<i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid

In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite o...
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Autor*in:	Malik Jan [verfasserIn] Sheng-Jie Yue [verfasserIn] Ru-Xiang Deng [verfasserIn] Yan-Fang Nie [verfasserIn] Hong-Yan Zhang [verfasserIn] Xiang-Rui Hao [verfasserIn] Wei Wang [verfasserIn] Hong-Bo Hu [verfasserIn] Xue-Hong Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	phenazine 1-carboxylic Acid biotransformation 3-hydroxy phenazine 1-carboxylic acid green chemistry

Übergeordnetes Werk:	In: Fermentation - MDPI AG, 2017, 9(2023), 6, p 579
Übergeordnetes Werk:	volume:9 ; year:2023 ; number:6, p 579

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/fermentation9060579

Katalog-ID:	DOAJ094157618

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spelling	10.3390/fermentation9060579 doi (DE-627)DOAJ094157618 (DE-599)DOAJ6e6333041623439fa05e3aab5e14b20d DE-627 ger DE-627 rakwb eng TP500-660 Malik Jan verfasserin aut <i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite of most phenazine derivatives in <i<Pseudomonas</i< spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain <i<Aspergillus sclerotiorum</i< was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was designed by screening optimal reaction conditions and carbon sources. Hence, <i<Aspergillus sclerotiorum</i< exhibited desirable adaptation by the consumption of different carbon sources and maximum whole-cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including a PCA concentration of 1120 mg/L, a pH of 7.0, a temperature of 25 °C, a rotation rate of 200 rpm, and dry cell weight of 15 g/L for 60 h; thus, 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of the repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle, but remained stable in all five cycles with the provision of a glucose supplement. These findings present the prospect of using filamentous fungi for the whole-cell biocatalysis of phenazine in enormous amounts and the efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic-based mechanism of the strain responsible for PCA biotransformation. <i<Aspergillus sclerotiorum</i< phenazine 1-carboxylic Acid biotransformation 3-hydroxy phenazine 1-carboxylic acid green chemistry Fermentation industries. Beverages. Alcohol Sheng-Jie Yue verfasserin aut Ru-Xiang Deng verfasserin aut Yan-Fang Nie verfasserin aut Hong-Yan Zhang verfasserin aut Xiang-Rui Hao verfasserin aut Wei Wang verfasserin aut Hong-Bo Hu verfasserin aut Xue-Hong Zhang verfasserin aut In Fermentation MDPI AG, 2017 9(2023), 6, p 579 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:9 year:2023 number:6, p 579 https://doi.org/10.3390/fermentation9060579 kostenfrei https://doaj.org/article/6e6333041623439fa05e3aab5e14b20d kostenfrei https://www.mdpi.com/2311-5637/9/6/579 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 6, p 579
allfields_unstemmed	10.3390/fermentation9060579 doi (DE-627)DOAJ094157618 (DE-599)DOAJ6e6333041623439fa05e3aab5e14b20d DE-627 ger DE-627 rakwb eng TP500-660 Malik Jan verfasserin aut <i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite of most phenazine derivatives in <i<Pseudomonas</i< spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain <i<Aspergillus sclerotiorum</i< was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was designed by screening optimal reaction conditions and carbon sources. Hence, <i<Aspergillus sclerotiorum</i< exhibited desirable adaptation by the consumption of different carbon sources and maximum whole-cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including a PCA concentration of 1120 mg/L, a pH of 7.0, a temperature of 25 °C, a rotation rate of 200 rpm, and dry cell weight of 15 g/L for 60 h; thus, 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of the repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle, but remained stable in all five cycles with the provision of a glucose supplement. These findings present the prospect of using filamentous fungi for the whole-cell biocatalysis of phenazine in enormous amounts and the efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic-based mechanism of the strain responsible for PCA biotransformation. <i<Aspergillus sclerotiorum</i< phenazine 1-carboxylic Acid biotransformation 3-hydroxy phenazine 1-carboxylic acid green chemistry Fermentation industries. Beverages. Alcohol Sheng-Jie Yue verfasserin aut Ru-Xiang Deng verfasserin aut Yan-Fang Nie verfasserin aut Hong-Yan Zhang verfasserin aut Xiang-Rui Hao verfasserin aut Wei Wang verfasserin aut Hong-Bo Hu verfasserin aut Xue-Hong Zhang verfasserin aut In Fermentation MDPI AG, 2017 9(2023), 6, p 579 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:9 year:2023 number:6, p 579 https://doi.org/10.3390/fermentation9060579 kostenfrei https://doaj.org/article/6e6333041623439fa05e3aab5e14b20d kostenfrei https://www.mdpi.com/2311-5637/9/6/579 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 6, p 579
