk:	In: Acta Biochimica et Biophysica Sinica - China Science Publishing & Media Ltd., 2023, 54(2022), Seite 999-1007
Übergeordnetes Werk:	volume:54 ; year:2022 ; pages:999-1007

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3724/abbs.2022073

Katalog-ID:	DOAJ092001599

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520			\|a Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly impact on patients’ quality of life. Enteric glial cells (EGC) are the key cell type of enteric nervous system (ENS), which contributes to the destruction of gut homeostasis in DM. Circular RNAs (circRNAs) are a novel type of RNAs abundant in the eukaryotic transcriptome, which form covalently closed continuous loops. In this study, the contribution of circRNAs to EGC damage in DM is investigated. Transcriptome sequencing analysis and functional study show that circVPS13A is significantly down-regulated in hyperglycemia-treated EGC, and circVPS13A overexpression attenuates EGC damage in both in vitro and in vivo DM models. In vitro mechanistic study using dual-luciferase reporter assay, affinity-isolation assay, fluorescence in situ hybridization (FISH) and immunostaining analysis identify that circVPS13A exerts its protective effect by sponging miR-182 and then up-regulates glial cell line-derived neurotrophic factor (GDNF) expression. In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network regulation can attenuate hyperglycemia-induced EGC damage of duodenum in streptozotocine (STZ)-induced DM mice. The findings of this study may provide novel insights into the protective role of circVPS13A in DM-associated EGC damage and clues for the development of new therapeutic approaches for the prevention of GI complications of DM.
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allfields	10.3724/abbs.2022073 doi (DE-627)DOAJ092001599 (DE-599)DOAJ3bd46c0984ad4207b66b91b129927379 DE-627 ger DE-627 rakwb eng QD415-436 QH426-470 Zhu Xiaowei verfasserin aut Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly impact on patients’ quality of life. Enteric glial cells (EGC) are the key cell type of enteric nervous system (ENS), which contributes to the destruction of gut homeostasis in DM. Circular RNAs (circRNAs) are a novel type of RNAs abundant in the eukaryotic transcriptome, which form covalently closed continuous loops. In this study, the contribution of circRNAs to EGC damage in DM is investigated. Transcriptome sequencing analysis and functional study show that circVPS13A is significantly down-regulated in hyperglycemia-treated EGC, and circVPS13A overexpression attenuates EGC damage in both in vitro and in vivo DM models. In vitro mechanistic study using dual-luciferase reporter assay, affinity-isolation assay, fluorescence in situ hybridization (FISH) and immunostaining analysis identify that circVPS13A exerts its protective effect by sponging miR-182 and then up-regulates glial cell line-derived neurotrophic factor (GDNF) expression. In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network regulation can attenuate hyperglycemia-induced EGC damage of duodenum in streptozotocine (STZ)-induced DM mice. The findings of this study may provide novel insights into the protective role of circVPS13A in DM-associated EGC damage and clues for the development of new therapeutic approaches for the prevention of GI complications of DM. diabetes mellitus gastrointestinal complications enteric glia circVPS13A Biochemistry Genetics Li Yanyu verfasserin aut Zhu Xuping verfasserin aut Wang Ke verfasserin aut Zhu Xue verfasserin aut Jiang Yanmin verfasserin aut Xu Lan verfasserin aut Li Jianbo verfasserin aut In Acta Biochimica et Biophysica Sinica China Science Publishing & Media Ltd., 2023 54(2022), Seite 999-1007 (DE-627)478504861 (DE-600)2175256-4 17457270 nnns volume:54 year:2022 pages:999-1007 https://doi.org/10.3724/abbs.2022073 kostenfrei https://doaj.org/article/3bd46c0984ad4207b66b91b129927379 kostenfrei https://www.sciengine.com/doi/10.3724/abbs.2022073 kostenfrei https://doaj.org/toc/1672-9145 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 54 2022 999-1007
spelling	10.3724/abbs.2022073 doi (DE-627)DOAJ092001599 (DE-599)DOAJ3bd46c0984ad4207b66b91b129927379 DE-627 ger DE-627 rakwb eng QD415-436 QH426-470 Zhu Xiaowei verfasserin aut Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly impact on patients’ quality of life. Enteric glial cells (EGC) are the key cell type of enteric nervous system (ENS), which contributes to the destruction of gut homeostasis in DM. Circular RNAs (circRNAs) are a novel type of RNAs abundant in the eukaryotic transcriptome, which form covalently closed continuous loops. In this study, the contribution of circRNAs to EGC damage in DM is investigated. Transcriptome sequencing analysis and functional study show that circVPS13A is significantly down-regulated in hyperglycemia-treated EGC, and circVPS13A overexpression attenuates EGC damage in both in vitro and in vivo DM models. In vitro mechanistic study using dual-luciferase reporter assay, affinity-isolation assay, fluorescence in situ hybridization (FISH) and immunostaining analysis identify that circVPS13A exerts its protective effect by sponging miR-182 