Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau

Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis developm...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Peipei Liu [verfasserIn] Ziniu Li [verfasserIn] Qiuyuan Zhang [verfasserIn] Jiao Qiao [verfasserIn] Chenjun Zheng [verfasserIn] Wenping Zheng [verfasserIn] Hongyu Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Zeugodacus tau Iso-Seq RNA-Seq testis development spermatogenesis vitamin metabolism

Übergeordnetes Werk:	In: Frontiers in Cell and Developmental Biology - Frontiers Media S.A., 2014, 12(2024)
Übergeordnetes Werk:	volume:12 ; year:2024

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fcell.2024.1356151

Katalog-ID:	DOAJ092281192

Internformat


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520			\|a Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species.
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allfields	10.3389/fcell.2024.1356151 doi (DE-627)DOAJ092281192 (DE-599)DOAJf629312ff9004fcf9e8facf63aaa0c2f DE-627 ger DE-627 rakwb eng QH301-705.5 Peipei Liu verfasserin aut Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species. Zeugodacus tau Iso-Seq RNA-Seq testis development spermatogenesis vitamin metabolism Biology (General) Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 12(2024) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:12 year:2024 https://doi.org/10.3389/fcell.2024.1356151 kostenfrei https://doaj.org/article/f629312ff9004fcf9e8facf63aaa0c2f kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2024.1356151/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024
spelling	10.3389/fcell.2024.1356151 doi (DE-627)DOAJ092281192 (DE-599)DOAJf629312ff9004fcf9e8facf63aaa0c2f DE-627 ger DE-627 rakwb eng QH301-705.5 Peipei Liu verfasserin aut Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species. Zeugodacus tau Iso-Seq RNA-Seq testis development spermatogenesis vitamin metabolism Biology (General) Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 12(2024) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:12 year:2024 https://doi.org/10.3389/fcell.2024.1356151 kostenfrei https://doaj.org/article/f629312ff9004fcf9e8facf63aaa0c2f kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2024.1356151/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024
allfields_unstemmed	10.3389/fcell.2024.1356151 doi (DE-627)DOAJ092281192 (DE-599)DOAJf629312ff9004fcf9e8facf63aaa0c2f DE-627 ger DE-627 rakwb eng QH301-705.5 Peipei Liu verfasserin aut Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species. Zeugodacus tau Iso-Seq RNA-Seq testis development spermatogenesis vitamin metabolism Biology (General) Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 12(2024) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:12 year:2024 https://doi.org/10.3389/fcell.2024.1356151 kostenfrei https://doaj.org/article/f629312ff9004fcf9e8facf63aaa0c2f kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2024.1356151/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024
allfieldsGer	10.3389/fcell.2024.1356151 doi (DE-627)DOAJ092281192 (DE-599)DOAJf629312ff9004fcf9e8facf63aaa0c2f DE-627 ger DE-627 rakwb eng QH301-705.5 Peipei Liu verfasserin aut Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species. Zeugodacus tau Iso-Seq RNA-Seq testis development spermatogenesis vitamin metabolism Biology (General) Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 12(2024) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:12 year:2024 https://doi.org/10.3389/fcell.2024.1356151 kostenfrei https://doaj.org/article/f629312ff9004fcf9e8facf63aaa0c2f kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2024.1356151/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024
allfieldsSound	10.3389/fcell.2024.1356151 doi (DE-627)DOAJ092281192 (DE-599)DOAJf629312ff9004fcf9e8facf63aaa0c2f DE-627 ger DE-627 rakwb eng QH301-705.5 Peipei Liu verfasserin aut Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species. Zeugodacus tau Iso-Seq RNA-Seq testis development spermatogenesis vitamin metabolism Biology (General) Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Peipei Liu verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Ziniu Li verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Qiuyuan Zhang verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Jiao Qiao verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Chenjun Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Wenping Zheng verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut Hongyu Zhang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 12(2024) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:12 year:2024 https://doi.org/10.3389/fcell.2024.1356151 kostenfrei https://doaj.org/article/f629312ff9004fcf9e8facf63aaa0c2f kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2024.1356151/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024
language	English
source	In Frontiers in Cell and Developmental Biology 12(2024) volume:12 year:2024
