Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds

Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity p...
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Gespeichert in:

Autor*in:	Mihaela Tociu [verfasserIn] Fulvia Manolache [verfasserIn] Brîndușa Bălănucă [verfasserIn] Alina Moroșan [verfasserIn] Raluca Stan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	total polyphenol content total antioxidant capacity bioactive compounds FT-ICR-MS waste recovery

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 28(2023), 21, p 7328
Übergeordnetes Werk:	volume:28 ; year:2023 ; number:21, p 7328

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules28217328

Katalog-ID:	DOAJ095450122

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allfields	10.3390/molecules28217328 doi (DE-627)DOAJ095450122 (DE-599)DOAJe1cd332370fc4852bbfb2d1def4b04fc DE-627 ger DE-627 rakwb eng QD241-441 Mihaela Tociu verfasserin aut Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity phases: young green leaves (YGL), green leaves (GL), mature green leaves (MGL), and yellow leaves (YL), using different solvents: ethanol (e), water (w), or water:ethanol (1:1 (<i<v</i</<i<v</i<)—m) by employing several methods: magnetic stirring (MS), ultrasound-assisted (UA), as well as maceration (M). The obtained extracts were quantitatively evaluated through spectrophotometric methods: Total Polyphenol Content (<i<TPC</i<-Folin–Ciocalteu assay) and Total Antioxidant Capacity (<i<TEAC</i< assay). Phytochemical screening by means of Fourier-Transform Ion–Cyclotron-Resonance High-Resolution Mass Spectrometry (FT-ICR-MS) indicated the presence of 40 compounds belonging to different phytochemical classes: phenolic acids, flavonoids, flavones, flavanones, flavonones, flavanols, vitamins, tereponoid, steroid, anthocyanidin, and other compounds. Based on <i<TPC</i< and <i<TEAC</i< assays, the water-ethanol mixture was found to be the proper extraction solvent, with the best results being obtained for YL plant material: 146.29 mg GAE/g DM (<i<TPC</i<) and 11.67 mM TE/g DM (<i<TEAC</i<). This type of extract may be used in various domains, such as the cosmetics industry, the biomedical field, and/or the design of functional foods, relying on their phytochemical composition. <i<Juglans regia</i< L. leaves extracts total polyphenol content total antioxidant capacity bioactive compounds FT-ICR-MS waste recovery Organic chemistry Fulvia Manolache verfasserin aut Brîndușa Bălănucă verfasserin aut Alina Moroșan verfasserin aut Raluca Stan verfasserin aut In Molecules MDPI AG, 2003 28(2023), 21, p 7328 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:21, p 7328 https://doi.org/10.3390/molecules28217328 kostenfrei https://doaj.org/article/e1cd332370fc4852bbfb2d1def4b04fc kostenfrei https://www.mdpi.com/1420-3049/28/21/7328 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 21, p 7328
spelling	10.3390/molecules28217328 doi (DE-627)DOAJ095450122 (DE-599)DOAJe1cd332370fc4852bbfb2d1def4b04fc DE-627 ger DE-627 rakwb eng QD241-441 Mihaela Tociu verfasserin aut Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity phases: young green leaves (YGL), green leaves (GL), mature green leaves (MGL), and yellow leaves (YL), using different solvents: ethanol (e), water (w), or water:ethanol (1:1 (<i<v</i</<i<v</i<)—m) by employing several methods: magnetic stirring (MS), ultrasound-assisted (UA), as well as maceration (M). The obtained extracts were quantitatively evaluated through spectrophotometric methods: Total Polyphenol Content (<i<TPC</i<-Folin–Ciocalteu assay) and Total Antioxidant Capacity (<i<TEAC</i< assay). Phytochemical screening by means of Fourier-Transform Ion–Cyclotron-Resonance High-Resolution Mass Spectrometry (FT-ICR-MS) indicated the presence of 40 compounds belonging to different phytochemical classes: phenolic acids, flavonoids, flavones, flavanones, flavonones, flavanols, vitamins, tereponoid, steroid, anthocyanidin, and other compounds. Based on <i<TPC</i< and <i<TEAC</i< assays, the water-ethanol mixture was found to be the proper extraction solvent, with the best results being obtained for YL plant material: 146.29 mg GAE/g DM (<i<TPC</i<) and 11.67 mM TE/g DM (<i<TEAC</i<). This type of extract may be used in various domains, such as the cosmetics industry, the biomedical field, and/or the design of functional foods, relying on their phytochemical composition. <i<Juglans regia</i< L. leaves extracts total polyphenol content total antioxidant capacity bioactive compounds FT-ICR-MS waste recovery Organic chemistry Fulvia Manolache verfasserin aut Brîndușa Bălănucă verfasserin aut Alina Moroșan verfasserin aut Raluca Stan verfasserin aut In Molecules MDPI AG, 2003 28(2023), 21, p 7328 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:21, p 7328 https://doi.org/10.3390/molecules28217328 kostenfrei https://doaj.org/article/e1cd332370fc4852bbfb2d1def4b04fc kostenfrei https://www.mdpi.com/1420-3049/28/21/7328 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 21, p 7328
