Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines
Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer act...
Ausführliche Beschreibung
Autor*in: |
Liza Meutia Sari [verfasserIn] Dina Keumala Sari [verfasserIn] Arleni Bustami [verfasserIn] Achmad Danny Gazali [verfasserIn] Elza Ibrahim Auerkari [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch ; Spanisch |
Erschienen: |
2024 |
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Übergeordnetes Werk: |
In: Journal of Pharmacy & Pharmacognosy Research - GarVal Editorial Ltda., 2015, 12(2024), 4, Seite 659-672 |
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Übergeordnetes Werk: |
volume:12 ; year:2024 ; number:4 ; pages:659-672 |
Links: |
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DOI / URN: |
10.56499/jppres23.1798_12.4.659 |
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Katalog-ID: |
DOAJ092489311 |
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520 | |a Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer activity has not been widely studied. Aims: To evaluate the ability of P. edulis fruit seed extract to induce cytotoxicity and apoptosis in HSC-2, HSC-3, MCF-7, and HaCaT cell lines. Methods: P. edulis fruit seed extract was extracted by using 96% ethanol. The cytotoxicity activity was measured by using an MTT assay for 48 hours. Apoptosis was examined using a TUNEL assay, and the morphology of the apoptosis bodies was analyzed using electron microscopy. Results: The IC50 values of P. edulis fruit seed extract were 572.79 µg/mL and 439.54 µg/mL for HSC-3 and MCF-7, respectively. The seed did not show cytotoxic activity on the HSC-2 cell line, but it induced proliferation of the HaCaT cell line in the maximum dose. The P. edulis fruit seed extract induced apoptosis activity in HSC-3, MCF-7, and HaCaT cell lines. Most DNA fragments were found in the cytosol of HSC-3 cells and the nucleus of MCF-7 cells. Conclusions: The P. edulis fruit seed extract has cytotoxicity and apoptosis activities in oral cancer (HSC-3 cells) and breast cancer (MCF-7 cells), as well as being safe in normal human keratinocytes (HaCaT cell line). The P. edulis fruit seed extract can be considered an adjuvant agent in cancer treatment. | ||
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10.56499/jppres23.1798_12.4.659 doi (DE-627)DOAJ092489311 (DE-599)DOAJ672a86573d49402a879c926c7ecf8a03 DE-627 ger DE-627 rakwb eng spa RM1-950 RS1-441 Liza Meutia Sari verfasserin aut Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer activity has not been widely studied. Aims: To evaluate the ability of P. edulis fruit seed extract to induce cytotoxicity and apoptosis in HSC-2, HSC-3, MCF-7, and HaCaT cell lines. Methods: P. edulis fruit seed extract was extracted by using 96% ethanol. The cytotoxicity activity was measured by using an MTT assay for 48 hours. Apoptosis was examined using a TUNEL assay, and the morphology of the apoptosis bodies was analyzed using electron microscopy. Results: The IC50 values of P. edulis fruit seed extract were 572.79 µg/mL and 439.54 µg/mL for HSC-3 and MCF-7, respectively. The seed did not show cytotoxic activity on the HSC-2 cell line, but it induced proliferation of the HaCaT cell line in the maximum dose. The P. edulis fruit seed extract induced apoptosis activity in HSC-3, MCF-7, and HaCaT cell lines. Most DNA fragments were found in the cytosol of HSC-3 cells and the nucleus of MCF-7 cells. Conclusions: The P. edulis fruit seed extract has cytotoxicity and apoptosis activities in oral cancer (HSC-3 cells) and breast cancer (MCF-7 cells), as well as being safe in normal human keratinocytes (HaCaT cell line). The P. edulis fruit seed extract can be considered an adjuvant agent in cancer treatment. apoptosis breast cancer cytotoxicity oral cancer passion fruit seed Therapeutics. Pharmacology Pharmacy and materia medica Dina Keumala Sari verfasserin aut Arleni Bustami verfasserin aut Achmad Danny Gazali verfasserin aut Elza Ibrahim Auerkari verfasserin aut In Journal of Pharmacy & Pharmacognosy Research GarVal Editorial Ltda., 2015 12(2024), 4, Seite 659-672 (DE-627)830140905 (DE-600)2827786-7 07194250 nnns volume:12 year:2024 number:4 pages:659-672 https://doi.org/10.56499/jppres23.1798_12.4.659 kostenfrei https://doaj.org/article/672a86573d49402a879c926c7ecf8a03 kostenfrei https://jppres.com/jppres/pdf/vol12/jppres23.1798_12.4.659.pdf kostenfrei https://doaj.org/toc/0719-4250 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 4 659-672 |
