Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis

Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel...
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Autor*in:	Poornima K. Gopalakrishna [verfasserIn] Rajamma Abburu Jayaramu [verfasserIn] Sateesha Shivally Boregowda [verfasserIn] Shruthi Eshwar [verfasserIn] Nikhil V. Suresh [verfasserIn] Amr Selim Abu Lila [verfasserIn] Afrasim Moin [verfasserIn] Hadil Faris Alotaibi [verfasserIn] Ahmad J. Obaidullah [verfasserIn] El-Sayed Khafagy [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	anti-inflammatory anti-plaque in situ gel periodontitis piperine

Übergeordnetes Werk:	In: Gels - MDPI AG, 2015, 9(2023), 7, p 577
Übergeordnetes Werk:	volume:9 ; year:2023 ; number:7, p 577

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/gels9070577

Katalog-ID:	DOAJ093899556

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520			\|a Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases.
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allfields	10.3390/gels9070577 doi (DE-627)DOAJ093899556 (DE-599)DOAJc45ed8def70640ed84d0eb3d80df77c0 DE-627 ger DE-627 rakwb eng QD1-999 QD146-197 QD1-65 Poornima K. Gopalakrishna verfasserin aut Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases. anti-inflammatory anti-plaque in situ gel periodontitis piperine Science Q Chemistry Inorganic chemistry General. Including alchemy Rajamma Abburu Jayaramu verfasserin aut Sateesha Shivally Boregowda verfasserin aut Shruthi Eshwar verfasserin aut Nikhil V. Suresh verfasserin aut Amr Selim Abu Lila verfasserin aut Afrasim Moin verfasserin aut Hadil Faris Alotaibi verfasserin aut Ahmad J. Obaidullah verfasserin aut El-Sayed Khafagy verfasserin aut In Gels MDPI AG, 2015 9(2023), 7, p 577 (DE-627)820684147 (DE-600)2813982-3 23102861 nnns volume:9 year:2023 number:7, p 577 https://doi.org/10.3390/gels9070577 kostenfrei https://doaj.org/article/c45ed8def70640ed84d0eb3d80df77c0 kostenfrei https://www.mdpi.com/2310-2861/9/7/577 kostenfrei https://doaj.org/toc/2310-2861 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 7, p 577
spelling	10.3390/gels9070577 doi (DE-627)DOAJ093899556 (DE-599)DOAJc45ed8def70640ed84d0eb3d80df77c0 DE-627 ger DE-627 rakwb eng QD1-999 QD146-197 QD1-65 Poornima K. Gopalakrishna verfasserin aut Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases. anti-inflammatory anti-plaque in situ gel periodontitis piperine Science Q Chemistry Inorganic chemistry General. Including alchemy Rajamma Abburu Jayaramu verfasserin aut Sateesha Shivally Boregowda verfasserin aut Shruthi Eshwar verfasserin aut Nikhil V. Suresh verfasserin aut Amr Selim Abu Lila verfasserin aut Afrasim Moin verfasserin aut Hadil Faris Alotaibi verfasserin aut Ahmad J. Obaidullah verfasserin aut El-Sayed Khafagy verfasserin aut In Gels MDPI AG, 2015 9(2023), 7, p 577 (DE-627)820684147 (DE-600)2813982-3 23102861 nnns volume:9 year:2023 number:7, p 577 https://doi.org/10.3390/gels9070577 kostenfrei https://doaj.org/article/c45ed8def70640ed84d0eb3d80df77c0 kostenfrei https://www.mdpi.com/2310-2861/9/7/577 kostenfrei https://doaj.org/toc/2310-2861 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 7, p 577
allfields_unstemmed	10.3390/gels9070577 doi (DE-627)DOAJ093899556 (DE-599)DOAJc45ed8def70640ed84d0eb3d80df77c0 DE-627 ger DE-627 rakwb eng QD1-999 QD146-197 QD1-65 Poornima K. Gopalakrishna verfasserin aut Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases. anti-inflammatory anti-plaque in situ gel periodontitis piperine Science Q Chemistry Inorganic chemistry General. Including alchemy Rajamma Abburu Jayaramu verfasserin aut Sateesha Shivally Boregowda verfasserin aut Shruthi Eshwar verfasserin aut Nikhil V. Suresh verfasserin aut Amr Selim Abu Lila verfasserin aut Afrasim Moin verfasserin aut Hadil Faris Alotaibi verfasserin aut Ahmad J. Obaidullah verfasserin aut El-Sayed Khafagy verfasserin aut In Gels MDPI AG, 2015 9(2023), 7, p 577 (DE-627)820684147 (DE-600)2813982-3 23102861 nnns volume:9 year:2023 number:7, p 577 https://doi.org/10.3390/gels9070577 kostenfrei https://doaj.org/article/c45ed8def70640ed84d0eb3d80df77c0 kostenfrei https://www.mdpi.com/2310-2861/9/7/577 kostenfrei https://doaj.org/toc/2310-2861 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 7, p 577
