Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction

ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of clea...
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Autor*in:	Siu-Ying Lau [verfasserIn] Pui Wang [verfasserIn] Bobo Wing-Yee Mok [verfasserIn] Anna Jinxia Zhang [verfasserIn] Hin Chu [verfasserIn] Andrew Chak-Yiu Lee [verfasserIn] Shaofeng Deng [verfasserIn] Pin Chen [verfasserIn] Kwok-Hung Chan [verfasserIn] Wenjun Song [verfasserIn] Zhiwei Chen [verfasserIn] Kelvin Kai-Wang To [verfasserIn] Jasper Fuk-Woo Chan [verfasserIn] Kwok-Yung Yuen [verfasserIn] Honglin Chen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Coronavirus COVID-19 SARS-CoV-2 Spike mutant Spike S1/S2 mutant

Übergeordnetes Werk:	In: Emerging Microbes and Infections - Taylor & Francis Group, 2013, 9(2020), 1, Seite 837-842
Übergeordnetes Werk:	volume:9 ; year:2020 ; number:1 ; pages:837-842

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1080/22221751.2020.1756700

Katalog-ID:	DOAJ092269443

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allfields	10.1080/22221751.2020.1756700 doi (DE-627)DOAJ092269443 (DE-599)DOAJ5c83dd9cd93c484394f5312b81bd84f2 DE-627 ger DE-627 rakwb eng RC109-216 QR1-502 Siu-Ying Lau verfasserin aut Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated. Coronavirus COVID-19 SARS-CoV-2 Spike mutant Spike S1/S2 mutant Infectious and parasitic diseases Microbiology Pui Wang verfasserin aut Bobo Wing-Yee Mok verfasserin aut Anna Jinxia Zhang verfasserin aut Hin Chu verfasserin aut Andrew Chak-Yiu Lee verfasserin aut Shaofeng Deng verfasserin aut Pin Chen verfasserin aut Kwok-Hung Chan verfasserin aut Wenjun Song verfasserin aut Zhiwei Chen verfasserin aut Kelvin Kai-Wang To verfasserin aut Jasper Fuk-Woo Chan verfasserin aut Kwok-Yung Yuen verfasserin aut Honglin Chen verfasserin aut In Emerging Microbes and Infections Taylor & Francis Group, 2013 9(2020), 1, Seite 837-842 (DE-627)726120715 (DE-600)2681359-2 22221751 nnns volume:9 year:2020 number:1 pages:837-842 https://doi.org/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/article/5c83dd9cd93c484394f5312b81bd84f2 kostenfrei https://www.tandfonline.com/doi/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/toc/2222-1751 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 1 837-842
spelling	10.1080/22221751.2020.1756700 doi (DE-627)DOAJ092269443 (DE-599)DOAJ5c83dd9cd93c484394f5312b81bd84f2 DE-627 ger DE-627 rakwb eng RC109-216 QR1-502 Siu-Ying Lau verfasserin aut Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated. Coronavirus COVID-19 SARS-CoV-2 Spike mutant Spike S1/S2 mutant Infectious and parasitic diseases Microbiology Pui Wang verfasserin aut Bobo Wing-Yee Mok verfasserin aut Anna Jinxia Zhang verfasserin aut Hin Chu verfasserin aut Andrew Chak-Yiu Lee verfasserin aut Shaofeng Deng verfasserin aut Pin Chen verfasserin aut Kwok-Hung Chan verfasserin aut Wenjun Song verfasserin aut Zhiwei Chen verfasserin aut Kelvin Kai-Wang To verfasserin aut Jasper Fuk-Woo Chan verfasserin aut Kwok-Yung Yuen verfasserin aut Honglin Chen verfasserin aut In Emerging Microbes and Infections Taylor & Francis Group, 2013 9(2020), 1, Seite 837-842 (DE-627)726120715 (DE-600)2681359-2 22221751 nnns volume:9 year:2020 number:1 pages:837-842 https://doi.org/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/article/5c83dd9cd93c484394f5312b81bd84f2 kostenfrei https://www.tandfonline.com/doi/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/toc/2222-1751 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 1 837-842