allfields_unstemmed	10.3390/molecules28217328 doi (DE-627)DOAJ095450122 (DE-599)DOAJe1cd332370fc4852bbfb2d1def4b04fc DE-627 ger DE-627 rakwb eng QD241-441 Mihaela Tociu verfasserin aut Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity phases: young green leaves (YGL), green leaves (GL), mature green leaves (MGL), and yellow leaves (YL), using different solvents: ethanol (e), water (w), or water:ethanol (1:1 (<i<v</i</<i<v</i<)—m) by employing several methods: magnetic stirring (MS), ultrasound-assisted (UA), as well as maceration (M). The obtained extracts were quantitatively evaluated through spectrophotometric methods: Total Polyphenol Content (<i<TPC</i<-Folin–Ciocalteu assay) and Total Antioxidant Capacity (<i<TEAC</i< assay). Phytochemical screening by means of Fourier-Transform Ion–Cyclotron-Resonance High-Resolution Mass Spectrometry (FT-ICR-MS) indicated the presence of 40 compounds belonging to different phytochemical classes: phenolic acids, flavonoids, flavones, flavanones, flavonones, flavanols, vitamins, tereponoid, steroid, anthocyanidin, and other compounds. Based on <i<TPC</i< and <i<TEAC</i< assays, the water-ethanol mixture was found to be the proper extraction solvent, with the best results being obtained for YL plant material: 146.29 mg GAE/g DM (<i<TPC</i<) and 11.67 mM TE/g DM (<i<TEAC</i<). This type of extract may be used in various domains, such as the cosmetics industry, the biomedical field, and/or the design of functional foods, relying on their phytochemical composition. <i<Juglans regia</i< L. leaves extracts total polyphenol content total antioxidant capacity bioactive compounds FT-ICR-MS waste recovery Organic chemistry Fulvia Manolache verfasserin aut Brîndușa Bălănucă verfasserin aut Alina Moroșan verfasserin aut Raluca Stan verfasserin aut In Molecules MDPI AG, 2003 28(2023), 21, p 7328 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:21, p 7328 https://doi.org/10.3390/molecules28217328 kostenfrei https://doaj.org/article/e1cd332370fc4852bbfb2d1def4b04fc kostenfrei https://www.mdpi.com/1420-3049/28/21/7328 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 21, p 7328
allfieldsGer	10.3390/molecules28217328 doi (DE-627)DOAJ095450122 (DE-599)DOAJe1cd332370fc4852bbfb2d1def4b04fc DE-627 ger DE-627 rakwb eng QD241-441 Mihaela Tociu verfasserin aut Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity phases: young green leaves (YGL), green leaves (GL), mature green leaves (MGL), and yellow leaves (YL), using different solvents: ethanol (e), water (w), or water:ethanol (1:1 (<i<v</i</<i<v</i<)—m) by employing several methods: magnetic stirring (MS), ultrasound-assisted (UA), as well as maceration (M). The obtained extracts were quantitatively evaluated through spectrophotometric methods: Total Polyphenol Content (<i<TPC</i<-Folin–Ciocalteu assay) and Total Antioxidant Capacity (<i<TEAC</i< assay). Phytochemical screening by means of Fourier-Transform Ion–Cyclotron-Resonance High-Resolution Mass Spectrometry (FT-ICR-MS) indicated the presence of 40 compounds belonging to different phytochemical classes: phenolic acids, flavonoids, flavones, flavanones, flavonones, flavanols, vitamins, tereponoid, steroid, anthocyanidin, and other compounds. Based on <i<TPC</i< and <i<TEAC</i< assays, the water-ethanol mixture was found to be the proper extraction solvent, with the best results being obtained for YL plant material: 146.29 mg GAE/g DM (<i<TPC</i<) and 11.67 mM TE/g DM (<i<TEAC</i<). This type of extract may be used in various domains, such as the cosmetics industry, the biomedical field, and/or the design of functional foods, relying on their phytochemical composition. <i<Juglans regia</i< L. leaves extracts total polyphenol content total antioxidant capacity bioactive compounds FT-ICR-MS waste recovery Organic chemistry Fulvia Manolache verfasserin aut Brîndușa Bălănucă verfasserin aut Alina Moroșan verfasserin aut Raluca Stan verfasserin aut In Molecules MDPI AG, 2003 28(2023), 21, p 7328 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:21, p 7328 https://doi.org/10.3390/molecules28217328 kostenfrei https://doaj.org/article/e1cd332370fc4852bbfb2d1def4b04fc kostenfrei https://www.mdpi.com/1420-3049/28/21/7328 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 21, p 7328
