Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts
Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital...
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Deng Z [verfasserIn] Yu L [verfasserIn] Kuang Y [verfasserIn] Zhou Z [verfasserIn] Li X [verfasserIn] |
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Englisch |
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2024 |
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In: International Journal of Nanomedicine - Dove Medical Press, 2018, (2024), Seite 2469-2485 |
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year:2024 ; pages:2469-2485 |
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| 245 | 1 | 0 | |a Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts |
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| 520 | |a Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing | ||
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(DE-627)DOAJ092301177 (DE-599)DOAJfaaa18ebaa04464d9c8255ccf97f0ffd DE-627 ger DE-627 rakwb eng R5-920 Deng Z verfasserin aut Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing nanotubes gingival fibroblast cell behaviors cell adhesion gingival sealing Medicine (General) Yu L verfasserin aut Kuang Y verfasserin aut Zhou Z verfasserin aut Li X verfasserin aut In International Journal of Nanomedicine Dove Medical Press, 2018 (2024), Seite 2469-2485 (DE-627)537879560 (DE-600)2377464-2 11782013 nnns year:2024 pages:2469-2485 https://doaj.org/article/faaa18ebaa04464d9c8255ccf97f0ffd kostenfrei https://www.dovepress.com/highly-ordered-nanotube-like-microstructure-on-titanium-dental-implant-peer-reviewed-fulltext-article-IJN kostenfrei https://doaj.org/toc/1178-2013 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2024 2469-2485 |
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(DE-627)DOAJ092301177 (DE-599)DOAJfaaa18ebaa04464d9c8255ccf97f0ffd DE-627 ger DE-627 rakwb eng R5-920 Deng Z verfasserin aut Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing nanotubes gingival fibroblast cell behaviors cell adhesion gingival sealing Medicine (General) Yu L verfasserin aut Kuang Y verfasserin aut Zhou Z verfasserin aut Li X verfasserin aut In International Journal of Nanomedicine Dove Medical Press, 2018 (2024), Seite 2469-2485 (DE-627)537879560 (DE-600)2377464-2 11782013 nnns year:2024 pages:2469-2485 https://doaj.org/article/faaa18ebaa04464d9c8255ccf97f0ffd kostenfrei https://www.dovepress.com/highly-ordered-nanotube-like-microstructure-on-titanium-dental-implant-peer-reviewed-fulltext-article-IJN kostenfrei https://doaj.org/toc/1178-2013 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2024 2469-2485 |
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(DE-627)DOAJ092301177 (DE-599)DOAJfaaa18ebaa04464d9c8255ccf97f0ffd DE-627 ger DE-627 rakwb eng R5-920 Deng Z verfasserin aut Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing nanotubes gingival fibroblast cell behaviors cell adhesion gingival sealing Medicine (General) Yu L verfasserin aut Kuang Y verfasserin aut Zhou Z verfasserin aut Li X verfasserin aut In International Journal of Nanomedicine Dove Medical Press, 2018 (2024), Seite 2469-2485 (DE-627)537879560 (DE-600)2377464-2 11782013 nnns year:2024 pages:2469-2485 https://doaj.org/article/faaa18ebaa04464d9c8255ccf97f0ffd kostenfrei https://www.dovepress.com/highly-ordered-nanotube-like-microstructure-on-titanium-dental-implant-peer-reviewed-fulltext-article-IJN kostenfrei https://doaj.org/toc/1178-2013 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2024 2469-2485 |
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(DE-627)DOAJ092301177 (DE-599)DOAJfaaa18ebaa04464d9c8255ccf97f0ffd DE-627 ger DE-627 rakwb eng R5-920 Deng Z verfasserin aut Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing nanotubes gingival fibroblast cell behaviors cell adhesion gingival sealing Medicine (General) Yu L verfasserin aut Kuang Y verfasserin aut Zhou Z verfasserin aut Li X verfasserin aut In International Journal of Nanomedicine Dove Medical Press, 2018 (2024), Seite 2469-2485 (DE-627)537879560 (DE-600)2377464-2 11782013 nnns year:2024 pages:2469-2485 https://doaj.org/article/faaa18ebaa04464d9c8255ccf97f0ffd kostenfrei https://www.dovepress.com/highly-ordered-nanotube-like-microstructure-on-titanium-dental-implant-peer-reviewed-fulltext-article-IJN kostenfrei https://doaj.org/toc/1178-2013 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2024 2469-2485 |
