Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain

The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence...
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Gespeichert in:

Autor*in:	Alba Martí-Marco [verfasserIn] Samantha Moratal [verfasserIn] Irene Torres-Blas [verfasserIn] Jesús Cardells [verfasserIn] Victor Lizana [verfasserIn] María Auxiliadora Dea-Ayuela [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	epidemiology molecular characterisation Spain

Übergeordnetes Werk:	In: Animals - MDPI AG, 2011, 13(2023), 15, p 2501
Übergeordnetes Werk:	volume:13 ; year:2023 ; number:15, p 2501

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ani13152501

Katalog-ID:	DOAJ093806310

Internformat


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520			\|a The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population.
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publishDate	2023
allfields	10.3390/ani13152501 doi (DE-627)DOAJ093806310 (DE-599)DOAJ3044bc399a814cbdb6a775ef1aef283d DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Alba Martí-Marco verfasserin aut Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population. <i<Cryptosporidium scrofarum</i< <i<Cryptosporidium suis</i< epidemiology <i<Giardia duodenalis</i< molecular characterisation Spain Veterinary medicine Zoology Samantha Moratal verfasserin aut Irene Torres-Blas verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut María Auxiliadora Dea-Ayuela verfasserin aut In Animals MDPI AG, 2011 13(2023), 15, p 2501 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:15, p 2501 https://doi.org/10.3390/ani13152501 kostenfrei https://doaj.org/article/3044bc399a814cbdb6a775ef1aef283d kostenfrei https://www.mdpi.com/2076-2615/13/15/2501 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 15, p 2501
spelling	10.3390/ani13152501 doi (DE-627)DOAJ093806310 (DE-599)DOAJ3044bc399a814cbdb6a775ef1aef283d DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Alba Martí-Marco verfasserin aut Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population. <i<Cryptosporidium scrofarum</i< <i<Cryptosporidium suis</i< epidemiology <i<Giardia duodenalis</i< molecular characterisation Spain Veterinary medicine Zoology Samantha Moratal verfasserin aut Irene Torres-Blas verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut María Auxiliadora Dea-Ayuela verfasserin aut In Animals MDPI AG, 2011 13(2023), 15, p 2501 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:15, p 2501 https://doi.org/10.3390/ani13152501 kostenfrei https://doaj.org/article/3044bc399a814cbdb6a775ef1aef283d kostenfrei https://www.mdpi.com/2076-2615/13/15/2501 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 15, p 2501
allfields_unstemmed	10.3390/ani13152501 doi (DE-627)DOAJ093806310 (DE-599)DOAJ3044bc399a814cbdb6a775ef1aef283d DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Alba Martí-Marco verfasserin aut Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population. <i<Cryptosporidium scrofarum</i< <i<Cryptosporidium suis</i< epidemiology <i<Giardia duodenalis</i< molecular characterisation Spain Veterinary medicine Zoology Samantha Moratal verfasserin aut Irene Torres-Blas verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut María Auxiliadora Dea-Ayuela verfasserin aut In Animals MDPI AG, 2011 13(2023), 15, p 2501 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:15, p 2501 https://doi.org/10.3390/ani13152501 kostenfrei https://doaj.org/article/3044bc399a814cbdb6a775ef1aef283d kostenfrei https://www.mdpi.com/2076-2615/13/15/2501 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 15, p 2501
allfieldsGer	10.3390/ani13152501 doi (DE-627)DOAJ093806310 (DE-599)DOAJ3044bc399a814cbdb6a775ef1aef283d DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Alba Martí-Marco verfasserin aut Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population. <i<Cryptosporidium scrofarum</i< <i<Cryptosporidium suis</i< epidemiology <i<Giardia duodenalis</i< molecular characterisation Spain Veterinary medicine Zoology Samantha Moratal verfasserin aut Irene Torres-Blas verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut María Auxiliadora Dea-Ayuela verfasserin aut In Animals MDPI AG, 2011 13(2023), 15, p 2501 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:15, p 2501 https://doi.org/10.3390/ani13152501 kostenfrei https://doaj.org/article/3044bc399a814cbdb6a775ef1aef283d kostenfrei https://www.mdpi.com/2076-2615/13/15/2501 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 15, p 2501
allfieldsSound	10.3390/ani13152501 doi (DE-627)DOAJ093806310 (DE-599)DOAJ3044bc399a814cbdb6a775ef1aef283d DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Alba Martí-Marco verfasserin aut Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population. <i<Cryptosporidium scrofarum</i< <i<Cryptosporidium suis</i< epidemiology <i<Giardia duodenalis</i< molecular characterisation Spain Veterinary medicine Zoology Samantha Moratal verfasserin aut Irene Torres-Blas verfasserin aut Jesús Cardells verfasserin aut Victor Lizana verfasserin aut María Auxiliadora Dea-Ayuela verfasserin aut In Animals MDPI AG, 2011 13(2023), 15, p 2501 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:15, p 2501 https://doi.org/10.3390/ani13152501 kostenfrei https://doaj.org/article/3044bc399a814cbdb6a775ef1aef283d kostenfrei https://www.mdpi.com/2076-2615/13/15/2501 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 15, p 2501