and then up-regulates glial cell line-derived neurotrophic factor (GDNF) expression. In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network regulation can attenuate hyperglycemia-induced EGC damage of duodenum in streptozotocine (STZ)-induced DM mice. The findings of this study may provide novel insights into the protective role of circVPS13A in DM-associated EGC damage and clues for the development of new therapeutic approaches for the prevention of GI complications of DM. diabetes mellitus gastrointestinal complications enteric glia circVPS13A Biochemistry Genetics Li Yanyu verfasserin aut Zhu Xuping verfasserin aut Wang Ke verfasserin aut Zhu Xue verfasserin aut Jiang Yanmin verfasserin aut Xu Lan verfasserin aut Li Jianbo verfasserin aut In Acta Biochimica et Biophysica Sinica China Science Publishing & Media Ltd., 2023 54(2022), Seite 999-1007 (DE-627)478504861 (DE-600)2175256-4 17457270 nnns volume:54 year:2022 pages:999-1007 https://doi.org/10.3724/abbs.2022073 kostenfrei https://doaj.org/article/3bd46c0984ad4207b66b91b129927379 kostenfrei https://www.sciengine.com/doi/10.3724/abbs.2022073 kostenfrei https://doaj.org/toc/1672-9145 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 54 2022 999-1007
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allfieldsGer	10.3724/abbs.2022073 doi (DE-627)DOAJ092001599 (DE-599)DOAJ3bd46c0984ad4207b66b91b129927379 DE-627 ger DE-627 rakwb eng QD415-436 QH426-470 Zhu Xiaowei verfasserin aut Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly impact on patients’ quality of life. Enteric glial cells (EGC) are the key cell type of enteric nervous system (ENS), which contributes to the destruction of gut homeostasis in DM. Circular RNAs (circRNAs) are a novel type of RNAs abundant in the eukaryotic transcriptome, which form covalently closed continuous loops. In this study, the contribution of circRNAs to EGC damage in DM is investigated. Transcriptome sequencing analysis and functional study show that circVPS13A is significantly down-regulated in hyperglycemia-treated EGC, and circVPS13A overexpression attenuates EGC damage in both in vitro and in vivo DM models. In vitro mechanistic study using dual-luciferase reporter assay, affinity-isolation assay, fluorescence in situ hybridization (FISH) and immunostaining analysis identify that circVPS13A exerts its protective effect by sponging miR-182 and then up-regulates glial cell line-derived neurotrophic factor (GDNF) expression. In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network regulation can attenuate hyperglycemia-induced EGC damage of duodenum in streptozotocine (STZ)-induced DM mice. The findings of this study may provide novel insights into the protective role of circVPS13A in DM-associated EGC damage and clues for the development of new therapeutic approaches for the prevention of GI complications of DM. diabetes mellitus gastrointestinal complications enteric glia circVPS13A Biochemistry Genetics Li Yanyu verfasserin aut Zhu Xuping verfasserin aut Wang Ke verfasserin aut Zhu Xue verfasserin aut Jiang Yanmin verfasserin aut Xu Lan verfasserin aut Li Jianbo verfasserin aut In Acta Biochimica et Biophysica Sinica China Science Publishing & Media Ltd., 2023 54(2022), Seite 999-1007 (DE-627)478504861 (DE-600)2175256-4 17457270 nnns volume:54 year:2022 pages:999-1007 https://doi.org/10.3724/abbs.2022073 kostenfrei https://doaj.org/article/3bd46c0984ad4207b66b91b129927379 kostenfrei https://www.sciengine.com/doi/10.3724/abbs.2022073 kostenfrei https://doaj.org/toc/1672-9145 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 54 2022 999-1007
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source	In Acta Biochimica et Biophysica Sinica 54(2022), Seite 999-1007 volume:54 year:2022 pages:999-1007
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callnumber-first	Q - Science
author	Zhu Xiaowei
spellingShingle	Zhu Xiaowei misc QD415-436 misc QH426-470 misc diabetes mellitus misc gastrointestinal complications misc enteric glia misc circVPS13A misc Biochemistry misc Genetics Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis
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topic_title	QD415-436 QH426-470 Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis diabetes mellitus gastrointestinal complications enteric glia circVPS13A
topic	misc QD415-436 misc QH426-470 misc diabetes mellitus misc gastrointestinal complications misc enteric glia misc circVPS13A misc Biochemistry misc Genetics
topic_unstemmed	misc QD415-436 misc QH426-470 misc diabetes mellitus misc gastrointestinal complications misc enteric glia misc circVPS13A misc Biochemistry misc Genetics
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title	Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis
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title_full	Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis
author_sort	Zhu Xiaowei
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title_sort	circular rna-vps13a attenuates diabetes-induced enteric glia damage by targeting mir-182/gdnf axis
callnumber	QD415-436
title_auth	Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis
