Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli

Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as t...
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Autor*in:	Ole Madsen [verfasserIn] Roxann S.C. Rikkers [verfasserIn] Jerry M. Wells [verfasserIn] Rob Bergsma [verfasserIn] Soumya K. Kar [verfasserIn] Nico Taverne [verfasserIn] Anja J. Taverne-Thiele [verfasserIn] Esther D. Ellen [verfasserIn] Henri Woelders [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Feed efficiency Gene expression Immunity Intestinal organoids Pigs

Übergeordnetes Werk:	In: BMC Genomics - BMC, 2003, 25(2024), 1, Seite 17
Übergeordnetes Werk:	volume:25 ; year:2024 ; number:1 ; pages:17

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12864-024-10064-0

Katalog-ID:	DOAJ092184243

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520			\|a Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE.
650		4	\|a Feed efficiency
650		4	\|a Gene expression
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650		4	\|a Intestinal organoids
650		4	\|a Pigs
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700	0		\|a Anja J. Taverne-Thiele \|e verfasserin \|4 aut
700	0		\|a Esther D. Ellen \|e verfasserin \|4 aut
700	0		\|a Henri Woelders \|e verfasserin \|4 aut
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author_variant	o m om r s r rsr j m w jmw r b rb s k k skk n t nt a j t t ajtt e d e ede h w hw
matchkey_str	article:14712164:2024----::rncitmcnlssfnetnlraoddrvdrmisiegnifeefcecad
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publishDate	2024
allfields	10.1186/s12864-024-10064-0 doi (DE-627)DOAJ092184243 (DE-599)DOAJb973fd59652944b992bee4c6300870fa DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Ole Madsen verfasserin aut Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE. Feed efficiency Gene expression Immunity Intestinal organoids Pigs Biotechnology Genetics Roxann S.C. Rikkers verfasserin aut Jerry M. Wells verfasserin aut Rob Bergsma verfasserin aut Soumya K. Kar verfasserin aut Nico Taverne verfasserin aut Anja J. Taverne-Thiele verfasserin aut Esther D. Ellen verfasserin aut Henri Woelders verfasserin aut In BMC Genomics BMC, 2003 25(2024), 1, Seite 17 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:25 year:2024 number:1 pages:17 https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/article/b973fd59652944b992bee4c6300870fa kostenfrei https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 1 17
spelling	10.1186/s12864-024-10064-0 doi (DE-627)DOAJ092184243 (DE-599)DOAJb973fd59652944b992bee4c6300870fa DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Ole Madsen verfasserin aut Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE. Feed efficiency Gene expression Immunity Intestinal organoids Pigs Biotechnology Genetics Roxann S.C. Rikkers verfasserin aut Jerry M. Wells verfasserin aut Rob Bergsma verfasserin aut Soumya K. Kar verfasserin aut Nico Taverne verfasserin aut Anja J. Taverne-Thiele verfasserin aut Esther D. Ellen verfasserin aut Henri Woelders verfasserin aut In BMC Genomics BMC, 2003 25(2024), 1, Seite 17 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:25 year:2024 number:1 pages:17 https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/article/b973fd59652944b992bee4c6300870fa kostenfrei https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 1 17
allfields_unstemmed	10.1186/s12864-024-10064-0 doi (DE-627)DOAJ092184243 (DE-599)DOAJb973fd59652944b992bee4c6300870fa DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Ole Madsen verfasserin aut Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE. Feed efficiency Gene expression Immunity Intestinal organoids Pigs Biotechnology Genetics Roxann S.C. Rikkers verfasserin aut Jerry M. Wells verfasserin aut Rob Bergsma verfasserin aut Soumya K. Kar verfasserin aut Nico Taverne verfasserin aut Anja J. Taverne-Thiele verfasserin aut Esther D. Ellen verfasserin aut Henri Woelders verfasserin aut In BMC Genomics BMC, 2003 25(2024), 1, Seite 17 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:25 year:2024 number:1 pages:17 https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/article/b973fd59652944b992bee4c6300870fa kostenfrei https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 1 17
allfieldsGer	10.1186/s12864-024-10064-0 doi (DE-627)DOAJ092184243 (DE-599)DOAJb973fd59652944b992bee4c6300870fa DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Ole Madsen verfasserin aut Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE. Feed efficiency Gene expression Immunity Intestinal organoids Pigs Biotechnology Genetics Roxann S.C. Rikkers verfasserin aut Jerry M. Wells verfasserin aut Rob Bergsma verfasserin aut Soumya K. Kar verfasserin aut Nico Taverne verfasserin aut Anja J. Taverne-Thiele verfasserin aut Esther D. Ellen verfasserin aut Henri Woelders verfasserin aut In BMC Genomics BMC, 2003 25(2024), 1, Seite 17 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:25 year:2024 number:1 pages:17 https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/article/b973fd59652944b992bee4c6300870fa kostenfrei https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 1 17
