Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX...
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Gespeichert in:

Autor*in:	Zhongjing Zhou [verfasserIn] Zhiping Deng [verfasserIn] Shuang Liang [verfasserIn] Xiaowei Zou [verfasserIn] Yi Teng [verfasserIn] Weike Wang [verfasserIn] Lizhong Fu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	flavonoids UHPLC-MS/MS quantitative

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 28(2023), 13, p 5166
Übergeordnetes Werk:	volume:28 ; year:2023 ; number:13, p 5166

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules28135166

Katalog-ID:	DOAJ093992602

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allfields	10.3390/molecules28135166 doi (DE-627)DOAJ093992602 (DE-599)DOAJ249cb487ecee47688098aa3faaf3ac17 DE-627 ger DE-627 rakwb eng QD241-441 Zhongjing Zhou verfasserin aut Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different <i<Sanghuangporus</i< species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds. flavonoids UHPLC-MS/MS <i<Sanghuangporus</i< quantitative Organic chemistry Zhiping Deng verfasserin aut Shuang Liang verfasserin aut Xiaowei Zou verfasserin aut Yi Teng verfasserin aut Weike Wang verfasserin aut Lizhong Fu verfasserin aut In Molecules MDPI AG, 2003 28(2023), 13, p 5166 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:13, p 5166 https://doi.org/10.3390/molecules28135166 kostenfrei https://doaj.org/article/249cb487ecee47688098aa3faaf3ac17 kostenfrei https://www.mdpi.com/1420-3049/28/13/5166 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 13, p 5166
spelling	10.3390/molecules28135166 doi (DE-627)DOAJ093992602 (DE-599)DOAJ249cb487ecee47688098aa3faaf3ac17 DE-627 ger DE-627 rakwb eng QD241-441 Zhongjing Zhou verfasserin aut Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different <i<Sanghuangporus</i< species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds. flavonoids UHPLC-MS/MS <i<Sanghuangporus</i< quantitative Organic chemistry Zhiping Deng verfasserin aut Shuang Liang verfasserin aut Xiaowei Zou verfasserin aut Yi Teng verfasserin aut Weike Wang verfasserin aut Lizhong Fu verfasserin aut In Molecules MDPI AG, 2003 28(2023), 13, p 5166 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:13, p 5166 https://doi.org/10.3390/molecules28135166 kostenfrei https://doaj.org/article/249cb487ecee47688098aa3faaf3ac17 kostenfrei https://www.mdpi.com/1420-3049/28/13/5166 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 13, p 5166
allfields_unstemmed	10.3390/molecules28135166 doi (DE-627)DOAJ093992602 (DE-599)DOAJ249cb487ecee47688098aa3faaf3ac17 DE-627 ger DE-627 rakwb eng QD241-441 Zhongjing Zhou verfasserin aut Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different <i<Sanghuangporus</i< species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds. flavonoids UHPLC-MS/MS <i<Sanghuangporus</i< quantitative Organic chemistry Zhiping Deng verfasserin aut Shuang Liang verfasserin aut Xiaowei Zou verfasserin aut Yi Teng verfasserin aut Weike Wang verfasserin aut Lizhong Fu verfasserin aut In Molecules MDPI AG, 2003 28(2023), 13, p 5166 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:13, p 5166 https://doi.org/10.3390/molecules28135166 kostenfrei https://doaj.org/article/249cb487ecee47688098aa3faaf3ac17 kostenfrei https://www.mdpi.com/1420-3049/28/13/5166 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 13, p 5166
allfieldsGer	10.3390/molecules28135166 doi (DE-627)DOAJ093992602 (DE-599)DOAJ249cb487ecee47688098aa3faaf3ac17 DE-627 ger DE-627 rakwb eng QD241-441 Zhongjing Zhou verfasserin aut Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different <i<Sanghuangporus</i< species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds. flavonoids UHPLC-MS/MS <i<Sanghuangporus</i< quantitative Organic chemistry Zhiping Deng verfasserin aut Shuang Liang verfasserin aut Xiaowei Zou verfasserin aut Yi Teng verfasserin aut Weike Wang verfasserin aut Lizhong Fu verfasserin aut In Molecules MDPI AG, 2003 28(2023), 13, p 5166 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:13, p 5166 https://doi.org/10.3390/molecules28135166 kostenfrei https://doaj.org/article/249cb487ecee47688098aa3faaf3ac17 kostenfrei https://www.mdpi.com/1420-3049/28/13/5166 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 13, p 5166
allfieldsSound	10.3390/molecules28135166 doi (DE-627)DOAJ093992602 (DE-599)DOAJ249cb487ecee47688098aa3faaf3ac17 DE-627 ger DE-627 rakwb eng QD241-441 Zhongjing Zhou verfasserin aut Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different <i<Sanghuangporus</i< species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds. flavonoids UHPLC-MS/MS <i<Sanghuangporus</i< quantitative Organic chemistry Zhiping Deng verfasserin aut Shuang Liang verfasserin aut Xiaowei Zou verfasserin aut Yi Teng verfasserin aut Weike Wang verfasserin aut Lizhong Fu verfasserin aut In Molecules MDPI AG, 2003 28(2023), 13, p 5166 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:13, p 5166 https://doi.org/10.3390/molecules28135166 kostenfrei https://doaj.org/article/249cb487ecee47688098aa3faaf3ac17 kostenfrei https://www.mdpi.com/1420-3049/28/13/5166 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 13, p 5166
language	English
source	In Molecules 28(2023), 13, p 5166 volume:28 year:2023 number:13, p 5166
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title_sort	quantitative analysis of flavonoids in fruiting bodies of <i<sanghuangporus</i< using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry
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title_auth	Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry
abstract	A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different <i<Sanghuangporus</i< species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds.
abstractGer	A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different <i<Sanghuangporus</i< species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds.
abstract_unstemmed	A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of <i<Sanghuangporus</i< was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different <i<Sanghuangporus</i< species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds.
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title_short	Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry
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Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-<i<O</i<-rutinoside, catechin, and catechin gallate) in the fruiting bodies of <i<Sanghuangporus</i<. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various <i<Sanghuangporus</i< species. 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Quantitative Analysis of Flavonoids in Fruiting Bodies of <i<Sanghuangporus</i< Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

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