allfields_unstemmed	10.1080/22221751.2020.1756700 doi (DE-627)DOAJ092269443 (DE-599)DOAJ5c83dd9cd93c484394f5312b81bd84f2 DE-627 ger DE-627 rakwb eng RC109-216 QR1-502 Siu-Ying Lau verfasserin aut Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated. Coronavirus COVID-19 SARS-CoV-2 Spike mutant Spike S1/S2 mutant Infectious and parasitic diseases Microbiology Pui Wang verfasserin aut Bobo Wing-Yee Mok verfasserin aut Anna Jinxia Zhang verfasserin aut Hin Chu verfasserin aut Andrew Chak-Yiu Lee verfasserin aut Shaofeng Deng verfasserin aut Pin Chen verfasserin aut Kwok-Hung Chan verfasserin aut Wenjun Song verfasserin aut Zhiwei Chen verfasserin aut Kelvin Kai-Wang To verfasserin aut Jasper Fuk-Woo Chan verfasserin aut Kwok-Yung Yuen verfasserin aut Honglin Chen verfasserin aut In Emerging Microbes and Infections Taylor & Francis Group, 2013 9(2020), 1, Seite 837-842 (DE-627)726120715 (DE-600)2681359-2 22221751 nnns volume:9 year:2020 number:1 pages:837-842 https://doi.org/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/article/5c83dd9cd93c484394f5312b81bd84f2 kostenfrei https://www.tandfonline.com/doi/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/toc/2222-1751 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 1 837-842
allfieldsGer	10.1080/22221751.2020.1756700 doi (DE-627)DOAJ092269443 (DE-599)DOAJ5c83dd9cd93c484394f5312b81bd84f2 DE-627 ger DE-627 rakwb eng RC109-216 QR1-502 Siu-Ying Lau verfasserin aut Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated. Coronavirus COVID-19 SARS-CoV-2 Spike mutant Spike S1/S2 mutant Infectious and parasitic diseases Microbiology Pui Wang verfasserin aut Bobo Wing-Yee Mok verfasserin aut Anna Jinxia Zhang verfasserin aut Hin Chu verfasserin aut Andrew Chak-Yiu Lee verfasserin aut Shaofeng Deng verfasserin aut Pin Chen verfasserin aut Kwok-Hung Chan verfasserin aut Wenjun Song verfasserin aut Zhiwei Chen verfasserin aut Kelvin Kai-Wang To verfasserin aut Jasper Fuk-Woo Chan verfasserin aut Kwok-Yung Yuen verfasserin aut Honglin Chen verfasserin aut In Emerging Microbes and Infections Taylor & Francis Group, 2013 9(2020), 1, Seite 837-842 (DE-627)726120715 (DE-600)2681359-2 22221751 nnns volume:9 year:2020 number:1 pages:837-842 https://doi.org/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/article/5c83dd9cd93c484394f5312b81bd84f2 kostenfrei https://www.tandfonline.com/doi/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/toc/2222-1751 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 1 837-842
allfieldsSound	10.1080/22221751.2020.1756700 doi (DE-627)DOAJ092269443 (DE-599)DOAJ5c83dd9cd93c484394f5312b81bd84f2 DE-627 ger DE-627 rakwb eng RC109-216 QR1-502 Siu-Ying Lau verfasserin aut Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated. Coronavirus COVID-19 SARS-CoV-2 Spike mutant Spike S1/S2 mutant Infectious and parasitic diseases Microbiology Pui Wang verfasserin aut Bobo Wing-Yee Mok verfasserin aut Anna Jinxia Zhang verfasserin aut Hin Chu verfasserin aut Andrew Chak-Yiu Lee verfasserin aut Shaofeng Deng verfasserin aut Pin Chen verfasserin aut Kwok-Hung Chan verfasserin aut Wenjun Song verfasserin aut Zhiwei Chen verfasserin aut Kelvin Kai-Wang To verfasserin aut Jasper Fuk-Woo Chan verfasserin aut Kwok-Yung Yuen verfasserin aut Honglin Chen verfasserin aut In Emerging Microbes and Infections Taylor & Francis Group, 2013 9(2020), 1, Seite 837-842 (DE-627)726120715 (DE-600)2681359-2 22221751 nnns volume:9 year:2020 number:1 pages:837-842 https://doi.org/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/article/5c83dd9cd93c484394f5312b81bd84f2 kostenfrei https://www.tandfonline.com/doi/10.1080/22221751.2020.1756700 kostenfrei https://doaj.org/toc/2222-1751 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 1 837-842
language	English
source	In Emerging Microbes and Infections 9(2020), 1, Seite 837-842 volume:9 year:2020 number:1 pages:837-842
sourceStr	In Emerging Microbes and Infections 9(2020), 1, Seite 837-842 volume:9 year:2020 number:1 pages:837-842
