qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer

Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Zafar Iqbal [verfasserIn] Muhammad Shafiq [verfasserIn] Sajed Ali [verfasserIn] Muhammad Arslan Mahmood [verfasserIn] Hamid Anees Siddiqui [verfasserIn] Imran Amin [verfasserIn] Rob W. Briddon [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	national coordinated varietal trials (NCVT) seed cotton yield (SCY) cotton leaf curl disease (CLCuD) cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) cotton leaf curl Multan betasatellite (CLCuMuB) quantitative real-time PCR (qPCR)

Übergeordnetes Werk:	In: Plants - MDPI AG, 2013, 12(2023), 14, p 2645
Übergeordnetes Werk:	volume:12 ; year:2023 ; number:14, p 2645

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/plants12142645

Katalog-ID:	DOAJ093838905

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allfields	10.3390/plants12142645 doi (DE-627)DOAJ093838905 (DE-599)DOAJ25000bfa2f21418da35a3195f32c54a3 DE-627 ger DE-627 rakwb eng QK1-989 Zafar Iqbal verfasserin aut qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan. national coordinated varietal trials (NCVT) seed cotton yield (SCY) cotton leaf curl disease (CLCuD) cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) cotton leaf curl Multan betasatellite (CLCuMuB) quantitative real-time PCR (qPCR) Botany Muhammad Shafiq verfasserin aut Sajed Ali verfasserin aut Muhammad Arslan Mahmood verfasserin aut Hamid Anees Siddiqui verfasserin aut Imran Amin verfasserin aut Rob W. Briddon verfasserin aut In Plants MDPI AG, 2013 12(2023), 14, p 2645 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:14, p 2645 https://doi.org/10.3390/plants12142645 kostenfrei https://doaj.org/article/25000bfa2f21418da35a3195f32c54a3 kostenfrei https://www.mdpi.com/2223-7747/12/14/2645 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 14, p 2645
spelling	10.3390/plants12142645 doi (DE-627)DOAJ093838905 (DE-599)DOAJ25000bfa2f21418da35a3195f32c54a3 DE-627 ger DE-627 rakwb eng QK1-989 Zafar Iqbal verfasserin aut qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan. national coordinated varietal trials (NCVT) seed cotton yield (SCY) cotton leaf curl disease (CLCuD) cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) cotton leaf curl Multan betasatellite (CLCuMuB) quantitative real-time PCR (qPCR) Botany Muhammad Shafiq verfasserin aut Sajed Ali verfasserin aut Muhammad Arslan Mahmood verfasserin aut Hamid Anees Siddiqui verfasserin aut Imran Amin verfasserin aut Rob W. Briddon verfasserin aut In Plants MDPI AG, 2013 12(2023), 14, p 2645 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:14, p 2645 https://doi.org/10.3390/plants12142645 kostenfrei https://doaj.org/article/25000bfa2f21418da35a3195f32c54a3 kostenfrei https://www.mdpi.com/2223-7747/12/14/2645 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 14, p 2645
allfields_unstemmed	10.3390/plants12142645 doi (DE-627)DOAJ093838905 (DE-599)DOAJ25000bfa2f21418da35a3195f32c54a3 DE-627 ger DE-627 rakwb eng QK1-989 Zafar Iqbal verfasserin aut qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan. national coordinated varietal trials (NCVT) seed cotton yield (SCY) cotton leaf curl disease (CLCuD) cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) cotton leaf curl Multan betasatellite (CLCuMuB) quantitative real-time PCR (qPCR) Botany Muhammad Shafiq verfasserin aut Sajed Ali verfasserin aut Muhammad Arslan Mahmood verfasserin aut Hamid Anees Siddiqui verfasserin aut Imran Amin verfasserin aut Rob W. Briddon verfasserin aut In Plants MDPI AG, 2013 12(2023), 14, p 2645 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:14, p 2645 https://doi.org/10.3390/plants12142645 kostenfrei https://doaj.org/article/25000bfa2f21418da35a3195f32c54a3 kostenfrei https://www.mdpi.com/2223-7747/12/14/2645 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 14, p 2645
allfieldsGer	10.3390/plants12142645 doi (DE-627)DOAJ093838905 (DE-599)DOAJ25000bfa2f21418da35a3195f32c54a3 DE-627 ger DE-627 rakwb eng QK1-989 Zafar Iqbal verfasserin aut qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan. national coordinated varietal trials (NCVT) seed cotton yield (SCY) cotton leaf curl disease (CLCuD) cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) cotton leaf curl Multan betasatellite (CLCuMuB) quantitative real-time PCR (qPCR) Botany Muhammad Shafiq verfasserin aut Sajed Ali verfasserin aut Muhammad Arslan Mahmood verfasserin aut Hamid Anees Siddiqui verfasserin aut Imran Amin verfasserin aut Rob W. Briddon verfasserin aut In Plants MDPI AG, 2013 12(2023), 14, p 2645 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:14, p 2645 https://doi.org/10.3390/plants12142645 kostenfrei https://doaj.org/article/25000bfa2f21418da35a3195f32c54a3 kostenfrei https://www.mdpi.com/2223-7747/12/14/2645 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 14, p 2645
