Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage

Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid &...
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Autor*in:	Biran Gong [verfasserIn] Lei Zhao [verfasserIn] Chunyan Zeng [verfasserIn] Wei Zhu [verfasserIn] Lili Xu [verfasserIn] Dandan Wu [verfasserIn] Yiran Cheng [verfasserIn] Yi Wang [verfasserIn] Jian Zeng [verfasserIn] Xing Fan [verfasserIn] Lina Sha [verfasserIn] Haiqin Zhang [verfasserIn] Guoyue Chen [verfasserIn] Yonghong Zhou [verfasserIn] Houyang Kang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	chromosomal substitution molecular markers stripe rust tetraploid

Übergeordnetes Werk:	In: Plants - MDPI AG, 2013, 12(2023), 12, p 2311
Übergeordnetes Werk:	volume:12 ; year:2023 ; number:12, p 2311

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/plants12122311

Katalog-ID:	DOAJ094076499

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allfields	10.3390/plants12122311 doi (DE-627)DOAJ094076499 (DE-599)DOAJ5dbd20ceaf2543888265f310acddb9fc DE-627 ger DE-627 rakwb eng QK1-989 Biran Gong verfasserin aut Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i<Thinopyrum elongatum</i< (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i<Th. elongatum</i< 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i<Th. elongatum</i<, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i<Th. elongatum,</i< which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i<Th. elongatum</i< from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i<Th. elongatum</i<. chromosomal substitution molecular markers stripe rust tetraploid <i<Thinopyrum elongatum</i< Botany Lei Zhao verfasserin aut Chunyan Zeng verfasserin aut Wei Zhu verfasserin aut Lili Xu verfasserin aut Dandan Wu verfasserin aut Yiran Cheng verfasserin aut Yi Wang verfasserin aut Jian Zeng verfasserin aut Xing Fan verfasserin aut Lina Sha verfasserin aut Haiqin Zhang verfasserin aut Guoyue Chen verfasserin aut Yonghong Zhou verfasserin aut Houyang Kang verfasserin aut In Plants MDPI AG, 2013 12(2023), 12, p 2311 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:12, p 2311 https://doi.org/10.3390/plants12122311 kostenfrei https://doaj.org/article/5dbd20ceaf2543888265f310acddb9fc kostenfrei https://www.mdpi.com/2223-7747/12/12/2311 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 12, p 2311
spelling	10.3390/plants12122311 doi (DE-627)DOAJ094076499 (DE-599)DOAJ5dbd20ceaf2543888265f310acddb9fc DE-627 ger DE-627 rakwb eng QK1-989 Biran Gong verfasserin aut Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i<Thinopyrum elongatum</i< (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i<Th. elongatum</i< 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i<Th. elongatum</i<, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i<Th. elongatum,</i< which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i<Th. elongatum</i< from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i<Th. elongatum</i<. chromosomal substitution molecular markers stripe rust tetraploid <i<Thinopyrum elongatum</i< Botany Lei Zhao verfasserin aut Chunyan Zeng verfasserin aut Wei Zhu verfasserin aut Lili Xu verfasserin aut Dandan Wu verfasserin aut Yiran Cheng verfasserin aut Yi Wang verfasserin aut Jian Zeng verfasserin aut Xing Fan verfasserin aut Lina Sha verfasserin aut Haiqin Zhang verfasserin aut Guoyue Chen verfasserin aut Yonghong Zhou verfasserin aut Houyang Kang verfasserin aut In Plants MDPI AG, 2013 12(2023), 12, p 2311 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:12, p 2311 https://doi.org/10.3390/plants12122311 kostenfrei https://doaj.org/article/5dbd20ceaf2543888265f310acddb9fc kostenfrei https://www.mdpi.com/2223-7747/12/12/2311 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 12, p 2311
