Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of <i<Maerua crassifolia</i< Forssk

The difficult propagation of shrub and tree species and their extensive exposure to grazing threaten their abundance and lead to the necessity to find alternative means of propagation for these species. In vitro micropropagation techniques, viz., tissue culture, offer a promising tool for the rapid,...
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Autor*in:	Abdulrahman A. Alatar [verfasserIn] Ahmed A. Qahtan [verfasserIn] Eslam M. Abdel-Salam [verfasserIn] Mohammad Faisal [verfasserIn] Mohamed A. El-Sheikh [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	tissue culture hypocotyls auxins cytokinins acclimatization flow cytometry

Übergeordnetes Werk:	In: Forests - MDPI AG, 2010, 14(2023), 6, p 1160
Übergeordnetes Werk:	volume:14 ; year:2023 ; number:6, p 1160

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/f14061160

Katalog-ID:	DOAJ094153574

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language	English
source	In Forests 14(2023), 6, p 1160 volume:14 year:2023 number:6, p 1160
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authorswithroles_txt_mv	Abdulrahman A. Alatar @@aut@@ Ahmed A. Qahtan @@aut@@ Eslam M. Abdel-Salam @@aut@@ Mohammad Faisal @@aut@@ Mohamed A. El-Sheikh @@aut@@
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callnumber-first	Q - Science
author	Abdulrahman A. Alatar
spellingShingle	Abdulrahman A. Alatar misc QK900-989 misc tissue culture misc hypocotyls misc auxins misc cytokinins misc acclimatization misc flow cytometry misc Plant ecology Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of <i<Maerua crassifolia</i< Forssk
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topic_title	QK900-989 Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of <i<Maerua crassifolia</i< Forssk tissue culture hypocotyls auxins cytokinins acclimatization flow cytometry
topic	misc QK900-989 misc tissue culture misc hypocotyls misc auxins misc cytokinins misc acclimatization misc flow cytometry misc Plant ecology
topic_unstemmed	misc QK900-989 misc tissue culture misc hypocotyls misc auxins misc cytokinins misc acclimatization misc flow cytometry misc Plant ecology
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title	Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of <i<Maerua crassifolia</i< Forssk
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title_full	Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of <i<Maerua crassifolia</i< Forssk
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title_sort	development of an efficient and rapid micropropagation protocol for in vitro multiplication of <i<maerua crassifolia</i< forssk
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title_auth	Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of <i<Maerua crassifolia</i< Forssk
abstract	The difficult propagation of shrub and tree species and their extensive exposure to grazing threaten their abundance and lead to the necessity to find alternative means of propagation for these species. In vitro micropropagation techniques, viz., tissue culture, offer a promising tool for the rapid, cost-effective, and efficient propagation of different plant species. In the current study, a rapid and efficient in vitro multiplication protocol was developed for the micropropagation of <i<Maerua crassifolia</i< Forssk. Our results revealed that Murashige and Skoog (MS) medium with 7.5 µM of 6-benzylaminopurine (BA) and 1.0 µM of 1-naphthaleneacetic acid (NAA) led to the highest shoot formation (13.9 shoots per explant in 85.7% of the cultivated hypocotyls) among all other treatments. The best in vitro root formation was obtained on half-strength MS medium with 1.0 µM of indole 3-butyric acid (IBA) as 94.1% of the cultivated shoots formed 6.8 roots per microshoot on average. Ninety percent of the rooted plantlets were successfully acclimatized and are currently growing in the botanical garden of the Botany and Microbiology Department, King Saud University, Riyadh, Saudi Arabia. The genetic fidelity of the micropropagated plants was authenticated via flow cytometry. The results of the current study explained a simple, cost-effective, and efficient protocol for the micropropagation of the endangered <i<M. crassifolia</i< trees.
abstractGer	The difficult propagation of shrub and tree species and their extensive exposure to grazing threaten their abundance and lead to the necessity to find alternative means of propagation for these species. In vitro micropropagation techniques, viz., tissue culture, offer a promising tool for the rapid, cost-effective, and efficient propagation of different plant species. In the current study, a rapid and efficient in vitro multiplication protocol was developed for the micropropagation of <i<Maerua crassifolia</i< Forssk. Our results revealed that Murashige and Skoog (MS) medium with 7.5 µM of 6-benzylaminopurine (BA) and 1.0 µM of 1-naphthaleneacetic acid (NAA) led to the highest shoot formation (13.9 shoots per explant in 85.7% of the cultivated hypocotyls) among all other treatments. The best in vitro root formation was obtained on half-strength MS medium with 1.0 µM of indole 3-butyric acid (IBA) as 94.1% of the cultivated shoots formed 6.8 roots per microshoot on average. Ninety percent of the rooted plantlets were successfully acclimatized and are currently growing in the botanical garden of the Botany and Microbiology Department, King Saud University, Riyadh, Saudi Arabia. The genetic fidelity of the micropropagated plants was authenticated via flow cytometry. The results of the current study explained a simple, cost-effective, and efficient protocol for the micropropagation of the endangered <i<M. crassifolia</i< trees.
abstract_unstemmed	The difficult propagation of shrub and tree species and their extensive exposure to grazing threaten their abundance and lead to the necessity to find alternative means of propagation for these species. In vitro micropropagation techniques, viz., tissue culture, offer a promising tool for the rapid, cost-effective, and efficient propagation of different plant species. In the current study, a rapid and efficient in vitro multiplication protocol was developed for the micropropagation of <i<Maerua crassifolia</i< Forssk. Our results revealed that Murashige and Skoog (MS) medium with 7.5 µM of 6-benzylaminopurine (BA) and 1.0 µM of 1-naphthaleneacetic acid (NAA) led to the highest shoot formation (13.9 shoots per explant in 85.7% of the cultivated hypocotyls) among all other treatments. The best in vitro root formation was obtained on half-strength MS medium with 1.0 µM of indole 3-butyric acid (IBA) as 94.1% of the cultivated shoots formed 6.8 roots per microshoot on average. Ninety percent of the rooted plantlets were successfully acclimatized and are currently growing in the botanical garden of the Botany and Microbiology Department, King Saud University, Riyadh, Saudi Arabia. The genetic fidelity of the micropropagated plants was authenticated via flow cytometry. The results of the current study explained a simple, cost-effective, and efficient protocol for the micropropagation of the endangered <i<M. crassifolia</i< trees.
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title_short	Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of <i<Maerua crassifolia</i< Forssk
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Development of an Efficient and Rapid Micropropagation Protocol for In Vitro Multiplication of <i<Maerua crassifolia</i< Forssk

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