Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae)

Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in as...
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Autor*in:	Godfrey P. Miles [verfasserIn] Xiaofen F. Liu [verfasserIn] Esmaeil Amiri [verfasserIn] Michael J. Grodowitz [verfasserIn] Margaret L. Allen [verfasserIn] Jian Chen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	deformed wing syndrome bee deformed wing virus pollinators viral replication

Übergeordnetes Werk:	In: Insects - MDPI AG, 2011, 14(2023), 788, p 788
Übergeordnetes Werk:	volume:14 ; year:2023 ; number:788, p 788

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/insects14100788

Katalog-ID:	DOAJ093128592

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520			\|a Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<.
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allfields	10.3390/insects14100788 doi (DE-627)DOAJ093128592 (DE-599)DOAJ209ec8c23b6843139844d0d845291767 DE-627 ger DE-627 rakwb eng Godfrey P. Miles verfasserin aut Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<. <i<Solenopsis invicta</i< deformed wing syndrome bee deformed wing virus pollinators viral replication Science Q Xiaofen F. Liu verfasserin aut Esmaeil Amiri verfasserin aut Michael J. Grodowitz verfasserin aut Margaret L. Allen verfasserin aut Jian Chen verfasserin aut In Insects MDPI AG, 2011 14(2023), 788, p 788 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:788, p 788 https://doi.org/10.3390/insects14100788 kostenfrei https://doaj.org/article/209ec8c23b6843139844d0d845291767 kostenfrei https://www.mdpi.com/2075-4450/14/10/788 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 788, p 788
spelling	10.3390/insects14100788 doi (DE-627)DOAJ093128592 (DE-599)DOAJ209ec8c23b6843139844d0d845291767 DE-627 ger DE-627 rakwb eng Godfrey P. Miles verfasserin aut Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<. <i<Solenopsis invicta</i< deformed wing syndrome bee deformed wing virus pollinators viral replication Science Q Xiaofen F. Liu verfasserin aut Esmaeil Amiri verfasserin aut Michael J. Grodowitz verfasserin aut Margaret L. Allen verfasserin aut Jian Chen verfasserin aut In Insects MDPI AG, 2011 14(2023), 788, p 788 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:788, p 788 https://doi.org/10.3390/insects14100788 kostenfrei https://doaj.org/article/209ec8c23b6843139844d0d845291767 kostenfrei https://www.mdpi.com/2075-4450/14/10/788 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 788, p 788
allfields_unstemmed	10.3390/insects14100788 doi (DE-627)DOAJ093128592 (DE-599)DOAJ209ec8c23b6843139844d0d845291767 DE-627 ger DE-627 rakwb eng Godfrey P. Miles verfasserin aut Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<. <i<Solenopsis invicta</i< deformed wing syndrome bee deformed wing virus pollinators viral replication Science Q Xiaofen F. Liu verfasserin aut Esmaeil Amiri verfasserin aut Michael J. Grodowitz verfasserin aut Margaret L. Allen verfasserin aut Jian Chen verfasserin aut In Insects MDPI AG, 2011 14(2023), 788, p 788 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:788, p 788 https://doi.org/10.3390/insects14100788 kostenfrei https://doaj.org/article/209ec8c23b6843139844d0d845291767 kostenfrei https://www.mdpi.com/2075-4450/14/10/788 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 788, p 788
allfieldsGer	10.3390/insects14100788 doi (DE-627)DOAJ093128592 (DE-599)DOAJ209ec8c23b6843139844d0d845291767 DE-627 ger DE-627 rakwb eng Godfrey P. Miles verfasserin aut Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<. <i<Solenopsis invicta</i< deformed wing syndrome bee deformed wing virus pollinators viral replication Science Q Xiaofen F. Liu verfasserin aut Esmaeil Amiri verfasserin aut Michael J. Grodowitz verfasserin aut Margaret L. Allen verfasserin aut Jian Chen verfasserin aut In Insects MDPI AG, 2011 14(2023), 788, p 788 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:788, p 788 https://doi.org/10.3390/insects14100788 kostenfrei https://doaj.org/article/209ec8c23b6843139844d0d845291767 kostenfrei https://www.mdpi.com/2075-4450/14/10/788 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 788, p 788
