Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation
Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule det...
Ausführliche Beschreibung
Autor*in: |
Lulu Wang [verfasserIn] Yan Sun [verfasserIn] Ruihao Zhang [verfasserIn] Kehou Pan [verfasserIn] Yuhang Li [verfasserIn] Ruibing Wang [verfasserIn] Lin Zhang [verfasserIn] Chengxu Zhou [verfasserIn] Jian Li [verfasserIn] Yun Li [verfasserIn] Baohua Zhu [verfasserIn] Jichang Han [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2023 |
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In: Biotechnology for Biofuels and Bioproducts - BMC, 2022, 16(2023), 1, Seite 21 |
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Übergeordnetes Werk: |
volume:16 ; year:2023 ; number:1 ; pages:21 |
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DOI / URN: |
10.1186/s13068-023-02389-x |
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Katalog-ID: |
DOAJ093300042 |
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520 | |a Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. | ||
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10.1186/s13068-023-02389-x doi (DE-627)DOAJ093300042 (DE-599)DOAJ9f790f8f525c45b4b2512eb2832176fc DE-627 ger DE-627 rakwb eng TP248.13-248.65 TP315-360 Lulu Wang verfasserin aut Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. Diatom frustule Silica Morphology remolding Genetic modification Hemostatic material Biotechnology Fuel Yan Sun verfasserin aut Ruihao Zhang verfasserin aut Kehou Pan verfasserin aut Yuhang Li verfasserin aut Ruibing Wang verfasserin aut Lin Zhang verfasserin aut Chengxu Zhou verfasserin aut Jian Li verfasserin aut Yun Li verfasserin aut Baohua Zhu verfasserin aut Jichang Han verfasserin aut In Biotechnology for Biofuels and Bioproducts BMC, 2022 16(2023), 1, Seite 21 (DE-627)1787064794 27313654 nnns volume:16 year:2023 number:1 pages:21 https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/article/9f790f8f525c45b4b2512eb2832176fc kostenfrei https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/toc/2731-3654 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2023 1 21 |
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10.1186/s13068-023-02389-x doi (DE-627)DOAJ093300042 (DE-599)DOAJ9f790f8f525c45b4b2512eb2832176fc DE-627 ger DE-627 rakwb eng TP248.13-248.65 TP315-360 Lulu Wang verfasserin aut Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. Diatom frustule Silica Morphology remolding Genetic modification Hemostatic material Biotechnology Fuel Yan Sun verfasserin aut Ruihao Zhang verfasserin aut Kehou Pan verfasserin aut Yuhang Li verfasserin aut Ruibing Wang verfasserin aut Lin Zhang verfasserin aut Chengxu Zhou verfasserin aut Jian Li verfasserin aut Yun Li verfasserin aut Baohua Zhu verfasserin aut Jichang Han verfasserin aut In Biotechnology for Biofuels and Bioproducts BMC, 2022 16(2023), 1, Seite 21 (DE-627)1787064794 27313654 nnns volume:16 year:2023 number:1 pages:21 https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/article/9f790f8f525c45b4b2512eb2832176fc kostenfrei https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/toc/2731-3654 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2023 1 21 |
allfields_unstemmed |
10.1186/s13068-023-02389-x doi (DE-627)DOAJ093300042 (DE-599)DOAJ9f790f8f525c45b4b2512eb2832176fc DE-627 ger DE-627 rakwb eng TP248.13-248.65 TP315-360 Lulu Wang verfasserin aut Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. Diatom frustule Silica Morphology remolding Genetic modification Hemostatic material Biotechnology Fuel Yan Sun verfasserin aut Ruihao Zhang verfasserin aut Kehou Pan verfasserin aut Yuhang Li verfasserin aut Ruibing Wang verfasserin aut Lin Zhang verfasserin aut Chengxu Zhou verfasserin aut Jian Li verfasserin aut Yun Li verfasserin aut Baohua Zhu verfasserin aut Jichang Han verfasserin aut In Biotechnology for Biofuels and Bioproducts BMC, 2022 16(2023), 1, Seite 21 (DE-627)1787064794 27313654 nnns volume:16 year:2023 number:1 pages:21 https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/article/9f790f8f525c45b4b2512eb2832176fc kostenfrei https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/toc/2731-3654 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2023 1 21 |
allfieldsGer |