allfields_unstemmed	10.3390/plants12122311 doi (DE-627)DOAJ094076499 (DE-599)DOAJ5dbd20ceaf2543888265f310acddb9fc DE-627 ger DE-627 rakwb eng QK1-989 Biran Gong verfasserin aut Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i<Thinopyrum elongatum</i< (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i<Th. elongatum</i< 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i<Th. elongatum</i<, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i<Th. elongatum,</i< which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i<Th. elongatum</i< from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i<Th. elongatum</i<. chromosomal substitution molecular markers stripe rust tetraploid <i<Thinopyrum elongatum</i< Botany Lei Zhao verfasserin aut Chunyan Zeng verfasserin aut Wei Zhu verfasserin aut Lili Xu verfasserin aut Dandan Wu verfasserin aut Yiran Cheng verfasserin aut Yi Wang verfasserin aut Jian Zeng verfasserin aut Xing Fan verfasserin aut Lina Sha verfasserin aut Haiqin Zhang verfasserin aut Guoyue Chen verfasserin aut Yonghong Zhou verfasserin aut Houyang Kang verfasserin aut In Plants MDPI AG, 2013 12(2023), 12, p 2311 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:12, p 2311 https://doi.org/10.3390/plants12122311 kostenfrei https://doaj.org/article/5dbd20ceaf2543888265f310acddb9fc kostenfrei https://www.mdpi.com/2223-7747/12/12/2311 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 12, p 2311
allfieldsGer	10.3390/plants12122311 doi (DE-627)DOAJ094076499 (DE-599)DOAJ5dbd20ceaf2543888265f310acddb9fc DE-627 ger DE-627 rakwb eng QK1-989 Biran Gong verfasserin aut Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i<Thinopyrum elongatum</i< (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i<Th. elongatum</i< 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i<Th. elongatum</i<, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i<Th. elongatum,</i< which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i<Th. elongatum</i< from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i<Th. elongatum</i<. chromosomal substitution molecular markers stripe rust tetraploid <i<Thinopyrum elongatum</i< Botany Lei Zhao verfasserin aut Chunyan Zeng verfasserin aut Wei Zhu verfasserin aut Lili Xu verfasserin aut Dandan Wu verfasserin aut Yiran Cheng verfasserin aut Yi Wang verfasserin aut Jian Zeng verfasserin aut Xing Fan verfasserin aut Lina Sha verfasserin aut Haiqin Zhang verfasserin aut Guoyue Chen verfasserin aut Yonghong Zhou verfasserin aut Houyang Kang verfasserin aut In Plants MDPI AG, 2013 12(2023), 12, p 2311 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:12, p 2311 https://doi.org/10.3390/plants12122311 kostenfrei https://doaj.org/article/5dbd20ceaf2543888265f310acddb9fc kostenfrei https://www.mdpi.com/2223-7747/12/12/2311 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 12, p 2311
allfieldsSound	10.3390/plants12122311 doi (DE-627)DOAJ094076499 (DE-599)DOAJ5dbd20ceaf2543888265f310acddb9fc DE-627 ger DE-627 rakwb eng QK1-989 Biran Gong verfasserin aut Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i<Thinopyrum elongatum</i< (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i<Th. elongatum</i< 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i<Th. elongatum</i<, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i<Th. elongatum,</i< which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i<Th. elongatum</i< from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i<Th. elongatum</i<. chromosomal substitution molecular markers stripe rust tetraploid <i<Thinopyrum elongatum</i< Botany Lei Zhao verfasserin aut Chunyan Zeng verfasserin aut Wei Zhu verfasserin aut Lili Xu verfasserin aut Dandan Wu verfasserin aut Yiran Cheng verfasserin aut Yi Wang verfasserin aut Jian Zeng verfasserin aut Xing Fan verfasserin aut Lina Sha verfasserin aut Haiqin Zhang verfasserin aut Guoyue Chen verfasserin aut Yonghong Zhou verfasserin aut Houyang Kang verfasserin aut In Plants MDPI AG, 2013 12(2023), 12, p 2311 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:12 year:2023 number:12, p 2311 https://doi.org/10.3390/plants12122311 kostenfrei https://doaj.org/article/5dbd20ceaf2543888265f310acddb9fc kostenfrei https://www.mdpi.com/2223-7747/12/12/2311 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 12, p 2311
language	English
source	In Plants 12(2023), 12, p 2311 volume:12 year:2023 number:12, p 2311
sourceStr	In Plants 12(2023), 12, p 2311 volume:12 year:2023 number:12, p 2311
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	chromosomal substitution molecular markers stripe rust tetraploid <i<Thinopyrum elongatum</i< Botany
isfreeaccess_bool	true
container_title	Plants
