Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis

Most mammals tolerate exposure to hypobaric hypoxia poorly as it may affect multiple regulatory mechanisms and inhibit cell proliferation, promote apoptosis, limit tissue vascularization, and disrupt the acid–base equilibrium. Here, we quantified the functional state of germ cell development and dem...
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Autor*in:	Yun-Hua Ji [verfasserIn] Lin-Meng Wang [verfasserIn] Fu-Xun Zhang [verfasserIn] Hao-Zhong Hou [verfasserIn] Zhi-Rong Luo [verfasserIn] Qi Xue [verfasserIn] Man-Man Shi [verfasserIn] Yong Jiao [verfasserIn] Dong Cui [verfasserIn] Da-Li He [verfasserIn] Wei Xue [verfasserIn] Yu-qi Wen [verfasserIn] Qi-Sheng Tang [verfasserIn] Bo Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	cell atlas cell type hypobaric hypoxia single-cell RNA sequencing testis

Übergeordnetes Werk:	In: Frontiers in Cell and Developmental Biology - Frontiers Media S.A., 2014, 11(2023)
Übergeordnetes Werk:	volume:11 ; year:2023

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fcell.2023.1282119

Katalog-ID:	DOAJ094555575

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spelling	10.3389/fcell.2023.1282119 doi (DE-627)DOAJ094555575 (DE-599)DOAJ61e080b8ce26462f8531249a695e9841 DE-627 ger DE-627 rakwb eng QH301-705.5 Yun-Hua Ji verfasserin aut Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Most mammals tolerate exposure to hypobaric hypoxia poorly as it may affect multiple regulatory mechanisms and inhibit cell proliferation, promote apoptosis, limit tissue vascularization, and disrupt the acid–base equilibrium. Here, we quantified the functional state of germ cell development and demonstrated the interaction between the germ and somatic cells via single-cell RNA sequencing (scRNA-seq). The present study elucidated the regulatory effects of hypobaric hypoxia exposure on germ cell formation and sperm differentiation by applying enrichment analysis to genomic regions. Hypobaric hypoxia downregulates the genes controlling granule secretion and organic matter biosynthesis, upregulates tektin 1 (TEKT1) and kinesin family member 2C (KIF2C), and downregulates 60S ribosomal protein 11 (RPL11) and cilia- and flagella-associated protein 206 (CFAP206). Our research indicated that prosaposin-G protein-coupled receptor 37 (PSAP-GPR37) ligands mediate the damage to supporting cells caused by hypobaric hypoxic exposure. The present work revealed that hypoxia injures peritubular myoid (PTM) cells and spermatocytes in the S phase. It also showed that elongating spermatids promote maturation toward the G2 phase and increase their functional reserve for sperm–egg binding. The results of this study provide a theoretical basis for future investigations on prophylactic and therapeutic approaches toward protecting the reproductive system against the harmful effects of hypobaric hypoxic exposure. cell atlas cell type hypobaric hypoxia single-cell RNA sequencing testis Biology (General) Lin-Meng Wang verfasserin aut Fu-Xun Zhang verfasserin aut Hao-Zhong Hou verfasserin aut Zhi-Rong Luo verfasserin aut Qi Xue verfasserin aut Man-Man Shi verfasserin aut Yong Jiao verfasserin aut Dong Cui verfasserin aut Da-Li He verfasserin aut Wei Xue verfasserin aut Yu-qi Wen verfasserin aut Qi-Sheng Tang verfasserin aut Bo Zhang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 11(2023) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:11 year:2023 https://doi.org/10.3389/fcell.2023.1282119 kostenfrei https://doaj.org/article/61e080b8ce26462f8531249a695e9841 kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2023.1282119/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2023
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allfieldsSound	10.3389/fcell.2023.1282119 doi (DE-627)DOAJ094555575 (DE-599)DOAJ61e080b8ce26462f8531249a695e9841 DE-627 ger DE-627 rakwb eng QH301-705.5 Yun-Hua Ji verfasserin aut Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Most mammals tolerate exposure to hypobaric hypoxia poorly as it may affect multiple regulatory mechanisms and inhibit cell proliferation, promote apoptosis, limit tissue vascularization, and disrupt the acid–base equilibrium. Here, we quantified the functional state of germ cell development and demonstrated the interaction between the germ and somatic cells via single-cell RNA sequencing (scRNA-seq). The present study elucidated the regulatory effects of hypobaric hypoxia exposure on germ cell formation and sperm differentiation by applying enrichment analysis to genomic regions. Hypobaric hypoxia downregulates the genes controlling granule secretion and organic matter biosynthesis, upregulates tektin 1 (TEKT1) and kinesin family member 2C (KIF2C), and downregulates 60S ribosomal protein 11 (RPL11) and cilia- and flagella-associated protein 206 (CFAP206). Our research indicated that prosaposin-G protein-coupled receptor 37 (PSAP-GPR37) ligands mediate the damage to supporting cells caused by hypobaric hypoxic exposure. The present work revealed that hypoxia injures peritubular myoid (PTM) cells and spermatocytes in the S phase. It also showed that elongating spermatids promote maturation toward the G2 phase and increase their functional reserve for sperm–egg binding. The results of this study provide a theoretical basis for future investigations on prophylactic and therapeutic approaches toward protecting the reproductive system against the harmful effects of hypobaric hypoxic exposure. cell atlas cell type hypobaric hypoxia single-cell RNA sequencing testis Biology (General) Lin-Meng Wang verfasserin aut Fu-Xun Zhang verfasserin aut Hao-Zhong Hou verfasserin aut Zhi-Rong Luo verfasserin aut Qi Xue verfasserin aut Man-Man Shi verfasserin aut Yong Jiao verfasserin aut Dong Cui verfasserin aut Da-Li He verfasserin aut Wei Xue verfasserin aut Yu-qi Wen verfasserin aut Qi-Sheng Tang verfasserin aut Bo Zhang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 11(2023) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:11 year:2023 https://doi.org/10.3389/fcell.2023.1282119 kostenfrei https://doaj.org/article/61e080b8ce26462f8531249a695e9841 kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2023.1282119/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2023
