Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes
It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial s...
Ausführliche Beschreibung
Autor*in: |
Chiara Andolina [verfasserIn] Wouter Graumans [verfasserIn] Moussa Guelbeogo [verfasserIn] Geert-Jan van Gemert [verfasserIn] Jordache Ramijth [verfasserIn] Soré Harouna [verfasserIn] Zongo Soumanaba [verfasserIn] Rianne Stoter [verfasserIn] Marga Vegte-Bolmer [verfasserIn] Martina Pangos [verfasserIn] Photini Sinnis [verfasserIn] Katharine Collins [verfasserIn] Sarah G Staedke [verfasserIn] Alfred B Tiono [verfasserIn] Chris Drakeley [verfasserIn] Kjerstin Lanke [verfasserIn] Teun Bousema [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2024 |
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Übergeordnetes Werk: |
In: eLife - eLife Sciences Publications Ltd, 2013, 12(2024) |
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Übergeordnetes Werk: |
volume:12 ; year:2024 |
Links: |
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DOI / URN: |
10.7554/eLife.90989 |
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Katalog-ID: |
DOAJ097215899 |
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520 | |a It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. | ||
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10.7554/eLife.90989 doi (DE-627)DOAJ097215899 (DE-599)DOAJbe98e34cc5dd4122ad803aa3d9086ded DE-627 ger DE-627 rakwb eng QH301-705.5 Chiara Andolina verfasserin aut Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. gametocyte sporozoite anopheles sporogony expelling Medicine R Science Q Biology (General) Wouter Graumans verfasserin aut Moussa Guelbeogo verfasserin aut Geert-Jan van Gemert verfasserin aut Jordache Ramijth verfasserin aut Soré Harouna verfasserin aut Zongo Soumanaba verfasserin aut Rianne Stoter verfasserin aut Marga Vegte-Bolmer verfasserin aut Martina Pangos verfasserin aut Photini Sinnis verfasserin aut Katharine Collins verfasserin aut Sarah G Staedke verfasserin aut Alfred B Tiono verfasserin aut Chris Drakeley verfasserin aut Kjerstin Lanke verfasserin aut Teun Bousema verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 12(2024) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:12 year:2024 https://doi.org/10.7554/eLife.90989 kostenfrei https://doaj.org/article/be98e34cc5dd4122ad803aa3d9086ded kostenfrei https://elifesciences.org/articles/90989 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 |
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10.7554/eLife.90989 doi (DE-627)DOAJ097215899 (DE-599)DOAJbe98e34cc5dd4122ad803aa3d9086ded DE-627 ger DE-627 rakwb eng QH301-705.5 Chiara Andolina verfasserin aut Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. gametocyte sporozoite anopheles sporogony expelling Medicine R Science Q Biology (General) Wouter Graumans verfasserin aut Moussa Guelbeogo verfasserin aut Geert-Jan van Gemert verfasserin aut Jordache Ramijth verfasserin aut Soré Harouna verfasserin aut Zongo Soumanaba verfasserin aut Rianne Stoter verfasserin aut Marga Vegte-Bolmer verfasserin aut Martina Pangos verfasserin aut Photini Sinnis verfasserin aut Katharine Collins verfasserin aut Sarah G Staedke verfasserin aut Alfred B Tiono verfasserin aut Chris Drakeley verfasserin aut Kjerstin Lanke verfasserin aut Teun Bousema verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 12(2024) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:12 year:2024 https://doi.org/10.7554/eLife.90989 kostenfrei https://doaj.org/article/be98e34cc5dd4122ad803aa3d9086ded kostenfrei https://elifesciences.org/articles/90989 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 |
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10.7554/eLife.90989 doi (DE-627)DOAJ097215899 (DE-599)DOAJbe98e34cc5dd4122ad803aa3d9086ded DE-627 ger DE-627 rakwb eng QH301-705.5 Chiara Andolina verfasserin aut Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. gametocyte sporozoite anopheles sporogony expelling Medicine R Science Q Biology (General) Wouter Graumans verfasserin aut Moussa Guelbeogo verfasserin aut Geert-Jan van Gemert verfasserin aut Jordache Ramijth verfasserin aut Soré Harouna verfasserin aut Zongo Soumanaba verfasserin aut Rianne Stoter verfasserin aut Marga Vegte-Bolmer verfasserin aut Martina Pangos verfasserin aut Photini Sinnis verfasserin aut Katharine Collins verfasserin aut Sarah G Staedke verfasserin aut Alfred B Tiono verfasserin aut Chris Drakeley verfasserin aut Kjerstin Lanke verfasserin aut Teun Bousema verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 12(2024) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:12 year:2024 https://doi.org/10.7554/eLife.90989 kostenfrei https://doaj.org/article/be98e34cc5dd4122ad803aa3d9086ded kostenfrei https://elifesciences.org/articles/90989 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 |
allfieldsGer |
10.7554/eLife.90989 doi (DE-627)DOAJ097215899 (DE-599)DOAJbe98e34cc5dd4122ad803aa3d9086ded DE-627 ger DE-627 rakwb eng QH301-705.5 Chiara Andolina verfasserin aut Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. gametocyte sporozoite anopheles sporogony expelling Medicine R Science Q Biology (General) Wouter Graumans verfasserin aut Moussa Guelbeogo verfasserin aut Geert-Jan van Gemert verfasserin aut Jordache Ramijth verfasserin aut Soré Harouna verfasserin aut Zongo Soumanaba verfasserin aut Rianne Stoter verfasserin aut Marga Vegte-Bolmer verfasserin aut Martina Pangos verfasserin aut Photini Sinnis verfasserin aut Katharine Collins verfasserin aut Sarah G Staedke verfasserin aut Alfred B Tiono verfasserin aut Chris Drakeley verfasserin aut Kjerstin Lanke verfasserin aut Teun Bousema verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 12(2024) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:12 year:2024 https://doi.org/10.7554/eLife.90989 kostenfrei https://doaj.org/article/be98e34cc5dd4122ad803aa3d9086ded kostenfrei https://elifesciences.org/articles/90989 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 |
