Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma

Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implem...
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Autor*in:	Ewelina Kowal-Wisniewska [verfasserIn] Katarzyna Jaskiewicz [verfasserIn] Anna Bartochowska [verfasserIn] Katarzyna Kiwerska [verfasserIn] Adam Ustaszewski [verfasserIn] Tomasz Gorecki [verfasserIn] Maciej Giefing [verfasserIn] Jaroslaw Paluszczak [verfasserIn] Malgorzata Wierzbicka [verfasserIn] Malgorzata Jarmuz-Szymczak [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Übergeordnetes Werk:	In: Scientific Reports - Nature Portfolio, 2011, 14(2024), 1, Seite 11
Übergeordnetes Werk:	volume:14 ; year:2024 ; number:1 ; pages:11

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1038/s41598-024-52031-5

Katalog-ID:	DOAJ092223192

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allfields	10.1038/s41598-024-52031-5 doi (DE-627)DOAJ092223192 (DE-599)DOAJ84eb623be8e04088acb80ed44762f2f1 DE-627 ger DE-627 rakwb eng Ewelina Kowal-Wisniewska verfasserin aut Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p < 0.0001) and a time dependent decrease of cfDNA concentration after tumor resection. Our results also revealed a significant increase of cfDNA concentration with age in both, healthy volunteers (p = 0.04) and HNSCC patients (p = 0.000002). Moreover, considering the multitude of HNSCC locations, we showed the lack of difference in cfDNA concentration depending on the anatomical location. Furthermore, we demonstrated a trend toward higher cfDNA length (range 35–10380 and 500–10380 bp) in the group of patients with recurrence during follow-up. In conclusion, our study provide a broad characterization of cfDNA fractions in HNSCC patients and healthy controls. These findings point to several aspects necessary to consider when implementing liquid biopsy in clinical practice including: (I) time required for epithelial regeneration to avoid falsely elevated levels of cfDNA not resulting from active cancer, (II) age-related accumulation of nucleic acids accompanied by less efficient elimination of cfDNA and (III) higher cfDNA length in patients with recurrence during follow-up, reflecting predominance of tumor necrosis. Medicine R Science Q Katarzyna Jaskiewicz verfasserin aut Anna Bartochowska verfasserin aut Katarzyna Kiwerska verfasserin aut Adam Ustaszewski verfasserin aut Tomasz Gorecki verfasserin aut Maciej Giefing verfasserin aut Jaroslaw Paluszczak verfasserin aut Malgorzata Wierzbicka verfasserin aut Malgorzata Jarmuz-Szymczak verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 11 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:11 https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/article/84eb623be8e04088acb80ed44762f2f1 kostenfrei https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 11
spelling	10.1038/s41598-024-52031-5 doi (DE-627)DOAJ092223192 (DE-599)DOAJ84eb623be8e04088acb80ed44762f2f1 DE-627 ger DE-627 rakwb eng Ewelina Kowal-Wisniewska verfasserin aut Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p < 0.0001) and a time dependent decrease of cfDNA concentration after tumor resection. Our results also revealed a significant increase of cfDNA concentration with age in both, healthy volunteers (p = 0.04) and HNSCC patients (p = 0.000002). Moreover, considering the multitude of HNSCC locations, we showed the lack of difference in cfDNA concentration depending on the anatomical location. Furthermore, we demonstrated a trend toward higher cfDNA length (range 35–10380 and 500–10380 bp) in the group of patients with recurrence during follow-up. In conclusion, our study provide a broad characterization of cfDNA fractions in HNSCC patients and healthy controls. These findings point to several aspects necessary to consider when implementing liquid biopsy in clinical practice including: (I) time required for epithelial regeneration to avoid falsely elevated levels of cfDNA not resulting from active cancer, (II) age-related accumulation of nucleic acids accompanied by less efficient elimination of cfDNA and (III) higher cfDNA length in patients with recurrence during follow-up, reflecting predominance of tumor necrosis. Medicine R Science Q Katarzyna Jaskiewicz verfasserin aut Anna Bartochowska verfasserin aut Katarzyna Kiwerska verfasserin aut Adam Ustaszewski verfasserin aut Tomasz Gorecki verfasserin aut Maciej Giefing verfasserin aut Jaroslaw Paluszczak verfasserin aut Malgorzata Wierzbicka verfasserin aut Malgorzata Jarmuz-Szymczak verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 11 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:11 https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/article/84eb623be8e04088acb80ed44762f2f1 kostenfrei https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 11