allfieldsSound	10.3390/plants12142645 doi (DE-627)DOAJ093838905 (DE-599)DOAJ25000bfa2f21418da35a3195f32c54a3 DE-627 ger DE-627 rakwb eng QK1-989 Zafar Iqbal verfasserin aut qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan. national coordinated varietal trials (NCVT) seed cotton yield (SCY) cotton leaf curl disease (CLCuD) cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) cotton leaf curl Multan betasatellite (CLCuMuB) quantitative real-time PCR (qPCR) Botany Muhammad Shafiq verfasserin aut Sajed Ali verfasserin aut Muhammad Arslan Mahmood verfasserin aut Hamid Anees Siddiqui verfasserin aut Imran Amin verfasserin aut Rob W. Briddon verfasserin aut In Plants MDPI AG, 2013 12(2023), 14, p 2645 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:14, p 2645 https://doi.org/10.3390/plants12142645 kostenfrei https://doaj.org/article/25000bfa2f21418da35a3195f32c54a3 kostenfrei https://www.mdpi.com/2223-7747/12/14/2645 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 14, p 2645
language	English
source	In Plants 12(2023), 14, p 2645 volume:12 year:2023 number:14, p 2645
sourceStr	In Plants 12(2023), 14, p 2645 volume:12 year:2023 number:14, p 2645
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	national coordinated varietal trials (NCVT) seed cotton yield (SCY) cotton leaf curl disease (CLCuD) cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) cotton leaf curl Multan betasatellite (CLCuMuB) quantitative real-time PCR (qPCR) Botany
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container_title	Plants
authorswithroles_txt_mv	Zafar Iqbal @@aut@@ Muhammad Shafiq @@aut@@ Sajed Ali @@aut@@ Muhammad Arslan Mahmood @@aut@@ Hamid Anees Siddiqui @@aut@@ Imran Amin @@aut@@ Rob W. Briddon @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	737288345
id	DOAJ093838905
language_de	englisch
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callnumber-first	Q - Science
author	Zafar Iqbal
spellingShingle	Zafar Iqbal misc QK1-989 misc national coordinated varietal trials (NCVT) misc seed cotton yield (SCY) misc cotton leaf curl disease (CLCuD) misc cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) misc cotton leaf curl Multan betasatellite (CLCuMuB) misc quantitative real-time PCR (qPCR) misc Botany qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer
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topic_title	QK1-989 qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer national coordinated varietal trials (NCVT) seed cotton yield (SCY) cotton leaf curl disease (CLCuD) cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) cotton leaf curl Multan betasatellite (CLCuMuB) quantitative real-time PCR (qPCR)
topic	misc QK1-989 misc national coordinated varietal trials (NCVT) misc seed cotton yield (SCY) misc cotton leaf curl disease (CLCuD) misc cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) misc cotton leaf curl Multan betasatellite (CLCuMuB) misc quantitative real-time PCR (qPCR) misc Botany
topic_unstemmed	misc QK1-989 misc national coordinated varietal trials (NCVT) misc seed cotton yield (SCY) misc cotton leaf curl disease (CLCuD) misc cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) misc cotton leaf curl Multan betasatellite (CLCuMuB) misc quantitative real-time PCR (qPCR) misc Botany
topic_browse	misc QK1-989 misc national coordinated varietal trials (NCVT) misc seed cotton yield (SCY) misc cotton leaf curl disease (CLCuD) misc cotton leaf curl Khokhran virus—Burewala strain (CLCuKoV-Bur) misc cotton leaf curl Multan betasatellite (CLCuMuB) misc quantitative real-time PCR (qPCR) misc Botany
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title	qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer
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title_full	qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer
author_sort	Zafar Iqbal
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author_browse	Zafar Iqbal Muhammad Shafiq Sajed Ali Muhammad Arslan Mahmood Hamid Anees Siddiqui Imran Amin Rob W. Briddon
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title_sort	qpcr assay as a tool for examining cotton resistance to the virus complex causing clcud: yield loss inversely correlates with betasatellite, not virus, dna titer
callnumber	QK1-989
title_auth	qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer
abstract	Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan.
abstractGer	Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan.
abstract_unstemmed	Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus <i<Begomovirus</i<, family <i<Geminiviridae</i<) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-3 (0.006 ng·µg<sup<−1</sup<) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg<sup<−1</sup<) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg<sup<−1</sup<) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg<sup<−1</sup<) from Faisalabad and the lowest in B-12 (0.072 ng·µg<sup<−1</sup<) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan.
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title_short	qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer
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qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer

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