Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer

Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jamal Moideen Muthu Mohamed [verfasserIn] Fazil Ahmad [verfasserIn] Mohamed El-Sherbiny [verfasserIn] Mohammed Ahmad Al Mohaini [verfasserIn] Krishnaraju Venkatesan [verfasserIn] Yahya Bin Abdullah Alrashdi [verfasserIn] Mamdouh Basheir Eldesoqui [verfasserIn] Adel Ehab Ibrahim [verfasserIn] Amal Fahmy Dawood [verfasserIn] Ateya Megahed Ibrahim [verfasserIn] Sami El Deeb [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Ultrasonication Quercetin MTT assay Box–Behnken design Anticancer Solid lipid nanoparticles

Übergeordnetes Werk:	In: Cancer Nanotechnology - BMC, 2017, 15(2024), 1, Seite 17
Übergeordnetes Werk:	volume:15 ; year:2024 ; number:1 ; pages:17

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1186/s12645-024-00249-3

Katalog-ID:	DOAJ092188664

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245	1	0	\|a Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer
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520			\|a Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC.
650		4	\|a Ultrasonication
650		4	\|a Quercetin
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650		4	\|a Anticancer
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653		0	\|a Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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700	0		\|a Krishnaraju Venkatesan \|e verfasserin \|4 aut
700	0		\|a Yahya Bin Abdullah Alrashdi \|e verfasserin \|4 aut
700	0		\|a Mamdouh Basheir Eldesoqui \|e verfasserin \|4 aut
700	0		\|a Adel Ehab Ibrahim \|e verfasserin \|4 aut
700	0		\|a Amal Fahmy Dawood \|e verfasserin \|4 aut
700	0		\|a Ateya Megahed Ibrahim \|e verfasserin \|4 aut
700	0		\|a Sami El Deeb \|e verfasserin \|4 aut
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allfields	10.1186/s12645-024-00249-3 doi (DE-627)DOAJ092188664 (DE-599)DOAJ9732a9626ac242ee9a27e064db1c6b29 DE-627 ger DE-627 rakwb eng RC254-282 Jamal Moideen Muthu Mohamed verfasserin aut Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC. Ultrasonication Quercetin MTT assay Box–Behnken design Anticancer Solid lipid nanoparticles Neoplasms. Tumors. Oncology. Including cancer and carcinogens Fazil Ahmad verfasserin aut Mohamed El-Sherbiny verfasserin aut Mohammed Ahmad Al Mohaini verfasserin aut Krishnaraju Venkatesan verfasserin aut Yahya Bin Abdullah Alrashdi verfasserin aut Mamdouh Basheir Eldesoqui verfasserin aut Adel Ehab Ibrahim verfasserin aut Amal Fahmy Dawood verfasserin aut Ateya Megahed Ibrahim verfasserin aut Sami El Deeb verfasserin aut In Cancer Nanotechnology BMC, 2017 15(2024), 1, Seite 17 (DE-627)626052327 (DE-600)2553049-5 18686966 nnns volume:15 year:2024 number:1 pages:17 https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/article/9732a9626ac242ee9a27e064db1c6b29 kostenfrei https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/toc/1868-6958 Journal toc kostenfrei https://doaj.org/toc/1868-6966 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 17
spelling	10.1186/s12645-024-00249-3 doi (DE-627)DOAJ092188664 (DE-599)DOAJ9732a9626ac242ee9a27e064db1c6b29 DE-627 ger DE-627 rakwb eng RC254-282 Jamal Moideen Muthu Mohamed verfasserin aut Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC. Ultrasonication Quercetin MTT assay Box–Behnken design Anticancer Solid lipid nanoparticles Neoplasms. Tumors. Oncology. Including cancer and carcinogens Fazil Ahmad verfasserin aut Mohamed El-Sherbiny verfasserin aut Mohammed Ahmad Al Mohaini verfasserin aut Krishnaraju Venkatesan verfasserin aut Yahya Bin Abdullah Alrashdi verfasserin aut Mamdouh Basheir Eldesoqui verfasserin aut Adel Ehab Ibrahim verfasserin aut Amal Fahmy Dawood verfasserin aut Ateya Megahed Ibrahim verfasserin aut Sami El Deeb verfasserin aut In Cancer Nanotechnology BMC, 2017 15(2024), 1, Seite 17 (DE-627)626052327 (DE-600)2553049-5 18686966 nnns volume:15 year:2024 number:1 pages:17 https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/article/9732a9626ac242ee9a27e064db1c6b29 kostenfrei https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/toc/1868-6958 Journal toc kostenfrei https://doaj.org/toc/1868-6966 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 17
