Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland

Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibilit...
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Autor*in:	Dorota Kamińska [verfasserIn] Magdalena Ratajczak [verfasserIn] Dorota M. Nowak-Malczewska [verfasserIn] Justyna A. Karolak [verfasserIn] Marek Kwaśniewski [verfasserIn] Anna Szumala-Kakol [verfasserIn] Jolanta Dlugaszewska [verfasserIn] Marzena Gajecka [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Group B Streptococcus Macrolide resistance Lincosamide resistance Streptogramin resistance Antimicrobial susceptibility patterns Serotyping

Übergeordnetes Werk:	In: Scientific Reports - Nature Portfolio, 2011, 14(2024), 1, Seite 8
Übergeordnetes Werk:	volume:14 ; year:2024 ; number:1 ; pages:8

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1038/s41598-024-54521-y

Katalog-ID:	DOAJ092160557

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allfields	10.1038/s41598-024-54521-y doi (DE-627)DOAJ092160557 (DE-599)DOAJdc76744c1b0c4e24adbf6a4f93343598 DE-627 ger DE-627 rakwb eng Dorota Kamińska verfasserin aut Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLSB mechanism (78.2%), inducible MLSB mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone. Group B Streptococcus Macrolide resistance Lincosamide resistance Streptogramin resistance Antimicrobial susceptibility patterns Serotyping Medicine R Science Q Magdalena Ratajczak verfasserin aut Dorota M. Nowak-Malczewska verfasserin aut Justyna A. Karolak verfasserin aut Marek Kwaśniewski verfasserin aut Anna Szumala-Kakol verfasserin aut Jolanta Dlugaszewska verfasserin aut Marzena Gajecka verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 8 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:8 https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/article/dc76744c1b0c4e24adbf6a4f93343598 kostenfrei https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 8
spelling	10.1038/s41598-024-54521-y doi (DE-627)DOAJ092160557 (DE-599)DOAJdc76744c1b0c4e24adbf6a4f93343598 DE-627 ger DE-627 rakwb eng Dorota Kamińska verfasserin aut Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLSB mechanism (78.2%), inducible MLSB mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone. Group B Streptococcus Macrolide resistance Lincosamide resistance Streptogramin resistance Antimicrobial susceptibility patterns Serotyping Medicine R Science Q Magdalena Ratajczak verfasserin aut Dorota M. Nowak-Malczewska verfasserin aut Justyna A. Karolak verfasserin aut Marek Kwaśniewski verfasserin aut Anna Szumala-Kakol verfasserin aut Jolanta Dlugaszewska verfasserin aut Marzena Gajecka verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 8 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:8 https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/article/dc76744c1b0c4e24adbf6a4f93343598 kostenfrei https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 8
allfields_unstemmed	10.1038/s41598-024-54521-y doi (DE-627)DOAJ092160557 (DE-599)DOAJdc76744c1b0c4e24adbf6a4f93343598 DE-627 ger DE-627 rakwb eng Dorota Kamińska verfasserin aut Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLSB mechanism (78.2%), inducible MLSB mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone. Group B Streptococcus Macrolide resistance Lincosamide resistance Streptogramin resistance Antimicrobial susceptibility patterns Serotyping Medicine R Science Q Magdalena Ratajczak verfasserin aut Dorota M. Nowak-Malczewska verfasserin aut Justyna A. Karolak verfasserin aut Marek Kwaśniewski verfasserin aut Anna Szumala-Kakol verfasserin aut Jolanta Dlugaszewska verfasserin aut Marzena Gajecka verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 8 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:8 https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/article/dc76744c1b0c4e24adbf6a4f93343598 kostenfrei https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 8
allfieldsGer	10.1038/s41598-024-54521-y doi (DE-627)DOAJ092160557 (DE-599)DOAJdc76744c1b0c4e24adbf6a4f93343598 DE-627 ger DE-627 rakwb eng Dorota Kamińska verfasserin aut Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLSB mechanism (78.2%), inducible MLSB mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone. Group B Streptococcus Macrolide resistance Lincosamide resistance Streptogramin resistance Antimicrobial susceptibility patterns Serotyping Medicine R Science Q Magdalena Ratajczak verfasserin aut Dorota M. Nowak-Malczewska verfasserin aut Justyna A. Karolak verfasserin aut Marek Kwaśniewski verfasserin aut Anna Szumala-Kakol verfasserin aut Jolanta Dlugaszewska verfasserin aut Marzena Gajecka verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 8 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:8 https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/article/dc76744c1b0c4e24adbf6a4f93343598 kostenfrei https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 8
