Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil

ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other dome...
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Autor*in:	Maiara C. Lima [verfasserIn] Roberta M. Basso [verfasserIn] Fabrício M. Cerri [verfasserIn] Heloísa C. Lima [verfasserIn] Sheila C. Rahal [verfasserIn] Isabela P. Zanon [verfasserIn] Gabriela M. Carvalho [verfasserIn] Rodrigo Otávio S. Silva [verfasserIn] Luis G. Arroyo [verfasserIn] José P. Oliveira-Filho [verfasserIn] Alexandre S. Borges [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Portugiesisch

Erschienen:	2024

Schlagwörter:	Didelphis spp. qPCR tcdB tcdA wild animals Clostridioides difficile

Übergeordnetes Werk:	In: Pesquisa Veterinária Brasileira - Colégio Brasileiro de Patologia Animal (CBPA), 2004, 44(2024)
Übergeordnetes Werk:	volume:44 ; year:2024

Links:	Link aufrufen Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/1678-5150-pvb-7385

Katalog-ID:	DOAJ092828671

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520			\|a ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans.
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allfields	10.1590/1678-5150-pvb-7385 doi (DE-627)DOAJ092828671 (DE-599)DOAJ7d26e8bf9719493bab87b0ae81efe22c DE-627 ger DE-627 rakwb eng por SF600-1100 Maiara C. Lima verfasserin aut Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans. Didelphis spp. qPCR tcdB tcdA wild animals Clostridioides difficile Veterinary medicine Roberta M. Basso verfasserin aut Fabrício M. Cerri verfasserin aut Heloísa C. Lima verfasserin aut Sheila C. Rahal verfasserin aut Isabela P. Zanon verfasserin aut Gabriela M. Carvalho verfasserin aut Rodrigo Otávio S. Silva verfasserin aut Luis G. Arroyo verfasserin aut José P. Oliveira-Filho verfasserin aut Alexandre S. Borges verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 44(2024) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:44 year:2024 https://doi.org/10.1590/1678-5150-pvb-7385 kostenfrei https://doaj.org/article/7d26e8bf9719493bab87b0ae81efe22c kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100903&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v44/1678-5150-pvb-44-e07385.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2024
spelling	10.1590/1678-5150-pvb-7385 doi (DE-627)DOAJ092828671 (DE-599)DOAJ7d26e8bf9719493bab87b0ae81efe22c DE-627 ger DE-627 rakwb eng por SF600-1100 Maiara C. Lima verfasserin aut Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans. Didelphis spp. qPCR tcdB tcdA wild animals Clostridioides difficile Veterinary medicine Roberta M. Basso verfasserin aut Fabrício M. Cerri verfasserin aut Heloísa C. Lima verfasserin aut Sheila C. Rahal verfasserin aut Isabela P. Zanon verfasserin aut Gabriela M. Carvalho verfasserin aut Rodrigo Otávio S. Silva verfasserin aut Luis G. Arroyo verfasserin aut José P. Oliveira-Filho verfasserin aut Alexandre S. Borges verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 44(2024) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:44 year:2024 https://doi.org/10.1590/1678-5150-pvb-7385 kostenfrei https://doaj.org/article/7d26e8bf9719493bab87b0ae81efe22c kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100903&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v44/1678-5150-pvb-44-e07385.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2024
allfields_unstemmed	10.1590/1678-5150-pvb-7385 doi (DE-627)DOAJ092828671 (DE-599)DOAJ7d26e8bf9719493bab87b0ae81efe22c DE-627 ger DE-627 rakwb eng por SF600-1100 Maiara C. Lima verfasserin aut Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans. Didelphis spp. qPCR tcdB tcdA wild animals Clostridioides difficile Veterinary medicine Roberta M. Basso verfasserin aut Fabrício M. Cerri verfasserin aut Heloísa C. Lima verfasserin aut Sheila C. Rahal verfasserin aut Isabela P. Zanon verfasserin aut Gabriela M. Carvalho verfasserin aut Rodrigo Otávio S. Silva verfasserin aut Luis G. Arroyo verfasserin aut José P. Oliveira-Filho verfasserin aut Alexandre S. Borges verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 44(2024) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:44 year:2024 https://doi.org/10.1590/1678-5150-pvb-7385 kostenfrei https://doaj.org/article/7d26e8bf9719493bab87b0ae81efe22c kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100903&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v44/1678-5150-pvb-44-e07385.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2024
allfieldsGer	10.1590/1678-5150-pvb-7385 doi (DE-627)DOAJ092828671 (DE-599)DOAJ7d26e8bf9719493bab87b0ae81efe22c DE-627 ger DE-627 rakwb eng por SF600-1100 Maiara C. Lima verfasserin aut Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans. Didelphis spp. qPCR tcdB tcdA wild animals Clostridioides difficile Veterinary medicine Roberta M. Basso verfasserin aut Fabrício M. Cerri verfasserin aut Heloísa C. Lima verfasserin aut Sheila C. Rahal verfasserin aut Isabela P. Zanon verfasserin aut Gabriela M. Carvalho verfasserin aut Rodrigo Otávio S. Silva verfasserin aut Luis G. Arroyo verfasserin aut José P. Oliveira-Filho verfasserin aut Alexandre S. Borges verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 44(2024) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:44 year:2024 https://doi.org/10.1590/1678-5150-pvb-7385 kostenfrei https://doaj.org/article/7d26e8bf9719493bab87b0ae81efe22c kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100903&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v44/1678-5150-pvb-44-e07385.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2024