allfieldsSound	10.3390/insects14100788 doi (DE-627)DOAJ093128592 (DE-599)DOAJ209ec8c23b6843139844d0d845291767 DE-627 ger DE-627 rakwb eng Godfrey P. Miles verfasserin aut Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<. <i<Solenopsis invicta</i< deformed wing syndrome bee deformed wing virus pollinators viral replication Science Q Xiaofen F. Liu verfasserin aut Esmaeil Amiri verfasserin aut Michael J. Grodowitz verfasserin aut Margaret L. Allen verfasserin aut Jian Chen verfasserin aut In Insects MDPI AG, 2011 14(2023), 788, p 788 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:788, p 788 https://doi.org/10.3390/insects14100788 kostenfrei https://doaj.org/article/209ec8c23b6843139844d0d845291767 kostenfrei https://www.mdpi.com/2075-4450/14/10/788 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 788, p 788
language	English
source	In Insects 14(2023), 788, p 788 volume:14 year:2023 number:788, p 788
sourceStr	In Insects 14(2023), 788, p 788 volume:14 year:2023 number:788, p 788
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Solenopsis invicta</i< deformed wing syndrome bee deformed wing virus pollinators viral replication Science Q
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container_title	Insects
authorswithroles_txt_mv	Godfrey P. Miles @@aut@@ Xiaofen F. Liu @@aut@@ Esmaeil Amiri @@aut@@ Michael J. Grodowitz @@aut@@ Margaret L. Allen @@aut@@ Jian Chen @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
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author	Godfrey P. Miles
spellingShingle	Godfrey P. Miles misc <i<Solenopsis invicta</i< misc deformed wing syndrome misc bee deformed wing virus misc pollinators misc viral replication misc Science misc Q Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae)
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topic_title	Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae) <i<Solenopsis invicta</i< deformed wing syndrome bee deformed wing virus pollinators viral replication
topic	misc <i<Solenopsis invicta</i< misc deformed wing syndrome misc bee deformed wing virus misc pollinators misc viral replication misc Science misc Q
topic_unstemmed	misc <i<Solenopsis invicta</i< misc deformed wing syndrome misc bee deformed wing virus misc pollinators misc viral replication misc Science misc Q
topic_browse	misc <i<Solenopsis invicta</i< misc deformed wing syndrome misc bee deformed wing virus misc pollinators misc viral replication misc Science misc Q
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title	Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae)
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title_full	Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae)
author_sort	Godfrey P. Miles
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author_browse	Godfrey P. Miles Xiaofen F. Liu Esmaeil Amiri Michael J. Grodowitz Margaret L. Allen Jian Chen
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title_sort	co-occurrence of wing deformity and impaired mobility of alates with deformed wing virus in <i<solenopsis invicta</i< buren (hymenoptera: formicidae)
title_auth	Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae)
abstract	Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<.
abstractGer	Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<.
abstract_unstemmed	Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, <i<Varroa destructor</i<, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, <i<Solenopsis invicta</i< Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in <i<S. invicta</i< and DWV for the first time and provide molecular evidence of viral replication <i<in S. invicta</i<. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent <i<S. invicta</i< alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that <i<S. invicta</i< can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in <i<S. invicta</i<.
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container_issue	788, p 788
title_short	Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae)
url	https://doi.org/10.3390/insects14100788 https://doaj.org/article/209ec8c23b6843139844d0d845291767 https://www.mdpi.com/2075-4450/14/10/788 https://doaj.org/toc/2075-4450
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Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in <i<Solenopsis invicta</i< Buren (Hymenoptera: Formicidae)

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