CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy

The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, ute...
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Autor*in:	Xiaodan Li [verfasserIn] Xiaolei Yao [verfasserIn] Kang Li [verfasserIn] Jiahe Guo [verfasserIn] Kaiping Deng [verfasserIn] Zhipeng Liu [verfasserIn] Fan Yang [verfasserIn] Yixuan Fan [verfasserIn] Yingnan Yang [verfasserIn] Huabin Zhu [verfasserIn] Feng Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	endometrial receptivity CREB1 miR-134-5p endometrial stromal cells proliferation apoptosis

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 12(2023), 21, p 2554
Übergeordnetes Werk:	volume:12 ; year:2023 ; number:21, p 2554

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells12212554

Katalog-ID:	DOAJ095471359

Internformat


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520			\|a The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility.
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allfields	10.3390/cells12212554 doi (DE-627)DOAJ095471359 (DE-599)DOAJabda6d5c16154c66a71641c4fbb235dc DE-627 ger DE-627 rakwb eng QH573-671 Xiaodan Li verfasserin aut CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility. endometrial receptivity CREB1 miR-134-5p endometrial stromal cells proliferation apoptosis Cytology Xiaolei Yao verfasserin aut Kang Li verfasserin aut Jiahe Guo verfasserin aut Kaiping Deng verfasserin aut Zhipeng Liu verfasserin aut Fan Yang verfasserin aut Yixuan Fan verfasserin aut Yingnan Yang verfasserin aut Huabin Zhu verfasserin aut Feng Wang verfasserin aut In Cells MDPI AG, 2012 12(2023), 21, p 2554 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:12 year:2023 number:21, p 2554 https://doi.org/10.3390/cells12212554 kostenfrei https://doaj.org/article/abda6d5c16154c66a71641c4fbb235dc kostenfrei https://www.mdpi.com/2073-4409/12/21/2554 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 21, p 2554
spelling	10.3390/cells12212554 doi (DE-627)DOAJ095471359 (DE-599)DOAJabda6d5c16154c66a71641c4fbb235dc DE-627 ger DE-627 rakwb eng QH573-671 Xiaodan Li verfasserin aut CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility. endometrial receptivity CREB1 miR-134-5p endometrial stromal cells proliferation apoptosis Cytology Xiaolei Yao verfasserin aut Kang Li verfasserin aut Jiahe Guo verfasserin aut Kaiping Deng verfasserin aut Zhipeng Liu verfasserin aut Fan Yang verfasserin aut Yixuan Fan verfasserin aut Yingnan Yang verfasserin aut Huabin Zhu verfasserin aut Feng Wang verfasserin aut In Cells MDPI AG, 2012 12(2023), 21, p 2554 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:12 year:2023 number:21, p 2554 https://doi.org/10.3390/cells12212554 kostenfrei https://doaj.org/article/abda6d5c16154c66a71641c4fbb235dc kostenfrei https://www.mdpi.com/2073-4409/12/21/2554 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 21, p 2554
allfields_unstemmed	10.3390/cells12212554 doi (DE-627)DOAJ095471359 (DE-599)DOAJabda6d5c16154c66a71641c4fbb235dc DE-627 ger DE-627 rakwb eng QH573-671 Xiaodan Li verfasserin aut CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility. endometrial receptivity CREB1 miR-134-5p endometrial stromal cells proliferation apoptosis Cytology Xiaolei Yao verfasserin aut Kang Li verfasserin aut Jiahe Guo verfasserin aut Kaiping Deng verfasserin aut Zhipeng Liu verfasserin aut Fan Yang verfasserin aut Yixuan Fan verfasserin aut Yingnan Yang verfasserin aut Huabin Zhu verfasserin aut Feng Wang verfasserin aut In Cells MDPI AG, 2012 12(2023), 21, p 2554 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:12 year:2023 number:21, p 2554 https://doi.org/10.3390/cells12212554 kostenfrei https://doaj.org/article/abda6d5c16154c66a71641c4fbb235dc kostenfrei https://www.mdpi.com/2073-4409/12/21/2554 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 21, p 2554
allfieldsGer	10.3390/cells12212554 doi (DE-627)DOAJ095471359 (DE-599)DOAJabda6d5c16154c66a71641c4fbb235dc DE-627 ger DE-627 rakwb eng QH573-671 Xiaodan Li verfasserin aut CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility. endometrial receptivity CREB1 miR-134-5p endometrial stromal cells proliferation apoptosis Cytology Xiaolei Yao verfasserin aut Kang Li verfasserin aut Jiahe Guo verfasserin aut Kaiping Deng verfasserin aut Zhipeng Liu verfasserin aut Fan Yang verfasserin aut Yixuan Fan verfasserin aut Yingnan Yang verfasserin aut Huabin Zhu verfasserin aut Feng Wang verfasserin aut In Cells MDPI AG, 2012 12(2023), 21, p 2554 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:12 year:2023 number:21, p 2554 https://doi.org/10.3390/cells12212554 kostenfrei https://doaj.org/article/abda6d5c16154c66a71641c4fbb235dc kostenfrei https://www.mdpi.com/2073-4409/12/21/2554 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 21, p 2554
allfieldsSound	10.3390/cells12212554 doi (DE-627)DOAJ095471359 (DE-599)DOAJabda6d5c16154c66a71641c4fbb235dc DE-627 ger DE-627 rakwb eng QH573-671 Xiaodan Li verfasserin aut CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility. endometrial receptivity CREB1 miR-134-5p endometrial stromal cells proliferation apoptosis Cytology Xiaolei Yao verfasserin aut Kang Li verfasserin aut Jiahe Guo verfasserin aut Kaiping Deng verfasserin aut Zhipeng Liu verfasserin aut Fan Yang verfasserin aut Yixuan Fan verfasserin aut Yingnan Yang verfasserin aut Huabin Zhu verfasserin aut Feng Wang verfasserin aut In Cells MDPI AG, 2012 12(2023), 21, p 2554 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:12 year:2023 number:21, p 2554 https://doi.org/10.3390/cells12212554 kostenfrei https://doaj.org/article/abda6d5c16154c66a71641c4fbb235dc kostenfrei https://www.mdpi.com/2073-4409/12/21/2554 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 21, p 2554
language	English
source	In Cells 12(2023), 21, p 2554 volume:12 year:2023 number:21, p 2554
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author	Xiaodan Li
spellingShingle	Xiaodan Li misc QH573-671 misc endometrial receptivity misc CREB1 misc miR-134-5p misc endometrial stromal cells misc proliferation misc apoptosis misc Cytology CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy
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topic_title	QH573-671 CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy endometrial receptivity CREB1 miR-134-5p endometrial stromal cells proliferation apoptosis
topic	misc QH573-671 misc endometrial receptivity misc CREB1 misc miR-134-5p misc endometrial stromal cells misc proliferation misc apoptosis misc Cytology
topic_unstemmed	misc QH573-671 misc endometrial receptivity misc CREB1 misc miR-134-5p misc endometrial stromal cells misc proliferation misc apoptosis misc Cytology
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title	CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy
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title_sort	creb1 is involved in mir-134-5p-mediated endometrial stromal cell proliferation, apoptosis, and autophagy
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title_auth	CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy
abstract	The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility.
abstractGer	The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility.
abstract_unstemmed	The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and <i<CREB1</i< in the endometrium. In addition, <i<CREB1</i< overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that <i<CREB1</i< was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility.
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title_short	CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy
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Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (<i<CREB1</i<) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that <i<CREB1</i< significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. 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CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy

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