Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct

Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the...
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Autor*in:	Seon-Yeong Jeong [verfasserIn] Bong-Woo Park [verfasserIn] Jimin Kim [verfasserIn] Seulki Lee [verfasserIn] Haedeun You [verfasserIn] Joohyun Lee [verfasserIn] Susie Lee [verfasserIn] Jae-Hyun Park [verfasserIn] Jinju Kim [verfasserIn] Woosup Sim [verfasserIn] Kiwon Ban [verfasserIn] Joonghoon Park [verfasserIn] Hun-Jun Park [verfasserIn] Soo Kim [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Extracellular vesicles Induced Mesenchymal stem cells Myocardial infarction

Übergeordnetes Werk:	In: Journal of Nanobiotechnology - BMC, 2003, 22(2024), 1, Seite 20
Übergeordnetes Werk:	volume:22 ; year:2024 ; number:1 ; pages:20

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12951-024-02410-x

Katalog-ID:	DOAJ095851909

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520			\|a Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract
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allfields	10.1186/s12951-024-02410-x doi (DE-627)DOAJ095851909 (DE-599)DOAJ41fc4742d5164342bbde702b8db2be18 DE-627 ger DE-627 rakwb eng TP248.13-248.65 R855-855.5 Seon-Yeong Jeong verfasserin aut Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract Extracellular vesicles Induced Mesenchymal stem cells Myocardial infarction Biotechnology Medical technology Bong-Woo Park verfasserin aut Jimin Kim verfasserin aut Seulki Lee verfasserin aut Haedeun You verfasserin aut Joohyun Lee verfasserin aut Susie Lee verfasserin aut Jae-Hyun Park verfasserin aut Jinju Kim verfasserin aut Woosup Sim verfasserin aut Kiwon Ban verfasserin aut Joonghoon Park verfasserin aut Hun-Jun Park verfasserin aut Soo Kim verfasserin aut In Journal of Nanobiotechnology BMC, 2003 22(2024), 1, Seite 20 (DE-627)362770328 (DE-600)2100022-0 14773155 nnns volume:22 year:2024 number:1 pages:20 https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/article/41fc4742d5164342bbde702b8db2be18 kostenfrei https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/toc/1477-3155 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2024 1 20
spelling	10.1186/s12951-024-02410-x doi (DE-627)DOAJ095851909 (DE-599)DOAJ41fc4742d5164342bbde702b8db2be18 DE-627 ger DE-627 rakwb eng TP248.13-248.65 R855-855.5 Seon-Yeong Jeong verfasserin aut Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract Extracellular vesicles Induced Mesenchymal stem cells Myocardial infarction Biotechnology Medical technology Bong-Woo Park verfasserin aut Jimin Kim verfasserin aut Seulki Lee verfasserin aut Haedeun You verfasserin aut Joohyun Lee verfasserin aut Susie Lee verfasserin aut Jae-Hyun Park verfasserin aut Jinju Kim verfasserin aut Woosup Sim verfasserin aut Kiwon Ban verfasserin aut Joonghoon Park verfasserin aut Hun-Jun Park verfasserin aut Soo Kim verfasserin aut In Journal of Nanobiotechnology BMC, 2003 22(2024), 1, Seite 20 (DE-627)362770328 (DE-600)2100022-0 14773155 nnns volume:22 year:2024 number:1 pages:20 https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/article/41fc4742d5164342bbde702b8db2be18 kostenfrei https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/toc/1477-3155 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2024 1 20
allfields_unstemmed	10.1186/s12951-024-02410-x doi (DE-627)DOAJ095851909 (DE-599)DOAJ41fc4742d5164342bbde702b8db2be18 DE-627 ger DE-627 rakwb eng TP248.13-248.65 R855-855.5 Seon-Yeong Jeong verfasserin aut Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract Extracellular vesicles Induced Mesenchymal stem cells Myocardial infarction Biotechnology Medical technology Bong-Woo Park verfasserin aut Jimin Kim verfasserin aut Seulki Lee verfasserin aut Haedeun You verfasserin aut Joohyun Lee verfasserin aut Susie Lee verfasserin aut Jae-Hyun Park verfasserin aut Jinju Kim verfasserin aut Woosup Sim verfasserin aut Kiwon Ban verfasserin aut Joonghoon Park verfasserin aut Hun-Jun Park verfasserin aut Soo Kim verfasserin aut In Journal of Nanobiotechnology BMC, 2003 22(2024), 1, Seite 20 (DE-627)362770328 (DE-600)2100022-0 14773155 nnns volume:22 year:2024 number:1 pages:20 https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/article/41fc4742d5164342bbde702b8db2be18 kostenfrei https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/toc/1477-3155 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2024 1 20