authorswithroles_txt_mv	Biran Gong @@aut@@ Lei Zhao @@aut@@ Chunyan Zeng @@aut@@ Wei Zhu @@aut@@ Lili Xu @@aut@@ Dandan Wu @@aut@@ Yiran Cheng @@aut@@ Yi Wang @@aut@@ Jian Zeng @@aut@@ Xing Fan @@aut@@ Lina Sha @@aut@@ Haiqin Zhang @@aut@@ Guoyue Chen @@aut@@ Yonghong Zhou @@aut@@ Houyang Kang @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	737288345
id	DOAJ094076499
language_de	englisch
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callnumber-first	Q - Science
author	Biran Gong
spellingShingle	Biran Gong misc QK1-989 misc chromosomal substitution misc molecular markers misc stripe rust misc tetraploid <i<Thinopyrum elongatum</i< misc Botany Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
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topic_title	QK1-989 Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage chromosomal substitution molecular markers stripe rust tetraploid <i<Thinopyrum elongatum</i<
topic	misc QK1-989 misc chromosomal substitution misc molecular markers misc stripe rust misc tetraploid <i<Thinopyrum elongatum</i< misc Botany
topic_unstemmed	misc QK1-989 misc chromosomal substitution misc molecular markers misc stripe rust misc tetraploid <i<Thinopyrum elongatum</i< misc Botany
topic_browse	misc QK1-989 misc chromosomal substitution misc molecular markers misc stripe rust misc tetraploid <i<Thinopyrum elongatum</i< misc Botany
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title	Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
ctrlnum	(DE-627)DOAJ094076499 (DE-599)DOAJ5dbd20ceaf2543888265f310acddb9fc
title_full	Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
author_sort	Biran Gong
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author_browse	Biran Gong Lei Zhao Chunyan Zeng Wei Zhu Lili Xu Dandan Wu Yiran Cheng Yi Wang Jian Zeng Xing Fan Lina Sha Haiqin Zhang Guoyue Chen Yonghong Zhou Houyang Kang
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title_sort	development and characterization of a novel wheat–tetraploid <i<thinopyrum elongatum</i< 6e (6d) disomic substitution line with stripe rust resistance at the adult stage
callnumber	QK1-989
title_auth	Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
abstract	Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i<Thinopyrum elongatum</i< (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i<Th. elongatum</i< 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i<Th. elongatum</i<, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i<Th. elongatum,</i< which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i<Th. elongatum</i< from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i<Th. elongatum</i<.
abstractGer	Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i<Thinopyrum elongatum</i< (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i<Th. elongatum</i< 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i<Th. elongatum</i<, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i<Th. elongatum,</i< which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i<Th. elongatum</i< from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i<Th. elongatum</i<.
abstract_unstemmed	Stripe rust, which is caused by <i<Puccinia striiformis</i< f. sp. <i<tritici</i<, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i<Thinopyrum elongatum</i< (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i<Th. elongatum</i< 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i<Th. elongatum</i<, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i<Th. elongatum,</i< which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i<Th. elongatum</i< from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i<Th. elongatum</i<.
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container_issue	12, p 2311
title_short	Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
url	https://doi.org/10.3390/plants12122311 https://doaj.org/article/5dbd20ceaf2543888265f310acddb9fc https://www.mdpi.com/2223-7747/12/12/2311 https://doaj.org/toc/2223-7747
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author2	Lei Zhao Chunyan Zeng Wei Zhu Lili Xu Dandan Wu Yiran Cheng Yi Wang Jian Zeng Xing Fan Lina Sha Haiqin Zhang Guoyue Chen Yonghong Zhou Houyang Kang
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Development and Characterization of a Novel Wheat–Tetraploid <i<Thinopyrum elongatum</i< 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage

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