Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize
Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought st...
Ausführliche Beschreibung
Autor*in: |
Pengyu Zhang [verfasserIn] Tongchao Wang [verfasserIn] Liru Cao [verfasserIn] Zhixin Jiao [verfasserIn] Lixia Ku [verfasserIn] Dandan Dou [verfasserIn] Zhixue Liu [verfasserIn] Jiaxu Fu [verfasserIn] Xiaowen Xie [verfasserIn] Yingfang Zhu [verfasserIn] Leelyn Chong [verfasserIn] Li Wei [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2023 |
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Übergeordnetes Werk: |
In: Stress Biology - Springer, 2022, 3(2023), 1, Seite 15 |
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Übergeordnetes Werk: |
volume:3 ; year:2023 ; number:1 ; pages:15 |
Links: |
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DOI / URN: |
10.1007/s44154-023-00125-x |
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Katalog-ID: |
DOAJ093321384 |
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520 | |a Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. | ||
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10.1007/s44154-023-00125-x doi (DE-627)DOAJ093321384 (DE-599)DOAJa2321682af8d40659d3d0be20a0602a9 DE-627 ger DE-627 rakwb eng QH301-705.5 Pengyu Zhang verfasserin aut Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. ZmRL6 1R-MYB MYB-related genes Drought stress Maize Biology (General) Tongchao Wang verfasserin aut Liru Cao verfasserin aut Zhixin Jiao verfasserin aut Lixia Ku verfasserin aut Dandan Dou verfasserin aut Zhixue Liu verfasserin aut Jiaxu Fu verfasserin aut Xiaowen Xie verfasserin aut Yingfang Zhu verfasserin aut Leelyn Chong verfasserin aut Li Wei verfasserin aut In Stress Biology Springer, 2022 3(2023), 1, Seite 15 (DE-627)1770765034 27310450 nnns volume:3 year:2023 number:1 pages:15 https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/article/a2321682af8d40659d3d0be20a0602a9 kostenfrei https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/toc/2731-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2023 1 15 |
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10.1007/s44154-023-00125-x doi (DE-627)DOAJ093321384 (DE-599)DOAJa2321682af8d40659d3d0be20a0602a9 DE-627 ger DE-627 rakwb eng QH301-705.5 Pengyu Zhang verfasserin aut Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. ZmRL6 1R-MYB MYB-related genes Drought stress Maize Biology (General) Tongchao Wang verfasserin aut Liru Cao verfasserin aut Zhixin Jiao verfasserin aut Lixia Ku verfasserin aut Dandan Dou verfasserin aut Zhixue Liu verfasserin aut Jiaxu Fu verfasserin aut Xiaowen Xie verfasserin aut Yingfang Zhu verfasserin aut Leelyn Chong verfasserin aut Li Wei verfasserin aut In Stress Biology Springer, 2022 3(2023), 1, Seite 15 (DE-627)1770765034 27310450 nnns volume:3 year:2023 number:1 pages:15 https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/article/a2321682af8d40659d3d0be20a0602a9 kostenfrei https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/toc/2731-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2023 1 15 |
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10.1007/s44154-023-00125-x doi (DE-627)DOAJ093321384 (DE-599)DOAJa2321682af8d40659d3d0be20a0602a9 DE-627 ger DE-627 rakwb eng QH301-705.5 Pengyu Zhang verfasserin aut Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. ZmRL6 1R-MYB MYB-related genes Drought stress Maize Biology (General) Tongchao Wang verfasserin aut Liru Cao verfasserin aut Zhixin Jiao verfasserin aut Lixia Ku verfasserin aut Dandan Dou verfasserin aut Zhixue Liu verfasserin aut Jiaxu Fu verfasserin aut Xiaowen Xie verfasserin aut Yingfang Zhu verfasserin aut Leelyn Chong verfasserin aut Li Wei verfasserin aut In Stress Biology Springer, 2022 3(2023), 1, Seite 15 (DE-627)1770765034 27310450 nnns volume:3 year:2023 number:1 pages:15 https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/article/a2321682af8d40659d3d0be20a0602a9 kostenfrei https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/toc/2731-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2023 1 15 |
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10.1007/s44154-023-00125-x doi (DE-627)DOAJ093321384 (DE-599)DOAJa2321682af8d40659d3d0be20a0602a9 DE-627 ger DE-627 rakwb eng QH301-705.5 Pengyu Zhang verfasserin aut Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. ZmRL6 1R-MYB MYB-related genes Drought stress Maize Biology (General) Tongchao Wang verfasserin aut Liru Cao verfasserin aut Zhixin Jiao verfasserin aut Lixia Ku verfasserin aut Dandan Dou verfasserin aut Zhixue Liu verfasserin aut Jiaxu Fu verfasserin aut Xiaowen Xie verfasserin aut Yingfang Zhu verfasserin aut Leelyn Chong verfasserin aut Li Wei verfasserin aut In Stress Biology Springer, 2022 3(2023), 1, Seite 15 (DE-627)1770765034 27310450 nnns volume:3 year:2023 number:1 pages:15 https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/article/a2321682af8d40659d3d0be20a0602a9 kostenfrei https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/toc/2731-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2023 1 15 |
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10.1007/s44154-023-00125-x doi (DE-627)DOAJ093321384 (DE-599)DOAJa2321682af8d40659d3d0be20a0602a9 DE-627 ger DE-627 rakwb eng QH301-705.5 Pengyu Zhang verfasserin aut Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. ZmRL6 1R-MYB MYB-related genes Drought stress Maize Biology (General) Tongchao Wang verfasserin aut Liru Cao verfasserin aut Zhixin Jiao verfasserin aut Lixia Ku verfasserin aut Dandan Dou verfasserin aut Zhixue Liu verfasserin aut Jiaxu Fu verfasserin aut Xiaowen Xie verfasserin aut Yingfang Zhu verfasserin aut Leelyn Chong verfasserin aut Li Wei verfasserin aut In Stress Biology Springer, 2022 3(2023), 1, Seite 15 (DE-627)1770765034 27310450 nnns volume:3 year:2023 number:1 pages:15 https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/article/a2321682af8d40659d3d0be20a0602a9 kostenfrei https://doi.org/10.1007/s44154-023-00125-x kostenfrei https://doaj.org/toc/2731-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2023 1 15 |
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Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. |
abstractGer |
Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. |
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Abstract MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize’s drought response. |
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