allfieldsSound	10.3390/molecules28217328 doi (DE-627)DOAJ095450122 (DE-599)DOAJe1cd332370fc4852bbfb2d1def4b04fc DE-627 ger DE-627 rakwb eng QD241-441 Mihaela Tociu verfasserin aut Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity phases: young green leaves (YGL), green leaves (GL), mature green leaves (MGL), and yellow leaves (YL), using different solvents: ethanol (e), water (w), or water:ethanol (1:1 (<i<v</i</<i<v</i<)—m) by employing several methods: magnetic stirring (MS), ultrasound-assisted (UA), as well as maceration (M). The obtained extracts were quantitatively evaluated through spectrophotometric methods: Total Polyphenol Content (<i<TPC</i<-Folin–Ciocalteu assay) and Total Antioxidant Capacity (<i<TEAC</i< assay). Phytochemical screening by means of Fourier-Transform Ion–Cyclotron-Resonance High-Resolution Mass Spectrometry (FT-ICR-MS) indicated the presence of 40 compounds belonging to different phytochemical classes: phenolic acids, flavonoids, flavones, flavanones, flavonones, flavanols, vitamins, tereponoid, steroid, anthocyanidin, and other compounds. Based on <i<TPC</i< and <i<TEAC</i< assays, the water-ethanol mixture was found to be the proper extraction solvent, with the best results being obtained for YL plant material: 146.29 mg GAE/g DM (<i<TPC</i<) and 11.67 mM TE/g DM (<i<TEAC</i<). This type of extract may be used in various domains, such as the cosmetics industry, the biomedical field, and/or the design of functional foods, relying on their phytochemical composition. <i<Juglans regia</i< L. leaves extracts total polyphenol content total antioxidant capacity bioactive compounds FT-ICR-MS waste recovery Organic chemistry Fulvia Manolache verfasserin aut Brîndușa Bălănucă verfasserin aut Alina Moroșan verfasserin aut Raluca Stan verfasserin aut In Molecules MDPI AG, 2003 28(2023), 21, p 7328 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:28 year:2023 number:21, p 7328 https://doi.org/10.3390/molecules28217328 kostenfrei https://doaj.org/article/e1cd332370fc4852bbfb2d1def4b04fc kostenfrei https://www.mdpi.com/1420-3049/28/21/7328 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2023 21, p 7328
language	English
source	In Molecules 28(2023), 21, p 7328 volume:28 year:2023 number:21, p 7328
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topic_facet	<i<Juglans regia</i< L. leaves extracts total polyphenol content total antioxidant capacity bioactive compounds FT-ICR-MS waste recovery Organic chemistry
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authorswithroles_txt_mv	Mihaela Tociu @@aut@@ Fulvia Manolache @@aut@@ Brîndușa Bălănucă @@aut@@ Alina Moroșan @@aut@@ Raluca Stan @@aut@@
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issn	14203049
topic_title	QD241-441 Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds <i<Juglans regia</i< L. leaves extracts total polyphenol content total antioxidant capacity bioactive compounds FT-ICR-MS waste recovery
topic	misc QD241-441 misc <i<Juglans regia</i< L. leaves extracts misc total polyphenol content misc total antioxidant capacity misc bioactive compounds misc FT-ICR-MS misc waste recovery misc Organic chemistry
topic_unstemmed	misc QD241-441 misc <i<Juglans regia</i< L. leaves extracts misc total polyphenol content misc total antioxidant capacity misc bioactive compounds misc FT-ICR-MS misc waste recovery misc Organic chemistry
topic_browse	misc QD241-441 misc <i<Juglans regia</i< L. leaves extracts misc total polyphenol content misc total antioxidant capacity misc bioactive compounds misc FT-ICR-MS misc waste recovery misc Organic chemistry
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hierarchy_parent_title	Molecules
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title	Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds
ctrlnum	(DE-627)DOAJ095450122 (DE-599)DOAJe1cd332370fc4852bbfb2d1def4b04fc
title_full	Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds
author_sort	Mihaela Tociu
journal	Molecules
journalStr	Molecules
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author_browse	Mihaela Tociu Fulvia Manolache Brîndușa Bălănucă Alina Moroșan Raluca Stan
container_volume	28
class	QD241-441
format_se	Elektronische Aufsätze
author-letter	Mihaela Tociu
doi_str_mv	10.3390/molecules28217328
author2-role	verfasserin
title_sort	superior valorisation of <i<juglans regia</i< l. leaves of different maturity through the isolation of bioactive compounds
callnumber	QD241-441