allfieldsGer	10.3390/fermentation9060579 doi (DE-627)DOAJ094157618 (DE-599)DOAJ6e6333041623439fa05e3aab5e14b20d DE-627 ger DE-627 rakwb eng TP500-660 Malik Jan verfasserin aut <i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite of most phenazine derivatives in <i<Pseudomonas</i< spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain <i<Aspergillus sclerotiorum</i< was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was designed by screening optimal reaction conditions and carbon sources. Hence, <i<Aspergillus sclerotiorum</i< exhibited desirable adaptation by the consumption of different carbon sources and maximum whole-cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including a PCA concentration of 1120 mg/L, a pH of 7.0, a temperature of 25 °C, a rotation rate of 200 rpm, and dry cell weight of 15 g/L for 60 h; thus, 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of the repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle, but remained stable in all five cycles with the provision of a glucose supplement. These findings present the prospect of using filamentous fungi for the whole-cell biocatalysis of phenazine in enormous amounts and the efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic-based mechanism of the strain responsible for PCA biotransformation. <i<Aspergillus sclerotiorum</i< phenazine 1-carboxylic Acid biotransformation 3-hydroxy phenazine 1-carboxylic acid green chemistry Fermentation industries. Beverages. Alcohol Sheng-Jie Yue verfasserin aut Ru-Xiang Deng verfasserin aut Yan-Fang Nie verfasserin aut Hong-Yan Zhang verfasserin aut Xiang-Rui Hao verfasserin aut Wei Wang verfasserin aut Hong-Bo Hu verfasserin aut Xue-Hong Zhang verfasserin aut In Fermentation MDPI AG, 2017 9(2023), 6, p 579 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:9 year:2023 number:6, p 579 https://doi.org/10.3390/fermentation9060579 kostenfrei https://doaj.org/article/6e6333041623439fa05e3aab5e14b20d kostenfrei https://www.mdpi.com/2311-5637/9/6/579 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 6, p 579
allfieldsSound	10.3390/fermentation9060579 doi (DE-627)DOAJ094157618 (DE-599)DOAJ6e6333041623439fa05e3aab5e14b20d DE-627 ger DE-627 rakwb eng TP500-660 Malik Jan verfasserin aut <i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite of most phenazine derivatives in <i<Pseudomonas</i< spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain <i<Aspergillus sclerotiorum</i< was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was designed by screening optimal reaction conditions and carbon sources. Hence, <i<Aspergillus sclerotiorum</i< exhibited desirable adaptation by the consumption of different carbon sources and maximum whole-cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including a PCA concentration of 1120 mg/L, a pH of 7.0, a temperature of 25 °C, a rotation rate of 200 rpm, and dry cell weight of 15 g/L for 60 h; thus, 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of the repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle, but remained stable in all five cycles with the provision of a glucose supplement. These findings present the prospect of using filamentous fungi for the whole-cell biocatalysis of phenazine in enormous amounts and the efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic-based mechanism of the strain responsible for PCA biotransformation. <i<Aspergillus sclerotiorum</i< phenazine 1-carboxylic Acid biotransformation 3-hydroxy phenazine 1-carboxylic acid green chemistry Fermentation industries. Beverages. Alcohol Sheng-Jie Yue verfasserin aut Ru-Xiang Deng verfasserin aut Yan-Fang Nie verfasserin aut Hong-Yan Zhang verfasserin aut Xiang-Rui Hao verfasserin aut Wei Wang verfasserin aut Hong-Bo Hu verfasserin aut Xue-Hong Zhang verfasserin aut In Fermentation MDPI AG, 2017 9(2023), 6, p 579 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:9 year:2023 number:6, p 579 https://doi.org/10.3390/fermentation9060579 kostenfrei https://doaj.org/article/6e6333041623439fa05e3aab5e14b20d kostenfrei https://www.mdpi.com/2311-5637/9/6/579 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2023 6, p 579
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topic_title	TP500-660 <i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid <i<Aspergillus sclerotiorum</i< phenazine 1-carboxylic Acid biotransformation 3-hydroxy phenazine 1-carboxylic acid green chemistry
topic	misc TP500-660 misc <i<Aspergillus sclerotiorum</i< misc phenazine 1-carboxylic Acid misc biotransformation misc 3-hydroxy phenazine 1-carboxylic acid misc green chemistry misc Fermentation industries. Beverages. Alcohol
topic_unstemmed	misc TP500-660 misc <i<Aspergillus sclerotiorum</i< misc phenazine 1-carboxylic Acid misc biotransformation misc 3-hydroxy phenazine 1-carboxylic acid misc green chemistry misc Fermentation industries. Beverages. Alcohol
topic_browse	misc TP500-660 misc <i<Aspergillus sclerotiorum</i< misc phenazine 1-carboxylic Acid misc biotransformation misc 3-hydroxy phenazine 1-carboxylic acid misc green chemistry misc Fermentation industries. Beverages. Alcohol
