Tetraploidization Increases the Motility and Invasiveness of Cancer Cells

Polyploidy and metastasis are associated with a low probability of disease-free survival in cancer patients. Polyploid cells are known to facilitate tumorigenesis. However, few data associate polyploidization with metastasis. Here, by generating and using diploid (2n) and tetraploid (4n) clones from...
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Gespeichert in:

Autor*in:	Mohamed Jemaà [verfasserIn] Renee Daams [verfasserIn] Slim Charfi [verfasserIn] Fredrik Mertens [verfasserIn] Stephan M. Huber [verfasserIn] Ramin Massoumi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	colon cancer sarcoma tetraploidy migration invasion metastasis

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 24(2023), 13926, p 13926
Übergeordnetes Werk:	volume:24 ; year:2023 ; number:13926, p 13926

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms241813926

Katalog-ID:	DOAJ093389892

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spelling	10.3390/ijms241813926 doi (DE-627)DOAJ093389892 (DE-599)DOAJ4d1fb7fab8d0448ab948378562ee5f83 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mohamed Jemaà verfasserin aut Tetraploidization Increases the Motility and Invasiveness of Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyploidy and metastasis are associated with a low probability of disease-free survival in cancer patients. Polyploid cells are known to facilitate tumorigenesis. However, few data associate polyploidization with metastasis. Here, by generating and using diploid (2n) and tetraploid (4n) clones from malignant fibrous histiocytoma (MFH) and colon carcinoma (RKO), we demonstrate the migration and invasion advantage of tetraploid cells in vitro using several assays, including the wound healing, the OrisTM two-dimensional cell migration, single-cell migration tracking by video microscopy, the Boyden chamber, and the xCELLigence RTCA real-time cell migration. Motility advantage was observed despite tetraploid cell proliferation weakness. We could also demonstrate preferential metastatic potential in vivo for the tetraploid clone using the tail vein injection in mice and tracking metastatic tumors in the lung. Using the Mitelman Database of Chromosome Aberrations in Cancer, we found an accumulation of polyploid karyotypes in metastatic tumors compared to primary ones. This work reveals the clinical relevance of the polyploid subpopulation and the strategic need to highlight polyploidy in preclinical studies as a therapeutic target for metastasis. colon cancer sarcoma tetraploidy migration invasion metastasis Biology (General) Chemistry Renee Daams verfasserin aut Slim Charfi verfasserin aut Fredrik Mertens verfasserin aut Stephan M. Huber verfasserin aut Ramin Massoumi verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 13926, p 13926 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:13926, p 13926 https://doi.org/10.3390/ijms241813926 kostenfrei https://doaj.org/article/4d1fb7fab8d0448ab948378562ee5f83 kostenfrei https://www.mdpi.com/1422-0067/24/18/13926 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 13926, p 13926
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allfieldsSound	10.3390/ijms241813926 doi (DE-627)DOAJ093389892 (DE-599)DOAJ4d1fb7fab8d0448ab948378562ee5f83 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mohamed Jemaà verfasserin aut Tetraploidization Increases the Motility and Invasiveness of Cancer Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyploidy and metastasis are associated with a low probability of disease-free survival in cancer patients. Polyploid cells are known to facilitate tumorigenesis. However, few data associate polyploidization with metastasis. Here, by generating and using diploid (2n) and tetraploid (4n) clones from malignant fibrous histiocytoma (MFH) and colon carcinoma (RKO), we demonstrate the migration and invasion advantage of tetraploid cells in vitro using several assays, including the wound healing, the OrisTM two-dimensional cell migration, single-cell migration tracking by video microscopy, the Boyden chamber, and the xCELLigence RTCA real-time cell migration. Motility advantage was observed despite tetraploid cell proliferation weakness. We could also demonstrate preferential metastatic potential in vivo for the tetraploid clone using the tail vein injection in mice and tracking metastatic tumors in the lung. Using the Mitelman Database of Chromosome Aberrations in Cancer, we found an accumulation of polyploid karyotypes in metastatic tumors compared to primary ones. This work reveals the clinical relevance of the polyploid subpopulation and the strategic need to highlight polyploidy in preclinical studies as a therapeutic target for metastasis. colon cancer sarcoma tetraploidy migration invasion metastasis Biology (General) Chemistry Renee Daams verfasserin aut Slim Charfi verfasserin aut Fredrik Mertens verfasserin aut Stephan M. Huber verfasserin aut Ramin Massoumi verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 13926, p 13926 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:13926, p 13926 https://doi.org/10.3390/ijms241813926 kostenfrei https://doaj.org/article/4d1fb7fab8d0448ab948378562ee5f83 kostenfrei https://www.mdpi.com/1422-0067/24/18/13926 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 13926, p 13926
language	English
source	In International Journal of Molecular Sciences 24(2023), 13926, p 13926 volume:24 year:2023 number:13926, p 13926
sourceStr	In International Journal of Molecular Sciences 24(2023), 13926, p 13926 volume:24 year:2023 number:13926, p 13926
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	colon cancer sarcoma tetraploidy migration invasion metastasis Biology (General) Chemistry
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container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Mohamed Jemaà @@aut@@ Renee Daams @@aut@@ Slim Charfi @@aut@@ Fredrik Mertens @@aut@@ Stephan M. Huber @@aut@@ Ramin Massoumi @@aut@@
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author	Mohamed Jemaà
