Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection

Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants fro...
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Autor*in:	Nathan A. Palmer [verfasserIn] Sophie Alvarez [verfasserIn] Michael J. Naldrett [verfasserIn] Anthony Muhle [verfasserIn] Gautam Sarath [verfasserIn] Serge J. Edmé [verfasserIn] Satyanarayana Tatineni [verfasserIn] Robert B. Mitchell [verfasserIn] Gary Yuen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	switchgrass rust LC-MS/MS plant defense

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 24(2023), 14630, p 14630
Übergeordnetes Werk:	volume:24 ; year:2023 ; number:14630, p 14630

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms241914630

Katalog-ID:	DOAJ093226861

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allfields	10.3390/ijms241914630 doi (DE-627)DOAJ093226861 (DE-599)DOAJ23a37299125047428de3ec4dd6578645 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Nathan A. Palmer verfasserin aut Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different <i<Puccinia</i< proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis (<i<A. thaliana</i< L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense. switchgrass <i<Panicum virgatum</i< rust <i<Puccinia novopanici</i< LC-MS/MS plant defense Biology (General) Chemistry Sophie Alvarez verfasserin aut Michael J. Naldrett verfasserin aut Anthony Muhle verfasserin aut Gautam Sarath verfasserin aut Serge J. Edmé verfasserin aut Satyanarayana Tatineni verfasserin aut Robert B. Mitchell verfasserin aut Gary Yuen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 14630, p 14630 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:14630, p 14630 https://doi.org/10.3390/ijms241914630 kostenfrei https://doaj.org/article/23a37299125047428de3ec4dd6578645 kostenfrei https://www.mdpi.com/1422-0067/24/19/14630 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 14630, p 14630
spelling	10.3390/ijms241914630 doi (DE-627)DOAJ093226861 (DE-599)DOAJ23a37299125047428de3ec4dd6578645 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Nathan A. Palmer verfasserin aut Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different <i<Puccinia</i< proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis (<i<A. thaliana</i< L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense. switchgrass <i<Panicum virgatum</i< rust <i<Puccinia novopanici</i< LC-MS/MS plant defense Biology (General) Chemistry Sophie Alvarez verfasserin aut Michael J. Naldrett verfasserin aut Anthony Muhle verfasserin aut Gautam Sarath verfasserin aut Serge J. Edmé verfasserin aut Satyanarayana Tatineni verfasserin aut Robert B. Mitchell verfasserin aut Gary Yuen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 14630, p 14630 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:14630, p 14630 https://doi.org/10.3390/ijms241914630 kostenfrei https://doaj.org/article/23a37299125047428de3ec4dd6578645 kostenfrei https://www.mdpi.com/1422-0067/24/19/14630 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 14630, p 14630
allfields_unstemmed	10.3390/ijms241914630 doi (DE-627)DOAJ093226861 (DE-599)DOAJ23a37299125047428de3ec4dd6578645 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Nathan A. Palmer verfasserin aut Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different <i<Puccinia</i< proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis (<i<A. thaliana</i< L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense. switchgrass <i<Panicum virgatum</i< rust <i<Puccinia novopanici</i< LC-MS/MS plant defense Biology (General) Chemistry Sophie Alvarez verfasserin aut Michael J. Naldrett verfasserin aut Anthony Muhle verfasserin aut Gautam Sarath verfasserin aut Serge J. Edmé verfasserin aut Satyanarayana Tatineni verfasserin aut Robert B. Mitchell verfasserin aut Gary Yuen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 14630, p 14630 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:14630, p 14630 https://doi.org/10.3390/ijms241914630 kostenfrei https://doaj.org/article/23a37299125047428de3ec4dd6578645 kostenfrei https://www.mdpi.com/1422-0067/24/19/14630 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 14630, p 14630
allfieldsGer	10.3390/ijms241914630 doi (DE-627)DOAJ093226861 (DE-599)DOAJ23a37299125047428de3ec4dd6578645 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Nathan A. Palmer verfasserin aut Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different <i<Puccinia</i< proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis (<i<A. thaliana</i< L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense. switchgrass <i<Panicum virgatum</i< rust <i<Puccinia novopanici</i< LC-MS/MS plant defense Biology (General) Chemistry Sophie Alvarez verfasserin aut Michael J. Naldrett verfasserin aut Anthony Muhle verfasserin aut Gautam Sarath verfasserin aut Serge J. Edmé verfasserin aut Satyanarayana Tatineni verfasserin aut Robert B. Mitchell verfasserin aut Gary Yuen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 14630, p 14630 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:14630, p 14630 https://doi.org/10.3390/ijms241914630 kostenfrei https://doaj.org/article/23a37299125047428de3ec4dd6578645 kostenfrei https://www.mdpi.com/1422-0067/24/19/14630 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 14630, p 14630
