Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells

Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Poorya Rasekh [verfasserIn] Ali Kameli [verfasserIn] Arezoo Khoradmehr [verfasserIn] Neda Baghban [verfasserIn] Gholamhossein Mohebbi [verfasserIn] Alireza Barmak [verfasserIn] Iraj Nabipour [verfasserIn] Hossein Azari [verfasserIn] Yaser Heidari [verfasserIn] Adel Daneshi [verfasserIn] Afshar Bargahi [verfasserIn] Zahra Khodabandeh [verfasserIn] Shahrokh Zare [verfasserIn] Alireza Afshar [verfasserIn] Reza Shirazi [verfasserIn] Sahar Almasi-Turk [verfasserIn] Amin Tamadon [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	stem cells sea cucumbers proliferation aqueous extract bioactive compounds epidermal growth factor

Übergeordnetes Werk:	In: Marine Drugs - MDPI AG, 2005, 21(2023), 5, p 267
Übergeordnetes Werk:	volume:21 ; year:2023 ; number:5, p 267

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/md21050267

Katalog-ID:	DOAJ09434969X

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520			\|a Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs.
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allfields	10.3390/md21050267 doi (DE-627)DOAJ09434969X (DE-599)DOAJ12e49eba6fb94845a7242d740251e684 DE-627 ger DE-627 rakwb eng QH301-705.5 Poorya Rasekh verfasserin aut Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs. stem cells sea cucumbers proliferation aqueous extract bioactive compounds epidermal growth factor Biology (General) Ali Kameli verfasserin aut Arezoo Khoradmehr verfasserin aut Neda Baghban verfasserin aut Gholamhossein Mohebbi verfasserin aut Alireza Barmak verfasserin aut Iraj Nabipour verfasserin aut Hossein Azari verfasserin aut Yaser Heidari verfasserin aut Adel Daneshi verfasserin aut Afshar Bargahi verfasserin aut Zahra Khodabandeh verfasserin aut Shahrokh Zare verfasserin aut Alireza Afshar verfasserin aut Reza Shirazi verfasserin aut Sahar Almasi-Turk verfasserin aut Amin Tamadon verfasserin aut In Marine Drugs MDPI AG, 2005 21(2023), 5, p 267 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:21 year:2023 number:5, p 267 https://doi.org/10.3390/md21050267 kostenfrei https://doaj.org/article/12e49eba6fb94845a7242d740251e684 kostenfrei https://www.mdpi.com/1660-3397/21/5/267 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 5, p 267
spelling	10.3390/md21050267 doi (DE-627)DOAJ09434969X (DE-599)DOAJ12e49eba6fb94845a7242d740251e684 DE-627 ger DE-627 rakwb eng QH301-705.5 Poorya Rasekh verfasserin aut Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs. stem cells sea cucumbers proliferation aqueous extract bioactive compounds epidermal growth factor Biology (General) Ali Kameli verfasserin aut Arezoo Khoradmehr verfasserin aut Neda Baghban verfasserin aut Gholamhossein Mohebbi verfasserin aut Alireza Barmak verfasserin aut Iraj Nabipour verfasserin aut Hossein Azari verfasserin aut Yaser Heidari verfasserin aut Adel Daneshi verfasserin aut Afshar Bargahi verfasserin aut Zahra Khodabandeh verfasserin aut Shahrokh Zare verfasserin aut Alireza Afshar verfasserin aut Reza Shirazi verfasserin aut Sahar Almasi-Turk verfasserin aut Amin Tamadon verfasserin aut In Marine Drugs MDPI AG, 2005 21(2023), 5, p 267 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:21 year:2023 number:5, p 267 https://doi.org/10.3390/md21050267 kostenfrei https://doaj.org/article/12e49eba6fb94845a7242d740251e684 kostenfrei https://www.mdpi.com/1660-3397/21/5/267 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 5, p 267
allfields_unstemmed	10.3390/md21050267 doi (DE-627)DOAJ09434969X (DE-599)DOAJ12e49eba6fb94845a7242d740251e684 DE-627 ger DE-627 rakwb eng QH301-705.5 Poorya Rasekh verfasserin aut Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs. stem cells sea cucumbers proliferation aqueous extract bioactive compounds epidermal growth factor Biology (General) Ali Kameli verfasserin aut Arezoo Khoradmehr verfasserin aut Neda Baghban verfasserin aut Gholamhossein Mohebbi verfasserin aut Alireza Barmak verfasserin aut Iraj Nabipour verfasserin aut Hossein Azari verfasserin aut Yaser Heidari verfasserin aut Adel Daneshi verfasserin aut Afshar Bargahi verfasserin aut Zahra Khodabandeh verfasserin aut Shahrokh Zare verfasserin aut Alireza Afshar verfasserin aut Reza Shirazi verfasserin aut Sahar Almasi-Turk verfasserin aut Amin Tamadon verfasserin aut In Marine Drugs MDPI AG, 2005 21(2023), 5, p 267 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:21 year:2023 number:5, p 267 https://doi.org/10.3390/md21050267 kostenfrei https://doaj.org/article/12e49eba6fb94845a7242d740251e684 kostenfrei https://www.mdpi.com/1660-3397/21/5/267 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 5, p 267
allfieldsGer	10.3390/md21050267 doi (DE-627)DOAJ09434969X (DE-599)DOAJ12e49eba6fb94845a7242d740251e684 DE-627 ger DE-627 rakwb eng QH301-705.5 Poorya Rasekh verfasserin aut Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs. stem cells sea cucumbers proliferation aqueous extract bioactive compounds epidermal growth factor Biology (General) Ali Kameli verfasserin aut Arezoo Khoradmehr verfasserin aut Neda Baghban verfasserin aut Gholamhossein Mohebbi verfasserin aut Alireza Barmak verfasserin aut Iraj Nabipour verfasserin aut Hossein Azari verfasserin aut Yaser Heidari verfasserin aut Adel Daneshi verfasserin aut Afshar Bargahi verfasserin aut Zahra Khodabandeh verfasserin aut Shahrokh Zare verfasserin aut Alireza Afshar verfasserin aut Reza Shirazi verfasserin aut Sahar Almasi-Turk verfasserin aut Amin Tamadon verfasserin aut In Marine Drugs MDPI AG, 2005 21(2023), 5, p 267 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:21 year:2023 number:5, p 267 https://doi.org/10.3390/md21050267 kostenfrei https://doaj.org/article/12e49eba6fb94845a7242d740251e684 kostenfrei https://www.mdpi.com/1660-3397/21/5/267 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 5, p 267
