ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma

We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma...
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Autor*in:	Shohei Sekiguchi [verfasserIn] Akira Yorozu [verfasserIn] Fumika Okazaki [verfasserIn] Takeshi Niinuma [verfasserIn] Akira Takasawa [verfasserIn] Eiichiro Yamamoto [verfasserIn] Hiroshi Kitajima [verfasserIn] Toshiyuki Kubo [verfasserIn] Yui Hatanaka [verfasserIn] Koyo Nishiyama [verfasserIn] Kazuhiro Ogi [verfasserIn] Hironari Dehari [verfasserIn] Atsushi Kondo [verfasserIn] Makoto Kurose [verfasserIn] Kazufumi Obata [verfasserIn] Akito Kakiuchi [verfasserIn] Masahiro Kai [verfasserIn] Yoshihiko Hirohashi [verfasserIn] Toshihiko Torigoe [verfasserIn] Takashi Kojima [verfasserIn] Makoto Osanai [verfasserIn] Kenichi Takano [verfasserIn] Akihiro Miyazaki [verfasserIn] Hiromu Suzuki [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	oral cancer tumor microenvironment cancer-associated fibroblast collagen tumor-infiltrating lymphocyte

Übergeordnetes Werk:	In: Cancers - MDPI AG, 2010, 15(2023), 17, p 4303
Übergeordnetes Werk:	volume:15 ; year:2023 ; number:17, p 4303

