Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification

Cellulase has been widely used in many industrial fields, such as feed and food industry, because it can hydrolyze cellulose to oligosaccharides with a lower degree of polymerization. Endo-β-1,4-glucanase is a critical speed-limiting cellulase in the saccharification process. In this study, endo-β-1...
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Autor*in:	Zhen Huang [verfasserIn] Guorong Ni [verfasserIn] Longhua Dai [verfasserIn] Weiqi Zhang [verfasserIn] Siting Feng [verfasserIn] Fei Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	endo-β-1,4-glucanase expression characterization oligosaccharide saccharification

Übergeordnetes Werk:	In: Foods - MDPI AG, 2013, 12(2023), 13, p 2517
Übergeordnetes Werk:	volume:12 ; year:2023 ; number:13, p 2517

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/foods12132517

Katalog-ID:	DOAJ094016755

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allfields	10.3390/foods12132517 doi (DE-627)DOAJ094016755 (DE-599)DOAJ646e2204e87d412181da2dedcb15170c DE-627 ger DE-627 rakwb eng TP1-1185 Zhen Huang verfasserin aut Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cellulase has been widely used in many industrial fields, such as feed and food industry, because it can hydrolyze cellulose to oligosaccharides with a lower degree of polymerization. Endo-β-1,4-glucanase is a critical speed-limiting cellulase in the saccharification process. In this study, endo-β-1,4-glucanase gene (<i<CelA257</i<) from <i<Myxococcus</i< sp. B6-1 was cloned and expressed in <i<Escherichia coli</i<. CelA257 contained carbohydrate-binding module (CBM) 4-9 and glycosyl hydrolase (GH) family 6 domain that shares 54.7% identity with endoglucanase from <i<Streptomyces halstedii</i<. The recombinant enzyme exhibited optimal activity at pH 6.5 and 50 °C and was stable over a broad pH (6–9.5) range and temperature < 50 °C. CelA257 exhibited broad substrate specificity to barley β-glucan, lichenin, CMC, chitosan, laminarin, avicel, and phosphoric acid swollen cellulose (PASC). CelA257 degraded both cellotetrose (G<sub<4</sub<) and cellppentaose (G<sub<5</sub<) to cellobiose (G<sub<2</sub<) and cellotriose (G<sub<3</sub<). Adding CelA257 increased the release of reducing sugars in crop straw powers, including wheat straw (0.18 mg/mL), rape straw (0.42 mg/mL), rice straw (0.16 mg/mL), peanut straw (0.16 mg/mL), and corn straw (0.61 mg/mL). This study provides a potential additive in biomass saccharification applications. endo-β-1,4-glucanase <i<Escherichia coli</i< expression characterization oligosaccharide saccharification Chemical technology Guorong Ni verfasserin aut Longhua Dai verfasserin aut Weiqi Zhang verfasserin aut Siting Feng verfasserin aut Fei Wang verfasserin aut In Foods MDPI AG, 2013 12(2023), 13, p 2517 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:12 year:2023 number:13, p 2517 https://doi.org/10.3390/foods12132517 kostenfrei https://doaj.org/article/646e2204e87d412181da2dedcb15170c kostenfrei https://www.mdpi.com/2304-8158/12/13/2517 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 13, p 2517
spelling	10.3390/foods12132517 doi (DE-627)DOAJ094016755 (DE-599)DOAJ646e2204e87d412181da2dedcb15170c DE-627 ger DE-627 rakwb eng TP1-1185 Zhen Huang verfasserin aut Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cellulase has been widely used in many industrial fields, such as feed and food industry, because it can hydrolyze cellulose to oligosaccharides with a lower degree of polymerization. Endo-β-1,4-glucanase is a critical speed-limiting cellulase in the saccharification process. In this study, endo-β-1,4-glucanase gene (<i<CelA257</i<) from <i<Myxococcus</i< sp. B6-1 was cloned and expressed in <i<Escherichia coli</i<. CelA257 contained carbohydrate-binding module (CBM) 4-9 and glycosyl hydrolase (GH) family 6 domain that shares 54.7% identity with endoglucanase from <i<Streptomyces halstedii</i<. The recombinant enzyme exhibited optimal activity at pH 6.5 and 50 °C and was stable over a broad pH (6–9.5) range and temperature < 50 °C. CelA257 exhibited broad substrate specificity to barley β-glucan, lichenin, CMC, chitosan, laminarin, avicel, and phosphoric acid swollen cellulose (PASC). CelA257 degraded both cellotetrose (G<sub<4</sub<) and cellppentaose (G<sub<5</sub<) to cellobiose (G<sub<2</sub<) and cellotriose (G<sub<3</sub<). Adding CelA257 increased the release of reducing sugars in crop