10.1186/s13068-023-02389-x doi (DE-627)DOAJ093300042 (DE-599)DOAJ9f790f8f525c45b4b2512eb2832176fc DE-627 ger DE-627 rakwb eng TP248.13-248.65 TP315-360 Lulu Wang verfasserin aut Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. Diatom frustule Silica Morphology remolding Genetic modification Hemostatic material Biotechnology Fuel Yan Sun verfasserin aut Ruihao Zhang verfasserin aut Kehou Pan verfasserin aut Yuhang Li verfasserin aut Ruibing Wang verfasserin aut Lin Zhang verfasserin aut Chengxu Zhou verfasserin aut Jian Li verfasserin aut Yun Li verfasserin aut Baohua Zhu verfasserin aut Jichang Han verfasserin aut In Biotechnology for Biofuels and Bioproducts BMC, 2022 16(2023), 1, Seite 21 (DE-627)1787064794 27313654 nnns volume:16 year:2023 number:1 pages:21 https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/article/9f790f8f525c45b4b2512eb2832176fc kostenfrei https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/toc/2731-3654 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2023 1 21 |
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10.1186/s13068-023-02389-x doi (DE-627)DOAJ093300042 (DE-599)DOAJ9f790f8f525c45b4b2512eb2832176fc DE-627 ger DE-627 rakwb eng TP248.13-248.65 TP315-360 Lulu Wang verfasserin aut Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. Diatom frustule Silica Morphology remolding Genetic modification Hemostatic material Biotechnology Fuel Yan Sun verfasserin aut Ruihao Zhang verfasserin aut Kehou Pan verfasserin aut Yuhang Li verfasserin aut Ruibing Wang verfasserin aut Lin Zhang verfasserin aut Chengxu Zhou verfasserin aut Jian Li verfasserin aut Yun Li verfasserin aut Baohua Zhu verfasserin aut Jichang Han verfasserin aut In Biotechnology for Biofuels and Bioproducts BMC, 2022 16(2023), 1, Seite 21 (DE-627)1787064794 27313654 nnns volume:16 year:2023 number:1 pages:21 https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/article/9f790f8f525c45b4b2512eb2832176fc kostenfrei https://doi.org/10.1186/s13068-023-02389-x kostenfrei https://doaj.org/toc/2731-3654 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2023 1 21 |
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enhancement of hemostatic properties of cyclotella cryptica frustule through genetic manipulation |
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Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation |
abstract |
Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. |
abstractGer |
Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. |
abstract_unstemmed |
Abstract Background The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, and ready availability. As the architectural features of the frustule determine its hemostatic performance, it is of great interest to develop an effective method to modify the frustule morphology into desired patterns to further improve hemostatic efficiency. Results In this study, the gene encoding Silicalemma Associated Protein 2 (a silicalemma-spanning protein) of Cyclotella cryptica (CcSAP2) was identified as a key gene in frustule morphogenesis. Thus, it was overexpressed and knocked down, respectively. The frustule of the overexpress lines showed no obvious alteration in morphology compared to the wild type (WT), while the size, specific surface area (BET), pore volume, and pore diameter of the knockdown strains changed greatly. Particularly, the knockdown frustules achieved a more pronounced coagulation effect and in vivo hemostatic performance than the WT strains. Such observations suggested that silicalemma proteins are ideal genetic encoding targets for manipulating frustule morphology associated hemostatic properties. Furthermore, the Mantel test was adopted to identify the key morphologies associated with C. cryptica bleeding control. Finally, based on our results and recent advances, the mechanism of frustule morphogenesis was discussed. Conclusion This study explores a new strategy for enhancing the hemostatic efficiency of the frustule based on genetic morphology modification and may provide insights into a better understanding of the frustule morphogenesis mechanism. |
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Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation |
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https://doi.org/10.1186/s13068-023-02389-x https://doaj.org/article/9f790f8f525c45b4b2512eb2832176fc https://doaj.org/toc/2731-3654 |
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Yan Sun Ruihao Zhang Kehou Pan Yuhang Li Ruibing Wang Lin Zhang Chengxu Zhou Jian Li Yun Li Baohua Zhu Jichang Han |
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Yan Sun Ruihao Zhang Kehou Pan Yuhang Li Ruibing Wang Lin Zhang Chengxu Zhou Jian Li Yun Li Baohua Zhu Jichang Han |
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