In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines

Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various hu...
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Autor*in:	Adel Sharaf Al-Zubairi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity

Übergeordnetes Werk:	In: Asian Pacific Journal of Cancer Biology - West Asia Organization for Cancer Prevention, 2018, 2(2017), 4, Seite 101-107
Übergeordnetes Werk:	volume:2 ; year:2017 ; number:4 ; pages:101-107

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.31557/apjcb.2017.2.4.101-107

Katalog-ID:	DOAJ096244291

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allfields	10.31557/apjcb.2017.2.4.101-107 doi (DE-627)DOAJ096244291 (DE-599)DOAJ8df9fe8a1a5e40c9b84326c66e3bc30f DE-627 ger DE-627 rakwb eng QH301-705.5 Adel Sharaf Al-Zubairi verfasserin aut In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines. zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity Biology (General) In Asian Pacific Journal of Cancer Biology West Asia Organization for Cancer Prevention, 2018 2(2017), 4, Seite 101-107 (DE-627)1696065542 25384635 nnns volume:2 year:2017 number:4 pages:101-107 https://doi.org/10.31557/apjcb.2017.2.4.101-107 kostenfrei https://doaj.org/article/8df9fe8a1a5e40c9b84326c66e3bc30f kostenfrei http://www.waocp.com/journal/index.php/apjcb/article/view/226 kostenfrei https://doaj.org/toc/2538-4635 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2017 4 101-107
spelling	10.31557/apjcb.2017.2.4.101-107 doi (DE-627)DOAJ096244291 (DE-599)DOAJ8df9fe8a1a5e40c9b84326c66e3bc30f DE-627 ger DE-627 rakwb eng QH301-705.5 Adel Sharaf Al-Zubairi verfasserin aut In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines. zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity Biology (General) In Asian Pacific Journal of Cancer Biology West Asia Organization for Cancer Prevention, 2018 2(2017), 4, Seite 101-107 (DE-627)1696065542 25384635 nnns volume:2 year:2017 number:4 pages:101-107 https://doi.org/10.31557/apjcb.2017.2.4.101-107 kostenfrei https://doaj.org/article/8df9fe8a1a5e40c9b84326c66e3bc30f kostenfrei http://www.waocp.com/journal/index.php/apjcb/article/view/226 kostenfrei https://doaj.org/toc/2538-4635 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2017 4 101-107
allfields_unstemmed	10.31557/apjcb.2017.2.4.101-107 doi (DE-627)DOAJ096244291 (DE-599)DOAJ8df9fe8a1a5e40c9b84326c66e3bc30f DE-627 ger DE-627 rakwb eng QH301-705.5 Adel Sharaf Al-Zubairi verfasserin aut In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines. zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity Biology (General) In Asian Pacific Journal of Cancer Biology West Asia Organization for Cancer Prevention, 2018 2(2017), 4, Seite 101-107 (DE-627)1696065542 25384635 nnns volume:2 year:2017 number:4 pages:101-107 https://doi.org/10.31557/apjcb.2017.2.4.101-107 kostenfrei https://doaj.org/article/8df9fe8a1a5e40c9b84326c66e3bc30f kostenfrei http://www.waocp.com/journal/index.php/apjcb/article/view/226 kostenfrei https://doaj.org/toc/2538-4635 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2017 4 101-107
allfieldsGer	10.31557/apjcb.2017.2.4.101-107 doi (DE-627)DOAJ096244291 (DE-599)DOAJ8df9fe8a1a5e40c9b84326c66e3bc30f DE-627 ger DE-627 rakwb eng QH301-705.5 Adel Sharaf Al-Zubairi verfasserin aut In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines. zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity Biology (General) In Asian Pacific Journal of Cancer Biology West Asia Organization for Cancer Prevention, 2018 2(2017), 4, Seite 101-107 (DE-627)1696065542 25384635 nnns volume:2 year:2017 number:4 pages:101-107 https://doi.org/10.31557/apjcb.2017.2.4.101-107 kostenfrei https://doaj.org/article/8df9fe8a1a5e40c9b84326c66e3bc30f kostenfrei http://www.waocp.com/journal/index.php/apjcb/article/view/226 kostenfrei https://doaj.org/toc/2538-4635 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2017 4 101-107
allfieldsSound	10.31557/apjcb.2017.2.4.101-107 doi (DE-627)DOAJ096244291 (DE-599)DOAJ8df9fe8a1a5e40c9b84326c66e3bc30f DE-627 ger DE-627 rakwb eng QH301-705.5 Adel Sharaf Al-Zubairi verfasserin aut In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines. zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity Biology (General) In Asian Pacific Journal of Cancer Biology West Asia Organization for Cancer Prevention, 2018 2(2017), 4, Seite 101-107 (DE-627)1696065542 25384635 nnns volume:2 year:2017 number:4 pages:101-107 https://doi.org/10.31557/apjcb.2017.2.4.101-107 kostenfrei https://doaj.org/article/8df9fe8a1a5e40c9b84326c66e3bc30f kostenfrei http://www.waocp.com/journal/index.php/apjcb/article/view/226 kostenfrei https://doaj.org/toc/2538-4635 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2017 4 101-107
