Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite

A novel voltammetric sensor based on a self-assembled composite formed by native DNA and electropolymerized N-phenyl-3-(phenylimino)-3H-phenothiazin-7-amine has been developed and applied for sensitive determination of doxorubicin, an anthracycline drug applied for cancer therapy. For this purpose,...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Anastasiya Malanina [verfasserIn] Yurii Kuzin [verfasserIn] Alena Khadieva [verfasserIn] Kseniya Shibaeva [verfasserIn] Pavel Padnya [verfasserIn] Ivan Stoikov [verfasserIn] Gennady Evtugyn [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	DNA sensor electropolymerization self-assembling phenothiazine electropolymerization DNA intercalation

Übergeordnetes Werk:	In: Nanomaterials - MDPI AG, 2012, 13(2023), 16, p 2369
Übergeordnetes Werk:	volume:13 ; year:2023 ; number:16, p 2369

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/nano13162369

Katalog-ID:	DOAJ093570287

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callnumber-first	Q - Science
author	Anastasiya Malanina
spellingShingle	Anastasiya Malanina misc QD1-999 misc DNA sensor misc electropolymerization misc self-assembling misc phenothiazine electropolymerization misc DNA intercalation misc Chemistry Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite
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topic_title	QD1-999 Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite DNA sensor electropolymerization self-assembling phenothiazine electropolymerization DNA intercalation
topic	misc QD1-999 misc DNA sensor misc electropolymerization misc self-assembling misc phenothiazine electropolymerization misc DNA intercalation misc Chemistry
topic_unstemmed	misc QD1-999 misc DNA sensor misc electropolymerization misc self-assembling misc phenothiazine electropolymerization misc DNA intercalation misc Chemistry
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title	Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite
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title_full	Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite
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journal	Nanomaterials
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author_browse	Anastasiya Malanina Yurii Kuzin Alena Khadieva Kseniya Shibaeva Pavel Padnya Ivan Stoikov Gennady Evtugyn
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author-letter	Anastasiya Malanina
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title_sort	voltammetric sensor for doxorubicin determination based on self-assembled dna-polyphenothiazine composite
callnumber	QD1-999
title_auth	Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite
abstract	A novel voltammetric sensor based on a self-assembled composite formed by native DNA and electropolymerized N-phenyl-3-(phenylimino)-3H-phenothiazin-7-amine has been developed and applied for sensitive determination of doxorubicin, an anthracycline drug applied for cancer therapy. For this purpose, a monomeric phenothiazine derivative has been deposited on the glassy carbon electrode from the 0.4 M H<sub<2</sub<SO<sub<4</sub<-acetone mixture (1:1 <i<v</i</<i<v</i<) by multiple potential cycling. The DNA aliquot was either on the electrode modified with electropolymerized film or added to the reaction medium prior to electropolymerization. The DNA entrapment and its influence on the redox behavior of the underlying layer were studied by scanning electron microscopy and electrochemical impedance spectroscopy. The DNA–doxorubicin interactions affected the charge distribution in the surface layer and, hence, altered the redox equilibrium of the polyphenothiazine coating. The voltametric signal was successfully applied for the determination of doxorubicin in the concentration range from 10 pM to 0.2 mM (limit of detection 5 pM). The DNA sensor was tested on spiked artificial plasma samples and two commercial medications (recovery of 90–95%). After further testing on real clinical samples, the electrochemical DNA sensor developed can find application in monitoring drug release and screening new antitumor drugs able to intercalate DNA.
abstractGer	A novel voltammetric sensor based on a self-assembled composite formed by native DNA and electropolymerized N-phenyl-3-(phenylimino)-3H-phenothiazin-7-amine has been developed and applied for sensitive determination of doxorubicin, an anthracycline drug applied for cancer therapy. For this purpose, a monomeric phenothiazine derivative has been deposited on the glassy carbon electrode from the 0.4 M H<sub<2</sub<SO<sub<4</sub<-acetone mixture (1:1 <i<v</i</<i<v</i<) by multiple potential cycling. The DNA aliquot was either on the electrode modified with electropolymerized film or added to the reaction medium prior to electropolymerization. The DNA entrapment and its influence on the redox behavior of the underlying layer were studied by scanning electron microscopy and electrochemical impedance spectroscopy. The DNA–doxorubicin interactions affected the charge distribution in the surface layer and, hence, altered the redox equilibrium of the polyphenothiazine coating. The voltametric signal was successfully applied for the determination of doxorubicin in the concentration range from 10 pM to 0.2 mM (limit of detection 5 pM). The DNA sensor was tested on spiked artificial plasma samples and two commercial medications (recovery of 90–95%). After further testing on real clinical samples, the electrochemical DNA sensor developed can find application in monitoring drug release and screening new antitumor drugs able to intercalate DNA.
abstract_unstemmed	A novel voltammetric sensor based on a self-assembled composite formed by native DNA and electropolymerized N-phenyl-3-(phenylimino)-3H-phenothiazin-7-amine has been developed and applied for sensitive determination of doxorubicin, an anthracycline drug applied for cancer therapy. For this purpose, a monomeric phenothiazine derivative has been deposited on the glassy carbon electrode from the 0.4 M H<sub<2</sub<SO<sub<4</sub<-acetone mixture (1:1 <i<v</i</<i<v</i<) by multiple potential cycling. The DNA aliquot was either on the electrode modified with electropolymerized film or added to the reaction medium prior to electropolymerization. The DNA entrapment and its influence on the redox behavior of the underlying layer were studied by scanning electron microscopy and electrochemical impedance spectroscopy. The DNA–doxorubicin interactions affected the charge distribution in the surface layer and, hence, altered the redox equilibrium of the polyphenothiazine coating. The voltametric signal was successfully applied for the determination of doxorubicin in the concentration range from 10 pM to 0.2 mM (limit of detection 5 pM). The DNA sensor was tested on spiked artificial plasma samples and two commercial medications (recovery of 90–95%). After further testing on real clinical samples, the electrochemical DNA sensor developed can find application in monitoring drug release and screening new antitumor drugs able to intercalate DNA.
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container_issue	16, p 2369
title_short	Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite
url	https://doi.org/10.3390/nano13162369 https://doaj.org/article/cd01ef0589264f69b69ab1288389a334 https://www.mdpi.com/2079-4991/13/16/2369 https://doaj.org/toc/2079-4991
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up_date	2024-07-03T18:06:44.906Z
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Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite

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