straw powers, including wheat straw (0.18 mg/mL), rape straw (0.42 mg/mL), rice straw (0.16 mg/mL), peanut straw (0.16 mg/mL), and corn straw (0.61 mg/mL). This study provides a potential additive in biomass saccharification applications. endo-β-1,4-glucanase <i<Escherichia coli</i< expression characterization oligosaccharide saccharification Chemical technology Guorong Ni verfasserin aut Longhua Dai verfasserin aut Weiqi Zhang verfasserin aut Siting Feng verfasserin aut Fei Wang verfasserin aut In Foods MDPI AG, 2013 12(2023), 13, p 2517 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:12 year:2023 number:13, p 2517 https://doi.org/10.3390/foods12132517 kostenfrei https://doaj.org/article/646e2204e87d412181da2dedcb15170c kostenfrei https://www.mdpi.com/2304-8158/12/13/2517 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 13, p 2517
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allfieldsSound	10.3390/foods12132517 doi (DE-627)DOAJ094016755 (DE-599)DOAJ646e2204e87d412181da2dedcb15170c DE-627 ger DE-627 rakwb eng TP1-1185 Zhen Huang verfasserin aut Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cellulase has been widely used in many industrial fields, such as feed and food industry, because it can hydrolyze cellulose to oligosaccharides with a lower degree of polymerization. Endo-β-1,4-glucanase is a critical speed-limiting cellulase in the saccharification process. In this study, endo-β-1,4-glucanase gene (<i<CelA257</i<) from <i<Myxococcus</i< sp. B6-1 was cloned and expressed in <i<Escherichia coli</i<. CelA257 contained carbohydrate-binding module (CBM) 4-9 and glycosyl hydrolase (GH) family 6 domain that shares 54.7% identity with endoglucanase from <i<Streptomyces halstedii</i<. The recombinant enzyme exhibited optimal activity at pH 6.5 and 50 °C and was stable over a broad pH (6–9.5) range and temperature < 50 °C. CelA257 exhibited broad substrate specificity to barley β-glucan, lichenin, CMC, chitosan, laminarin, avicel, and phosphoric acid swollen cellulose (PASC). CelA257 degraded both cellotetrose (G<sub<4</sub<) and cellppentaose (G<sub<5</sub<) to cellobiose (G<sub<2</sub<) and cellotriose (G<sub<3</sub<). Adding CelA257 increased the release of reducing sugars in crop straw powers, including wheat straw (0.18 mg/mL), rape straw (0.42 mg/mL), rice straw (0.16 mg/mL), peanut straw (0.16 mg/mL), and corn straw (0.61 mg/mL). This study provides a potential additive in biomass saccharification applications. endo-β-1,4-glucanase <i<Escherichia coli</i< expression characterization oligosaccharide saccharification Chemical technology Guorong Ni verfasserin aut Longhua Dai verfasserin aut Weiqi Zhang verfasserin aut Siting Feng verfasserin aut Fei Wang verfasserin aut In Foods MDPI AG, 2013 12(2023), 13, p 2517 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:12 year:2023 number:13, p 2517 https://doi.org/10.3390/foods12132517 kostenfrei https://doaj.org/article/646e2204e87d412181da2dedcb15170c kostenfrei https://www.mdpi.com/2304-8158/12/13/2517 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 13, p 2517
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callnumber-first	T - Technology
author	Zhen Huang
spellingShingle	Zhen Huang misc TP1-1185 misc endo-β-1,4-glucanase misc <i<Escherichia coli</i< misc expression misc characterization misc oligosaccharide misc saccharification misc Chemical technology Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification
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topic_title	TP1-1185 Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification endo-β-1,4-glucanase <i<Escherichia coli</i< expression characterization oligosaccharide saccharification
topic	misc TP1-1185 misc endo-β-1,4-glucanase misc <i<Escherichia coli</i< misc expression misc characterization misc oligosaccharide misc saccharification misc Chemical technology
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title	Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification
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title_full	Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification
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title_sort	biochemical characterization of novel gh6 endoglucanase from <i<myxococcus</i< sp. b6-1 and its effects on agricultural straws saccharification
callnumber	TP1-1185
title_auth	Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification