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In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. 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callnumber-first	Q - Science
author	Adel Sharaf Al-Zubairi
spellingShingle	Adel Sharaf Al-Zubairi misc QH301-705.5 misc zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity misc Biology (General) In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines
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topic_title	QH301-705.5 In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity
topic	misc QH301-705.5 misc zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc zerumbone- cisplatin- cas- mni- cho cell lines- genotoxicity misc Biology (General)
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title	In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines
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title_full	In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines
author_sort	Adel Sharaf Al-Zubairi
journal	Asian Pacific Journal of Cancer Biology
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doi_str_mv	10.31557/apjcb.2017.2.4.101-107
title_sort	in vitro genotoxic effects of zerumbone and cisplatin combination in cho cell lines
callnumber	QH301-705.5
title_auth	In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines
abstract	Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines.
abstractGer	Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines.
abstract_unstemmed	Objective: Several natural products are being increasingly used in the treatment of cancer to minimize the adverse side effects of cancer chemotherapy. Zerumbone (ZER), the sesquiterpene derived from Zingiber zerumbet Smith, has been reported to have an in vitro anticancer effects against various human tumour cells as well as in vivo against a number of induced malignancies in mice. Previously we have reported the genotoxic effects of ZER in vitro against CHO cell lines. Material and Method: The aim of this study was to investigate the genotoxic effects of the combination of ZER along with cisplatin in CHO cells. Two cytogenetic endpoints were used, namely Chromosomal Aberrations assay (CA) and Micronucleus test (MN). Both cytogenetic endpoints were performed without any metabolic activation. Result: ZER treated cultures showed the significant increase in the frequency of the chromosome aberrations and MN induction. In CA assay, marked changes have been observed after co-treatment of CHO cell lines with different concentration of ZER along with 5 µM Cisplatin when compared to ZER treatment alone, suggesting a possible synergistic genetoxic effects. Whereas, treatment of CHO cell lines with different concentrations of ZER along with 2.5 µM Cisplatin was found to reduce chromosomal aberrations, suggesting an antagonistic genotoxic effect. On the other hand, in MN induction test, co-treatment of CHO cell lines with both 2.5 µM and 5 µM Cisplatin and different concentration of ZER found to reduce the genotoxic effects compared to the 2.5 µM and 5 µM Cisplatin alone suggesting an antagonistic genotoxic effect. Conclusion: The genotoxic effects of combined low concentrations of Cisplatin with different concentrations of ZER could have an antagonist genotoxic potential in vitro in CHO cell lines.
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title_short	In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines
url	https://doi.org/10.31557/apjcb.2017.2.4.101-107 https://doaj.org/article/8df9fe8a1a5e40c9b84326c66e3bc30f http://www.waocp.com/journal/index.php/apjcb/article/view/226 https://doaj.org/toc/2538-4635
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doi_str	10.31557/apjcb.2017.2.4.101-107
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up_date	2024-07-03T19:04:44.298Z
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In Vitro Genotoxic Effects of Zerumbone and Cisplatin Combination in CHO Cell Lines

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