abstract	Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly impact on patients’ quality of life. Enteric glial cells (EGC) are the key cell type of enteric nervous system (ENS), which contributes to the destruction of gut homeostasis in DM. Circular RNAs (circRNAs) are a novel type of RNAs abundant in the eukaryotic transcriptome, which form covalently closed continuous loops. In this study, the contribution of circRNAs to EGC damage in DM is investigated. Transcriptome sequencing analysis and functional study show that circVPS13A is significantly down-regulated in hyperglycemia-treated EGC, and circVPS13A overexpression attenuates EGC damage in both in vitro and in vivo DM models. In vitro mechanistic study using dual-luciferase reporter assay, affinity-isolation assay, fluorescence in situ hybridization (FISH) and immunostaining analysis identify that circVPS13A exerts its protective effect by sponging miR-182 and then up-regulates glial cell line-derived neurotrophic factor (GDNF) expression. In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network regulation can attenuate hyperglycemia-induced EGC damage of duodenum in streptozotocine (STZ)-induced DM mice. The findings of this study may provide novel insights into the protective role of circVPS13A in DM-associated EGC damage and clues for the development of new therapeutic approaches for the prevention of GI complications of DM.
abstractGer	Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly impact on patients’ quality of life. Enteric glial cells (EGC) are the key cell type of enteric nervous system (ENS), which contributes to the destruction of gut homeostasis in DM. Circular RNAs (circRNAs) are a novel type of RNAs abundant in the eukaryotic transcriptome, which form covalently closed continuous loops. In this study, the contribution of circRNAs to EGC damage in DM is investigated. Transcriptome sequencing analysis and functional study show that circVPS13A is significantly down-regulated in hyperglycemia-treated EGC, and circVPS13A overexpression attenuates EGC damage in both in vitro and in vivo DM models. In vitro mechanistic study using dual-luciferase reporter assay, affinity-isolation assay, fluorescence in situ hybridization (FISH) and immunostaining analysis identify that circVPS13A exerts its protective effect by sponging miR-182 and then up-regulates glial cell line-derived neurotrophic factor (GDNF) expression. In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network regulation can attenuate hyperglycemia-induced EGC damage of duodenum in streptozotocine (STZ)-induced DM mice. The findings of this study may provide novel insights into the protective role of circVPS13A in DM-associated EGC damage and clues for the development of new therapeutic approaches for the prevention of GI complications of DM.
abstract_unstemmed	Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly impact on patients’ quality of life. Enteric glial cells (EGC) are the key cell type of enteric nervous system (ENS), which contributes to the destruction of gut homeostasis in DM. Circular RNAs (circRNAs) are a novel type of RNAs abundant in the eukaryotic transcriptome, which form covalently closed continuous loops. In this study, the contribution of circRNAs to EGC damage in DM is investigated. Transcriptome sequencing analysis and functional study show that circVPS13A is significantly down-regulated in hyperglycemia-treated EGC, and circVPS13A overexpression attenuates EGC damage in both in vitro and in vivo DM models. In vitro mechanistic study using dual-luciferase reporter assay, affinity-isolation assay, fluorescence in situ hybridization (FISH) and immunostaining analysis identify that circVPS13A exerts its protective effect by sponging miR-182 and then up-regulates glial cell line-derived neurotrophic factor (GDNF) expression. In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network regulation can attenuate hyperglycemia-induced EGC damage of duodenum in streptozotocine (STZ)-induced DM mice. The findings of this study may provide novel insights into the protective role of circVPS13A in DM-associated EGC damage and clues for the development of new therapeutic approaches for the prevention of GI complications of DM.
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title_short	Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis
url	https://doi.org/10.3724/abbs.2022073 https://doaj.org/article/3bd46c0984ad4207b66b91b129927379 https://www.sciengine.com/doi/10.3724/abbs.2022073 https://doaj.org/toc/1672-9145
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author2	Li Yanyu Zhu Xuping Wang Ke Zhu Xue Jiang Yanmin Xu Lan Li Jianbo
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doi_str	10.3724/abbs.2022073
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up_date	2024-07-03T23:27:58.451Z
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In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network regulation can attenuate hyperglycemia-induced EGC damage of duodenum in streptozotocine (STZ)-induced DM mice. 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Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting miR-182/GDNF axis

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