Generation of human cerebral organoids with a structured outer subventricular zone

Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development...
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Autor*in:	Ryan M. Walsh [verfasserIn] Raffaele Luongo [verfasserIn] Elisa Giacomelli [verfasserIn] Gabriele Ciceri [verfasserIn] Chelsea Rittenhouse [verfasserIn] Antonietta Verrillo [verfasserIn] Maura Galimberti [verfasserIn] Vittoria Dickinson Bocchi [verfasserIn] Youjun Wu [verfasserIn] Nan Xu [verfasserIn] Simone Mosole [verfasserIn] James Muller [verfasserIn] Elena Vezzoli [verfasserIn] Johannes Jungverdorben [verfasserIn] Ting Zhou [verfasserIn] Roger A. Barker [verfasserIn] Elena Cattaneo [verfasserIn] Lorenz Studer [verfasserIn] Arianna Baggiolini [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	CP: Stem cell research CP: Neuroscience

Übergeordnetes Werk:	In: Cell Reports - Elsevier, 2015, 43(2024), 4, Seite 114031-
Übergeordnetes Werk:	volume:43 ; year:2024 ; number:4 ; pages:114031-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.celrep.2024.114031

Katalog-ID:	DOAJ095880577

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520			\|a Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP, LIFR, and HOPX, closely matching human fetal oRG. Finally, incorporating neural crest-derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely.
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spelling	10.1016/j.celrep.2024.114031 doi (DE-627)DOAJ095880577 (DE-599)DOAJ1bf423d6421944a39056287be8845aa4 DE-627 ger DE-627 rakwb eng QH301-705.5 Ryan M. Walsh verfasserin aut Generation of human cerebral organoids with a structured outer subventricular zone 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP, LIFR, and HOPX, closely matching human fetal oRG. Finally, incorporating neural crest-derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely. CP: Stem cell research CP: Neuroscience Biology (General) Raffaele Luongo verfasserin aut Elisa Giacomelli verfasserin aut Gabriele Ciceri verfasserin aut Chelsea Rittenhouse verfasserin aut Antonietta Verrillo verfasserin aut Maura Galimberti verfasserin aut Vittoria Dickinson Bocchi verfasserin aut Youjun Wu verfasserin aut Nan Xu verfasserin aut Simone Mosole verfasserin aut James Muller verfasserin aut Elena Vezzoli verfasserin aut Johannes Jungverdorben verfasserin aut Ting Zhou verfasserin aut Roger A. Barker verfasserin aut Elena Cattaneo verfasserin aut Lorenz Studer verfasserin aut Arianna Baggiolini verfasserin aut In Cell Reports Elsevier, 2015 43(2024), 4, Seite 114031- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:43 year:2024 number:4 pages:114031- https://doi.org/10.1016/j.celrep.2024.114031 kostenfrei https://doaj.org/article/1bf423d6421944a39056287be8845aa4 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124724003590 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 43 2024 4 114031-
allfields_unstemmed	10.1016/j.celrep.2024.114031 doi (DE-627)DOAJ095880577 (DE-599)DOAJ1bf423d6421944a39056287be8845aa4 DE-627 ger DE-627 rakwb eng QH301-705.5 Ryan M. Walsh verfasserin aut Generation of human cerebral organoids with a structured outer subventricular zone 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP, LIFR, and HOPX, closely matching human fetal oRG. Finally, incorporating neural crest-derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely. CP: Stem cell research CP: Neuroscience Biology (General) Raffaele Luongo verfasserin aut Elisa Giacomelli verfasserin aut Gabriele Ciceri verfasserin aut Chelsea Rittenhouse verfasserin aut Antonietta Verrillo verfasserin aut Maura Galimberti verfasserin aut Vittoria Dickinson Bocchi verfasserin aut Youjun Wu verfasserin aut Nan Xu verfasserin aut Simone Mosole verfasserin aut James Muller verfasserin aut Elena Vezzoli verfasserin aut Johannes Jungverdorben verfasserin aut Ting Zhou verfasserin aut Roger A. Barker verfasserin aut Elena Cattaneo verfasserin aut Lorenz Studer verfasserin aut Arianna Baggiolini verfasserin aut In Cell Reports Elsevier, 2015 43(2024), 4, Seite 114031- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:43 year:2024 number:4 pages:114031- https://doi.org/10.1016/j.celrep.2024.114031 kostenfrei https://doaj.org/article/1bf423d6421944a39056287be8845aa4 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124724003590 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 43 2024 4 114031-