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Coronavirus COVID-19 SARS-CoV-2 Spike mutant Spike S1/S2 mutant Infectious and parasitic diseases Microbiology
isfreeaccess_bool	true
container_title	Emerging Microbes and Infections
authorswithroles_txt_mv	Siu-Ying Lau @@aut@@ Pui Wang @@aut@@ Bobo Wing-Yee Mok @@aut@@ Anna Jinxia Zhang @@aut@@ Hin Chu @@aut@@ Andrew Chak-Yiu Lee @@aut@@ Shaofeng Deng @@aut@@ Pin Chen @@aut@@ Kwok-Hung Chan @@aut@@ Wenjun Song @@aut@@ Zhiwei Chen @@aut@@ Kelvin Kai-Wang To @@aut@@ Jasper Fuk-Woo Chan @@aut@@ Kwok-Yung Yuen @@aut@@ Honglin Chen @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	726120715
id	DOAJ092269443
language_de	englisch
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callnumber-first	R - Medicine
author	Siu-Ying Lau
spellingShingle	Siu-Ying Lau misc RC109-216 misc QR1-502 misc Coronavirus misc COVID-19 misc SARS-CoV-2 misc Spike mutant misc Spike S1/S2 mutant misc Infectious and parasitic diseases misc Microbiology Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
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topic_title	RC109-216 QR1-502 Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction Coronavirus COVID-19 SARS-CoV-2 Spike mutant Spike S1/S2 mutant
topic	misc RC109-216 misc QR1-502 misc Coronavirus misc COVID-19 misc SARS-CoV-2 misc Spike mutant misc Spike S1/S2 mutant misc Infectious and parasitic diseases misc Microbiology
topic_unstemmed	misc RC109-216 misc QR1-502 misc Coronavirus misc COVID-19 misc SARS-CoV-2 misc Spike mutant misc Spike S1/S2 mutant misc Infectious and parasitic diseases misc Microbiology
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title	Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
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title_full	Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
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journal	Emerging Microbes and Infections
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author_browse	Siu-Ying Lau Pui Wang Bobo Wing-Yee Mok Anna Jinxia Zhang Hin Chu Andrew Chak-Yiu Lee Shaofeng Deng Pin Chen Kwok-Hung Chan Wenjun Song Zhiwei Chen Kelvin Kai-Wang To Jasper Fuk-Woo Chan Kwok-Yung Yuen Honglin Chen
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title_sort	attenuated sars-cov-2 variants with deletions at the s1/s2 junction
callnumber	RC109-216
title_auth	Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
abstract	ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated.
abstractGer	ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated.
abstract_unstemmed	ABSTRACTThe emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated.
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title_short	Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
url	https://doi.org/10.1080/22221751.2020.1756700 https://doaj.org/article/5c83dd9cd93c484394f5312b81bd84f2 https://www.tandfonline.com/doi/10.1080/22221751.2020.1756700 https://doaj.org/toc/2222-1751
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author2	Pui Wang Bobo Wing-Yee Mok Anna Jinxia Zhang Hin Chu Andrew Chak-Yiu Lee Shaofeng Deng Pin Chen Kwok-Hung Chan Wenjun Song Zhiwei Chen Kelvin Kai-Wang To Jasper Fuk-Woo Chan Kwok-Yung Yuen Honglin Chen
author2Str	Pui Wang Bobo Wing-Yee Mok Anna Jinxia Zhang Hin Chu Andrew Chak-Yiu Lee Shaofeng Deng Pin Chen Kwok-Hung Chan Wenjun Song Zhiwei Chen Kelvin Kai-Wang To Jasper Fuk-Woo Chan Kwok-Yung Yuen Honglin Chen
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doi_str	10.1080/22221751.2020.1756700
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up_date	2024-07-04T00:44:02.224Z
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