sourceStr	In Frontiers in Cell and Developmental Biology 12(2024) volume:12 year:2024
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Zeugodacus tau Iso-Seq RNA-Seq testis development spermatogenesis vitamin metabolism Biology (General)
isfreeaccess_bool	true
container_title	Frontiers in Cell and Developmental Biology
authorswithroles_txt_mv	Peipei Liu @@aut@@ Ziniu Li @@aut@@ Qiuyuan Zhang @@aut@@ Jiao Qiao @@aut@@ Chenjun Zheng @@aut@@ Wenping Zheng @@aut@@ Hongyu Zhang @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	770398138
id	DOAJ092281192
language_de	englisch
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An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. 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callnumber-first	Q - Science
author	Peipei Liu
spellingShingle	Peipei Liu misc QH301-705.5 misc Zeugodacus tau misc Iso-Seq misc RNA-Seq misc testis development misc spermatogenesis misc vitamin metabolism misc Biology (General) Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau
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topic_title	QH301-705.5 Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau Zeugodacus tau Iso-Seq RNA-Seq testis development spermatogenesis vitamin metabolism
topic	misc QH301-705.5 misc Zeugodacus tau misc Iso-Seq misc RNA-Seq misc testis development misc spermatogenesis misc vitamin metabolism misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc Zeugodacus tau misc Iso-Seq misc RNA-Seq misc testis development misc spermatogenesis misc vitamin metabolism misc Biology (General)
topic_browse	misc QH301-705.5 misc Zeugodacus tau misc Iso-Seq misc RNA-Seq misc testis development misc spermatogenesis misc vitamin metabolism misc Biology (General)
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title	Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau
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title_full	Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau
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author_browse	Peipei Liu Ziniu Li Qiuyuan Zhang Jiao Qiao Chenjun Zheng Wenping Zheng Hongyu Zhang
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title_sort	identification of testis development-related genes by combining iso-seq and rna-seq in zeugodacus tau
callnumber	QH301-705.5
title_auth	Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau
abstract	Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species.
abstractGer	Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species.
abstract_unstemmed	Introduction:Zeugodacus tau (Walker) is an invasive pest. An effective method to control this pest is the sterile insect technique (SIT). To better apply this technique, it is necessary to understand testis development progression.Methods: Differentially expressed genes (DEGs) during testis development were analyzed by PacBio Iso-Seq and RNA-seq.Results: RNA-Seq library of Z. tau testes on day 1, 6, and 11 post eclosion were constructed. We identified 755 and 865 differentially expressed genes in the comparisons of T6 (testes on day 6) vs. T1 and T11 vs. T1, respectively. The KEGG pathway analysis showed that the DEGs were significantly enriched in retinol metabolism, vitamin B6 metabolism, and ascorbate and aldarate metabolism pathways. Knockdown of retinol dehydrogenase 12-like (rdh12-like), pyridoxal kinase (pdxk) and regucalcin (rgn), the representative gene in each of the above 3 pathways, reduced the hatching rate of Z. tau offspring. In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. The findings provide an insight into testis development and spermatogenesis in tephritid species.
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title_short	Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau
url	https://doi.org/10.3389/fcell.2024.1356151 https://doaj.org/article/f629312ff9004fcf9e8facf63aaa0c2f https://www.frontiersin.org/articles/10.3389/fcell.2024.1356151/full https://doaj.org/toc/2296-634X
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author2	Peipei Liu Ziniu Li Qiuyuan Zhang Jiao Qiao Chenjun Zheng Wenping Zheng Hongyu Zhang
author2Str	Peipei Liu Ziniu Li Qiuyuan Zhang Jiao Qiao Chenjun Zheng Wenping Zheng Hongyu Zhang
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In addition, we identified 107 Drosophila spermatogenesis-related orthologous genes in Z. tau, of which innexin 2 (inx2) exhibited significantly up-regulated expression throughout testis development, and the knockdown of this gene reduced offspring hatching rate. Discussion: Our data indicated that rdh12-like, pdxk, rgn, and inx2 genes were related to testis development, and they were conserved in tephritid species. These results suggested that this gene might have the same function in tephritid. 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Identification of testis development-related genes by combining Iso-Seq and RNA-Seq in Zeugodacus tau

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