allfieldsGer	10.1016/j.celrep.2024.114031 doi (DE-627)DOAJ095880577 (DE-599)DOAJ1bf423d6421944a39056287be8845aa4 DE-627 ger DE-627 rakwb eng QH301-705.5 Ryan M. Walsh verfasserin aut Generation of human cerebral organoids with a structured outer subventricular zone 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP, LIFR, and HOPX, closely matching human fetal oRG. Finally, incorporating neural crest-derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely. CP: Stem cell research CP: Neuroscience Biology (General) Raffaele Luongo verfasserin aut Elisa Giacomelli verfasserin aut Gabriele Ciceri verfasserin aut Chelsea Rittenhouse verfasserin aut Antonietta Verrillo verfasserin aut Maura Galimberti verfasserin aut Vittoria Dickinson Bocchi verfasserin aut Youjun Wu verfasserin aut Nan Xu verfasserin aut Simone Mosole verfasserin aut James Muller verfasserin aut Elena Vezzoli verfasserin aut Johannes Jungverdorben verfasserin aut Ting Zhou verfasserin aut Roger A. Barker verfasserin aut Elena Cattaneo verfasserin aut Lorenz Studer verfasserin aut Arianna Baggiolini verfasserin aut In Cell Reports Elsevier, 2015 43(2024), 4, Seite 114031- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:43 year:2024 number:4 pages:114031- https://doi.org/10.1016/j.celrep.2024.114031 kostenfrei https://doaj.org/article/1bf423d6421944a39056287be8845aa4 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124724003590 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 43 2024 4 114031-
allfieldsSound	10.1016/j.celrep.2024.114031 doi (DE-627)DOAJ095880577 (DE-599)DOAJ1bf423d6421944a39056287be8845aa4 DE-627 ger DE-627 rakwb eng QH301-705.5 Ryan M. Walsh verfasserin aut Generation of human cerebral organoids with a structured outer subventricular zone 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP, LIFR, and HOPX, closely matching human fetal oRG. Finally, incorporating neural crest-derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely. CP: Stem cell research CP: Neuroscience Biology (General) Raffaele Luongo verfasserin aut Elisa Giacomelli verfasserin aut Gabriele Ciceri verfasserin aut Chelsea Rittenhouse verfasserin aut Antonietta Verrillo verfasserin aut Maura Galimberti verfasserin aut Vittoria Dickinson Bocchi verfasserin aut Youjun Wu verfasserin aut Nan Xu verfasserin aut Simone Mosole verfasserin aut James Muller verfasserin aut Elena Vezzoli verfasserin aut Johannes Jungverdorben verfasserin aut Ting Zhou verfasserin aut Roger A. Barker verfasserin aut Elena Cattaneo verfasserin aut Lorenz Studer verfasserin aut Arianna Baggiolini verfasserin aut In Cell Reports Elsevier, 2015 43(2024), 4, Seite 114031- (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:43 year:2024 number:4 pages:114031- https://doi.org/10.1016/j.celrep.2024.114031 kostenfrei https://doaj.org/article/1bf423d6421944a39056287be8845aa4 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124724003590 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 43 2024 4 114031-
language	English
source	In Cell Reports 43(2024), 4, Seite 114031- volume:43 year:2024 number:4 pages:114031-
sourceStr	In Cell Reports 43(2024), 4, Seite 114031- volume:43 year:2024 number:4 pages:114031-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CP: Stem cell research CP: Neuroscience Biology (General)
isfreeaccess_bool	true
container_title	Cell Reports
authorswithroles_txt_mv	Ryan M. Walsh @@aut@@ Raffaele Luongo @@aut@@ Elisa Giacomelli @@aut@@ Gabriele Ciceri @@aut@@ Chelsea Rittenhouse @@aut@@ Antonietta Verrillo @@aut@@ Maura Galimberti @@aut@@ Vittoria Dickinson Bocchi @@aut@@ Youjun Wu @@aut@@ Nan Xu @@aut@@ Simone Mosole @@aut@@ James Muller @@aut@@ Elena Vezzoli @@aut@@ Johannes Jungverdorben @@aut@@ Ting Zhou @@aut@@ Roger A. Barker @@aut@@ Elena Cattaneo @@aut@@ Lorenz Studer @@aut@@ Arianna Baggiolini @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	684964562