spellingShingle	Mohamed Jemaà misc QH301-705.5 misc QD1-999 misc colon cancer misc sarcoma misc tetraploidy misc migration misc invasion misc metastasis misc Biology (General) misc Chemistry Tetraploidization Increases the Motility and Invasiveness of Cancer Cells
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topic_title	QH301-705.5 QD1-999 Tetraploidization Increases the Motility and Invasiveness of Cancer Cells colon cancer sarcoma tetraploidy migration invasion metastasis
topic	misc QH301-705.5 misc QD1-999 misc colon cancer misc sarcoma misc tetraploidy misc migration misc invasion misc metastasis misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc colon cancer misc sarcoma misc tetraploidy misc migration misc invasion misc metastasis misc Biology (General) misc Chemistry
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title_auth	Tetraploidization Increases the Motility and Invasiveness of Cancer Cells
abstract	Polyploidy and metastasis are associated with a low probability of disease-free survival in cancer patients. Polyploid cells are known to facilitate tumorigenesis. However, few data associate polyploidization with metastasis. Here, by generating and using diploid (2n) and tetraploid (4n) clones from malignant fibrous histiocytoma (MFH) and colon carcinoma (RKO), we demonstrate the migration and invasion advantage of tetraploid cells in vitro using several assays, including the wound healing, the OrisTM two-dimensional cell migration, single-cell migration tracking by video microscopy, the Boyden chamber, and the xCELLigence RTCA real-time cell migration. Motility advantage was observed despite tetraploid cell proliferation weakness. We could also demonstrate preferential metastatic potential in vivo for the tetraploid clone using the tail vein injection in mice and tracking metastatic tumors in the lung. Using the Mitelman Database of Chromosome Aberrations in Cancer, we found an accumulation of polyploid karyotypes in metastatic tumors compared to primary ones. This work reveals the clinical relevance of the polyploid subpopulation and the strategic need to highlight polyploidy in preclinical studies as a therapeutic target for metastasis.
abstractGer	Polyploidy and metastasis are associated with a low probability of disease-free survival in cancer patients. Polyploid cells are known to facilitate tumorigenesis. However, few data associate polyploidization with metastasis. Here, by generating and using diploid (2n) and tetraploid (4n) clones from malignant fibrous histiocytoma (MFH) and colon carcinoma (RKO), we demonstrate the migration and invasion advantage of tetraploid cells in vitro using several assays, including the wound healing, the OrisTM two-dimensional cell migration, single-cell migration tracking by video microscopy, the Boyden chamber, and the xCELLigence RTCA real-time cell migration. Motility advantage was observed despite tetraploid cell proliferation weakness. We could also demonstrate preferential metastatic potential in vivo for the tetraploid clone using the tail vein injection in mice and tracking metastatic tumors in the lung. Using the Mitelman Database of Chromosome Aberrations in Cancer, we found an accumulation of polyploid karyotypes in metastatic tumors compared to primary ones. This work reveals the clinical relevance of the polyploid subpopulation and the strategic need to highlight polyploidy in preclinical studies as a therapeutic target for metastasis.
abstract_unstemmed	Polyploidy and metastasis are associated with a low probability of disease-free survival in cancer patients. Polyploid cells are known to facilitate tumorigenesis. However, few data associate polyploidization with metastasis. Here, by generating and using diploid (2n) and tetraploid (4n) clones from malignant fibrous histiocytoma (MFH) and colon carcinoma (RKO), we demonstrate the migration and invasion advantage of tetraploid cells in vitro using several assays, including the wound healing, the OrisTM two-dimensional cell migration, single-cell migration tracking by video microscopy, the Boyden chamber, and the xCELLigence RTCA real-time cell migration. Motility advantage was observed despite tetraploid cell proliferation weakness. We could also demonstrate preferential metastatic potential in vivo for the tetraploid clone using the tail vein injection in mice and tracking metastatic tumors in the lung. Using the Mitelman Database of Chromosome Aberrations in Cancer, we found an accumulation of polyploid karyotypes in metastatic tumors compared to primary ones. This work reveals the clinical relevance of the polyploid subpopulation and the strategic need to highlight polyploidy in preclinical studies as a therapeutic target for metastasis.
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title_short	Tetraploidization Increases the Motility and Invasiveness of Cancer Cells
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Polyploid cells are known to facilitate tumorigenesis. However, few data associate polyploidization with metastasis. Here, by generating and using diploid (2n) and tetraploid (4n) clones from malignant fibrous histiocytoma (MFH) and colon carcinoma (RKO), we demonstrate the migration and invasion advantage of tetraploid cells in vitro using several assays, including the wound healing, the OrisTM two-dimensional cell migration, single-cell migration tracking by video microscopy, the Boyden chamber, and the xCELLigence RTCA real-time cell migration. Motility advantage was observed despite tetraploid cell proliferation weakness. We could also demonstrate preferential metastatic potential in vivo for the tetraploid clone using the tail vein injection in mice and tracking metastatic tumors in the lung. Using the Mitelman Database of Chromosome Aberrations in Cancer, we found an accumulation of polyploid karyotypes in metastatic tumors compared to primary ones. 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Tetraploidization Increases the Motility and Invasiveness of Cancer Cells

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