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10.56499/jppres23.1798_12.4.659 doi (DE-627)DOAJ092489311 (DE-599)DOAJ672a86573d49402a879c926c7ecf8a03 DE-627 ger DE-627 rakwb eng spa RM1-950 RS1-441 Liza Meutia Sari verfasserin aut Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer activity has not been widely studied. Aims: To evaluate the ability of P. edulis fruit seed extract to induce cytotoxicity and apoptosis in HSC-2, HSC-3, MCF-7, and HaCaT cell lines. Methods: P. edulis fruit seed extract was extracted by using 96% ethanol. The cytotoxicity activity was measured by using an MTT assay for 48 hours. Apoptosis was examined using a TUNEL assay, and the morphology of the apoptosis bodies was analyzed using electron microscopy. Results: The IC50 values of P. edulis fruit seed extract were 572.79 µg/mL and 439.54 µg/mL for HSC-3 and MCF-7, respectively. The seed did not show cytotoxic activity on the HSC-2 cell line, but it induced proliferation of the HaCaT cell line in the maximum dose. The P. edulis fruit seed extract induced apoptosis activity in HSC-3, MCF-7, and HaCaT cell lines. Most DNA fragments were found in the cytosol of HSC-3 cells and the nucleus of MCF-7 cells. Conclusions: The P. edulis fruit seed extract has cytotoxicity and apoptosis activities in oral cancer (HSC-3 cells) and breast cancer (MCF-7 cells), as well as being safe in normal human keratinocytes (HaCaT cell line). The P. edulis fruit seed extract can be considered an adjuvant agent in cancer treatment. apoptosis breast cancer cytotoxicity oral cancer passion fruit seed Therapeutics. Pharmacology Pharmacy and materia medica Dina Keumala Sari verfasserin aut Arleni Bustami verfasserin aut Achmad Danny Gazali verfasserin aut Elza Ibrahim Auerkari verfasserin aut In Journal of Pharmacy & Pharmacognosy Research GarVal Editorial Ltda., 2015 12(2024), 4, Seite 659-672 (DE-627)830140905 (DE-600)2827786-7 07194250 nnns volume:12 year:2024 number:4 pages:659-672 https://doi.org/10.56499/jppres23.1798_12.4.659 kostenfrei https://doaj.org/article/672a86573d49402a879c926c7ecf8a03 kostenfrei https://jppres.com/jppres/pdf/vol12/jppres23.1798_12.4.659.pdf kostenfrei https://doaj.org/toc/0719-4250 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 4 659-672 |
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10.56499/jppres23.1798_12.4.659 doi (DE-627)DOAJ092489311 (DE-599)DOAJ672a86573d49402a879c926c7ecf8a03 DE-627 ger DE-627 rakwb eng spa RM1-950 RS1-441 Liza Meutia Sari verfasserin aut Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer activity has not been widely studied. Aims: To evaluate the ability of P. edulis fruit seed extract to induce cytotoxicity and apoptosis in HSC-2, HSC-3, MCF-7, and HaCaT cell lines. Methods: P. edulis fruit seed extract was extracted by using 96% ethanol. The cytotoxicity activity was measured by using an MTT assay for 48 hours. Apoptosis was examined using a TUNEL assay, and the morphology of the apoptosis bodies was analyzed using electron microscopy. Results: The IC50 values of P. edulis fruit seed extract were 572.79 µg/mL and 439.54 µg/mL for HSC-3 and MCF-7, respectively. The seed did not show cytotoxic activity on the HSC-2 cell line, but it induced proliferation of the HaCaT cell line in the maximum dose. The P. edulis fruit seed extract induced apoptosis activity in HSC-3, MCF-7, and HaCaT cell lines. Most DNA fragments were found in the cytosol of HSC-3 cells and the nucleus of MCF-7 cells. Conclusions: The P. edulis fruit seed extract has cytotoxicity and apoptosis activities in oral cancer (HSC-3 cells) and breast cancer (MCF-7 cells), as