allfields_unstemmed	10.1186/s12645-024-00249-3 doi (DE-627)DOAJ092188664 (DE-599)DOAJ9732a9626ac242ee9a27e064db1c6b29 DE-627 ger DE-627 rakwb eng RC254-282 Jamal Moideen Muthu Mohamed verfasserin aut Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC. Ultrasonication Quercetin MTT assay Box–Behnken design Anticancer Solid lipid nanoparticles Neoplasms. Tumors. Oncology. Including cancer and carcinogens Fazil Ahmad verfasserin aut Mohamed El-Sherbiny verfasserin aut Mohammed Ahmad Al Mohaini verfasserin aut Krishnaraju Venkatesan verfasserin aut Yahya Bin Abdullah Alrashdi verfasserin aut Mamdouh Basheir Eldesoqui verfasserin aut Adel Ehab Ibrahim verfasserin aut Amal Fahmy Dawood verfasserin aut Ateya Megahed Ibrahim verfasserin aut Sami El Deeb verfasserin aut In Cancer Nanotechnology BMC, 2017 15(2024), 1, Seite 17 (DE-627)626052327 (DE-600)2553049-5 18686966 nnns volume:15 year:2024 number:1 pages:17 https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/article/9732a9626ac242ee9a27e064db1c6b29 kostenfrei https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/toc/1868-6958 Journal toc kostenfrei https://doaj.org/toc/1868-6966 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 17
allfieldsGer	10.1186/s12645-024-00249-3 doi (DE-627)DOAJ092188664 (DE-599)DOAJ9732a9626ac242ee9a27e064db1c6b29 DE-627 ger DE-627 rakwb eng RC254-282 Jamal Moideen Muthu Mohamed verfasserin aut Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC. Ultrasonication Quercetin MTT assay Box–Behnken design Anticancer Solid lipid nanoparticles Neoplasms. Tumors. Oncology. Including cancer and carcinogens Fazil Ahmad verfasserin aut Mohamed El-Sherbiny verfasserin aut Mohammed Ahmad Al Mohaini verfasserin aut Krishnaraju Venkatesan verfasserin aut Yahya Bin Abdullah Alrashdi verfasserin aut Mamdouh Basheir Eldesoqui verfasserin aut Adel Ehab Ibrahim verfasserin aut Amal Fahmy Dawood verfasserin aut Ateya Megahed Ibrahim verfasserin aut Sami El Deeb verfasserin aut In Cancer Nanotechnology BMC, 2017 15(2024), 1, Seite 17 (DE-627)626052327 (DE-600)2553049-5 18686966 nnns volume:15 year:2024 number:1 pages:17 https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/article/9732a9626ac242ee9a27e064db1c6b29 kostenfrei https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/toc/1868-6958 Journal toc kostenfrei https://doaj.org/toc/1868-6966 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 17
allfieldsSound	10.1186/s12645-024-00249-3 doi (DE-627)DOAJ092188664 (DE-599)DOAJ9732a9626ac242ee9a27e064db1c6b29 DE-627 ger DE-627 rakwb eng RC254-282 Jamal Moideen Muthu Mohamed verfasserin aut Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC. Ultrasonication Quercetin MTT assay Box–Behnken design Anticancer Solid lipid nanoparticles Neoplasms. Tumors. Oncology. Including cancer and carcinogens Fazil Ahmad verfasserin aut Mohamed El-Sherbiny verfasserin aut Mohammed Ahmad Al Mohaini verfasserin aut Krishnaraju Venkatesan verfasserin aut Yahya Bin Abdullah Alrashdi verfasserin aut Mamdouh Basheir Eldesoqui verfasserin aut Adel Ehab Ibrahim verfasserin aut Amal Fahmy Dawood verfasserin aut Ateya Megahed Ibrahim verfasserin aut Sami El Deeb verfasserin aut In Cancer Nanotechnology BMC, 2017 15(2024), 1, Seite 17 (DE-627)626052327 (DE-600)2553049-5 18686966 nnns volume:15 year:2024 number:1 pages:17 https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/article/9732a9626ac242ee9a27e064db1c6b29 kostenfrei https://doi.org/10.1186/s12645-024-00249-3 kostenfrei https://doaj.org/toc/1868-6958 Journal toc kostenfrei https://doaj.org/toc/1868-6966 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 1 17
language	English
source	In Cancer Nanotechnology 15(2024), 1, Seite 17 volume:15 year:2024 number:1 pages:17
sourceStr	In Cancer Nanotechnology 15(2024), 1, Seite 17 volume:15 year:2024 number:1 pages:17
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Ultrasonication Quercetin MTT assay Box–Behnken design Anticancer Solid lipid nanoparticles Neoplasms. Tumors. Oncology. Including cancer and carcinogens
isfreeaccess_bool	true
container_title	Cancer Nanotechnology
authorswithroles_txt_mv	Jamal Moideen Muthu Mohamed @@aut@@ Fazil Ahmad @@aut@@ Mohamed El-Sherbiny @@aut@@ Mohammed Ahmad Al Mohaini @@aut@@ Krishnaraju Venkatesan @@aut@@ Yahya Bin Abdullah Alrashdi @@aut@@ Mamdouh Basheir Eldesoqui @@aut@@ Adel Ehab Ibrahim @@aut@@ Amal Fahmy Dawood @@aut@@ Ateya Megahed Ibrahim @@aut@@ Sami El Deeb @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	626052327
id	DOAJ092188664
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ092188664</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414093942.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">240412s2024 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s12645-024-00249-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ092188664</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ9732a9626ac242ee9a27e064db1c6b29</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC254-282</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Jamal Moideen Muthu Mohamed</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2024</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ultrasonication</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Quercetin</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">MTT assay</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Box–Behnken design</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Anticancer</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Solid lipid nanoparticles</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Neoplasms. Tumors. Oncology. 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callnumber-first	R - Medicine