allfieldsGer	10.3390/gels9070577 doi (DE-627)DOAJ093899556 (DE-599)DOAJc45ed8def70640ed84d0eb3d80df77c0 DE-627 ger DE-627 rakwb eng QD1-999 QD146-197 QD1-65 Poornima K. Gopalakrishna verfasserin aut Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases. anti-inflammatory anti-plaque in situ gel periodontitis piperine Science Q Chemistry Inorganic chemistry General. Including alchemy Rajamma Abburu Jayaramu verfasserin aut Sateesha Shivally Boregowda verfasserin aut Shruthi Eshwar verfasserin aut Nikhil V. Suresh verfasserin aut Amr Selim Abu Lila verfasserin aut Afrasim Moin verfasserin aut Hadil Faris Alotaibi verfasserin aut Ahmad J. Obaidullah verfasserin aut El-Sayed Khafagy verfasserin aut In Gels MDPI AG, 2015 9(2023), 7, p 577 (DE-627)820684147 (DE-600)2813982-3 23102861 nnns volume:9 year:2023 number:7, p 577 https://doi.org/10.3390/gels9070577 kostenfrei https://doaj.org/article/c45ed8def70640ed84d0eb3d80df77c0 kostenfrei https://www.mdpi.com/2310-2861/9/7/577 kostenfrei https://doaj.org/toc/2310-2861 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 7, p 577
allfieldsSound	10.3390/gels9070577 doi (DE-627)DOAJ093899556 (DE-599)DOAJc45ed8def70640ed84d0eb3d80df77c0 DE-627 ger DE-627 rakwb eng QD1-999 QD146-197 QD1-65 Poornima K. Gopalakrishna verfasserin aut Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases. anti-inflammatory anti-plaque in situ gel periodontitis piperine Science Q Chemistry Inorganic chemistry General. Including alchemy Rajamma Abburu Jayaramu verfasserin aut Sateesha Shivally Boregowda verfasserin aut Shruthi Eshwar verfasserin aut Nikhil V. Suresh verfasserin aut Amr Selim Abu Lila verfasserin aut Afrasim Moin verfasserin aut Hadil Faris Alotaibi verfasserin aut Ahmad J. Obaidullah verfasserin aut El-Sayed Khafagy verfasserin aut In Gels MDPI AG, 2015 9(2023), 7, p 577 (DE-627)820684147 (DE-600)2813982-3 23102861 nnns volume:9 year:2023 number:7, p 577 https://doi.org/10.3390/gels9070577 kostenfrei https://doaj.org/article/c45ed8def70640ed84d0eb3d80df77c0 kostenfrei https://www.mdpi.com/2310-2861/9/7/577 kostenfrei https://doaj.org/toc/2310-2861 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 7, p 577
language	English
source	In Gels 9(2023), 7, p 577 volume:9 year:2023 number:7, p 577
sourceStr	In Gels 9(2023), 7, p 577 volume:9 year:2023 number:7, p 577
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	anti-inflammatory anti-plaque in situ gel periodontitis piperine Science Q Chemistry Inorganic chemistry General. Including alchemy
isfreeaccess_bool	true
container_title	Gels
authorswithroles_txt_mv	Poornima K. Gopalakrishna @@aut@@ Rajamma Abburu Jayaramu @@aut@@ Sateesha Shivally Boregowda @@aut@@ Shruthi Eshwar @@aut@@ Nikhil V. Suresh @@aut@@ Amr Selim Abu Lila @@aut@@ Afrasim Moin @@aut@@ Hadil Faris Alotaibi @@aut@@ Ahmad J. Obaidullah @@aut@@ El-Sayed Khafagy @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	820684147
id	DOAJ093899556
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callnumber-first	Q - Science
author	Poornima K. Gopalakrishna
spellingShingle	Poornima K. Gopalakrishna misc QD1-999 misc QD146-197 misc QD1-65 misc anti-inflammatory misc anti-plaque misc in situ gel misc periodontitis misc piperine misc Science misc Q misc Chemistry misc Inorganic chemistry misc General. Including alchemy Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis
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topic_title	QD1-999 QD146-197 QD1-65 Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis anti-inflammatory anti-plaque in situ gel periodontitis piperine
topic	misc QD1-999 misc QD146-197 misc QD1-65 misc anti-inflammatory misc anti-plaque misc in situ gel misc periodontitis misc piperine misc Science misc Q misc Chemistry misc Inorganic chemistry misc General. Including alchemy
topic_unstemmed	misc QD1-999 misc QD146-197 misc QD1-65 misc anti-inflammatory misc anti-plaque misc in situ gel misc periodontitis misc piperine misc Science misc Q misc Chemistry misc Inorganic chemistry misc General. Including alchemy
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title	Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis
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title_full	Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis
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author_browse	Poornima K. Gopalakrishna Rajamma Abburu Jayaramu Sateesha Shivally Boregowda Shruthi Eshwar Nikhil V. Suresh Amr Selim Abu Lila Afrasim Moin Hadil Faris Alotaibi Ahmad J. Obaidullah El-Sayed Khafagy
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title_sort	piperine-loaded in situ gel: formulation, in vitro characterization, and clinical evaluation against periodontitis
callnumber	QD1-999
title_auth	Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis
abstract	Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases.
abstractGer	Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases.
abstract_unstemmed	Periodontitis is an inflammatory disorder associated with dysbiosis and characterized by microbiologically related, host-mediated inflammation that leads to the damage of periodontal tissues including gingiva, connective tissues, and alveolar bone. The aim of this study was to develop an in situ gel consisting of piperine. Eight in situ gel formulations were designed by varying the concentration of deacylated gellan gum cross-linked with sodium tripolyphosphate, and poloxamer-407. The prepared gels were evaluated for gelation temperature, gelation time, viscosity, piperine-loading efficiency, and piperine release. Finally, the optimized formula was evaluated for anti-inflammatory effectiveness among human patients during a 14-day follow-up. The optimized in situ gel formulation exhibited a gelation temperature of 35 ± 1 °C, gelling of 36 ± 1 s, excellent syringeability, and piperine loading of 95.3 ± 2.3%. This formulation efficiently sustained in vitro drug release for up to 72 h. In vivo studies revealed an efficient sol-to-gel transformation of optimized in situ gel formulation at physiological conditions, permitting an efficient residence time of the formulation within a periodontitis pocket. Most importantly, a clinical study revealed that treatment with the optimized formulation elicited a significant reduction in the mean plaque score (<i<p</i< = 0.001), gingival index (<i<p</i< = 0.003), and pocket depth (<i<p</i< = 0.002), and exerted a potent anti-inflammatory potential, compared to the control group. Collectively, piperine-loaded in situ gel might represent a viable therapeutic approach for the management of gingival and periodontal diseases.
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title_short	Piperine-Loaded In Situ Gel: Formulation, In Vitro Characterization, and Clinical Evaluation against Periodontitis
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author2	Rajamma Abburu Jayaramu Sateesha Shivally Boregowda Shruthi Eshwar Nikhil V. Suresh Amr Selim Abu Lila Afrasim Moin Hadil Faris Alotaibi Ahmad J. Obaidullah El-Sayed Khafagy
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up_date	2024-07-03T20:03:41.266Z
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