allfields_unstemmed	10.1038/s41598-024-52031-5 doi (DE-627)DOAJ092223192 (DE-599)DOAJ84eb623be8e04088acb80ed44762f2f1 DE-627 ger DE-627 rakwb eng Ewelina Kowal-Wisniewska verfasserin aut Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p < 0.0001) and a time dependent decrease of cfDNA concentration after tumor resection. Our results also revealed a significant increase of cfDNA concentration with age in both, healthy volunteers (p = 0.04) and HNSCC patients (p = 0.000002). Moreover, considering the multitude of HNSCC locations, we showed the lack of difference in cfDNA concentration depending on the anatomical location. Furthermore, we demonstrated a trend toward higher cfDNA length (range 35–10380 and 500–10380 bp) in the group of patients with recurrence during follow-up. In conclusion, our study provide a broad characterization of cfDNA fractions in HNSCC patients and healthy controls. These findings point to several aspects necessary to consider when implementing liquid biopsy in clinical practice including: (I) time required for epithelial regeneration to avoid falsely elevated levels of cfDNA not resulting from active cancer, (II) age-related accumulation of nucleic acids accompanied by less efficient elimination of cfDNA and (III) higher cfDNA length in patients with recurrence during follow-up, reflecting predominance of tumor necrosis. Medicine R Science Q Katarzyna Jaskiewicz verfasserin aut Anna Bartochowska verfasserin aut Katarzyna Kiwerska verfasserin aut Adam Ustaszewski verfasserin aut Tomasz Gorecki verfasserin aut Maciej Giefing verfasserin aut Jaroslaw Paluszczak verfasserin aut Malgorzata Wierzbicka verfasserin aut Malgorzata Jarmuz-Szymczak verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 11 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:11 https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/article/84eb623be8e04088acb80ed44762f2f1 kostenfrei https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 11
allfieldsGer	10.1038/s41598-024-52031-5 doi (DE-627)DOAJ092223192 (DE-599)DOAJ84eb623be8e04088acb80ed44762f2f1 DE-627 ger DE-627 rakwb eng Ewelina Kowal-Wisniewska verfasserin aut Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p < 0.0001) and a time dependent decrease of cfDNA concentration after tumor resection. Our results also revealed a significant increase of cfDNA concentration with age in both, healthy volunteers (p = 0.04) and HNSCC patients (p = 0.000002). Moreover, considering the multitude of HNSCC locations, we showed the lack of difference in cfDNA concentration depending on the anatomical location. Furthermore, we demonstrated a trend toward higher cfDNA length (range 35–10380 and 500–10380 bp) in the group of patients with recurrence during follow-up. In conclusion, our study provide a broad characterization of cfDNA fractions in HNSCC patients and healthy controls. These findings point to several aspects necessary to consider when implementing liquid biopsy in clinical practice including: (I) time required for epithelial regeneration to avoid falsely elevated levels of cfDNA not resulting from active cancer, (II) age-related accumulation of nucleic acids accompanied by less efficient elimination of cfDNA and (III) higher cfDNA length in patients with recurrence during follow-up, reflecting predominance of tumor necrosis. Medicine R Science Q Katarzyna Jaskiewicz verfasserin aut Anna Bartochowska verfasserin aut Katarzyna Kiwerska verfasserin aut Adam Ustaszewski verfasserin aut Tomasz Gorecki verfasserin aut Maciej Giefing verfasserin aut Jaroslaw Paluszczak verfasserin aut Malgorzata Wierzbicka verfasserin aut Malgorzata Jarmuz-Szymczak verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 11 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:11 https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/article/84eb623be8e04088acb80ed44762f2f1 kostenfrei https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 11
allfieldsSound	10.1038/s41598-024-52031-5 doi (DE-627)DOAJ092223192 (DE-599)DOAJ84eb623be8e04088acb80ed44762f2f1 DE-627 ger DE-627 rakwb eng Ewelina Kowal-Wisniewska verfasserin aut Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p < 0.0001) and a time dependent decrease of cfDNA concentration after tumor resection. Our results also revealed a significant increase of cfDNA concentration with age in both, healthy volunteers (p = 0.04) and HNSCC patients (p = 0.000002). Moreover, considering the multitude of HNSCC locations, we showed the lack of difference in cfDNA concentration depending on the anatomical location. Furthermore, we demonstrated a trend toward higher cfDNA length (range 35–10380 and 500–10380 bp) in the group of patients with recurrence during follow-up. In conclusion, our study provide a broad characterization of cfDNA fractions in HNSCC patients and healthy controls. These findings point to several aspects necessary to consider when implementing liquid biopsy in clinical practice including: (I) time required for epithelial regeneration to avoid falsely elevated levels of cfDNA not resulting from active cancer, (II) age-related accumulation of nucleic acids accompanied by less efficient elimination of cfDNA and (III) higher cfDNA length in patients with recurrence during follow-up, reflecting predominance of tumor necrosis. Medicine R Science Q Katarzyna Jaskiewicz verfasserin aut Anna Bartochowska verfasserin aut Katarzyna Kiwerska verfasserin aut Adam Ustaszewski verfasserin aut Tomasz Gorecki verfasserin aut Maciej Giefing verfasserin aut Jaroslaw Paluszczak verfasserin aut Malgorzata Wierzbicka verfasserin aut Malgorzata Jarmuz-Szymczak verfasserin aut In Scientific Reports Nature Portfolio, 2011 14(2024), 1, Seite 11 (DE-627)663366712 (DE-600)2615211-3 20452322 nnns volume:14 year:2024 number:1 pages:11 https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/article/84eb623be8e04088acb80ed44762f2f1 kostenfrei https://doi.org/10.1038/s41598-024-52031-5 kostenfrei https://doaj.org/toc/2045-2322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 1 11
language	English
source	In Scientific Reports 14(2024), 1, Seite 11 volume:14 year:2024 number:1 pages:11
sourceStr	In Scientific Reports 14(2024), 1, Seite 11 volume:14 year:2024 number:1 pages:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Medicine R Science Q
