Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches

Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle...
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Autor*in:	Valeria Cornelius [verfasserIn] Linda Droessler [verfasserIn] Salah Amasheh [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Peyer’s patch quercetin tight junction epithelial barrier Ussing chamber claudins

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 25(2024), 3, p 1530
Übergeordnetes Werk:	volume:25 ; year:2024 ; number:3, p 1530

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms25031530

Katalog-ID:	DOAJ094494142

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520			\|a Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE.
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allfields	10.3390/ijms25031530 doi (DE-627)DOAJ094494142 (DE-599)DOAJfb102ca6d8cc401ea0d314d2aa84bb29 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Valeria Cornelius verfasserin aut Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE. Peyer’s patch quercetin tight junction epithelial barrier Ussing chamber claudins Biology (General) Chemistry Linda Droessler verfasserin aut Salah Amasheh verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 3, p 1530 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:3, p 1530 https://doi.org/10.3390/ijms25031530 kostenfrei https://doaj.org/article/fb102ca6d8cc401ea0d314d2aa84bb29 kostenfrei https://www.mdpi.com/1422-0067/25/3/1530 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 3, p 1530
spelling	10.3390/ijms25031530 doi (DE-627)DOAJ094494142 (DE-599)DOAJfb102ca6d8cc401ea0d314d2aa84bb29 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Valeria Cornelius verfasserin aut Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE. Peyer’s patch quercetin tight junction epithelial barrier Ussing chamber claudins Biology (General) Chemistry Linda Droessler verfasserin aut Salah Amasheh verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 3, p 1530 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:3, p 1530 https://doi.org/10.3390/ijms25031530 kostenfrei https://doaj.org/article/fb102ca6d8cc401ea0d314d2aa84bb29 kostenfrei https://www.mdpi.com/1422-0067/25/3/1530 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 3, p 1530
allfields_unstemmed	10.3390/ijms25031530 doi (DE-627)DOAJ094494142 (DE-599)DOAJfb102ca6d8cc401ea0d314d2aa84bb29 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Valeria Cornelius verfasserin aut Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE. Peyer’s patch quercetin tight junction epithelial barrier Ussing chamber claudins Biology (General) Chemistry Linda Droessler verfasserin aut Salah Amasheh verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 3, p 1530 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:3, p 1530 https://doi.org/10.3390/ijms25031530 kostenfrei https://doaj.org/article/fb102ca6d8cc401ea0d314d2aa84bb29 kostenfrei https://www.mdpi.com/1422-0067/25/3/1530 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 3, p 1530
allfieldsGer	10.3390/ijms25031530 doi (DE-627)DOAJ094494142 (DE-599)DOAJfb102ca6d8cc401ea0d314d2aa84bb29 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Valeria Cornelius verfasserin aut Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE. Peyer’s patch quercetin tight junction epithelial barrier Ussing chamber claudins Biology (General) Chemistry Linda Droessler verfasserin aut Salah Amasheh verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 3, p 1530 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:3, p 1530 https://doi.org/10.3390/ijms25031530 kostenfrei https://doaj.org/article/fb102ca6d8cc401ea0d314d2aa84bb29 kostenfrei https://www.mdpi.com/1422-0067/25/3/1530 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 3, p 1530
allfieldsSound	10.3390/ijms25031530 doi (DE-627)DOAJ094494142 (DE-599)DOAJfb102ca6d8cc401ea0d314d2aa84bb29 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Valeria Cornelius verfasserin aut Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE. Peyer’s patch quercetin tight junction epithelial barrier Ussing chamber claudins Biology (General) Chemistry Linda Droessler verfasserin aut Salah Amasheh verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 3, p 1530 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:3, p 1530 https://doi.org/10.3390/ijms25031530 kostenfrei https://doaj.org/article/fb102ca6d8cc401ea0d314d2aa84bb29 kostenfrei https://www.mdpi.com/1422-0067/25/3/1530 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 3, p 1530
language	English