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cancers15174303

Katalog-ID:	DOAJ093526822

Internformat


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520			\|a We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target.
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allfields	10.3390/cancers15174303 doi (DE-627)DOAJ093526822 (DE-599)DOAJ3bd23d4ebe7c4bc39e65413a25780fe0 DE-627 ger DE-627 rakwb eng RC254-282 Shohei Sekiguchi verfasserin aut ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target. oral cancer tumor microenvironment cancer-associated fibroblast collagen tumor-infiltrating lymphocyte Neoplasms. Tumors. Oncology. Including cancer and carcinogens Akira Yorozu verfasserin aut Fumika Okazaki verfasserin aut Takeshi Niinuma verfasserin aut Akira Takasawa verfasserin aut Eiichiro Yamamoto verfasserin aut Hiroshi Kitajima verfasserin aut Toshiyuki Kubo verfasserin aut Yui Hatanaka verfasserin aut Koyo Nishiyama verfasserin aut Kazuhiro Ogi verfasserin aut Hironari Dehari verfasserin aut Atsushi Kondo verfasserin aut Makoto Kurose verfasserin aut Kazufumi Obata verfasserin aut Akito Kakiuchi verfasserin aut Masahiro Kai verfasserin aut Yoshihiko Hirohashi verfasserin aut Toshihiko Torigoe verfasserin aut Takashi Kojima verfasserin aut Makoto Osanai verfasserin aut Kenichi Takano verfasserin aut Akihiro Miyazaki verfasserin aut Hiromu Suzuki verfasserin aut In Cancers MDPI AG, 2010 15(2023), 17, p 4303 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:15 year:2023 number:17, p 4303 https://doi.org/10.3390/cancers15174303 kostenfrei https://doaj.org/article/3bd23d4ebe7c4bc39e65413a25780fe0 kostenfrei https://www.mdpi.com/2072-6694/15/17/4303 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2023 17, p 4303
spelling	10.3390/cancers15174303 doi (DE-627)DOAJ093526822 (DE-599)DOAJ3bd23d4ebe7c4bc39e65413a25780fe0 DE-627 ger DE-627 rakwb eng RC254-282 Shohei Sekiguchi verfasserin aut ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target. oral cancer tumor microenvironment cancer-associated fibroblast collagen tumor-infiltrating lymphocyte Neoplasms. Tumors. Oncology. Including cancer and carcinogens Akira Yorozu verfasserin aut Fumika Okazaki verfasserin aut Takeshi Niinuma verfasserin aut Akira Takasawa verfasserin aut Eiichiro Yamamoto verfasserin aut Hiroshi Kitajima verfasserin aut Toshiyuki Kubo verfasserin aut Yui Hatanaka verfasserin aut Koyo Nishiyama verfasserin aut Kazuhiro Ogi verfasserin aut Hironari Dehari verfasserin aut Atsushi Kondo verfasserin aut Makoto Kurose verfasserin aut Kazufumi Obata verfasserin aut Akito Kakiuchi verfasserin aut Masahiro Kai verfasserin aut Yoshihiko Hirohashi verfasserin aut Toshihiko Torigoe verfasserin aut Takashi Kojima verfasserin aut Makoto Osanai verfasserin aut Kenichi Takano verfasserin aut Akihiro Miyazaki verfasserin aut Hiromu Suzuki verfasserin aut In Cancers MDPI AG, 2010 15(2023), 17, p 4303 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:15 year:2023 number:17, p 4303 https://doi.org/10.3390/cancers15174303 kostenfrei https://doaj.org/article/3bd23d4ebe7c4bc39e65413a25780fe0 kostenfrei https://www.mdpi.com/2072-6694/15/17/4303 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2023 17, p 4303
allfields_unstemmed	10.3390/cancers15174303 doi (DE-627)DOAJ093526822 (DE-599)DOAJ3bd23d4ebe7c4bc39e65413a25780fe0 DE-627 ger DE-627 rakwb eng RC254-282 Shohei Sekiguchi verfasserin aut ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target. oral cancer tumor microenvironment cancer-associated fibroblast collagen tumor-infiltrating lymphocyte Neoplasms. Tumors. Oncology. Including cancer and carcinogens Akira Yorozu verfasserin aut Fumika Okazaki verfasserin aut Takeshi Niinuma verfasserin aut Akira Takasawa verfasserin aut Eiichiro Yamamoto verfasserin aut Hiroshi Kitajima verfasserin aut Toshiyuki Kubo verfasserin aut Yui Hatanaka verfasserin aut Koyo Nishiyama verfasserin aut Kazuhiro Ogi verfasserin aut Hironari Dehari verfasserin aut Atsushi Kondo verfasserin aut Makoto Kurose verfasserin aut Kazufumi Obata verfasserin aut Akito Kakiuchi verfasserin aut Masahiro Kai verfasserin aut Yoshihiko Hirohashi verfasserin aut Toshihiko Torigoe verfasserin aut Takashi Kojima verfasserin aut Makoto Osanai verfasserin aut Kenichi Takano verfasserin aut Akihiro Miyazaki verfasserin aut Hiromu Suzuki verfasserin aut In Cancers MDPI AG, 2010 15(2023), 17, p 4303 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:15 year:2023 number:17, p 4303 https://doi.org/10.3390/cancers15174303 kostenfrei https://doaj.org/article/3bd23d4ebe7c4bc39e65413a25780fe0 kostenfrei https://www.mdpi.com/2072-6694/15/17/4303 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2023 17, p 4303