abstract	Cellulase has been widely used in many industrial fields, such as feed and food industry, because it can hydrolyze cellulose to oligosaccharides with a lower degree of polymerization. Endo-β-1,4-glucanase is a critical speed-limiting cellulase in the saccharification process. In this study, endo-β-1,4-glucanase gene (<i<CelA257</i<) from <i<Myxococcus</i< sp. B6-1 was cloned and expressed in <i<Escherichia coli</i<. CelA257 contained carbohydrate-binding module (CBM) 4-9 and glycosyl hydrolase (GH) family 6 domain that shares 54.7% identity with endoglucanase from <i<Streptomyces halstedii</i<. The recombinant enzyme exhibited optimal activity at pH 6.5 and 50 °C and was stable over a broad pH (6–9.5) range and temperature < 50 °C. CelA257 exhibited broad substrate specificity to barley β-glucan, lichenin, CMC, chitosan, laminarin, avicel, and phosphoric acid swollen cellulose (PASC). CelA257 degraded both cellotetrose (G<sub<4</sub<) and cellppentaose (G<sub<5</sub<) to cellobiose (G<sub<2</sub<) and cellotriose (G<sub<3</sub<). Adding CelA257 increased the release of reducing sugars in crop straw powers, including wheat straw (0.18 mg/mL), rape straw (0.42 mg/mL), rice straw (0.16 mg/mL), peanut straw (0.16 mg/mL), and corn straw (0.61 mg/mL). This study provides a potential additive in biomass saccharification applications.
abstractGer	Cellulase has been widely used in many industrial fields, such as feed and food industry, because it can hydrolyze cellulose to oligosaccharides with a lower degree of polymerization. Endo-β-1,4-glucanase is a critical speed-limiting cellulase in the saccharification process. In this study, endo-β-1,4-glucanase gene (<i<CelA257</i<) from <i<Myxococcus</i< sp. B6-1 was cloned and expressed in <i<Escherichia coli</i<. CelA257 contained carbohydrate-binding module (CBM) 4-9 and glycosyl hydrolase (GH) family 6 domain that shares 54.7% identity with endoglucanase from <i<Streptomyces halstedii</i<. The recombinant enzyme exhibited optimal activity at pH 6.5 and 50 °C and was stable over a broad pH (6–9.5) range and temperature < 50 °C. CelA257 exhibited broad substrate specificity to barley β-glucan, lichenin, CMC, chitosan, laminarin, avicel, and phosphoric acid swollen cellulose (PASC). CelA257 degraded both cellotetrose (G<sub<4</sub<) and cellppentaose (G<sub<5</sub<) to cellobiose (G<sub<2</sub<) and cellotriose (G<sub<3</sub<). Adding CelA257 increased the release of reducing sugars in crop straw powers, including wheat straw (0.18 mg/mL), rape straw (0.42 mg/mL), rice straw (0.16 mg/mL), peanut straw (0.16 mg/mL), and corn straw (0.61 mg/mL). This study provides a potential additive in biomass saccharification applications.
abstract_unstemmed	Cellulase has been widely used in many industrial fields, such as feed and food industry, because it can hydrolyze cellulose to oligosaccharides with a lower degree of polymerization. Endo-β-1,4-glucanase is a critical speed-limiting cellulase in the saccharification process. In this study, endo-β-1,4-glucanase gene (<i<CelA257</i<) from <i<Myxococcus</i< sp. B6-1 was cloned and expressed in <i<Escherichia coli</i<. CelA257 contained carbohydrate-binding module (CBM) 4-9 and glycosyl hydrolase (GH) family 6 domain that shares 54.7% identity with endoglucanase from <i<Streptomyces halstedii</i<. The recombinant enzyme exhibited optimal activity at pH 6.5 and 50 °C and was stable over a broad pH (6–9.5) range and temperature < 50 °C. CelA257 exhibited broad substrate specificity to barley β-glucan, lichenin, CMC, chitosan, laminarin, avicel, and phosphoric acid swollen cellulose (PASC). CelA257 degraded both cellotetrose (G<sub<4</sub<) and cellppentaose (G<sub<5</sub<) to cellobiose (G<sub<2</sub<) and cellotriose (G<sub<3</sub<). Adding CelA257 increased the release of reducing sugars in crop straw powers, including wheat straw (0.18 mg/mL), rape straw (0.42 mg/mL), rice straw (0.16 mg/mL), peanut straw (0.16 mg/mL), and corn straw (0.61 mg/mL). This study provides a potential additive in biomass saccharification applications.
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title_short	Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification
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up_date	2024-07-03T20:46:11.487Z
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Biochemical Characterization of Novel GH6 Endoglucanase from <i<Myxococcus</i< sp. B6-1 and Its Effects on Agricultural Straws Saccharification

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