Temporal Quantitative Proteomic and Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells during All-<i<Trans</i<-Retinoic Acid-Induced Neuronal Differentiation

The human neuroblastoma cell lines SH-SY5Y and IMR-32 can be differentiated into neuron-like phenotypes through treatment with all-<i<trans</i<-retinoic acid (ATRA). After differentiation, these cell lines are extensively utilized as in vitro models to study various aspects of neuronal c...
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Gespeichert in:

Autor*in:	Thomas C. N. Leung [verfasserIn] Scott Ninghai Lu [verfasserIn] Cheuk Ning Chu [verfasserIn] Joy Lee [verfasserIn] Xingyu Liu [verfasserIn] Sai Ming Ngai [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	SH-SY5Y IMR-32 proteomics phosphoproteomics neuronal differentiation retinoic acid

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 25(2024), 2, p 1047
Übergeordnetes Werk:	volume:25 ; year:2024 ; number:2, p 1047

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms25021047

Katalog-ID:	DOAJ096184345

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callnumber-first	Q - Science
author	Thomas C. N. Leung
spellingShingle	Thomas C. N. Leung misc QH301-705.5 misc QD1-999 misc SH-SY5Y misc IMR-32 misc proteomics misc phosphoproteomics misc neuronal differentiation misc retinoic acid misc Biology (General) misc Chemistry Temporal Quantitative Proteomic and Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells during All-<i<Trans</i<-Retinoic Acid-Induced Neuronal Differentiation
authorStr	Thomas C. N. Leung
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topic_title	QH301-705.5 QD1-999 Temporal Quantitative Proteomic and Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells during All-<i<Trans</i<-Retinoic Acid-Induced Neuronal Differentiation SH-SY5Y IMR-32 proteomics phosphoproteomics neuronal differentiation retinoic acid
topic	misc QH301-705.5 misc QD1-999 misc SH-SY5Y misc IMR-32 misc proteomics misc phosphoproteomics misc neuronal differentiation misc retinoic acid misc Biology (General) misc Chemistry
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title	Temporal Quantitative Proteomic and Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells during All-<i<Trans</i<-Retinoic Acid-Induced Neuronal Differentiation
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title_full	Temporal Quantitative Proteomic and Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells during All-<i<Trans</i<-Retinoic Acid-Induced Neuronal Differentiation
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title_sort	temporal quantitative proteomic and phosphoproteomic profiling of sh-sy5y and imr-32 neuroblastoma cells during all-<i<trans</i<-retinoic acid-induced neuronal differentiation
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title_auth	Temporal Quantitative Proteomic and Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells during All-<i<Trans</i<-Retinoic Acid-Induced Neuronal Differentiation
abstract	The human neuroblastoma cell lines SH-SY5Y and IMR-32 can be differentiated into neuron-like phenotypes through treatment with all-<i<trans</i<-retinoic acid (ATRA). After differentiation, these cell lines are extensively utilized as in vitro models to study various aspects of neuronal cell biology. However, temporal and quantitative profiling of the proteome and phosphoproteome of SH-SY5Y and IMR-32 cells throughout ATRA-induced differentiation has been limited. Here, we performed relative quantification of the proteomes and phosphoproteomes of SH-SY5Y and IMR-32 cells at multiple time points during ATRA-induced differentiation. Relative quantification of proteins and phosphopeptides with subsequent gene ontology analysis revealed that several biological processes, including cytoskeleton organization, cell division, chaperone function and protein folding, and one-carbon metabolism, were associated with ATRA-induced differentiation in both cell lines. Furthermore, kinase-substrate enrichment analysis predicted altered activities of several kinases during differentiation. Among these, CDK5 exhibited increased activity, while CDK2 displayed reduced activity. The data presented serve as a valuable resource for investigating temporal protein and phosphoprotein abundance changes in SH-SY5Y and IMR-32 cells during ATRA-induced differentiation.
abstractGer	The human neuroblastoma cell lines SH-SY5Y and IMR-32 can be differentiated into neuron-like phenotypes through treatment with all-<i<trans</i<-retinoic acid (ATRA). After differentiation, these cell lines are extensively utilized as in vitro models to study various aspects of neuronal cell biology. However, temporal and quantitative profiling of the proteome and phosphoproteome of SH-SY5Y and IMR-32 cells throughout ATRA-induced differentiation has been limited. Here, we performed relative quantification of the proteomes and phosphoproteomes of SH-SY5Y and IMR-32 cells at multiple time points during ATRA-induced differentiation. Relative quantification of proteins and phosphopeptides with subsequent gene ontology analysis revealed that several biological processes, including cytoskeleton organization, cell division, chaperone function and protein folding, and one-carbon metabolism, were associated with ATRA-induced differentiation in both cell lines. Furthermore, kinase-substrate enrichment analysis predicted altered activities of several kinases during differentiation. Among these, CDK5 exhibited increased activity, while CDK2 displayed reduced activity. The data presented serve as a valuable resource for investigating temporal protein and phosphoprotein abundance changes in SH-SY5Y and IMR-32 cells during ATRA-induced differentiation.
abstract_unstemmed	The human neuroblastoma cell lines SH-SY5Y and IMR-32 can be differentiated into neuron-like phenotypes through treatment with all-<i<trans</i<-retinoic acid (ATRA). After differentiation, these cell lines are extensively utilized as in vitro models to study various aspects of neuronal cell biology. However, temporal and quantitative profiling of the proteome and phosphoproteome of SH-SY5Y and IMR-32 cells throughout ATRA-induced differentiation has been limited. Here, we performed relative quantification of the proteomes and phosphoproteomes of SH-SY5Y and IMR-32 cells at multiple time points during ATRA-induced differentiation. Relative quantification of proteins and phosphopeptides with subsequent gene ontology analysis revealed that several biological processes, including cytoskeleton organization, cell division, chaperone function and protein folding, and one-carbon metabolism, were associated with ATRA-induced differentiation in both cell lines. Furthermore, kinase-substrate enrichment analysis predicted altered activities of several kinases during differentiation. Among these, CDK5 exhibited increased activity, while CDK2 displayed reduced activity. The data presented serve as a valuable resource for investigating temporal protein and phosphoprotein abundance changes in SH-SY5Y and IMR-32 cells during ATRA-induced differentiation.
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title_short	Temporal Quantitative Proteomic and Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells during All-<i<Trans</i<-Retinoic Acid-Induced Neuronal Differentiation
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up_date	2024-07-03T18:45:09.328Z
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Temporal Quantitative Proteomic and Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells during All-<i<Trans</i<-Retinoic Acid-Induced Neuronal Differentiation

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