well as being safe in normal human keratinocytes (HaCaT cell line). The P. edulis fruit seed extract can be considered an adjuvant agent in cancer treatment. apoptosis breast cancer cytotoxicity oral cancer passion fruit seed Therapeutics. Pharmacology Pharmacy and materia medica Dina Keumala Sari verfasserin aut Arleni Bustami verfasserin aut Achmad Danny Gazali verfasserin aut Elza Ibrahim Auerkari verfasserin aut In Journal of Pharmacy & Pharmacognosy Research GarVal Editorial Ltda., 2015 12(2024), 4, Seite 659-672 (DE-627)830140905 (DE-600)2827786-7 07194250 nnns volume:12 year:2024 number:4 pages:659-672 https://doi.org/10.56499/jppres23.1798_12.4.659 kostenfrei https://doaj.org/article/672a86573d49402a879c926c7ecf8a03 kostenfrei https://jppres.com/jppres/pdf/vol12/jppres23.1798_12.4.659.pdf kostenfrei https://doaj.org/toc/0719-4250 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 4 659-672 |
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10.56499/jppres23.1798_12.4.659 doi (DE-627)DOAJ092489311 (DE-599)DOAJ672a86573d49402a879c926c7ecf8a03 DE-627 ger DE-627 rakwb eng spa RM1-950 RS1-441 Liza Meutia Sari verfasserin aut Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer activity has not been widely studied. Aims: To evaluate the ability of P. edulis fruit seed extract to induce cytotoxicity and apoptosis in HSC-2, HSC-3, MCF-7, and HaCaT cell lines. Methods: P. edulis fruit seed extract was extracted by using 96% ethanol. The cytotoxicity activity was measured by using an MTT assay for 48 hours. Apoptosis was examined using a TUNEL assay, and the morphology of the apoptosis bodies was analyzed using electron microscopy. Results: The IC50 values of P. edulis fruit seed extract were 572.79 µg/mL and 439.54 µg/mL for HSC-3 and MCF-7, respectively. The seed did not show cytotoxic activity on the HSC-2 cell line, but it induced proliferation of the HaCaT cell line in the maximum dose. The P. edulis fruit seed extract induced apoptosis activity in HSC-3, MCF-7, and HaCaT cell lines. Most DNA fragments were found in the cytosol of HSC-3 cells and the nucleus of MCF-7 cells. Conclusions: The P. edulis fruit seed extract has cytotoxicity and apoptosis activities in oral cancer (HSC-3 cells) and breast cancer (MCF-7 cells), as well as being safe in normal human keratinocytes (HaCaT cell line). The P. edulis fruit seed extract can be considered an adjuvant agent in cancer treatment. apoptosis breast cancer cytotoxicity oral cancer passion fruit seed Therapeutics. Pharmacology Pharmacy and materia medica Dina Keumala Sari verfasserin aut Arleni Bustami verfasserin aut Achmad Danny Gazali verfasserin aut Elza Ibrahim Auerkari verfasserin aut In Journal of Pharmacy & Pharmacognosy Research GarVal Editorial Ltda., 2015 12(2024), 4, Seite 659-672 (DE-627)830140905 (DE-600)2827786-7 07194250 nnns volume:12 year:2024 number:4 pages:659-672 https://doi.org/10.56499/jppres23.1798_12.4.659 kostenfrei https://doaj.org/article/672a86573d49402a879c926c7ecf8a03 kostenfrei https://jppres.com/jppres/pdf/vol12/jppres23.1798_12.4.659.pdf kostenfrei https://doaj.org/toc/0719-4250 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 4 659-672 |
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Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines |
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Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer activity has not been widely studied. Aims: To evaluate the ability of P. edulis fruit seed extract to induce cytotoxicity and apoptosis in HSC-2, HSC-3, MCF-7, and HaCaT cell lines. Methods: P. edulis fruit seed extract was extracted by using 96% ethanol. The cytotoxicity activity was measured by using an MTT assay for 48 hours. Apoptosis was examined using a TUNEL assay, and the morphology of the apoptosis bodies was analyzed using electron microscopy. Results: The IC50 values of P. edulis fruit seed extract were 572.79 µg/mL and 439.54 µg/mL for HSC-3 and MCF-7, respectively. The seed did not show cytotoxic activity on the HSC-2 cell line, but it induced proliferation of the HaCaT cell line in the maximum dose. The P. edulis fruit seed extract induced apoptosis activity in HSC-3, MCF-7, and HaCaT cell lines. Most DNA fragments were found in the cytosol of HSC-3 cells and the nucleus of MCF-7 cells. Conclusions: The P. edulis fruit seed extract has cytotoxicity and apoptosis activities in oral cancer (HSC-3 cells) and breast cancer (MCF-7 cells), as well as being safe in normal human keratinocytes (HaCaT cell line). The P. edulis fruit seed extract can be considered an adjuvant agent in cancer treatment. |