allfieldsGer	10.1186/s12951-024-02410-x doi (DE-627)DOAJ095851909 (DE-599)DOAJ41fc4742d5164342bbde702b8db2be18 DE-627 ger DE-627 rakwb eng TP248.13-248.65 R855-855.5 Seon-Yeong Jeong verfasserin aut Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract Extracellular vesicles Induced Mesenchymal stem cells Myocardial infarction Biotechnology Medical technology Bong-Woo Park verfasserin aut Jimin Kim verfasserin aut Seulki Lee verfasserin aut Haedeun You verfasserin aut Joohyun Lee verfasserin aut Susie Lee verfasserin aut Jae-Hyun Park verfasserin aut Jinju Kim verfasserin aut Woosup Sim verfasserin aut Kiwon Ban verfasserin aut Joonghoon Park verfasserin aut Hun-Jun Park verfasserin aut Soo Kim verfasserin aut In Journal of Nanobiotechnology BMC, 2003 22(2024), 1, Seite 20 (DE-627)362770328 (DE-600)2100022-0 14773155 nnns volume:22 year:2024 number:1 pages:20 https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/article/41fc4742d5164342bbde702b8db2be18 kostenfrei https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/toc/1477-3155 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2024 1 20
allfieldsSound	10.1186/s12951-024-02410-x doi (DE-627)DOAJ095851909 (DE-599)DOAJ41fc4742d5164342bbde702b8db2be18 DE-627 ger DE-627 rakwb eng TP248.13-248.65 R855-855.5 Seon-Yeong Jeong verfasserin aut Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract Extracellular vesicles Induced Mesenchymal stem cells Myocardial infarction Biotechnology Medical technology Bong-Woo Park verfasserin aut Jimin Kim verfasserin aut Seulki Lee verfasserin aut Haedeun You verfasserin aut Joohyun Lee verfasserin aut Susie Lee verfasserin aut Jae-Hyun Park verfasserin aut Jinju Kim verfasserin aut Woosup Sim verfasserin aut Kiwon Ban verfasserin aut Joonghoon Park verfasserin aut Hun-Jun Park verfasserin aut Soo Kim verfasserin aut In Journal of Nanobiotechnology BMC, 2003 22(2024), 1, Seite 20 (DE-627)362770328 (DE-600)2100022-0 14773155 nnns volume:22 year:2024 number:1 pages:20 https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/article/41fc4742d5164342bbde702b8db2be18 kostenfrei https://doi.org/10.1186/s12951-024-02410-x kostenfrei https://doaj.org/toc/1477-3155 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2024 1 20
language	English
source	In Journal of Nanobiotechnology 22(2024), 1, Seite 20 volume:22 year:2024 number:1 pages:20
sourceStr	In Journal of Nanobiotechnology 22(2024), 1, Seite 20 volume:22 year:2024 number:1 pages:20
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Extracellular vesicles Induced Mesenchymal stem cells Myocardial infarction Biotechnology Medical technology
isfreeaccess_bool	true
container_title	Journal of Nanobiotechnology
authorswithroles_txt_mv	Seon-Yeong Jeong @@aut@@ Bong-Woo Park @@aut@@ Jimin Kim @@aut@@ Seulki Lee @@aut@@ Haedeun You @@aut@@ Joohyun Lee @@aut@@ Susie Lee @@aut@@ Jae-Hyun Park @@aut@@ Jinju Kim @@aut@@ Woosup Sim @@aut@@ Kiwon Ban @@aut@@ Joonghoon Park @@aut@@ Hun-Jun Park @@aut@@ Soo Kim @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	362770328
id	DOAJ095851909
language_de	englisch
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This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. 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callnumber-first	T - Technology
author	Seon-Yeong Jeong
spellingShingle	Seon-Yeong Jeong misc TP248.13-248.65 misc R855-855.5 misc Extracellular vesicles misc Induced Mesenchymal stem cells misc Myocardial infarction misc Biotechnology misc Medical technology Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct