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10.7554/eLife.90989 doi (DE-627)DOAJ097215899 (DE-599)DOAJbe98e34cc5dd4122ad803aa3d9086ded DE-627 ger DE-627 rakwb eng QH301-705.5 Chiara Andolina verfasserin aut Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. gametocyte sporozoite anopheles sporogony expelling Medicine R Science Q Biology (General) Wouter Graumans verfasserin aut Moussa Guelbeogo verfasserin aut Geert-Jan van Gemert verfasserin aut Jordache Ramijth verfasserin aut Soré Harouna verfasserin aut Zongo Soumanaba verfasserin aut Rianne Stoter verfasserin aut Marga Vegte-Bolmer verfasserin aut Martina Pangos verfasserin aut Photini Sinnis verfasserin aut Katharine Collins verfasserin aut Sarah G Staedke verfasserin aut Alfred B Tiono verfasserin aut Chris Drakeley verfasserin aut Kjerstin Lanke verfasserin aut Teun Bousema verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 12(2024) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:12 year:2024 https://doi.org/10.7554/eLife.90989 kostenfrei https://doaj.org/article/be98e34cc5dd4122ad803aa3d9086ded kostenfrei https://elifesciences.org/articles/90989 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2024 |
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Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes |
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Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes |
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Chiara Andolina |
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eLife |
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Chiara Andolina Wouter Graumans Moussa Guelbeogo Geert-Jan van Gemert Jordache Ramijth Soré Harouna Zongo Soumanaba Rianne Stoter Marga Vegte-Bolmer Martina Pangos Photini Sinnis Katharine Collins Sarah G Staedke Alfred B Tiono Chris Drakeley Kjerstin Lanke Teun Bousema |
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Chiara Andolina |
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quantification of sporozoite expelling by anopheles mosquitoes infected with laboratory and naturally circulating p. falciparum gametocytes |
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QH301-705.5 |
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Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes |
abstract |
It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. |
abstractGer |
It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. |
abstract_unstemmed |
It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. In laboratory conditions, higher total sporozoite burden was associated with shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of infected Anopheles stephensi mosquitoes expelled sporozoites into artificial skin with a median of 136 expelled sporozoites (interquartile range [IQR], 34–501). There was a strong positive correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.8; p<0.0001) and a weaker positive correlation between salivary gland sporozoite load and number of sporozoites expelled (ρ = 0.35; p=0.0002). In Burkina Faso, Anopheles coluzzii mosquitoes were infected by natural gametocyte carriers. Among salivary gland sporozoite positive mosquitoes, 89% (33/37) expelled sporozoites with a median of 1035 expelled sporozoites (IQR, 171–2969). Again, we observed a strong correlation between ruptured oocyst number and salivary gland sporozoite load (ρ = 0.9; p<0.0001) and a positive correlation between salivary gland sporozoite load and the number of sporozoites expelled (ρ = 0.7; p<0.0001). Several mosquitoes expelled multiple parasite clones during probing. Whilst sporozoite expelling was regularly observed from mosquitoes with low infection burdens, our findings indicate that mosquito infection burden is positively associated with the number of expelled sporozoites. Future work is required to determine the direct implications of these findings for transmission potential. |
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title_short |
Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes |
url |
https://doi.org/10.7554/eLife.90989 https://doaj.org/article/be98e34cc5dd4122ad803aa3d9086ded https://elifesciences.org/articles/90989 https://doaj.org/toc/2050-084X |
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Wouter Graumans Moussa Guelbeogo Geert-Jan van Gemert Jordache Ramijth Soré Harouna Zongo Soumanaba Rianne Stoter Marga Vegte-Bolmer Martina Pangos Photini Sinnis Katharine Collins Sarah G Staedke Alfred B Tiono Chris Drakeley Kjerstin Lanke Teun Bousema |
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Wouter Graumans Moussa Guelbeogo Geert-Jan van Gemert Jordache Ramijth Soré Harouna Zongo Soumanaba Rianne Stoter Marga Vegte-Bolmer Martina Pangos Photini Sinnis Katharine Collins Sarah G Staedke Alfred B Tiono Chris Drakeley Kjerstin Lanke Teun Bousema |
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up_date |
2024-07-04T00:15:55.976Z |
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