Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation

Black phosphorus (BP), an emerging two-dimensional material with unique optical, thermoelectric, and mechanical properties, has been proposed as bioactive material for tissue engineering. However, its toxic effects on physiological systems remain obscure. The present study investigated the cytotoxic...
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Autor*in:	Hao Dong [verfasserIn] Yin Wen [verfasserIn] Jiating Lin [verfasserIn] Xianxian Zhuang [verfasserIn] Ruoting Xian [verfasserIn] Ping Li [verfasserIn] Shaobing Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	nanomaterials black phosphorus cytotoxicity vascular endothelial cell reactive oxygen species apoptosis

Übergeordnetes Werk:	In: Journal of Functional Biomaterials - MDPI AG, 2011, 14(2023), 5, p 284
Übergeordnetes Werk:	volume:14 ; year:2023 ; number:5, p 284

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/jfb14050284

Katalog-ID:	DOAJ09435975X

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spelling	10.3390/jfb14050284 doi (DE-627)DOAJ09435975X (DE-599)DOAJf1676fa20d8f46b790b93ec5050932a4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 R5-920 Hao Dong verfasserin aut Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Black phosphorus (BP), an emerging two-dimensional material with unique optical, thermoelectric, and mechanical properties, has been proposed as bioactive material for tissue engineering. However, its toxic effects on physiological systems remain obscure. The present study investigated the cytotoxicity of BP to vascular endothelial cells. BP nanosheets (BPNSs) with a diameter of 230 nm were fabricated via a classical liquid-phase exfoliation method. Human umbilical vein endothelial cells (HUVECs) were used to determine the cytotoxicity induced by BPNSs (0.31–80 μg/mL). When the concentrations were over 2.5 μg/mL, BPNSs adversely affected the cytoskeleton and cell migration. Furthermore, BPNSs caused mitochondrial dysfunction and generated excessive intercellular reactive oxygen species (ROS) at tested concentrations after 24 h. BPNSs could influence the expression of apoptosis-related genes, including the P53 and BCL-2 family, resulting in the apoptosis of HUVECs. Therefore, the viability and function of HUVECs were adversely influenced by the concentration of BPNSs over 2.5 μg/mL. These findings provide significant information for the potential applications of BP in tissue engineering. nanomaterials black phosphorus cytotoxicity vascular endothelial cell reactive oxygen species apoptosis Biotechnology Medicine (General) Yin Wen verfasserin aut Jiating Lin verfasserin aut Xianxian Zhuang verfasserin aut Ruoting Xian verfasserin aut Ping Li verfasserin aut Shaobing Li verfasserin aut In Journal of Functional Biomaterials MDPI AG, 2011 14(2023), 5, p 284 (DE-627)68456775X (DE-600)2648525-4 20794983 nnns volume:14 year:2023 number:5, p 284 https://doi.org/10.3390/jfb14050284 kostenfrei https://doaj.org/article/f1676fa20d8f46b790b93ec5050932a4 kostenfrei https://www.mdpi.com/2079-4983/14/5/284 kostenfrei https://doaj.org/toc/2079-4983 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 5, p 284
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allfieldsSound	10.3390/jfb14050284 doi (DE-627)DOAJ09435975X (DE-599)DOAJf1676fa20d8f46b790b93ec5050932a4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 R5-920 Hao Dong verfasserin aut Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Black phosphorus (BP), an emerging two-dimensional material with unique optical, thermoelectric, and mechanical properties, has been proposed as bioactive material for tissue engineering. However, its toxic effects on physiological systems remain obscure. The present study investigated the cytotoxicity of BP to vascular endothelial cells. BP nanosheets (BPNSs) with a diameter of 230 nm were fabricated via a classical liquid-phase exfoliation method. Human umbilical vein endothelial cells (HUVECs) were used to determine the cytotoxicity induced by BPNSs (0.31–80 μg/mL). When the concentrations were over 2.5 μg/mL, BPNSs adversely affected the cytoskeleton and cell migration. Furthermore, BPNSs caused mitochondrial dysfunction and generated excessive intercellular reactive oxygen species (ROS) at tested concentrations after 24 h. BPNSs could influence the expression of apoptosis-related genes, including the P53 and BCL-2 family, resulting in the apoptosis of HUVECs. Therefore, the viability and function of HUVECs were adversely influenced by the concentration of BPNSs over 2.5 μg/mL. These findings provide significant information for the potential applications of BP in tissue engineering. nanomaterials black phosphorus cytotoxicity vascular endothelial cell reactive oxygen species apoptosis Biotechnology Medicine (General) Yin Wen verfasserin aut Jiating Lin verfasserin aut Xianxian Zhuang verfasserin aut Ruoting Xian verfasserin aut Ping Li verfasserin aut Shaobing Li verfasserin aut In Journal of Functional Biomaterials MDPI AG, 2011 14(2023), 5, p 284 (DE-627)68456775X (DE-600)2648525-4 20794983 nnns volume:14 year:2023 number:5, p 284 https://doi.org/10.3390/jfb14050284 kostenfrei https://doaj.org/article/f1676fa20d8f46b790b93ec5050932a4 kostenfrei https://www.mdpi.com/2079-4983/14/5/284 kostenfrei https://doaj.org/toc/2079-4983 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 5, p 284
language	English
source	In Journal of Functional Biomaterials 14(2023), 5, p 284 volume:14 year:2023 number:5, p 284
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topic_facet	nanomaterials black phosphorus cytotoxicity vascular endothelial cell reactive oxygen species apoptosis Biotechnology Medicine (General)