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Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer activity has not been widely studied. Aims: To evaluate the ability of P. edulis fruit seed extract to induce cytotoxicity and apoptosis in HSC-2, HSC-3, MCF-7, and HaCaT cell lines. Methods: P. edulis fruit seed extract was extracted by using 96% ethanol. The cytotoxicity activity was measured by using an MTT assay for 48 hours. Apoptosis was examined using a TUNEL assay, and the morphology of the apoptosis bodies was analyzed using electron microscopy. Results: The IC50 values of P. edulis fruit seed extract were 572.79 µg/mL and 439.54 µg/mL for HSC-3 and MCF-7, respectively. The seed did not show cytotoxic activity on the HSC-2 cell line, but it induced proliferation of the HaCaT cell line in the maximum dose. The P. edulis fruit seed extract induced apoptosis activity in HSC-3, MCF-7, and HaCaT cell lines. Most DNA fragments were found in the cytosol of HSC-3 cells and the nucleus of MCF-7 cells. Conclusions: The P. edulis fruit seed extract has cytotoxicity and apoptosis activities in oral cancer (HSC-3 cells) and breast cancer (MCF-7 cells), as well as being safe in normal human keratinocytes (HaCaT cell line). The P. edulis fruit seed extract can be considered an adjuvant agent in cancer treatment. |
abstract_unstemmed |
Context: Passion fruit (Passiflora edulis Sims) is a well-known plant in the tropical islands, especially in North Sumatra, Indonesia, because of its sweet and refreshing taste. Although the fruit is often used as a syrup and sweetener, the efficacy of P. edulis fruit seed extract for anticancer activity has not been widely studied. Aims: To evaluate the ability of P. edulis fruit seed extract to induce cytotoxicity and apoptosis in HSC-2, HSC-3, MCF-7, and HaCaT cell lines. Methods: P. edulis fruit seed extract was extracted by using 96% ethanol. The cytotoxicity activity was measured by using an MTT assay for 48 hours. Apoptosis was examined using a TUNEL assay, and the morphology of the apoptosis bodies was analyzed using electron microscopy. Results: The IC50 values of P. edulis fruit seed extract were 572.79 µg/mL and 439.54 µg/mL for HSC-3 and MCF-7, respectively. The seed did not show cytotoxic activity on the HSC-2 cell line, but it induced proliferation of the HaCaT cell line in the maximum dose. The P. edulis fruit seed extract induced apoptosis activity in HSC-3, MCF-7, and HaCaT cell lines. Most DNA fragments were found in the cytosol of HSC-3 cells and the nucleus of MCF-7 cells. Conclusions: The P. edulis fruit seed extract has cytotoxicity and apoptosis activities in oral cancer (HSC-3 cells) and breast cancer (MCF-7 cells), as well as being safe in normal human keratinocytes (HaCaT cell line). The P. edulis fruit seed extract can be considered an adjuvant agent in cancer treatment. |
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Cytotoxicity and apoptosis activities of passion fruit (Passiflora edulis Sims) seed extract on HSC-2, HSC-3, MCF-7, and HaCaT cell lines |
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https://doi.org/10.56499/jppres23.1798_12.4.659 https://doaj.org/article/672a86573d49402a879c926c7ecf8a03 https://jppres.com/jppres/pdf/vol12/jppres23.1798_12.4.659.pdf https://doaj.org/toc/0719-4250 |
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Dina Keumala Sari Arleni Bustami Achmad Danny Gazali Elza Ibrahim Auerkari |
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