Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice

This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yell...
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Autor*in:	Ping Li [verfasserIn] Nana Xu [verfasserIn] Yang Shui [verfasserIn] Jie Zhang [verfasserIn] Wuzhong Yin [verfasserIn] Min Tian [verfasserIn] Faping Guo [verfasserIn] Dasong Bai [verfasserIn] Pan Qi [verfasserIn] Qingxiong Huang [verfasserIn] Biluo Li [verfasserIn] Yuanyuan Li [verfasserIn] Yungao Hu [verfasserIn] Youlin Peng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	rice lesion-mimic mutant root ROS splicing factor 3b subunit 3 RT-qPCR

Übergeordnetes Werk:	In: Agriculture - MDPI AG, 2012, 13(2023), 1875, p 1875
Übergeordnetes Werk:	volume:13 ; year:2023 ; number:1875, p 1875

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/agriculture13101875

Katalog-ID:	DOAJ09319207X

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allfields	10.3390/agriculture13101875 doi (DE-627)DOAJ09319207X (DE-599)DOAJef48172d98e34037a68c80853b61dd82 DE-627 ger DE-627 rakwb eng S1-972 Ping Li verfasserin aut Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, <i<lm5</i< had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of <i<lm5</i<, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of <i<LOC_Os07g10390</i< (<i<OsLM5</i<) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The <i<OsLM5</i< gene was found to be a new allele of the <i<SPL5</i< gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and <i<OsLM5</i< was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant <i<lm5</i<, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The <i<OsLM5</i< gene may have a significant impact on the regulation of apoptosis in rice cells. rice lesion-mimic mutant root ROS splicing factor 3b subunit 3 RT-qPCR Agriculture (General) Nana Xu verfasserin aut Yang Shui verfasserin aut Jie Zhang verfasserin aut Wuzhong Yin verfasserin aut Min Tian verfasserin aut Faping Guo verfasserin aut Dasong Bai verfasserin aut Pan Qi verfasserin aut Qingxiong Huang verfasserin aut Biluo Li verfasserin aut Yuanyuan Li verfasserin aut Yungao Hu verfasserin aut Youlin Peng verfasserin aut In Agriculture MDPI AG, 2012 13(2023), 1875, p 1875 (DE-627)686948173 (DE-600)2651678-0 20770472 nnns volume:13 year:2023 number:1875, p 1875 https://doi.org/10.3390/agriculture13101875 kostenfrei https://doaj.org/article/ef48172d98e34037a68c80853b61dd82 kostenfrei https://www.mdpi.com/2077-0472/13/10/1875 kostenfrei https://doaj.org/toc/2077-0472 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1875, p 1875
spelling	10.3390/agriculture13101875 doi (DE-627)DOAJ09319207X (DE-599)DOAJef48172d98e34037a68c80853b61dd82 DE-627 ger DE-627 rakwb eng S1-972 Ping Li verfasserin aut Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, <i<lm5</i< had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of <i<lm5</i<, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of <i<LOC_Os07g10390</i< (<i<OsLM5</i<) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The <i<OsLM5</i< gene was found to be a new allele of the <i<SPL5</i< gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and <i<OsLM5</i< was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant <i<lm5</i<, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The <i<OsLM5</i< gene may have a significant impact on the regulation of apoptosis in rice cells. rice lesion-mimic mutant root ROS splicing factor 3b subunit 3 RT-qPCR Agriculture (General) Nana Xu verfasserin aut Yang Shui verfasserin aut Jie Zhang verfasserin aut Wuzhong Yin verfasserin aut Min Tian verfasserin aut Faping Guo verfasserin aut Dasong Bai verfasserin aut Pan Qi verfasserin aut Qingxiong Huang verfasserin aut Biluo Li verfasserin aut Yuanyuan Li verfasserin aut Yungao Hu verfasserin aut Youlin Peng verfasserin aut In Agriculture MDPI AG, 2012 13(2023), 1875, p 1875 (DE-627)686948173 (DE-600)2651678-0 20770472 nnns volume:13 year:2023 number:1875, p 1875 https://doi.org/10.3390/agriculture13101875 kostenfrei https://doaj.org/article/ef48172d98e34037a68c80853b61dd82 kostenfrei https://www.mdpi.com/2077-0472/13/10/1875 kostenfrei https://doaj.org/toc/2077-0472 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1875, p 1875