allfieldsGer	10.3390/cancers15174303 doi (DE-627)DOAJ093526822 (DE-599)DOAJ3bd23d4ebe7c4bc39e65413a25780fe0 DE-627 ger DE-627 rakwb eng RC254-282 Shohei Sekiguchi verfasserin aut ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target. oral cancer tumor microenvironment cancer-associated fibroblast collagen tumor-infiltrating lymphocyte Neoplasms. Tumors. Oncology. Including cancer and carcinogens Akira Yorozu verfasserin aut Fumika Okazaki verfasserin aut Takeshi Niinuma verfasserin aut Akira Takasawa verfasserin aut Eiichiro Yamamoto verfasserin aut Hiroshi Kitajima verfasserin aut Toshiyuki Kubo verfasserin aut Yui Hatanaka verfasserin aut Koyo Nishiyama verfasserin aut Kazuhiro Ogi verfasserin aut Hironari Dehari verfasserin aut Atsushi Kondo verfasserin aut Makoto Kurose verfasserin aut Kazufumi Obata verfasserin aut Akito Kakiuchi verfasserin aut Masahiro Kai verfasserin aut Yoshihiko Hirohashi verfasserin aut Toshihiko Torigoe verfasserin aut Takashi Kojima verfasserin aut Makoto Osanai verfasserin aut Kenichi Takano verfasserin aut Akihiro Miyazaki verfasserin aut Hiromu Suzuki verfasserin aut In Cancers MDPI AG, 2010 15(2023), 17, p 4303 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:15 year:2023 number:17, p 4303 https://doi.org/10.3390/cancers15174303 kostenfrei https://doaj.org/article/3bd23d4ebe7c4bc39e65413a25780fe0 kostenfrei https://www.mdpi.com/2072-6694/15/17/4303 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2023 17, p 4303
allfieldsSound	10.3390/cancers15174303 doi (DE-627)DOAJ093526822 (DE-599)DOAJ3bd23d4ebe7c4bc39e65413a25780fe0 DE-627 ger DE-627 rakwb eng RC254-282 Shohei Sekiguchi verfasserin aut ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target. oral cancer tumor microenvironment cancer-associated fibroblast collagen tumor-infiltrating lymphocyte Neoplasms. Tumors. Oncology. Including cancer and carcinogens Akira Yorozu verfasserin aut Fumika Okazaki verfasserin aut Takeshi Niinuma verfasserin aut Akira Takasawa verfasserin aut Eiichiro Yamamoto verfasserin aut Hiroshi Kitajima verfasserin aut Toshiyuki Kubo verfasserin aut Yui Hatanaka verfasserin aut Koyo Nishiyama verfasserin aut Kazuhiro Ogi verfasserin aut Hironari Dehari verfasserin aut Atsushi Kondo verfasserin aut Makoto Kurose verfasserin aut Kazufumi Obata verfasserin aut Akito Kakiuchi verfasserin aut Masahiro Kai verfasserin aut Yoshihiko Hirohashi verfasserin aut Toshihiko Torigoe verfasserin aut Takashi Kojima verfasserin aut Makoto Osanai verfasserin aut Kenichi Takano verfasserin aut Akihiro Miyazaki verfasserin aut Hiromu Suzuki verfasserin aut In Cancers MDPI AG, 2010 15(2023), 17, p 4303 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:15 year:2023 number:17, p 4303 https://doi.org/10.3390/cancers15174303 kostenfrei https://doaj.org/article/3bd23d4ebe7c4bc39e65413a25780fe0 kostenfrei https://www.mdpi.com/2072-6694/15/17/4303 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2023 17, p 4303
language	English
source	In Cancers 15(2023), 17, p 4303 volume:15 year:2023 number:17, p 4303
sourceStr	In Cancers 15(2023), 17, p 4303 volume:15 year:2023 number:17, p 4303
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	oral cancer tumor microenvironment cancer-associated fibroblast collagen tumor-infiltrating lymphocyte Neoplasms. Tumors. Oncology. Including cancer and carcinogens
isfreeaccess_bool	true
container_title	Cancers
authorswithroles_txt_mv	Shohei Sekiguchi @@aut@@ Akira Yorozu @@aut@@ Fumika Okazaki @@aut@@ Takeshi Niinuma @@aut@@ Akira Takasawa @@aut@@ Eiichiro Yamamoto @@aut@@ Hiroshi Kitajima @@aut@@ Toshiyuki Kubo @@aut@@ Yui Hatanaka @@aut@@ Koyo Nishiyama @@aut@@ Kazuhiro Ogi @@aut@@ Hironari Dehari @@aut@@ Atsushi Kondo @@aut@@ Makoto Kurose @@aut@@ Kazufumi Obata @@aut@@ Akito Kakiuchi @@aut@@ Masahiro Kai @@aut@@ Yoshihiko Hirohashi @@aut@@ Toshihiko Torigoe @@aut@@ Takashi Kojima @@aut@@ Makoto Osanai @@aut@@ Kenichi Takano @@aut@@ Akihiro Miyazaki @@aut@@ Hiromu Suzuki @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	614095670