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topic_title	TP248.13-248.65 R855-855.5 Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct Extracellular vesicles Induced Mesenchymal stem cells Myocardial infarction
topic	misc TP248.13-248.65 misc R855-855.5 misc Extracellular vesicles misc Induced Mesenchymal stem cells misc Myocardial infarction misc Biotechnology misc Medical technology
topic_unstemmed	misc TP248.13-248.65 misc R855-855.5 misc Extracellular vesicles misc Induced Mesenchymal stem cells misc Myocardial infarction misc Biotechnology misc Medical technology
topic_browse	misc TP248.13-248.65 misc R855-855.5 misc Extracellular vesicles misc Induced Mesenchymal stem cells misc Myocardial infarction misc Biotechnology misc Medical technology
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title	Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct
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title_full	Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct
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author_browse	Seon-Yeong Jeong Bong-Woo Park Jimin Kim Seulki Lee Haedeun You Joohyun Lee Susie Lee Jae-Hyun Park Jinju Kim Woosup Sim Kiwon Ban Joonghoon Park Hun-Jun Park Soo Kim
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title_sort	hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct
callnumber	TP248.13-248.65
title_auth	Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct
abstract	Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract
abstractGer	Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract
abstract_unstemmed	Abstract Background Myocardial infarction (MI), a representative form of ischemic heart disease, remains a huge burden worldwide. This study aimed to explore whether extracellular vesicles (EVs) secreted from hyaluronic acid (HA)-primed induced mesenchymal stem cells (HA-iMSC-EVs) could enhance the cardiac repair after MI. Results HA-iMSC-EVs showed typical characteristics for EVs such as morphology, size, and marker proteins expression. Compared with iMSC-EVs, HA-iMSC-EVs showed enhanced tube formation and survival against oxidative stress in endothelial cells, while reduced reactive oxygen species (ROS) generation in cardiomyocytes. In THP-1 macrophages, both types of EVs markedly reduced the expression of pro-inflammatory signaling players, whereas HA-iMSC-EVs were more potent in augmenting anti-inflammatory markers. A significant decrease of inflammasome proteins was observed in HA-iMSC-EV-treated THP-1. Further, phospho-SMAD2 as well as fibrosis markers in TGF-β1-stimulated cardiomyocytes were reduced in HA-iMSC-EVs treatment. Proteomic data showed that HA-iMSC-EVs were enriched with multiple pathways including immunity, extracellular matrix organization, angiogenesis, and cell cycle. The localization of HA-iMSC-EVs in myocardium was confirmed after delivery by either intravenous or intramyocardial route, with the latter increased intensity. Echocardiography revealed that intramyocardial HA-iMSC-EVs injections improved cardiac function and reduced adverse cardiac remodeling and necrotic size in MI heart. Histologically, MI hearts receiving HA-iMSC-EVs had increased capillary density and viable myocardium, while showed reduced fibrosis. Conclusions Our results suggest that HA-iMSC-EVs improve cardiac function by augmenting vessel growth, while reducing ROS generation, inflammation, and fibrosis in MI heart. Graphical Abstract
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title_short	Hyaluronic acid stimulation of stem cells for cardiac repair: a cell-free strategy for myocardial infarct
url	https://doi.org/10.1186/s12951-024-02410-x https://doaj.org/article/41fc4742d5164342bbde702b8db2be18 https://doaj.org/toc/1477-3155
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up_date	2024-07-03T16:57:56.522Z
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