allfieldsSound	10.3390/ijms241914630 doi (DE-627)DOAJ093226861 (DE-599)DOAJ23a37299125047428de3ec4dd6578645 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Nathan A. Palmer verfasserin aut Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different <i<Puccinia</i< proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis (<i<A. thaliana</i< L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense. switchgrass <i<Panicum virgatum</i< rust <i<Puccinia novopanici</i< LC-MS/MS plant defense Biology (General) Chemistry Sophie Alvarez verfasserin aut Michael J. Naldrett verfasserin aut Anthony Muhle verfasserin aut Gautam Sarath verfasserin aut Serge J. Edmé verfasserin aut Satyanarayana Tatineni verfasserin aut Robert B. Mitchell verfasserin aut Gary Yuen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 14630, p 14630 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:14630, p 14630 https://doi.org/10.3390/ijms241914630 kostenfrei https://doaj.org/article/23a37299125047428de3ec4dd6578645 kostenfrei https://www.mdpi.com/1422-0067/24/19/14630 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 14630, p 14630
language	English
source	In International Journal of Molecular Sciences 24(2023), 14630, p 14630 volume:24 year:2023 number:14630, p 14630
sourceStr	In International Journal of Molecular Sciences 24(2023), 14630, p 14630 volume:24 year:2023 number:14630, p 14630
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	switchgrass <i<Panicum virgatum</i< rust <i<Puccinia novopanici</i< LC-MS/MS plant defense Biology (General) Chemistry
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container_title	International Journal of Molecular Sciences
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publishDateDaySort_date	2023-01-01T00:00:00Z
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callnumber-first	Q - Science
author	Nathan A. Palmer
spellingShingle	Nathan A. Palmer misc QH301-705.5 misc QD1-999 misc switchgrass misc <i<Panicum virgatum</i< misc rust misc <i<Puccinia novopanici</i< misc LC-MS/MS misc plant defense misc Biology (General) misc Chemistry Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection
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topic_title	QH301-705.5 QD1-999 Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection switchgrass <i<Panicum virgatum</i< rust <i<Puccinia novopanici</i< LC-MS/MS plant defense
topic	misc QH301-705.5 misc QD1-999 misc switchgrass misc <i<Panicum virgatum</i< misc rust misc <i<Puccinia novopanici</i< misc LC-MS/MS misc plant defense misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc switchgrass misc <i<Panicum virgatum</i< misc rust misc <i<Puccinia novopanici</i< misc LC-MS/MS misc plant defense misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc switchgrass misc <i<Panicum virgatum</i< misc rust misc <i<Puccinia novopanici</i< misc LC-MS/MS misc plant defense misc Biology (General) misc Chemistry
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title	Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection
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title_full	Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection
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author_browse	Nathan A. Palmer Sophie Alvarez Michael J. Naldrett Anthony Muhle Gautam Sarath Serge J. Edmé Satyanarayana Tatineni Robert B. Mitchell Gary Yuen
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title_sort	dynamic reconfiguration of switchgrass proteomes in response to rust (<i<puccinia novopanici</i<) infection
callnumber	QH301-705.5
title_auth	Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection
abstract	Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different <i<Puccinia</i< proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis (<i<A. thaliana</i< L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense.
abstractGer	Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different <i<Puccinia</i< proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis (<i<A. thaliana</i< L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense.
abstract_unstemmed	Switchgrass (<i<Panicum virgatum</i< L.) can be infected by the rust pathogen (<i<Puccinia novopanici</i<) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different <i<Puccinia</i< proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis (<i<A. thaliana</i< L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense.
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title_short	Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (<i<Puccinia novopanici</i<) Infection
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