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authorswithroles_txt_mv	Hao Dong @@aut@@ Yin Wen @@aut@@ Jiating Lin @@aut@@ Xianxian Zhuang @@aut@@ Ruoting Xian @@aut@@ Ping Li @@aut@@ Shaobing Li @@aut@@
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callnumber-first	T - Technology
author	Hao Dong
spellingShingle	Hao Dong misc TP248.13-248.65 misc R5-920 misc nanomaterials misc black phosphorus misc cytotoxicity misc vascular endothelial cell misc reactive oxygen species misc apoptosis misc Biotechnology misc Medicine (General) Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation
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topic_title	TP248.13-248.65 R5-920 Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation nanomaterials black phosphorus cytotoxicity vascular endothelial cell reactive oxygen species apoptosis
topic	misc TP248.13-248.65 misc R5-920 misc nanomaterials misc black phosphorus misc cytotoxicity misc vascular endothelial cell misc reactive oxygen species misc apoptosis misc Biotechnology misc Medicine (General)
topic_unstemmed	misc TP248.13-248.65 misc R5-920 misc nanomaterials misc black phosphorus misc cytotoxicity misc vascular endothelial cell misc reactive oxygen species misc apoptosis misc Biotechnology misc Medicine (General)
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title	Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation
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title_full	Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation
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author_browse	Hao Dong Yin Wen Jiating Lin Xianxian Zhuang Ruoting Xian Ping Li Shaobing Li
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title_sort	cytotoxicity induced by black phosphorus nanosheets in vascular endothelial cells via oxidative stress and apoptosis activation
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title_auth	Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation
abstract	Black phosphorus (BP), an emerging two-dimensional material with unique optical, thermoelectric, and mechanical properties, has been proposed as bioactive material for tissue engineering. However, its toxic effects on physiological systems remain obscure. The present study investigated the cytotoxicity of BP to vascular endothelial cells. BP nanosheets (BPNSs) with a diameter of 230 nm were fabricated via a classical liquid-phase exfoliation method. Human umbilical vein endothelial cells (HUVECs) were used to determine the cytotoxicity induced by BPNSs (0.31–80 μg/mL). When the concentrations were over 2.5 μg/mL, BPNSs adversely affected the cytoskeleton and cell migration. Furthermore, BPNSs caused mitochondrial dysfunction and generated excessive intercellular reactive oxygen species (ROS) at tested concentrations after 24 h. BPNSs could influence the expression of apoptosis-related genes, including the P53 and BCL-2 family, resulting in the apoptosis of HUVECs. Therefore, the viability and function of HUVECs were adversely influenced by the concentration of BPNSs over 2.5 μg/mL. These findings provide significant information for the potential applications of BP in tissue engineering.
abstractGer	Black phosphorus (BP), an emerging two-dimensional material with unique optical, thermoelectric, and mechanical properties, has been proposed as bioactive material for tissue engineering. However, its toxic effects on physiological systems remain obscure. The present study investigated the cytotoxicity of BP to vascular endothelial cells. BP nanosheets (BPNSs) with a diameter of 230 nm were fabricated via a classical liquid-phase exfoliation method. Human umbilical vein endothelial cells (HUVECs) were used to determine the cytotoxicity induced by BPNSs (0.31–80 μg/mL). When the concentrations were over 2.5 μg/mL, BPNSs adversely affected the cytoskeleton and cell migration. Furthermore, BPNSs caused mitochondrial dysfunction and generated excessive intercellular reactive oxygen species (ROS) at tested concentrations after 24 h. BPNSs could influence the expression of apoptosis-related genes, including the P53 and BCL-2 family, resulting in the apoptosis of HUVECs. Therefore, the viability and function of HUVECs were adversely influenced by the concentration of BPNSs over 2.5 μg/mL. These findings provide significant information for the potential applications of BP in tissue engineering.
abstract_unstemmed	Black phosphorus (BP), an emerging two-dimensional material with unique optical, thermoelectric, and mechanical properties, has been proposed as bioactive material for tissue engineering. However, its toxic effects on physiological systems remain obscure. The present study investigated the cytotoxicity of BP to vascular endothelial cells. BP nanosheets (BPNSs) with a diameter of 230 nm were fabricated via a classical liquid-phase exfoliation method. Human umbilical vein endothelial cells (HUVECs) were used to determine the cytotoxicity induced by BPNSs (0.31–80 μg/mL). When the concentrations were over 2.5 μg/mL, BPNSs adversely affected the cytoskeleton and cell migration. Furthermore, BPNSs caused mitochondrial dysfunction and generated excessive intercellular reactive oxygen species (ROS) at tested concentrations after 24 h. BPNSs could influence the expression of apoptosis-related genes, including the P53 and BCL-2 family, resulting in the apoptosis of HUVECs. Therefore, the viability and function of HUVECs were adversely influenced by the concentration of BPNSs over 2.5 μg/mL. These findings provide significant information for the potential applications of BP in tissue engineering.
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title_short	Cytotoxicity Induced by Black Phosphorus Nanosheets in Vascular Endothelial Cells via Oxidative Stress and Apoptosis Activation
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