source	In International Journal of Molecular Sciences 25(2024), 3, p 1530 volume:25 year:2024 number:3, p 1530
sourceStr	In International Journal of Molecular Sciences 25(2024), 3, p 1530 volume:25 year:2024 number:3, p 1530
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topic_facet	Peyer’s patch quercetin tight junction epithelial barrier Ussing chamber claudins Biology (General) Chemistry
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container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Valeria Cornelius @@aut@@ Linda Droessler @@aut@@ Salah Amasheh @@aut@@
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author	Valeria Cornelius
spellingShingle	Valeria Cornelius misc QH301-705.5 misc QD1-999 misc Peyer’s patch misc quercetin misc tight junction misc epithelial barrier misc Ussing chamber misc claudins misc Biology (General) misc Chemistry Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches
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topic_title	QH301-705.5 QD1-999 Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches Peyer’s patch quercetin tight junction epithelial barrier Ussing chamber claudins
topic	misc QH301-705.5 misc QD1-999 misc Peyer’s patch misc quercetin misc tight junction misc epithelial barrier misc Ussing chamber misc claudins misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc Peyer’s patch misc quercetin misc tight junction misc epithelial barrier misc Ussing chamber misc claudins misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc Peyer’s patch misc quercetin misc tight junction misc epithelial barrier misc Ussing chamber misc claudins misc Biology (General) misc Chemistry
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title	Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches
ctrlnum	(DE-627)DOAJ094494142 (DE-599)DOAJfb102ca6d8cc401ea0d314d2aa84bb29
title_full	Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches
author_sort	Valeria Cornelius
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
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author_browse	Valeria Cornelius Linda Droessler Salah Amasheh
container_volume	25
class	QH301-705.5 QD1-999
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author-letter	Valeria Cornelius
doi_str_mv	10.3390/ijms25031530
author2-role	verfasserin
title_sort	quercetin improves barrier properties in porcine small intestine but not in peyer’s patches
callnumber	QH301-705.5
title_auth	Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches
abstract	Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE.
abstractGer	Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE.
abstract_unstemmed	Peyer’s patches (PPs) are part of the gut-associated lymphatic tissue (GALT) and represent the first line of the intestinal immunological defense. They consist of follicles with lymphocytes and an overlying subepithelial dome with dendritic cells and macrophages, and they are covered by the follicle-associated epithelium (FAE). A sealed paracellular pathway in the FAE is crucial for the controlled uptake of luminal antigens. Quercetin is the most abundant plant flavonoid and has a barrier-strengthening effect on tight junctions (TJs), a protein complex that regulates the paracellular pathway. In this study, we aimed to analyze the effect of quercetin on porcine PPs and the surrounding villus epithelium (VE). We incubated both tissue types for 4 h in Ussing chambers, recorded the transepithelial electrical resistance (TEER), and measured the unidirectional tracer flux of [<sup<3</sup<H]-mannitol. Subsequently, we analyzed the expression, protein amount, and localization of three TJ proteins, claudin 1, claudin 2, and claudin 4. In the PPs, we could not detect an effect of quercetin after 4 h, neither on TEER nor on the [<sup<3</sup<H]-mannitol flux. In the VE, quercetin led to a higher TEER value, while the [<sup<3</sup<H]-mannitol flux was unchanged. The pore-forming claudin 2 was decreased while the barrier-forming claudin 4 was increased and the expression was upregulated. Claudin 1 was unchanged and all claudins could be located in the paracellular membrane by immunofluorescence microscopy. Our study shows the barrier-strengthening effect of quercetin in porcine VE by claudin 4 upregulation and a claudin 2 decrease. Moreover, it underlines the different barrier properties of PPs compared to the VE.
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container_issue	3, p 1530
title_short	Quercetin Improves Barrier Properties in Porcine Small Intestine but Not in Peyer’s Patches
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