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author	Yun-Hua Ji
spellingShingle	Yun-Hua Ji misc QH301-705.5 misc cell atlas misc cell type misc hypobaric misc hypoxia misc single-cell RNA sequencing misc testis misc Biology (General) Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis
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topic_title	QH301-705.5 Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis cell atlas cell type hypobaric hypoxia single-cell RNA sequencing testis
topic	misc QH301-705.5 misc cell atlas misc cell type misc hypobaric misc hypoxia misc single-cell RNA sequencing misc testis misc Biology (General)
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title	Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis
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title_sort	cascading effects of hypobaric hypoxia on the testis: insights from a single-cell rna sequencing analysis
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title_auth	Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis
abstract	Most mammals tolerate exposure to hypobaric hypoxia poorly as it may affect multiple regulatory mechanisms and inhibit cell proliferation, promote apoptosis, limit tissue vascularization, and disrupt the acid–base equilibrium. Here, we quantified the functional state of germ cell development and demonstrated the interaction between the germ and somatic cells via single-cell RNA sequencing (scRNA-seq). The present study elucidated the regulatory effects of hypobaric hypoxia exposure on germ cell formation and sperm differentiation by applying enrichment analysis to genomic regions. Hypobaric hypoxia downregulates the genes controlling granule secretion and organic matter biosynthesis, upregulates tektin 1 (TEKT1) and kinesin family member 2C (KIF2C), and downregulates 60S ribosomal protein 11 (RPL11) and cilia- and flagella-associated protein 206 (CFAP206). Our research indicated that prosaposin-G protein-coupled receptor 37 (PSAP-GPR37) ligands mediate the damage to supporting cells caused by hypobaric hypoxic exposure. The present work revealed that hypoxia injures peritubular myoid (PTM) cells and spermatocytes in the S phase. It also showed that elongating spermatids promote maturation toward the G2 phase and increase their functional reserve for sperm–egg binding. The results of this study provide a theoretical basis for future investigations on prophylactic and therapeutic approaches toward protecting the reproductive system against the harmful effects of hypobaric hypoxic exposure.
abstractGer	Most mammals tolerate exposure to hypobaric hypoxia poorly as it may affect multiple regulatory mechanisms and inhibit cell proliferation, promote apoptosis, limit tissue vascularization, and disrupt the acid–base equilibrium. Here, we quantified the functional state of germ cell development and demonstrated the interaction between the germ and somatic cells via single-cell RNA sequencing (scRNA-seq). The present study elucidated the regulatory effects of hypobaric hypoxia exposure on germ cell formation and sperm differentiation by applying enrichment analysis to genomic regions. Hypobaric hypoxia downregulates the genes controlling granule secretion and organic matter biosynthesis, upregulates tektin 1 (TEKT1) and kinesin family member 2C (KIF2C), and downregulates 60S ribosomal protein 11 (RPL11) and cilia- and flagella-associated protein 206 (CFAP206). Our research indicated that prosaposin-G protein-coupled receptor 37 (PSAP-GPR37) ligands mediate the damage to supporting cells caused by hypobaric hypoxic exposure. The present work revealed that hypoxia injures peritubular myoid (PTM) cells and spermatocytes in the S phase. It also showed that elongating spermatids promote maturation toward the G2 phase and increase their functional reserve for sperm–egg binding. The results of this study provide a theoretical basis for future investigations on prophylactic and therapeutic approaches toward protecting the reproductive system against the harmful effects of hypobaric hypoxic exposure.
abstract_unstemmed	Most mammals tolerate exposure to hypobaric hypoxia poorly as it may affect multiple regulatory mechanisms and inhibit cell proliferation, promote apoptosis, limit tissue vascularization, and disrupt the acid–base equilibrium. Here, we quantified the functional state of germ cell development and demonstrated the interaction between the germ and somatic cells via single-cell RNA sequencing (scRNA-seq). The present study elucidated the regulatory effects of hypobaric hypoxia exposure on germ cell formation and sperm differentiation by applying enrichment analysis to genomic regions. Hypobaric hypoxia downregulates the genes controlling granule secretion and organic matter biosynthesis, upregulates tektin 1 (TEKT1) and kinesin family member 2C (KIF2C), and downregulates 60S ribosomal protein 11 (RPL11) and cilia- and flagella-associated protein 206 (CFAP206). Our research indicated that prosaposin-G protein-coupled receptor 37 (PSAP-GPR37) ligands mediate the damage to supporting cells caused by hypobaric hypoxic exposure. The present work revealed that hypoxia injures peritubular myoid (PTM) cells and spermatocytes in the S phase. It also showed that elongating spermatids promote maturation toward the G2 phase and increase their functional reserve for sperm–egg binding. The results of this study provide a theoretical basis for future investigations on prophylactic and therapeutic approaches toward protecting the reproductive system against the harmful effects of hypobaric hypoxic exposure.
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title_short	Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis
url	https://doi.org/10.3389/fcell.2023.1282119 https://doaj.org/article/61e080b8ce26462f8531249a695e9841 https://www.frontiersin.org/articles/10.3389/fcell.2023.1282119/full https://doaj.org/toc/2296-634X
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up_date	2024-07-03T23:41:20.645Z
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Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis

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