title_auth	Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds
abstract	Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity phases: young green leaves (YGL), green leaves (GL), mature green leaves (MGL), and yellow leaves (YL), using different solvents: ethanol (e), water (w), or water:ethanol (1:1 (<i<v</i</<i<v</i<)—m) by employing several methods: magnetic stirring (MS), ultrasound-assisted (UA), as well as maceration (M). The obtained extracts were quantitatively evaluated through spectrophotometric methods: Total Polyphenol Content (<i<TPC</i<-Folin–Ciocalteu assay) and Total Antioxidant Capacity (<i<TEAC</i< assay). Phytochemical screening by means of Fourier-Transform Ion–Cyclotron-Resonance High-Resolution Mass Spectrometry (FT-ICR-MS) indicated the presence of 40 compounds belonging to different phytochemical classes: phenolic acids, flavonoids, flavones, flavanones, flavonones, flavanols, vitamins, tereponoid, steroid, anthocyanidin, and other compounds. Based on <i<TPC</i< and <i<TEAC</i< assays, the water-ethanol mixture was found to be the proper extraction solvent, with the best results being obtained for YL plant material: 146.29 mg GAE/g DM (<i<TPC</i<) and 11.67 mM TE/g DM (<i<TEAC</i<). This type of extract may be used in various domains, such as the cosmetics industry, the biomedical field, and/or the design of functional foods, relying on their phytochemical composition.
abstractGer	Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity phases: young green leaves (YGL), green leaves (GL), mature green leaves (MGL), and yellow leaves (YL), using different solvents: ethanol (e), water (w), or water:ethanol (1:1 (<i<v</i</<i<v</i<)—m) by employing several methods: magnetic stirring (MS), ultrasound-assisted (UA), as well as maceration (M). The obtained extracts were quantitatively evaluated through spectrophotometric methods: Total Polyphenol Content (<i<TPC</i<-Folin–Ciocalteu assay) and Total Antioxidant Capacity (<i<TEAC</i< assay). Phytochemical screening by means of Fourier-Transform Ion–Cyclotron-Resonance High-Resolution Mass Spectrometry (FT-ICR-MS) indicated the presence of 40 compounds belonging to different phytochemical classes: phenolic acids, flavonoids, flavones, flavanones, flavonones, flavanols, vitamins, tereponoid, steroid, anthocyanidin, and other compounds. Based on <i<TPC</i< and <i<TEAC</i< assays, the water-ethanol mixture was found to be the proper extraction solvent, with the best results being obtained for YL plant material: 146.29 mg GAE/g DM (<i<TPC</i<) and 11.67 mM TE/g DM (<i<TEAC</i<). This type of extract may be used in various domains, such as the cosmetics industry, the biomedical field, and/or the design of functional foods, relying on their phytochemical composition.
abstract_unstemmed	Extracts rich in bioactive compounds from natural sources have received great interest due to their great impact on human health. The aim of this research is focused on the obtaining and characterization of several extracts from <i<Juglans regia</i< L. leaves in four different maturity phases: young green leaves (YGL), green leaves (GL), mature green leaves (MGL), and yellow leaves (YL), using different solvents: ethanol (e), water (w), or water:ethanol (1:1 (<i<v</i</<i<v</i<)—m) by employing several methods: magnetic stirring (MS), ultrasound-assisted (UA), as well as maceration (M). The obtained extracts were quantitatively evaluated through spectrophotometric methods: Total Polyphenol Content (<i<TPC</i<-Folin–Ciocalteu assay) and Total Antioxidant Capacity (<i<TEAC</i< assay). Phytochemical screening by means of Fourier-Transform Ion–Cyclotron-Resonance High-Resolution Mass Spectrometry (FT-ICR-MS) indicated the presence of 40 compounds belonging to different phytochemical classes: phenolic acids, flavonoids, flavones, flavanones, flavonones, flavanols, vitamins, tereponoid, steroid, anthocyanidin, and other compounds. Based on <i<TPC</i< and <i<TEAC</i< assays, the water-ethanol mixture was found to be the proper extraction solvent, with the best results being obtained for YL plant material: 146.29 mg GAE/g DM (<i<TPC</i<) and 11.67 mM TE/g DM (<i<TEAC</i<). This type of extract may be used in various domains, such as the cosmetics industry, the biomedical field, and/or the design of functional foods, relying on their phytochemical composition.
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title_short	Superior Valorisation of <i<Juglans regia</i< L. Leaves of Different Maturity through the Isolation of Bioactive Compounds
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