id	DOAJ095880577
language_de	englisch
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Walsh</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Generation of human cerebral organoids with a structured outer subventricular zone</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2024</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. 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callnumber-first	Q - Science
author	Ryan M. Walsh
spellingShingle	Ryan M. Walsh misc QH301-705.5 misc CP: Stem cell research misc CP: Neuroscience misc Biology (General) Generation of human cerebral organoids with a structured outer subventricular zone
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topic_title	QH301-705.5 Generation of human cerebral organoids with a structured outer subventricular zone CP: Stem cell research CP: Neuroscience
topic	misc QH301-705.5 misc CP: Stem cell research misc CP: Neuroscience misc Biology (General)
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title	Generation of human cerebral organoids with a structured outer subventricular zone
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title_full	Generation of human cerebral organoids with a structured outer subventricular zone
author_sort	Ryan M. Walsh
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author_browse	Ryan M. Walsh Raffaele Luongo Elisa Giacomelli Gabriele Ciceri Chelsea Rittenhouse Antonietta Verrillo Maura Galimberti Vittoria Dickinson Bocchi Youjun Wu Nan Xu Simone Mosole James Muller Elena Vezzoli Johannes Jungverdorben Ting Zhou Roger A. Barker Elena Cattaneo Lorenz Studer Arianna Baggiolini
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author-letter	Ryan M. Walsh
doi_str_mv	10.1016/j.celrep.2024.114031
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title_sort	generation of human cerebral organoids with a structured outer subventricular zone
callnumber	QH301-705.5
title_auth	Generation of human cerebral organoids with a structured outer subventricular zone
abstract	Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP, LIFR, and HOPX, closely matching human fetal oRG. Finally, incorporating neural crest-derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely.
abstractGer	Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP, LIFR, and HOPX, closely matching human fetal oRG. Finally, incorporating neural crest-derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely.
abstract_unstemmed	Summary: Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ. The oSVZ comprises progenitor cells expressing specific oRG markers such as GFAP, LIFR, and HOPX, closely matching human fetal oRG. Finally, incorporating neural crest-derived LIF-producing cortical pericytes into cortical organoids recapitulates the effects of LIF treatment. These data indicate that increasing the cellular complexity of the organoid microenvironment promotes the emergence of oRG and supports a platform to study oRG in hPSC-derived brain organoids routinely.
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title_short	Generation of human cerebral organoids with a structured outer subventricular zone
url	https://doi.org/10.1016/j.celrep.2024.114031 https://doaj.org/article/1bf423d6421944a39056287be8845aa4 http://www.sciencedirect.com/science/article/pii/S2211124724003590 https://doaj.org/toc/2211-1247
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author2	Raffaele Luongo Elisa Giacomelli Gabriele Ciceri Chelsea Rittenhouse Antonietta Verrillo Maura Galimberti Vittoria Dickinson Bocchi Youjun Wu Nan Xu Simone Mosole James Muller Elena Vezzoli Johannes Jungverdorben Ting Zhou Roger A. Barker Elena Cattaneo Lorenz Studer Arianna Baggiolini
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up_date	2024-07-03T17:09:12.266Z
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Generation of human cerebral organoids with a structured outer subventricular zone

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