id	DOAJ093526822
language_de	englisch
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callnumber-first	R - Medicine
author	Shohei Sekiguchi
spellingShingle	Shohei Sekiguchi misc RC254-282 misc oral cancer misc tumor microenvironment misc cancer-associated fibroblast misc collagen misc tumor-infiltrating lymphocyte misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma
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topic_title	RC254-282 ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma oral cancer tumor microenvironment cancer-associated fibroblast collagen tumor-infiltrating lymphocyte
topic	misc RC254-282 misc oral cancer misc tumor microenvironment misc cancer-associated fibroblast misc collagen misc tumor-infiltrating lymphocyte misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc oral cancer misc tumor microenvironment misc cancer-associated fibroblast misc collagen misc tumor-infiltrating lymphocyte misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_browse	misc RC254-282 misc oral cancer misc tumor microenvironment misc cancer-associated fibroblast misc collagen misc tumor-infiltrating lymphocyte misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma
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title_full	ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma
author_sort	Shohei Sekiguchi
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author_browse	Shohei Sekiguchi Akira Yorozu Fumika Okazaki Takeshi Niinuma Akira Takasawa Eiichiro Yamamoto Hiroshi Kitajima Toshiyuki Kubo Yui Hatanaka Koyo Nishiyama Kazuhiro Ogi Hironari Dehari Atsushi Kondo Makoto Kurose Kazufumi Obata Akito Kakiuchi Masahiro Kai Yoshihiko Hirohashi Toshihiko Torigoe Takashi Kojima Makoto Osanai Kenichi Takano Akihiro Miyazaki Hiromu Suzuki
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author-letter	Shohei Sekiguchi
doi_str_mv	10.3390/cancers15174303
author2-role	verfasserin
title_sort	aclp activates cancer-associated fibroblasts and inhibits cd8+ t-cell infiltration in oral squamous cell carcinoma
callnumber	RC254-282
title_auth	ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma
abstract	We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target.
abstractGer	We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target.
abstract_unstemmed	We previously showed that upregulation of adipocyte enhancer-binding protein 1 (<i<AEBP1</i<) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal <i<AEBP1</i</ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of <i<AEBP1</i</ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces <i<AEBP1</i</ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target.
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container_issue	17, p 4303
title_short	ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma
url	https://doi.org/10.3390/cancers15174303 https://doaj.org/article/3bd23d4ebe7c4bc39e65413a25780fe0 https://www.mdpi.com/2072-6694/15/17/4303 https://doaj.org/toc/2072-6694
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author2	Akira Yorozu Fumika Okazaki Takeshi Niinuma Akira Takasawa Eiichiro Yamamoto Hiroshi Kitajima Toshiyuki Kubo Yui Hatanaka Koyo Nishiyama Kazuhiro Ogi Hironari Dehari Atsushi Kondo Makoto Kurose Kazufumi Obata Akito Kakiuchi Masahiro Kai Yoshihiko Hirohashi Toshihiko Torigoe Takashi Kojima Makoto Osanai Kenichi Takano Akihiro Miyazaki Hiromu Suzuki
author2Str	Akira Yorozu Fumika Okazaki Takeshi Niinuma Akira Takasawa Eiichiro Yamamoto Hiroshi Kitajima Toshiyuki Kubo Yui Hatanaka Koyo Nishiyama Kazuhiro Ogi Hironari Dehari Atsushi Kondo Makoto Kurose Kazufumi Obata Akito Kakiuchi Masahiro Kai Yoshihiko Hirohashi Toshihiko Torigoe Takashi Kojima Makoto Osanai Kenichi Takano Akihiro Miyazaki Hiromu Suzuki
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up_date	2024-07-03T17:51:06.246Z
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ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma

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