allfieldsSound	10.1038/s41598-024-54521-y doi (DE-627)DOAJ092160557 (DE-599)DOAJdc76744c1b0c4e24adbf6a4f93343598 DE-627 ger DE-627 rakwb eng Dorota Kamińska verfasserin aut Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLSB mechanism (78.2%), inducible MLSB mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone. Group B Streptococcus Macrolide resistance Lincosamide resistance Streptogramin resistance Antimicrobial susceptibility patterns Serotyping Medicine R Science Q Magdalena Ratajczak verfasserin aut Dorota M. Nowak-Malczewska verfasserin aut Justyna A. Karolak verfasserin aut Marek Kwaśniewski verfasserin aut Anna Szumala-Kakol verfasserin aut Jolanta Dlugaszewska verfasserin aut Marzena Gajecka verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 8 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:8 https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/article/dc76744c1b0c4e24adbf6a4f93343598 kostenfrei https://doi.org/10.1038/s41598-024-54521-y kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 8
language	English
source	In Scientific Reports 14(2024), 1, Seite 8 volume:14 year:2024 number:1 pages:8
sourceStr	In Scientific Reports 14(2024), 1, Seite 8 volume:14 year:2024 number:1 pages:8
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Group B Streptococcus Macrolide resistance Lincosamide resistance Streptogramin resistance Antimicrobial susceptibility patterns Serotyping Medicine R Science Q
isfreeaccess_bool	true
container_title	Scientific Reports
authorswithroles_txt_mv	Dorota Kamińska @@aut@@ Magdalena Ratajczak @@aut@@ Dorota M. Nowak-Malczewska @@aut@@ Justyna A. Karolak @@aut@@ Marek Kwaśniewski @@aut@@ Anna Szumala-Kakol @@aut@@ Jolanta Dlugaszewska @@aut@@ Marzena Gajecka @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	663366712
id	DOAJ092160557
language_de	englisch
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author	Dorota Kamińska
spellingShingle	Dorota Kamińska misc Group B Streptococcus misc Macrolide resistance misc Lincosamide resistance misc Streptogramin resistance misc Antimicrobial susceptibility patterns misc Serotyping misc Medicine misc R misc Science misc Q Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland
authorStr	Dorota Kamińska
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topic_title	Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland Group B Streptococcus Macrolide resistance Lincosamide resistance Streptogramin resistance Antimicrobial susceptibility patterns Serotyping
topic	misc Group B Streptococcus misc Macrolide resistance misc Lincosamide resistance misc Streptogramin resistance misc Antimicrobial susceptibility patterns misc Serotyping misc Medicine misc R misc Science misc Q
topic_unstemmed	misc Group B Streptococcus misc Macrolide resistance misc Lincosamide resistance misc Streptogramin resistance misc Antimicrobial susceptibility patterns misc Serotyping misc Medicine misc R misc Science misc Q
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title	Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland
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title_full	Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland
author_sort	Dorota Kamińska
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author_browse	Dorota Kamińska Magdalena Ratajczak Dorota M. Nowak-Malczewska Justyna A. Karolak Marek Kwaśniewski Anna Szumala-Kakol Jolanta Dlugaszewska Marzena Gajecka
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title_sort	macrolide and lincosamide resistance of streptococcus agalactiae in pregnant women in poland
title_auth	Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland
abstract	Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLSB mechanism (78.2%), inducible MLSB mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone.
abstractGer	Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLSB mechanism (78.2%), inducible MLSB mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone.
abstract_unstemmed	Abstract Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLSB mechanism (78.2%), inducible MLSB mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone.
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title_short	Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland
url	https://doi.org/10.1038/s41598-024-54521-y https://doaj.org/article/dc76744c1b0c4e24adbf6a4f93343598 https://doaj.org/toc/2045-2322
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author2Str	Magdalena Ratajczak Dorota M. Nowak-Malczewska Justyna A. Karolak Marek Kwaśniewski Anna Szumala-Kakol Jolanta Dlugaszewska Marzena Gajecka
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Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland

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Zugang & Verfügbarkeit

Vorhandene Bände

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