author	Jamal Moideen Muthu Mohamed
spellingShingle	Jamal Moideen Muthu Mohamed misc RC254-282 misc Ultrasonication misc Quercetin misc MTT assay misc Box–Behnken design misc Anticancer misc Solid lipid nanoparticles misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer
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topic_title	RC254-282 Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer Ultrasonication Quercetin MTT assay Box–Behnken design Anticancer Solid lipid nanoparticles
topic	misc RC254-282 misc Ultrasonication misc Quercetin misc MTT assay misc Box–Behnken design misc Anticancer misc Solid lipid nanoparticles misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc Ultrasonication misc Quercetin misc MTT assay misc Box–Behnken design misc Anticancer misc Solid lipid nanoparticles misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer
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title_full	Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer
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author_browse	Jamal Moideen Muthu Mohamed Fazil Ahmad Mohamed El-Sherbiny Mohammed Ahmad Al Mohaini Krishnaraju Venkatesan Yahya Bin Abdullah Alrashdi Mamdouh Basheir Eldesoqui Adel Ehab Ibrahim Amal Fahmy Dawood Ateya Megahed Ibrahim Sami El Deeb
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title_sort	optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer
callnumber	RC254-282
title_auth	Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer
abstract	Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC.
abstractGer	Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC.
abstract_unstemmed	Abstract Background Colorectal cancer (CRC) is a type of cancer that affects the colon or rectum and occurs in individuals over the age of 50, although it can affect people of all ages. Quercetin is a flavonoid, which is a type of plant pigment with antioxidant and anti-inflammatory properties. Some studies have explored the potential of quercetin as an adjuvant therapy to enhance the effectiveness of chemotherapy or radiation therapy. Methodology In the proposed work, the nano-biomaterials of solid lipids such as stearic acid (SA) and tripalmitin (TpN) as well as the surfactants tween 80 and span 80 were used to prepare novel quercetin (QuR)-loaded-solid lipid nanoparticles (QuR-SLNs) for medical applications in colorectal cancer (CRC). The resulting bio-nano SLNs’ mean entrapment efficiency (EE) and particle size (PS) were optimized by Box–Behnken design (BBD) approach based on the response-like surface methodology (RSM). The variables include lipid ratio (X 1), surfactant ratio (X 2), QuR-to-lipid ratio (X 3), the sonication time (X 4), and the homogenization time (X 5). Requirements on the maximum EE (%) and minimum PS (nm) were optimized for the preparation of QuR-SLN. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) were then used to analyze the optimized SLN and to find the crystalline state of QuR with lipid relationship. In addition, on the Caco-2 cells, at IC50 (49 µM/mL), in vitro cytotoxicity was attained. Results The optimized QuR-SLN had practically spherical shapes, with % EE and a PS of 97.8 ± 1.16% and 132.16 ± 4.1 nm, respectively. In aqueous media, the degree of lipid crystallinity and the lipid modification was investigated, and the QuR incorporation and release patterns showed high correlations with both. The results showed that over 41.12 ± 1.6% of the bio-nano QuR-SLNs was released gradually over the course of 48 h, demonstrating effective QuR delayed release. Results on apoptotic observations indicate that apoptosis accounts for the majority of cell death, while necrosis, a type of cell death, constitutes a very minor portion. In conclusion, the prepared bio-nano QuR-SLNs might improve cytotoxicity and can act as an ideal carrier for the delivery of QuR and this preparation is used in the treatment of CRC.
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title_short	Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer
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Optimization and characterization of quercetin-loaded solid lipid nanoparticles for biomedical application in colorectal cancer

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