The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass

The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the en...
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Gespeichert in:

Autor*in:	Korbinian Sinzinger [verfasserIn] Ulrike Obst [verfasserIn] Samed Güner [verfasserIn] Manuel Döring [verfasserIn] Magdalena Haslbeck [verfasserIn] Doris Schieder [verfasserIn] Volker Sieber [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris

Übergeordnetes Werk:	In: Journal of Bioresources and Bioproducts - KeAi Communications Co., Ltd., 2018, 9(2024), 1, Seite 43-57
Übergeordnetes Werk:	volume:9 ; year:2024 ; number:1 ; pages:43-57

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.jobab.2023.12.005

Katalog-ID:	DOAJ096109718

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allfields	10.1016/j.jobab.2023.12.005 doi (DE-627)DOAJ096109718 (DE-599)DOAJ2e90d5a6f51a42cab34783315d2d8df9 DE-627 ger DE-627 rakwb eng QD415-436 Korbinian Sinzinger verfasserin aut The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the enzymes themselves comes with unavoidable impacts, e.g., the need to utilize secondary feedstocks. Here, we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation. We demonstrate these capabilities by producing a phytase secreted by P. pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex (BMGY) medium, with genetic expression conditions identified by high-throughput screening of a randomized secretion library. When our best-performing strain is grown in a fed-batch fermentation on BMGY, we reach over 7 000 U/mL in three days. Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris Biochemistry Ulrike Obst verfasserin aut Samed Güner verfasserin aut Manuel Döring verfasserin aut Magdalena Haslbeck verfasserin aut Doris Schieder verfasserin aut Volker Sieber verfasserin aut In Journal of Bioresources and Bioproducts KeAi Communications Co., Ltd., 2018 9(2024), 1, Seite 43-57 (DE-627)1040677878 23699698 nnns volume:9 year:2024 number:1 pages:43-57 https://doi.org/10.1016/j.jobab.2023.12.005 kostenfrei https://doaj.org/article/2e90d5a6f51a42cab34783315d2d8df9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2369969823000798 kostenfrei https://doaj.org/toc/2369-9698 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2024 1 43-57
spelling	10.1016/j.jobab.2023.12.005 doi (DE-627)DOAJ096109718 (DE-599)DOAJ2e90d5a6f51a42cab34783315d2d8df9 DE-627 ger DE-627 rakwb eng QD415-436 Korbinian Sinzinger verfasserin aut The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the enzymes themselves comes with unavoidable impacts, e.g., the need to utilize secondary feedstocks. Here, we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation. We demonstrate these capabilities by producing a phytase secreted by P. pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex (BMGY) medium, with genetic expression conditions identified by high-throughput screening of a randomized secretion library. When our best-performing strain is grown in a fed-batch fermentation on BMGY, we reach over 7 000 U/mL in three days. Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris Biochemistry Ulrike Obst verfasserin aut Samed Güner verfasserin aut Manuel Döring verfasserin aut Magdalena Haslbeck verfasserin aut Doris Schieder verfasserin aut Volker Sieber verfasserin aut In Journal of Bioresources and Bioproducts KeAi Communications Co., Ltd., 2018 9(2024), 1, Seite 43-57 (DE-627)1040677878 23699698 nnns volume:9 year:2024 number:1 pages:43-57 https://doi.org/10.1016/j.jobab.2023.12.005 kostenfrei https://doaj.org/article/2e90d5a6f51a42cab34783315d2d8df9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2369969823000798 kostenfrei https://doaj.org/toc/2369-9698 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2024 1 43-57
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allfieldsSound	10.1016/j.jobab.2023.12.005 doi (DE-627)DOAJ096109718 (DE-599)DOAJ2e90d5a6f51a42cab34783315d2d8df9 DE-627 ger DE-627 rakwb eng QD415-436 Korbinian Sinzinger verfasserin aut The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the enzymes themselves comes with unavoidable impacts, e.g., the need to utilize secondary feedstocks. Here, we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation. We demonstrate these capabilities by producing a phytase secreted by P. pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex (BMGY) medium, with genetic expression conditions identified by high-throughput screening of a randomized secretion library. When our best-performing strain is grown in a fed-batch fermentation on BMGY, we reach over 7 000 U/mL in three days. Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris Biochemistry Ulrike Obst verfasserin aut Samed Güner verfasserin aut Manuel Döring verfasserin aut Magdalena Haslbeck verfasserin aut Doris Schieder verfasserin aut Volker Sieber verfasserin aut In Journal of Bioresources and Bioproducts KeAi Communications Co., Ltd., 2018 9(2024), 1, Seite 43-57 (DE-627)1040677878 23699698 nnns volume:9 year:2024 number:1 pages:43-57 https://doi.org/10.1016/j.jobab.2023.12.005 kostenfrei https://doaj.org/article/2e90d5a6f51a42cab34783315d2d8df9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2369969823000798 kostenfrei https://doaj.org/toc/2369-9698 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2024 1 43-57
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authorswithroles_txt_mv	Korbinian Sinzinger @@aut@@ Ulrike Obst @@aut@@ Samed Güner @@aut@@ Manuel Döring @@aut@@ Magdalena Haslbeck @@aut@@ Doris Schieder @@aut@@ Volker Sieber @@aut@@
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author	Korbinian Sinzinger
spellingShingle	Korbinian Sinzinger misc QD415-436 misc Protein secretion misc Phytase misc Cyanobacterial biomass misc Fed-batch fermentation misc Pichia pastoris misc Biochemistry The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass
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topic_title	QD415-436 The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris
topic	misc QD415-436 misc Protein secretion misc Phytase misc Cyanobacterial biomass misc Fed-batch fermentation misc Pichia pastoris misc Biochemistry
topic_unstemmed	misc QD415-436 misc Protein secretion misc Phytase misc Cyanobacterial biomass misc Fed-batch fermentation misc Pichia pastoris misc Biochemistry
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title	The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass
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title_full	The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass
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title_sort	pichia pastoris enzyme production platform: from combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass
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title_auth	The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass
abstract	The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the enzymes themselves comes with unavoidable impacts, e.g., the need to utilize secondary feedstocks. Here, we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation. We demonstrate these capabilities by producing a phytase secreted by P. pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex (BMGY) medium, with genetic expression conditions identified by high-throughput screening of a randomized secretion library. When our best-performing strain is grown in a fed-batch fermentation on BMGY, we reach over 7 000 U/mL in three days.
abstractGer	The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the enzymes themselves comes with unavoidable impacts, e.g., the need to utilize secondary feedstocks. Here, we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation. We demonstrate these capabilities by producing a phytase secreted by P. pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex (BMGY) medium, with genetic expression conditions identified by high-throughput screening of a randomized secretion library. When our best-performing strain is grown in a fed-batch fermentation on BMGY, we reach over 7 000 U/mL in three days.
abstract_unstemmed	The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the enzymes themselves comes with unavoidable impacts, e.g., the need to utilize secondary feedstocks. Here, we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation. We demonstrate these capabilities by producing a phytase secreted by P. pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex (BMGY) medium, with genetic expression conditions identified by high-throughput screening of a randomized secretion library. When our best-performing strain is grown in a fed-batch fermentation on BMGY, we reach over 7 000 U/mL in three days.
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title_short	The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass
url	https://doi.org/10.1016/j.jobab.2023.12.005 https://doaj.org/article/2e90d5a6f51a42cab34783315d2d8df9 http://www.sciencedirect.com/science/article/pii/S2369969823000798 https://doaj.org/toc/2369-9698
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The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass

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