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container_title	Scientific Reports
authorswithroles_txt_mv	Ewelina Kowal-Wisniewska @@aut@@ Katarzyna Jaskiewicz @@aut@@ Anna Bartochowska @@aut@@ Katarzyna Kiwerska @@aut@@ Adam Ustaszewski @@aut@@ Tomasz Gorecki @@aut@@ Maciej Giefing @@aut@@ Jaroslaw Paluszczak @@aut@@ Malgorzata Wierzbicka @@aut@@ Malgorzata Jarmuz-Szymczak @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	663366712
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author	Ewelina Kowal-Wisniewska
spellingShingle	Ewelina Kowal-Wisniewska misc Medicine misc R misc Science misc Q Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma
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topic_title	Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma
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title	Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma
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title_full	Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma
author_sort	Ewelina Kowal-Wisniewska
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author_browse	Ewelina Kowal-Wisniewska Katarzyna Jaskiewicz Anna Bartochowska Katarzyna Kiwerska Adam Ustaszewski Tomasz Gorecki Maciej Giefing Jaroslaw Paluszczak Malgorzata Wierzbicka Malgorzata Jarmuz-Szymczak
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title_sort	towards effectiveness of cell free dna based liquid biopsy in head and neck squamous cell carcinoma
title_auth	Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma
abstract	Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p < 0.0001) and a time dependent decrease of cfDNA concentration after tumor resection. Our results also revealed a significant increase of cfDNA concentration with age in both, healthy volunteers (p = 0.04) and HNSCC patients (p = 0.000002). Moreover, considering the multitude of HNSCC locations, we showed the lack of difference in cfDNA concentration depending on the anatomical location. Furthermore, we demonstrated a trend toward higher cfDNA length (range 35–10380 and 500–10380 bp) in the group of patients with recurrence during follow-up. In conclusion, our study provide a broad characterization of cfDNA fractions in HNSCC patients and healthy controls. These findings point to several aspects necessary to consider when implementing liquid biopsy in clinical practice including: (I) time required for epithelial regeneration to avoid falsely elevated levels of cfDNA not resulting from active cancer, (II) age-related accumulation of nucleic acids accompanied by less efficient elimination of cfDNA and (III) higher cfDNA length in patients with recurrence during follow-up, reflecting predominance of tumor necrosis.
abstractGer	Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p < 0.0001) and a time dependent decrease of cfDNA concentration after tumor resection. Our results also revealed a significant increase of cfDNA concentration with age in both, healthy volunteers (p = 0.04) and HNSCC patients (p = 0.000002). Moreover, considering the multitude of HNSCC locations, we showed the lack of difference in cfDNA concentration depending on the anatomical location. Furthermore, we demonstrated a trend toward higher cfDNA length (range 35–10380 and 500–10380 bp) in the group of patients with recurrence during follow-up. In conclusion, our study provide a broad characterization of cfDNA fractions in HNSCC patients and healthy controls. These findings point to several aspects necessary to consider when implementing liquid biopsy in clinical practice including: (I) time required for epithelial regeneration to avoid falsely elevated levels of cfDNA not resulting from active cancer, (II) age-related accumulation of nucleic acids accompanied by less efficient elimination of cfDNA and (III) higher cfDNA length in patients with recurrence during follow-up, reflecting predominance of tumor necrosis.
abstract_unstemmed	Abstract Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p < 0.0001) and a time dependent decrease of cfDNA concentration after tumor resection. Our results also revealed a significant increase of cfDNA concentration with age in both, healthy volunteers (p = 0.04) and HNSCC patients (p = 0.000002). Moreover, considering the multitude of HNSCC locations, we showed the lack of difference in cfDNA concentration depending on the anatomical location. Furthermore, we demonstrated a trend toward higher cfDNA length (range 35–10380 and 500–10380 bp) in the group of patients with recurrence during follow-up. In conclusion, our study provide a broad characterization of cfDNA fractions in HNSCC patients and healthy controls. These findings point to several aspects necessary to consider when implementing liquid biopsy in clinical practice including: (I) time required for epithelial regeneration to avoid falsely elevated levels of cfDNA not resulting from active cancer, (II) age-related accumulation of nucleic acids accompanied by less efficient elimination of cfDNA and (III) higher cfDNA length in patients with recurrence during follow-up, reflecting predominance of tumor necrosis.
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title_short	Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma
url	https://doi.org/10.1038/s41598-024-52031-5 https://doaj.org/article/84eb623be8e04088acb80ed44762f2f1 https://doaj.org/toc/2045-2322
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