language	English
source	In Animals 13(2023), 15, p 2501 volume:13 year:2023 number:15, p 2501
sourceStr	In Animals 13(2023), 15, p 2501 volume:13 year:2023 number:15, p 2501
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Cryptosporidium scrofarum</i< <i<Cryptosporidium suis</i< epidemiology <i<Giardia duodenalis</i< molecular characterisation Spain Veterinary medicine Zoology
isfreeaccess_bool	true
container_title	Animals
authorswithroles_txt_mv	Alba Martí-Marco @@aut@@ Samantha Moratal @@aut@@ Irene Torres-Blas @@aut@@ Jesús Cardells @@aut@@ Victor Lizana @@aut@@ María Auxiliadora Dea-Ayuela @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	657589306
id	DOAJ093806310
language_de	englisch
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callnumber-first	S - Agriculture
author	Alba Martí-Marco
spellingShingle	Alba Martí-Marco misc SF600-1100 misc QL1-991 misc <i<Cryptosporidium scrofarum</i< misc <i<Cryptosporidium suis</i< misc epidemiology misc <i<Giardia duodenalis</i< misc molecular characterisation misc Spain misc Veterinary medicine misc Zoology Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain
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topic_title	SF600-1100 QL1-991 Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain <i<Cryptosporidium scrofarum</i< <i<Cryptosporidium suis</i< epidemiology <i<Giardia duodenalis</i< molecular characterisation Spain
topic	misc SF600-1100 misc QL1-991 misc <i<Cryptosporidium scrofarum</i< misc <i<Cryptosporidium suis</i< misc epidemiology misc <i<Giardia duodenalis</i< misc molecular characterisation misc Spain misc Veterinary medicine misc Zoology
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title	Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain
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title_full	Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain
author_sort	Alba Martí-Marco
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author_browse	Alba Martí-Marco Samantha Moratal Irene Torres-Blas Jesús Cardells Victor Lizana María Auxiliadora Dea-Ayuela
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title_sort	molecular detection and epidemiology of potentially zoonotic <i<cryptosporidium</i< spp. and <i<giardia duodenalis</i< in wild boar (<i<sus scrofa</i<) from eastern spain
callnumber	SF600-1100
title_auth	Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain
abstract	The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population.
abstractGer	The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population.
abstract_unstemmed	The protozoans <i<Giardia duodenalis</i< and <i<Cryptosporidium</i< spp. are common causes of gastrointestinal disease in humans and animals. While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. Two species of <i<Cryptosporidium</i< spp. were identified: <i<Cryptosporidium scrofarum</i< and <i<Cryptosporidium suis.</i< The results of this study demonstrate that the exposure to <i<Cryptosporidium</i< spp. in wild boars is high, particularly among young individuals belonging to the Typical Mediterranean climate. Moreover, the probability of infection is dependent on both the season and the density of wild boars. On the other side, exposure to G. duodenalis seems scarce and is influenced, in turn, by the climate. Both <i<Cryptosporidium</i< species detected in the present study have been reported in humans. Due to wild boar increasing in number and their colonisation of urban and peri-urban areas, this could represent an inherent health risk for the human population.
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title_short	Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain
url	https://doi.org/10.3390/ani13152501 https://doaj.org/article/3044bc399a814cbdb6a775ef1aef283d https://www.mdpi.com/2076-2615/13/15/2501 https://doaj.org/toc/2076-2615
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While both are commonly documented in domestic animals, few studies have analysed their presence in wildlife. To assess the prevalence of both parasites in wild boar (<i<Sus scrofa</i<) in the Valencian Community (eastern Spain), 498 wild boar faecal samples were collected from 2018 to 2022. <i<Cryptosporidium</i< spp. was detected by performing a nested PCR targeting a 578 bp sequence of the small subunit ribosomal RNA gene (SSU rRNA), followed by sequencing and phylogenetic analysis. For <i<G. duodenalis</i<, a qPCR amplifying a fragment of 62 bp from the SSU rRNA was employed. Positive samples were genotyped for glutamate dehydrogenase and β-giardin genes. Different epidemiological factors were considered potential modulating variables in the transmission of both parasites. <i<G. duodenalis</i< prevalence was 1.20%, while <i<Cryptosporidium</i< spp. prevalence reached 21.7%. Coinfection was observed in 0.2%. Genotyping of <i<G. duodenalis</i< isolates only detected genotype E. 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Molecular Detection and Epidemiology of Potentially Zoonotic <i<Cryptosporidium</i< spp. and <i<Giardia duodenalis</i< in Wild Boar (<i<Sus scrofa</i<) from Eastern Spain

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