allfieldsSound	10.1186/s12864-024-10064-0 doi (DE-627)DOAJ092184243 (DE-599)DOAJb973fd59652944b992bee4c6300870fa DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Ole Madsen verfasserin aut Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE. Feed efficiency Gene expression Immunity Intestinal organoids Pigs Biotechnology Genetics Roxann S.C. Rikkers verfasserin aut Jerry M. Wells verfasserin aut Rob Bergsma verfasserin aut Soumya K. Kar verfasserin aut Nico Taverne verfasserin aut Anja J. Taverne-Thiele verfasserin aut Esther D. Ellen verfasserin aut Henri Woelders verfasserin aut In BMC Genomics BMC, 2003 25(2024), 1, Seite 17 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:25 year:2024 number:1 pages:17 https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/article/b973fd59652944b992bee4c6300870fa kostenfrei https://doi.org/10.1186/s12864-024-10064-0 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 1 17
language	English
source	In BMC Genomics 25(2024), 1, Seite 17 volume:25 year:2024 number:1 pages:17
sourceStr	In BMC Genomics 25(2024), 1, Seite 17 volume:25 year:2024 number:1 pages:17
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Feed efficiency Gene expression Immunity Intestinal organoids Pigs Biotechnology Genetics
isfreeaccess_bool	true
container_title	BMC Genomics
authorswithroles_txt_mv	Ole Madsen @@aut@@ Roxann S.C. Rikkers @@aut@@ Jerry M. Wells @@aut@@ Rob Bergsma @@aut@@ Soumya K. Kar @@aut@@ Nico Taverne @@aut@@ Anja J. Taverne-Thiele @@aut@@ Esther D. Ellen @@aut@@ Henri Woelders @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	326644954
id	DOAJ092184243
language_de	englisch
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callnumber-first	T - Technology
author	Ole Madsen
spellingShingle	Ole Madsen misc TP248.13-248.65 misc QH426-470 misc Feed efficiency misc Gene expression misc Immunity misc Intestinal organoids misc Pigs misc Biotechnology misc Genetics Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli
authorStr	Ole Madsen
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topic_title	TP248.13-248.65 QH426-470 Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli Feed efficiency Gene expression Immunity Intestinal organoids Pigs
topic	misc TP248.13-248.65 misc QH426-470 misc Feed efficiency misc Gene expression misc Immunity misc Intestinal organoids misc Pigs misc Biotechnology misc Genetics
topic_unstemmed	misc TP248.13-248.65 misc QH426-470 misc Feed efficiency misc Gene expression misc Immunity misc Intestinal organoids misc Pigs misc Biotechnology misc Genetics
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title	Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli
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title_full	Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli
author_sort	Ole Madsen
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author_browse	Ole Madsen Roxann S.C. Rikkers Jerry M. Wells Rob Bergsma Soumya K. Kar Nico Taverne Anja J. Taverne-Thiele Esther D. Ellen Henri Woelders
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title_sort	transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to escherichia coli
callnumber	TP248.13-248.65
title_auth	Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli
abstract	Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE.
abstractGer	Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE.
abstract_unstemmed	Abstract Background There is increasing interest in using intestinal organoids to study complex traits like feed efficiency (FE) and host-microbe interactions. The aim of this study was to investigate differences in the molecular phenotype of organoids derived from pigs divergent for FE as well as their responses to challenge with adherent and invasive Escherichia coli (E. coli). Results Colon and ileum tissue from low and high FE pigs was used to generate 3D organoids and two dimensional (2D) monolayers of organoid cells for E. coli challenge. Genome-wide gene expression was used to investigate molecular differences between pigs that were phenotypically divergent for FE and to study the difference in gene expression after challenge with E. coli. We showed, (1) minor differences in gene expression of colon organoids from pigs with low and high FE phenotypes, (2) that an E. coli challenge results in a strong innate immune gene response in both colon and ileum organoids, (3) that the immune response seems to be less pronounced in the colon organoids of high FE pigs and (4) a slightly stronger immune response was observed in ileum than in colon organoids. Conclusions These findings demonstrate the potential for using organoids to gain insights into complex biological mechanisms such as FE.
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container_issue	1
title_short	Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli
url	https://doi.org/10.1186/s12864-024-10064-0 https://doaj.org/article/b973fd59652944b992bee4c6300870fa https://doaj.org/toc/1471-2164
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author2	Roxann S.C. Rikkers Jerry M. Wells Rob Bergsma Soumya K. Kar Nico Taverne Anja J. Taverne-Thiele Esther D. Ellen Henri Woelders
author2Str	Roxann S.C. Rikkers Jerry M. Wells Rob Bergsma Soumya K. Kar Nico Taverne Anja J. Taverne-Thiele Esther D. Ellen Henri Woelders
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up_date	2024-07-04T00:17:15.986Z
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Transcriptomic analysis of intestinal organoids, derived from pigs divergent in feed efficiency, and their response to Escherichia coli

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