allfields_unstemmed	10.3390/agriculture13101875 doi (DE-627)DOAJ09319207X (DE-599)DOAJef48172d98e34037a68c80853b61dd82 DE-627 ger DE-627 rakwb eng S1-972 Ping Li verfasserin aut Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, <i<lm5</i< had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of <i<lm5</i<, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of <i<LOC_Os07g10390</i< (<i<OsLM5</i<) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The <i<OsLM5</i< gene was found to be a new allele of the <i<SPL5</i< gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and <i<OsLM5</i< was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant <i<lm5</i<, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The <i<OsLM5</i< gene may have a significant impact on the regulation of apoptosis in rice cells. rice lesion-mimic mutant root ROS splicing factor 3b subunit 3 RT-qPCR Agriculture (General) Nana Xu verfasserin aut Yang Shui verfasserin aut Jie Zhang verfasserin aut Wuzhong Yin verfasserin aut Min Tian verfasserin aut Faping Guo verfasserin aut Dasong Bai verfasserin aut Pan Qi verfasserin aut Qingxiong Huang verfasserin aut Biluo Li verfasserin aut Yuanyuan Li verfasserin aut Yungao Hu verfasserin aut Youlin Peng verfasserin aut In Agriculture MDPI AG, 2012 13(2023), 1875, p 1875 (DE-627)686948173 (DE-600)2651678-0 20770472 nnns volume:13 year:2023 number:1875, p 1875 https://doi.org/10.3390/agriculture13101875 kostenfrei https://doaj.org/article/ef48172d98e34037a68c80853b61dd82 kostenfrei https://www.mdpi.com/2077-0472/13/10/1875 kostenfrei https://doaj.org/toc/2077-0472 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1875, p 1875
allfieldsGer	10.3390/agriculture13101875 doi (DE-627)DOAJ09319207X (DE-599)DOAJef48172d98e34037a68c80853b61dd82 DE-627 ger DE-627 rakwb eng S1-972 Ping Li verfasserin aut Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, <i<lm5</i< had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of <i<lm5</i<, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of <i<LOC_Os07g10390</i< (<i<OsLM5</i<) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The <i<OsLM5</i< gene was found to be a new allele of the <i<SPL5</i< gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and <i<OsLM5</i< was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant <i<lm5</i<, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The <i<OsLM5</i< gene may have a significant impact on the regulation of apoptosis in rice cells. rice lesion-mimic mutant root ROS splicing factor 3b subunit 3 RT-qPCR Agriculture (General) Nana Xu verfasserin aut Yang Shui verfasserin aut Jie Zhang verfasserin aut Wuzhong Yin verfasserin aut Min Tian verfasserin aut Faping Guo verfasserin aut Dasong Bai verfasserin aut Pan Qi verfasserin aut Qingxiong Huang verfasserin aut Biluo Li verfasserin aut Yuanyuan Li verfasserin aut Yungao Hu verfasserin aut Youlin Peng verfasserin aut In Agriculture MDPI AG, 2012 13(2023), 1875, p 1875 (DE-627)686948173 (DE-600)2651678-0 20770472 nnns volume:13 year:2023 number:1875, p 1875 https://doi.org/10.3390/agriculture13101875 kostenfrei https://doaj.org/article/ef48172d98e34037a68c80853b61dd82 kostenfrei https://www.mdpi.com/2077-0472/13/10/1875 kostenfrei https://doaj.org/toc/2077-0472 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1875, p 1875
allfieldsSound	10.3390/agriculture13101875 doi (DE-627)DOAJ09319207X (DE-599)DOAJef48172d98e34037a68c80853b61dd82 DE-627 ger DE-627 rakwb eng S1-972 Ping Li verfasserin aut Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, <i<lm5</i< had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of <i<lm5</i<, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of <i<LOC_Os07g10390</i< (<i<OsLM5</i<) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The <i<OsLM5</i< gene was found to be a new allele of the <i<SPL5</i< gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and <i<OsLM5</i< was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant <i<lm5</i<, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The <i<OsLM5</i< gene may have a significant impact on the regulation of apoptosis in rice cells. rice lesion-mimic mutant root ROS splicing factor 3b subunit 3 RT-qPCR Agriculture (General) Nana Xu verfasserin aut Yang Shui verfasserin aut Jie Zhang verfasserin aut Wuzhong Yin verfasserin aut Min Tian verfasserin aut Faping Guo verfasserin aut Dasong Bai verfasserin aut Pan Qi verfasserin aut Qingxiong Huang verfasserin aut Biluo Li verfasserin aut Yuanyuan Li verfasserin aut Yungao Hu verfasserin aut Youlin Peng verfasserin aut In Agriculture MDPI AG, 2012 13(2023), 1875, p 1875 (DE-627)686948173 (DE-600)2651678-0 20770472 nnns volume:13 year:2023 number:1875, p 1875 https://doi.org/10.3390/agriculture13101875 kostenfrei https://doaj.org/article/ef48172d98e34037a68c80853b61dd82 kostenfrei https://www.mdpi.com/2077-0472/13/10/1875 kostenfrei https://doaj.org/toc/2077-0472 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 1875, p 1875
language	English
source	In Agriculture 13(2023), 1875, p 1875 volume:13 year:2023 number:1875, p 1875
sourceStr	In Agriculture 13(2023), 1875, p 1875 volume:13 year:2023 number:1875, p 1875
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	rice lesion-mimic mutant root ROS splicing factor 3b subunit 3 RT-qPCR Agriculture (General)
isfreeaccess_bool	true
container_title	Agriculture
authorswithroles_txt_mv	Ping Li @@aut@@ Nana Xu @@aut@@ Yang Shui @@aut@@ Jie Zhang @@aut@@ Wuzhong Yin @@aut@@ Min Tian @@aut@@ Faping Guo @@aut@@ Dasong Bai @@aut@@ Pan Qi @@aut@@ Qingxiong Huang @@aut@@ Biluo Li @@aut@@ Yuanyuan Li @@aut@@ Yungao Hu @@aut@@ Youlin Peng @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	686948173