allfieldsSound	10.3390/md21050267 doi (DE-627)DOAJ09434969X (DE-599)DOAJ12e49eba6fb94845a7242d740251e684 DE-627 ger DE-627 rakwb eng QH301-705.5 Poorya Rasekh verfasserin aut Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs. stem cells sea cucumbers proliferation aqueous extract bioactive compounds epidermal growth factor Biology (General) Ali Kameli verfasserin aut Arezoo Khoradmehr verfasserin aut Neda Baghban verfasserin aut Gholamhossein Mohebbi verfasserin aut Alireza Barmak verfasserin aut Iraj Nabipour verfasserin aut Hossein Azari verfasserin aut Yaser Heidari verfasserin aut Adel Daneshi verfasserin aut Afshar Bargahi verfasserin aut Zahra Khodabandeh verfasserin aut Shahrokh Zare verfasserin aut Alireza Afshar verfasserin aut Reza Shirazi verfasserin aut Sahar Almasi-Turk verfasserin aut Amin Tamadon verfasserin aut In Marine Drugs MDPI AG, 2005 21(2023), 5, p 267 (DE-627)477992420 (DE-600)2175190-0 16603397 nnns volume:21 year:2023 number:5, p 267 https://doi.org/10.3390/md21050267 kostenfrei https://doaj.org/article/12e49eba6fb94845a7242d740251e684 kostenfrei https://www.mdpi.com/1660-3397/21/5/267 kostenfrei https://doaj.org/toc/1660-3397 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_381 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2023 5, p 267
language	English
source	In Marine Drugs 21(2023), 5, p 267 volume:21 year:2023 number:5, p 267
sourceStr	In Marine Drugs 21(2023), 5, p 267 volume:21 year:2023 number:5, p 267
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	stem cells sea cucumbers proliferation aqueous extract bioactive compounds epidermal growth factor Biology (General)
isfreeaccess_bool	true
container_title	Marine Drugs
authorswithroles_txt_mv	Poorya Rasekh @@aut@@ Ali Kameli @@aut@@ Arezoo Khoradmehr @@aut@@ Neda Baghban @@aut@@ Gholamhossein Mohebbi @@aut@@ Alireza Barmak @@aut@@ Iraj Nabipour @@aut@@ Hossein Azari @@aut@@ Yaser Heidari @@aut@@ Adel Daneshi @@aut@@ Afshar Bargahi @@aut@@ Zahra Khodabandeh @@aut@@ Shahrokh Zare @@aut@@ Alireza Afshar @@aut@@ Reza Shirazi @@aut@@ Sahar Almasi-Turk @@aut@@ Amin Tamadon @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	477992420
id	DOAJ09434969X
language_de	englisch
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callnumber-first	Q - Science
author	Poorya Rasekh
spellingShingle	Poorya Rasekh misc QH301-705.5 misc stem cells misc sea cucumbers misc proliferation misc aqueous extract misc bioactive compounds misc epidermal growth factor misc Biology (General) Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells
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topic_title	QH301-705.5 Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells stem cells sea cucumbers proliferation aqueous extract bioactive compounds epidermal growth factor
topic	misc QH301-705.5 misc stem cells misc sea cucumbers misc proliferation misc aqueous extract misc bioactive compounds misc epidermal growth factor misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc stem cells misc sea cucumbers misc proliferation misc aqueous extract misc bioactive compounds misc epidermal growth factor misc Biology (General)
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title	Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells
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title_full	Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells
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author_browse	Poorya Rasekh Ali Kameli Arezoo Khoradmehr Neda Baghban Gholamhossein Mohebbi Alireza Barmak Iraj Nabipour Hossein Azari Yaser Heidari Adel Daneshi Afshar Bargahi Zahra Khodabandeh Shahrokh Zare Alireza Afshar Reza Shirazi Sahar Almasi-Turk Amin Tamadon
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title_sort	proliferative effect of aqueous extract of sea cucumber (<i<holothuria parva</i<) body wall on human umbilical cord mesenchymal stromal/stem cells
callnumber	QH301-705.5
title_auth	Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells
abstract	Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs.
abstractGer	Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs.
abstract_unstemmed	Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of <i<Holothuria parva</i< body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of <i<H. parva</i<. The aqueous extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of <i<H. parva</i< and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of <i<H. parva</i<. A MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of <i<H. parva</i< had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The <i<H. parva</i< aqueous extract showed proliferative potential on hUC-MSCs.
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title_short	Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells
url	https://doi.org/10.3390/md21050267 https://doaj.org/article/12e49eba6fb94845a7242d740251e684 https://www.mdpi.com/1660-3397/21/5/267 https://doaj.org/toc/1660-3397
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The cell count, which was treated with a 20 µg/mL concentration, increased faster and higher than the control group (<i<p</i< < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs’ viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. 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Proliferative Effect of Aqueous Extract of Sea Cucumber (<i<Holothuria parva</i<) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells

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