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title	<i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid
ctrlnum	(DE-627)DOAJ094157618 (DE-599)DOAJ6e6333041623439fa05e3aab5e14b20d
title_full	<i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid
author_sort	Malik Jan
journal	Fermentation
journalStr	Fermentation
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author_browse	Malik Jan Sheng-Jie Yue Ru-Xiang Deng Yan-Fang Nie Hong-Yan Zhang Xiang-Rui Hao Wei Wang Hong-Bo Hu Xue-Hong Zhang
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doi_str_mv	10.3390/fermentation9060579
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title_sort	<i<aspergillus sclerotiorum</i< whole-cell biocatalysis: a sustainable approach to produce 3-hydroxy-phenazine 1-carboxylic acid from phenazine 1-carboxylic acid
callnumber	TP500-660
title_auth	<i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid
abstract	In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite of most phenazine derivatives in <i<Pseudomonas</i< spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain <i<Aspergillus sclerotiorum</i< was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was designed by screening optimal reaction conditions and carbon sources. Hence, <i<Aspergillus sclerotiorum</i< exhibited desirable adaptation by the consumption of different carbon sources and maximum whole-cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including a PCA concentration of 1120 mg/L, a pH of 7.0, a temperature of 25 °C, a rotation rate of 200 rpm, and dry cell weight of 15 g/L for 60 h; thus, 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of the repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle, but remained stable in all five cycles with the provision of a glucose supplement. These findings present the prospect of using filamentous fungi for the whole-cell biocatalysis of phenazine in enormous amounts and the efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic-based mechanism of the strain responsible for PCA biotransformation.
abstractGer	In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite of most phenazine derivatives in <i<Pseudomonas</i< spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain <i<Aspergillus sclerotiorum</i< was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was designed by screening optimal reaction conditions and carbon sources. Hence, <i<Aspergillus sclerotiorum</i< exhibited desirable adaptation by the consumption of different carbon sources and maximum whole-cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including a PCA concentration of 1120 mg/L, a pH of 7.0, a temperature of 25 °C, a rotation rate of 200 rpm, and dry cell weight of 15 g/L for 60 h; thus, 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of the repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle, but remained stable in all five cycles with the provision of a glucose supplement. These findings present the prospect of using filamentous fungi for the whole-cell biocatalysis of phenazine in enormous amounts and the efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic-based mechanism of the strain responsible for PCA biotransformation.
abstract_unstemmed	In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite of most phenazine derivatives in <i<Pseudomonas</i< spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain <i<Aspergillus sclerotiorum</i< was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was designed by screening optimal reaction conditions and carbon sources. Hence, <i<Aspergillus sclerotiorum</i< exhibited desirable adaptation by the consumption of different carbon sources and maximum whole-cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including a PCA concentration of 1120 mg/L, a pH of 7.0, a temperature of 25 °C, a rotation rate of 200 rpm, and dry cell weight of 15 g/L for 60 h; thus, 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of the repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle, but remained stable in all five cycles with the provision of a glucose supplement. These findings present the prospect of using filamentous fungi for the whole-cell biocatalysis of phenazine in enormous amounts and the efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic-based mechanism of the strain responsible for PCA biotransformation.
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container_issue	6, p 579
title_short	<i<Aspergillus sclerotiorum</i< Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid
url	https://doi.org/10.3390/fermentation9060579 https://doaj.org/article/6e6333041623439fa05e3aab5e14b20d https://www.mdpi.com/2311-5637/9/6/579 https://doaj.org/toc/2311-5637
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author2	Sheng-Jie Yue Ru-Xiang Deng Yan-Fang Nie Hong-Yan Zhang Xiang-Rui Hao Wei Wang Hong-Bo Hu Xue-Hong Zhang
author2Str	Sheng-Jie Yue Ru-Xiang Deng Yan-Fang Nie Hong-Yan Zhang Xiang-Rui Hao Wei Wang Hong-Bo Hu Xue-Hong Zhang
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up_date	2024-07-03T21:35:42.107Z
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