id	DOAJ09319207X
language_de	englisch
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callnumber-first	S - Agriculture
author	Ping Li
spellingShingle	Ping Li misc S1-972 misc rice misc lesion-mimic mutant misc root misc ROS misc splicing factor 3b subunit 3 misc RT-qPCR misc Agriculture (General) Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice
authorStr	Ping Li
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topic_title	S1-972 Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice rice lesion-mimic mutant root ROS splicing factor 3b subunit 3 RT-qPCR
topic	misc S1-972 misc rice misc lesion-mimic mutant misc root misc ROS misc splicing factor 3b subunit 3 misc RT-qPCR misc Agriculture (General)
topic_unstemmed	misc S1-972 misc rice misc lesion-mimic mutant misc root misc ROS misc splicing factor 3b subunit 3 misc RT-qPCR misc Agriculture (General)
topic_browse	misc S1-972 misc rice misc lesion-mimic mutant misc root misc ROS misc splicing factor 3b subunit 3 misc RT-qPCR misc Agriculture (General)
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title	Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice
ctrlnum	(DE-627)DOAJ09319207X (DE-599)DOAJef48172d98e34037a68c80853b61dd82
title_full	Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice
author_sort	Ping Li
journal	Agriculture
journalStr	Agriculture
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author_browse	Ping Li Nana Xu Yang Shui Jie Zhang Wuzhong Yin Min Tian Faping Guo Dasong Bai Pan Qi Qingxiong Huang Biluo Li Yuanyuan Li Yungao Hu Youlin Peng
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author-letter	Ping Li
doi_str_mv	10.3390/agriculture13101875
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title_sort	phenotypic analysis and gene cloning of a new allelic mutant of <i<spl5</i< in rice
callnumber	S1-972
title_auth	Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice
abstract	This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, <i<lm5</i< had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of <i<lm5</i<, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of <i<LOC_Os07g10390</i< (<i<OsLM5</i<) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The <i<OsLM5</i< gene was found to be a new allele of the <i<SPL5</i< gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and <i<OsLM5</i< was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant <i<lm5</i<, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The <i<OsLM5</i< gene may have a significant impact on the regulation of apoptosis in rice cells.
abstractGer	This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, <i<lm5</i< had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of <i<lm5</i<, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of <i<LOC_Os07g10390</i< (<i<OsLM5</i<) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The <i<OsLM5</i< gene was found to be a new allele of the <i<SPL5</i< gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and <i<OsLM5</i< was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant <i<lm5</i<, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The <i<OsLM5</i< gene may have a significant impact on the regulation of apoptosis in rice cells.
abstract_unstemmed	This study was conducted on the lesion-mimic mutant <i<lm5</i<, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant <i<lm5</i< was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, <i<lm5</i< had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of <i<lm5</i<, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of <i<LOC_Os07g10390</i< (<i<OsLM5</i<) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The <i<OsLM5</i< gene was found to be a new allele of the <i<SPL5</i< gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and <i<OsLM5</i< was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant <i<lm5</i<, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The <i<OsLM5</i< gene may have a significant impact on the regulation of apoptosis in rice cells.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700
container_issue	1875, p 1875
title_short	Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of <i<SPL5</i< in Rice
url	https://doi.org/10.3390/agriculture13101875 https://doaj.org/article/ef48172d98e34037a68c80853b61dd82 https://www.mdpi.com/2077-0472/13/10/1875 https://doaj.org/toc/2077-0472
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author2	Nana Xu Yang Shui Jie Zhang Wuzhong Yin Min Tian Faping Guo Dasong Bai Pan Qi Qingxiong Huang Biluo Li Yuanyuan Li Yungao Hu Youlin Peng
author2Str	Nana Xu Yang Shui Jie Zhang Wuzhong Yin Min Tian Faping Guo Dasong Bai Pan Qi Qingxiong Huang Biluo Li Yuanyuan Li Yungao Hu Youlin Peng
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up_date	2024-07-03T15:49:57.807Z
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