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(DE-627)DOAJ092301177 (DE-599)DOAJfaaa18ebaa04464d9c8255ccf97f0ffd DE-627 ger DE-627 rakwb eng R5-920 Deng Z verfasserin aut Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing nanotubes gingival fibroblast cell behaviors cell adhesion gingival sealing Medicine (General) Yu L verfasserin aut Kuang Y verfasserin aut Zhou Z verfasserin aut Li X verfasserin aut In International Journal of Nanomedicine Dove Medical Press, 2018 (2024), Seite 2469-2485 (DE-627)537879560 (DE-600)2377464-2 11782013 nnns year:2024 pages:2469-2485 https://doaj.org/article/faaa18ebaa04464d9c8255ccf97f0ffd kostenfrei https://www.dovepress.com/highly-ordered-nanotube-like-microstructure-on-titanium-dental-implant-peer-reviewed-fulltext-article-IJN kostenfrei https://doaj.org/toc/1178-2013 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2024 2469-2485 |
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Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts |
| abstract |
Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing |
| abstractGer |
Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing |
| abstract_unstemmed |
Zhaoming Deng,1,2 Lerong Yu,1,2 Yishen Kuang,1,2 Ziyao Zhou,1,2 Xiangwei Li1 1Department of Stomatology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of China; 2Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai City, 519000, People’s Republic of ChinaCorrespondence: Xiangwei Li, The Fifth Affiliated Hospital of Sun Yat-Sen University, East Meihua Road No. 52, Xiangzhou District, Zhuhai City, Guangdong Province, People’s Republic of China, Email lixiangwei126.comBackground: Titanium (Ti) surface with nanotubes array via anodization has been used in dental implants to enhance bone regeneration but little research was carried out to evaluate whether the presence of highly ordered or disorderly distributed nanotubes array on titanium surface would have an effect on cell behaviors of gingival fibroblasts.Methods: The present study fabricated nanotubes arrays with varied topography under different constant voltage of electrochemical anodization in fluorine-containing electrolyte. Human gingival fibroblasts (HGFs) from extracted third molar were harvested and co-cultured with titanium disks with different nanotubes topography. Then cell behaviors of gingival fibroblasts including cell proliferation, adhesive morphology and cell migration were estimated to investigate the influence of titanium nanotubes on cell biology. Besides, gene and protein expression of adhesion molecule (integrin β 1/β 4/α 6, fibronectin, intracellular adhesion molecule-1 and collagen type I) were detected to evaluate the influence of different surfaces on cell adhesion.Results: Highly ordered arrays of nanotubes with pore diameter of 60 nm and 100 nm were fabricated under 30 and 40 V of anodization (TNT-30 and TNT-40) while disorderedly distributed nanotube arrays formed on the titanium surface under 50 V of anodization (TNT-50). Our results demonstrated that compared with raw titanium surface and disorderly nanotubes, surface with orderly nanotubes array increased cell area and aspect ratio, as well as cell migration ability in the early phase of cell adhesion (p< 0.05). Besides, compared with raw titanium surface, gene and protein expression of adhesion molecules were upregulated in nanotubes groups to different extents, no matter whether in an orderly or disorderly array.Conclusion: Within the limitations of our study, we conclude that compared with raw titanium surface, the presence of nanotubes array on titanium surface could enhance cells adhesion and cell migration in the early phase. And compared with disorderly distributed nanotubes, highly ordered nanotubes array might provide a much more favorable surface for gingival fibroblasts to achieve a tight adhesion on the materials. Keywords: nanotubes, gingival fibroblast, cell behaviors, cell adhesion, gingival sealing |
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| title_short |
Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts |
| url |
https://doaj.org/article/faaa18ebaa04464d9c8255ccf97f0ffd https://www.dovepress.com/highly-ordered-nanotube-like-microstructure-on-titanium-dental-implant-peer-reviewed-fulltext-article-IJN https://doaj.org/toc/1178-2013 |
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