allfieldsSound	10.1590/1678-5150-pvb-7385 doi (DE-627)DOAJ092828671 (DE-599)DOAJ7d26e8bf9719493bab87b0ae81efe22c DE-627 ger DE-627 rakwb eng por SF600-1100 Maiara C. Lima verfasserin aut Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans. Didelphis spp. qPCR tcdB tcdA wild animals Clostridioides difficile Veterinary medicine Roberta M. Basso verfasserin aut Fabrício M. Cerri verfasserin aut Heloísa C. Lima verfasserin aut Sheila C. Rahal verfasserin aut Isabela P. Zanon verfasserin aut Gabriela M. Carvalho verfasserin aut Rodrigo Otávio S. Silva verfasserin aut Luis G. Arroyo verfasserin aut José P. Oliveira-Filho verfasserin aut Alexandre S. Borges verfasserin aut In Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA), 2004 44(2024) (DE-627)324613520 (DE-600)2028762-8 16785150 nnns volume:44 year:2024 https://doi.org/10.1590/1678-5150-pvb-7385 kostenfrei https://doaj.org/article/7d26e8bf9719493bab87b0ae81efe22c kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100903&lng=en&tlng=en kostenfrei http://www.scielo.br/pdf/pvb/v44/1678-5150-pvb-44-e07385.pdf kostenfrei https://doaj.org/toc/1678-5150 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 44 2024
language	English Portuguese
source	In Pesquisa Veterinária Brasileira 44(2024) volume:44 year:2024
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topic_facet	Didelphis spp. qPCR tcdB tcdA wild animals Clostridioides difficile Veterinary medicine
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container_title	Pesquisa Veterinária Brasileira
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topic_title	SF600-1100 Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil Didelphis spp qPCR tcdB tcdA wild animals Clostridioides difficile
topic	misc SF600-1100 misc Didelphis spp. misc qPCR misc tcdB misc tcdA misc wild animals misc Clostridioides difficile misc Veterinary medicine
topic_unstemmed	misc SF600-1100 misc Didelphis spp. misc qPCR misc tcdB misc tcdA misc wild animals misc Clostridioides difficile misc Veterinary medicine
topic_browse	misc SF600-1100 misc Didelphis spp. misc qPCR misc tcdB misc tcdA misc wild animals misc Clostridioides difficile misc Veterinary medicine
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title	Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil
ctrlnum	(DE-627)DOAJ092828671 (DE-599)DOAJ7d26e8bf9719493bab87b0ae81efe22c
title_full	Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil
author_sort	Maiara C. Lima
journal	Pesquisa Veterinária Brasileira
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author_browse	Maiara C. Lima Roberta M. Basso Fabrício M. Cerri Heloísa C. Lima Sheila C. Rahal Isabela P. Zanon Gabriela M. Carvalho Rodrigo Otávio S. Silva Luis G. Arroyo José P. Oliveira-Filho Alexandre S. Borges
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doi_str_mv	10.1590/1678-5150-pvb-7385
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title_sort	molecular epidemiology of clostridioides difficile obtained from fecal samples of wild animals in brazil
callnumber	SF600-1100
title_auth	Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil
abstract	ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans.
abstractGer	ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans.
abstract_unstemmed	ABSTRACT: Clostridioides difficile is a strictly anaerobic, spore-forming Gram-positive bacterium associated with diarrhea, known as C. difficile infection (CDI). In domestic animals, C. difficile is considered an important pathogen mostly in pigs and horses, but there are also reports in other domestic species. In wild animals, the epidemiology of C. difficile is largely unknown, and the role of the bacterium as a cause of diarrhea is unclear. The aim of this study was to determine the prevalence of C. difficile in the feces of wild animals referred to the Center of Medicine and Research in Wild Animals (CEMPAS). Fecal samples obtained from 100 animals of 34 different species were subjected to qPCR for the detection of the C. difficile 16S rRNA gene and two major toxin genes (tcdA and tcdB) and to anaerobic bacterial isolation. A total of 63 animals (63%) were positive for C. difficile by qPCR, and 16 isolates were recovered. The opossum (Didelphis spp.) had the highest number of positive animals in both tests (from 21 samples, 19 were qPCR positive, and four isolates were recovered). Three toxigenic strains (RT 002, 004, and 014), all previously described as infecting humans and animals, were isolated in the following species: bearded dragon (Pogona vitticeps), pampas fox (Lycalopex vetulus), and marmoset (Callithrix sp.). The presence of C. difficile in the feces of wild animals highlights the importance of wildlife as potential carriers of infection for production animals or humans.
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title_short	Molecular epidemiology of Clostridioides difficile obtained from fecal samples of wild animals in Brazil
url	https://doi.org/10.1590/1678-5150-pvb-7385 https://doaj.org/article/7d26e8bf9719493bab87b0ae81efe22c http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100903&lng=en&tlng=en http://www.scielo.br/pdf/pvb/v44/1678-5150-pvb-44-e07385.pdf https://doaj.org/toc/1678-5150
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author2	Roberta M. Basso Fabrício M. Cerri Heloísa C. Lima Sheila C. Rahal Isabela P. Zanon Gabriela M. Carvalho Rodrigo Otávio S. Silva Luis G. Arroyo José P. Oliveira-Filho Alexandre S. Borges
author2Str	Roberta M. Basso Fabrício M. Cerri Heloísa C. Lima Sheila C. Rahal Isabela P. Zanon Gabriela M. Carvalho Rodrigo Otávio S. Silva Luis G. Arroyo José P. Oliveira-Filho Alexandre S. Borges
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up_date	2024-07-03T13:48:33.069Z
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